In vivo detection of MRI-PARACEST agents in mouse brain tumors at 9.4 T.
Identifieur interne : 000458 ( PubMed/Corpus ); précédent : 000457; suivant : 000459In vivo detection of MRI-PARACEST agents in mouse brain tumors at 9.4 T.
Auteurs : Alex X. Li ; Mojmir Suchy ; Chunhui Li ; Joseph S. Gati ; Susan Meakin ; Robert H E. Hudson ; Ravi S. Menon ; Robert BarthaSource :
- Magnetic resonance in medicine [ 1522-2594 ] ; 2011.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Contrast Media.
- chemical , chemistry : Thulium.
- diagnosis : Brain Neoplasms, Glioblastoma.
- chemical , pharmacokinetics : Contrast Media, Thulium.
- Animals, Disease Models, Animal, Electron Spin Resonance Spectroscopy, Magnetic Resonance Imaging, Mice, Molecular Structure.
Abstract
Paramagnetic chemical exchange saturation transfer (PARACEST) contrast agents are under development for biological target identification by magnetic resonance imaging. Image contrast associated with PARACEST agents can be generated by radiofrequency irradiation of the chemically shifted protons bound to a PARACEST contrast agent molecule or by direct irradiation of the on-resonance bulk water protons. The observed signal change in a magnetic resonance image after the administration of a PARACEST contrast agent is due to both altered relaxation time constants and the CEST effect. Despite high sensitivity in vitro, PARACEST agents have had limited success in vivo where sensitivity is reduced by the magnetization transfer effect from endogenous macromolecules. The purpose of this study was to demonstrate the in vivo detection of a PARACEST contrast agent using the on-resonance paramagnetic chemical exchange effect (OPARACHEE) in a mouse glioblastoma multiforme tumor model and to isolate the OPARACHEE effect from the changes in relaxation induced by the PARACEST agent. Three mice with tumors were imaged on a 9.4 T MRI scanner following tail vein injection of 150 μL 50 mM Tm(3+)-DOTAM-glycine-lysine. A fast low angle shot pulse sequence with a low power radiofrequency pulse train (WALTZ-16) as the preparation pulse was used to generate OPARACHEE contrast. To study the dynamics of agent uptake, reference images (without the preparation pulse) and OPARACHEE images were acquired continuously in an alternating fashion before, during and after agent injection. Signal intensity decreased by more than 10% in tumor in the control images after agent administration. Despite these changes, a clear OPARACHEE contrast of 1-5% was also observed in brain tumors after contrast agent injection and maintained in the hour following injection. This result is the first in vivo observation of OPARACHEE contrast in brain tumors with correction of T(1) and T(2) relaxation effects.
DOI: 10.1002/mrm.22772
PubMed: 21254213
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pubmed:21254213Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">In vivo detection of MRI-PARACEST agents in mouse brain tumors at 9.4 T.</title><author><name sortKey="Li, Alex X" sort="Li, Alex X" uniqKey="Li A" first="Alex X" last="Li">Alex X. Li</name><affiliation><nlm:affiliation>Center for Functional and Metabolic Mapping, Robarts Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Suchy, Mojmir" sort="Suchy, Mojmir" uniqKey="Suchy M" first="Mojmir" last="Suchy">Mojmir Suchy</name></author><author><name sortKey="Li, Chunhui" sort="Li, Chunhui" uniqKey="Li C" first="Chunhui" last="Li">Chunhui Li</name></author><author><name sortKey="Gati, Joseph S" sort="Gati, Joseph S" uniqKey="Gati J" first="Joseph S" last="Gati">Joseph S. Gati</name></author><author><name sortKey="Meakin, Susan" sort="Meakin, Susan" uniqKey="Meakin S" first="Susan" last="Meakin">Susan Meakin</name></author><author><name sortKey="Hudson, Robert H E" sort="Hudson, Robert H E" uniqKey="Hudson R" first="Robert H E" last="Hudson">Robert H E. Hudson</name></author><author><name sortKey="Menon, Ravi S" sort="Menon, Ravi S" uniqKey="Menon R" first="Ravi S" last="Menon">Ravi S. Menon</name></author><author><name sortKey="Bartha, Robert" sort="Bartha, Robert" uniqKey="Bartha R" first="Robert" last="Bartha">Robert Bartha</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="doi">10.1002/mrm.22772</idno><idno type="RBID">pubmed:21254213</idno><idno type="pmid">21254213</idno><idno type="wicri:Area/PubMed/Corpus">000458</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000458</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">In vivo detection of MRI-PARACEST agents in mouse brain tumors at 9.4 T.</title><author><name sortKey="Li, Alex X" sort="Li, Alex X" uniqKey="Li A" first="Alex X" last="Li">Alex X. Li</name><affiliation><nlm:affiliation>Center for Functional and Metabolic Mapping, Robarts Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Suchy, Mojmir" sort="Suchy, Mojmir" uniqKey="Suchy M" first="Mojmir" last="Suchy">Mojmir Suchy</name></author><author><name sortKey="Li, Chunhui" sort="Li, Chunhui" uniqKey="Li C" first="Chunhui" last="Li">Chunhui Li</name></author><author><name sortKey="Gati, Joseph S" sort="Gati, Joseph S" uniqKey="Gati J" first="Joseph S" last="Gati">Joseph S. Gati</name></author><author><name sortKey="Meakin, Susan" sort="Meakin, Susan" uniqKey="Meakin S" first="Susan" last="Meakin">Susan Meakin</name></author><author><name sortKey="Hudson, Robert H E" sort="Hudson, Robert H E" uniqKey="Hudson R" first="Robert H E" last="Hudson">Robert H E. Hudson</name></author><author><name sortKey="Menon, Ravi S" sort="Menon, Ravi S" uniqKey="Menon R" first="Ravi S" last="Menon">Ravi S. Menon</name></author><author><name sortKey="Bartha, Robert" sort="Bartha, Robert" uniqKey="Bartha R" first="Robert" last="Bartha">Robert Bartha</name></author></analytic><series><title level="j">Magnetic resonance in medicine</title><idno type="eISSN">1522-2594</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Brain Neoplasms (diagnosis)</term><term>Contrast Media (analysis)</term><term>Contrast Media (pharmacokinetics)</term><term>Disease Models, Animal</term><term>Electron Spin Resonance Spectroscopy</term><term>Glioblastoma (diagnosis)</term><term>Magnetic Resonance Imaging</term><term>Mice</term><term>Molecular Structure</term><term>Thulium (chemistry)</term><term>Thulium (pharmacokinetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Contrast Media</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Thulium</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Brain Neoplasms</term><term>Glioblastoma</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Contrast Media</term><term>Thulium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Disease Models, Animal</term><term>Electron Spin Resonance Spectroscopy</term><term>Magnetic Resonance Imaging</term><term>Mice</term><term>Molecular Structure</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Paramagnetic chemical exchange saturation transfer (PARACEST) contrast agents are under development for biological target identification by magnetic resonance imaging. Image contrast associated with PARACEST agents can be generated by radiofrequency irradiation of the chemically shifted protons bound to a PARACEST contrast agent molecule or by direct irradiation of the on-resonance bulk water protons. The observed signal change in a magnetic resonance image after the administration of a PARACEST contrast agent is due to both altered relaxation time constants and the CEST effect. Despite high sensitivity in vitro, PARACEST agents have had limited success in vivo where sensitivity is reduced by the magnetization transfer effect from endogenous macromolecules. The purpose of this study was to demonstrate the in vivo detection of a PARACEST contrast agent using the on-resonance paramagnetic chemical exchange effect (OPARACHEE) in a mouse glioblastoma multiforme tumor model and to isolate the OPARACHEE effect from the changes in relaxation induced by the PARACEST agent. Three mice with tumors were imaged on a 9.4 T MRI scanner following tail vein injection of 150 μL 50 mM Tm(3+)-DOTAM-glycine-lysine. A fast low angle shot pulse sequence with a low power radiofrequency pulse train (WALTZ-16) as the preparation pulse was used to generate OPARACHEE contrast. To study the dynamics of agent uptake, reference images (without the preparation pulse) and OPARACHEE images were acquired continuously in an alternating fashion before, during and after agent injection. Signal intensity decreased by more than 10% in tumor in the control images after agent administration. Despite these changes, a clear OPARACHEE contrast of 1-5% was also observed in brain tumors after contrast agent injection and maintained in the hour following injection. This result is the first in vivo observation of OPARACHEE contrast in brain tumors with correction of T(1) and T(2) relaxation effects.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">21254213</PMID><DateCreated><Year>2011</Year><Month>06</Month><Day>22</Day></DateCreated><DateCompleted><Year>2011</Year><Month>12</Month><Day>19</Day></DateCompleted><DateRevised><Year>2015</Year><Month>05</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1522-2594</ISSN><JournalIssue CitedMedium="Internet"><Volume>66</Volume><Issue>1</Issue><PubDate><Year>2011</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Magnetic resonance in medicine</Title><ISOAbbreviation>Magn Reson Med</ISOAbbreviation></Journal><ArticleTitle>In vivo detection of MRI-PARACEST agents in mouse brain tumors at 9.4 T.</ArticleTitle><Pagination><MedlinePgn>67-72</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/mrm.22772</ELocationID><Abstract><AbstractText>Paramagnetic chemical exchange saturation transfer (PARACEST) contrast agents are under development for biological target identification by magnetic resonance imaging. Image contrast associated with PARACEST agents can be generated by radiofrequency irradiation of the chemically shifted protons bound to a PARACEST contrast agent molecule or by direct irradiation of the on-resonance bulk water protons. The observed signal change in a magnetic resonance image after the administration of a PARACEST contrast agent is due to both altered relaxation time constants and the CEST effect. Despite high sensitivity in vitro, PARACEST agents have had limited success in vivo where sensitivity is reduced by the magnetization transfer effect from endogenous macromolecules. The purpose of this study was to demonstrate the in vivo detection of a PARACEST contrast agent using the on-resonance paramagnetic chemical exchange effect (OPARACHEE) in a mouse glioblastoma multiforme tumor model and to isolate the OPARACHEE effect from the changes in relaxation induced by the PARACEST agent. Three mice with tumors were imaged on a 9.4 T MRI scanner following tail vein injection of 150 μL 50 mM Tm(3+)-DOTAM-glycine-lysine. A fast low angle shot pulse sequence with a low power radiofrequency pulse train (WALTZ-16) as the preparation pulse was used to generate OPARACHEE contrast. To study the dynamics of agent uptake, reference images (without the preparation pulse) and OPARACHEE images were acquired continuously in an alternating fashion before, during and after agent injection. Signal intensity decreased by more than 10% in tumor in the control images after agent administration. Despite these changes, a clear OPARACHEE contrast of 1-5% was also observed in brain tumors after contrast agent injection and maintained in the hour following injection. This result is the first in vivo observation of OPARACHEE contrast in brain tumors with correction of T(1) and T(2) relaxation effects.</AbstractText><CopyrightInformation>Copyright © 2010 Wiley-Liss, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Alex X</ForeName><Initials>AX</Initials><AffiliationInfo><Affiliation>Center for Functional and Metabolic Mapping, Robarts Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Suchy</LastName><ForeName>Mojmir</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Chunhui</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Gati</LastName><ForeName>Joseph S</ForeName><Initials>JS</Initials></Author><Author ValidYN="Y"><LastName>Meakin</LastName><ForeName>Susan</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Hudson</LastName><ForeName>Robert H E</ForeName><Initials>RH</Initials></Author><Author ValidYN="Y"><LastName>Menon</LastName><ForeName>Ravi S</ForeName><Initials>RS</Initials></Author><Author ValidYN="Y"><LastName>Bartha</LastName><ForeName>Robert</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>Canadian Institutes of Health Research</Agency><Country>Canada</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>01</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Magn Reson Med</MedlineTA><NlmUniqueID>8505245</NlmUniqueID><ISSNLinking>0740-3194</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003287">Contrast Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>8RKC5ATI4P</RegistryNumber><NameOfSubstance UI="D013932">Thulium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000818">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D001932">Brain Neoplasms</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000175">diagnosis</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y" UI="D003287">Contrast Media</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000032">analysis</QualifierName><QualifierName MajorTopicYN="N" UI="Q000493">pharmacokinetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D004195">Disease Models, Animal</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D004578">Electron Spin Resonance Spectroscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D005909">Glioblastoma</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000175">diagnosis</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y" UI="D008279">Magnetic Resonance Imaging</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D051379">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D015394">Molecular Structure</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D013932">Thulium</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName><QualifierName MajorTopicYN="N" UI="Q000493">pharmacokinetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>6</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2010</Year><Month>11</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>11</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2011</Year><Month>1</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>1</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>1</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>12</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1002/mrm.22772</ArticleId><ArticleId IdType="pubmed">21254213</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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