Comparative analysis of virus-host interactomes with a mammalian high- throughput protein complementation assay based on Gaussia princeps luciferase
Identifieur interne : 000262 ( Main/Exploration ); précédent : 000261; suivant : 000263Comparative analysis of virus-host interactomes with a mammalian high- throughput protein complementation assay based on Gaussia princeps luciferase
Auteurs : Grégory Neveu ; Patricia Cassonnet ; Pierre-Olivier Vidalain ; Caroline Rolloy ; José Mendoza ; Louis Jones [France] ; Frédéric Tangy ; Mandy Muller ; Caroline Demeret ; Lionel Tafforeau [France] ; Vincent Lotteau [France] ; Chantal Rabourdin-Combe [France] ; Gilles Travé [France] ; Amélie Dricot [États-Unis] ; David E. Hill [États-Unis] ; Marc Vidal [États-Unis] ; Michel Favre ; Yves JacobSource :
- Methods (San Diego, Calif.) [ 1046-2023 ] ; 2012.
Abstract
Comparative interactomics is a strategy for inferring potential interactions among orthologous proteins or “iginterologs”. Herein we focus, in contrast to standard homology-based inference, on the divergence of protein interaction profiles among closely related organisms, showing that the approach can correlate specific traits to phenotypic differences. As a model, this new comparative interactomic approach was applied at a large scale to human papillomaviruses (HPVs) proteins. The oncogenic potential of HPVs is mainly determined by the E6 and E7 early proteins. We have mapped and overlapped the virus-host protein interaction networks of E6 and E7 proteins from 11 distinct HPV genotypes, selected for their different tropisms and pathologies. We generated robust and comprehensive datasets by combining two orthogonal protein interation assays: yeast two-hybrid (Y2H), and our recently described “High-Throughput
Url:
DOI: 10.1016/j.ymeth.2012.07.029
PubMed: 22898364
PubMed Central: 3546263
Affiliations:
- France, États-Unis
- Alsace (région administrative), Auvergne-Rhône-Alpes, Grand Est, Massachusetts, Rhône-Alpes, Île-de-France
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Le document en format XML
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<front><div type="abstract" xml:lang="en"><p id="P2">Comparative interactomics is a strategy for inferring potential interactions among orthologous proteins or “iginterologs”. Herein we focus, in contrast to standard homology-based inference, on the divergence of protein interaction profiles among closely related organisms, showing that the approach can correlate specific traits to phenotypic differences. As a model, this new comparative interactomic approach was applied at a large scale to human papillomaviruses (HPVs) proteins. The oncogenic potential of HPVs is mainly determined by the E6 and E7 early proteins. We have mapped and overlapped the virus-host protein interaction networks of E6 and E7 proteins from 11 distinct HPV genotypes, selected for their different tropisms and pathologies. We generated robust and comprehensive datasets by combining two orthogonal protein interation assays: yeast two-hybrid (Y2H), and our recently described “High-Throughput <italic>Gaussia princeps</italic>
Protein Complementation Assay” (HT-GPCA). HT-GPCA detects protein interaction by measuring the interaction-mediated reconstitution of activity of a split <italic>Gaussia princeps</italic>
luciferase. Hierarchical clustering of interaction profiles recapitulated HPV phylogeny and was used to correlate specific virus-host interaction profiles with pathological traits, reflecting the distinct carcinogenic potentials of different HPVs. This comparative interactomics constitutes a reliable and powerful strategy to decipher molecular relationships in virtually any combination of microorganism-host interactions.</p>
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<name sortKey="Mendoza, Jose" sort="Mendoza, Jose" uniqKey="Mendoza J" first="José" last="Mendoza">José Mendoza</name>
<name sortKey="Muller, Mandy" sort="Muller, Mandy" uniqKey="Muller M" first="Mandy" last="Muller">Mandy Muller</name>
<name sortKey="Neveu, Gregory" sort="Neveu, Gregory" uniqKey="Neveu G" first="Grégory" last="Neveu">Grégory Neveu</name>
<name sortKey="Rolloy, Caroline" sort="Rolloy, Caroline" uniqKey="Rolloy C" first="Caroline" last="Rolloy">Caroline Rolloy</name>
<name sortKey="Tangy, Frederic" sort="Tangy, Frederic" uniqKey="Tangy F" first="Frédéric" last="Tangy">Frédéric Tangy</name>
<name sortKey="Vidalain, Pierre Olivier" sort="Vidalain, Pierre Olivier" uniqKey="Vidalain P" first="Pierre-Olivier" last="Vidalain">Pierre-Olivier Vidalain</name>
</noCountry>
<country name="France"><region name="Île-de-France"><name sortKey="Jones, Louis" sort="Jones, Louis" uniqKey="Jones L" first="Louis" last="Jones">Louis Jones</name>
</region>
<name sortKey="Lotteau, Vincent" sort="Lotteau, Vincent" uniqKey="Lotteau V" first="Vincent" last="Lotteau">Vincent Lotteau</name>
<name sortKey="Rabourdin Combe, Chantal" sort="Rabourdin Combe, Chantal" uniqKey="Rabourdin Combe C" first="Chantal" last="Rabourdin-Combe">Chantal Rabourdin-Combe</name>
<name sortKey="Tafforeau, Lionel" sort="Tafforeau, Lionel" uniqKey="Tafforeau L" first="Lionel" last="Tafforeau">Lionel Tafforeau</name>
<name sortKey="Trave, Gilles" sort="Trave, Gilles" uniqKey="Trave G" first="Gilles" last="Travé">Gilles Travé</name>
</country>
<country name="États-Unis"><region name="Massachusetts"><name sortKey="Dricot, Amelie" sort="Dricot, Amelie" uniqKey="Dricot A" first="Amélie" last="Dricot">Amélie Dricot</name>
</region>
<name sortKey="Hill, David E" sort="Hill, David E" uniqKey="Hill D" first="David E." last="Hill">David E. Hill</name>
<name sortKey="Vidal, Marc" sort="Vidal, Marc" uniqKey="Vidal M" first="Marc" last="Vidal">Marc Vidal</name>
</country>
</tree>
</affiliations>
</record>
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