TmDOTA-tetraglycinate encapsulated liposomes as pH-sensitive LipoCEST agents.
Identifieur interne : 000400 ( PubMed/Corpus ); précédent : 000399; suivant : 000401TmDOTA-tetraglycinate encapsulated liposomes as pH-sensitive LipoCEST agents.
Auteurs : Ana Christina L. Opina ; Ketan B. Ghaghada ; Piyu Zhao ; Garry Kiefer ; Ananth Annapragada ; A Dean SherrySource :
- PloS one [ 1932-6203 ] ; 2011.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Amides, Coordination Complexes, Liposomes, Organometallic Compounds.
- methods : Magnetic Resonance Spectroscopy.
- Drug Compounding, Hydrodynamics, Hydrogen-Ion Concentration, Magnetics, Time Factors.
Abstract
Lanthanide DOTA-tetraglycinate (LnDOTA-(gly)₄⁻) complexes contain four magnetically equivalent amide protons that exchange with protons of bulk water. The rate of this base catalyzed exchange process has been measured using chemical exchange saturation transfer (CEST) NMR techniques as a function of solution pH for various paramagnetic LnDOTA-(gly)₄⁻ complexes to evaluate the effects of lanthanide ion size on this process. Complexes with Tb(III), Dy(III), Tm(III) and Yb(III) were chosen because these ions induce large hyperfine shifts in all ligand protons, including the exchanging amide protons. The magnitude of the amide proton CEST exchange signal differed for the four paramagnetic complexes in order, Yb>Tm>Tb>Dy. Although the Dy(III) complex showed the largest hyperfine shift as expected, the combination of favorable chemical shift and amide proton CEST linewidth in the Tm(III) complex was deemed most favorable for future in vivo applications where tissue magnetization effects can interfere. TmDOTA-(gly)₄⁻ at various concentrations was encapsulated in the core interior of liposomes to yield lipoCEST particles for molecular imaging. The resulting nanoparticles showed less than 1% leakage of the agent from the interior over a range of temperatures and pH. The pH versus amide proton CEST curves differed for the free versus encapsulated agents over the acidic pH regions, consistent with a lower proton permeability across the liposomal bilayer for the encapsulated agent. Nevertheless, the resulting lipoCEST nanoparticles amplify the CEST sensitivity by a factor of ∼10⁴ compared to the free, un-encapsulated agent. Such pH sensitive nano-probes could prove useful for pH mapping of liposomes targeted to tumors.
DOI: 10.1371/journal.pone.0027370
PubMed: 22140438
Links to Exploration step
pubmed:22140438Le document en format XML
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<front><div type="abstract" xml:lang="en">Lanthanide DOTA-tetraglycinate (LnDOTA-(gly)₄⁻) complexes contain four magnetically equivalent amide protons that exchange with protons of bulk water. The rate of this base catalyzed exchange process has been measured using chemical exchange saturation transfer (CEST) NMR techniques as a function of solution pH for various paramagnetic LnDOTA-(gly)₄⁻ complexes to evaluate the effects of lanthanide ion size on this process. Complexes with Tb(III), Dy(III), Tm(III) and Yb(III) were chosen because these ions induce large hyperfine shifts in all ligand protons, including the exchanging amide protons. The magnitude of the amide proton CEST exchange signal differed for the four paramagnetic complexes in order, Yb>Tm>Tb>Dy. Although the Dy(III) complex showed the largest hyperfine shift as expected, the combination of favorable chemical shift and amide proton CEST linewidth in the Tm(III) complex was deemed most favorable for future in vivo applications where tissue magnetization effects can interfere. TmDOTA-(gly)₄⁻ at various concentrations was encapsulated in the core interior of liposomes to yield lipoCEST particles for molecular imaging. The resulting nanoparticles showed less than 1% leakage of the agent from the interior over a range of temperatures and pH. The pH versus amide proton CEST curves differed for the free versus encapsulated agents over the acidic pH regions, consistent with a lower proton permeability across the liposomal bilayer for the encapsulated agent. Nevertheless, the resulting lipoCEST nanoparticles amplify the CEST sensitivity by a factor of ∼10⁴ compared to the free, un-encapsulated agent. Such pH sensitive nano-probes could prove useful for pH mapping of liposomes targeted to tumors.</div>
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<Abstract><AbstractText>Lanthanide DOTA-tetraglycinate (LnDOTA-(gly)₄⁻) complexes contain four magnetically equivalent amide protons that exchange with protons of bulk water. The rate of this base catalyzed exchange process has been measured using chemical exchange saturation transfer (CEST) NMR techniques as a function of solution pH for various paramagnetic LnDOTA-(gly)₄⁻ complexes to evaluate the effects of lanthanide ion size on this process. Complexes with Tb(III), Dy(III), Tm(III) and Yb(III) were chosen because these ions induce large hyperfine shifts in all ligand protons, including the exchanging amide protons. The magnitude of the amide proton CEST exchange signal differed for the four paramagnetic complexes in order, Yb>Tm>Tb>Dy. Although the Dy(III) complex showed the largest hyperfine shift as expected, the combination of favorable chemical shift and amide proton CEST linewidth in the Tm(III) complex was deemed most favorable for future in vivo applications where tissue magnetization effects can interfere. TmDOTA-(gly)₄⁻ at various concentrations was encapsulated in the core interior of liposomes to yield lipoCEST particles for molecular imaging. The resulting nanoparticles showed less than 1% leakage of the agent from the interior over a range of temperatures and pH. The pH versus amide proton CEST curves differed for the free versus encapsulated agents over the acidic pH regions, consistent with a lower proton permeability across the liposomal bilayer for the encapsulated agent. Nevertheless, the resulting lipoCEST nanoparticles amplify the CEST sensitivity by a factor of ∼10⁴ compared to the free, un-encapsulated agent. Such pH sensitive nano-probes could prove useful for pH mapping of liposomes targeted to tumors.</AbstractText>
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<CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>J Am Chem Soc. 2001 Feb 21;123(7):1517-8</RefSource>
<PMID Version="1">11456734</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Magn Reson Med. 2000 Nov;44(5):799-802</RefSource>
<PMID Version="1">11064415</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Angew Chem Int Ed Engl. 2002 Nov 15;41(22):4334-6</RefSource>
<PMID Version="1">12434381</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Invest Radiol. 2004 Apr;39(4):235-43</RefSource>
<PMID Version="1">15021328</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Biochim Biophys Acta. 1968 Jun 11;150(4):666-75</RefSource>
<PMID Version="1">5690752</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Biochem Biophys Res Commun. 1979 Aug 28;89(4):1287-93</RefSource>
<PMID Version="1">496958</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Biochem J. 1980 Feb 15;186(2):591-8</RefSource>
<PMID Version="1">7378067</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Magn Reson Med. 1989 Apr;10(1):135-44</RefSource>
<PMID Version="1">2547135</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>FEBS Lett. 1990 Jul 30;268(1):235-7</RefSource>
<PMID Version="1">2384160</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Radiology. 1991 Apr;179(1):133-7</RefSource>
<PMID Version="1">2006263</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Magn Reson Med. 1991 Feb;17(2):304-14</RefSource>
<PMID Version="1">2062205</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Biochim Biophys Acta. 1991 Aug 26;1067(2):131-8</RefSource>
<PMID Version="1">1878366</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Trends Pharmacol Sci. 1994 Jul;15(7):215-20</RefSource>
<PMID Version="1">7940982</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Angew Chem Int Ed Engl. 2005 Aug 26;44(34):5513-5</RefSource>
<PMID Version="1">16052647</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Magn Reson Med. 2006 May;55(5):1023-9</RefSource>
<PMID Version="1">16586449</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>AJNR Am J Neuroradiol. 2007 Jan;28(1):48-53</RefSource>
<PMID Version="1">17213423</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Pharm Res. 2007 Jun;24(6):1193-201</RefSource>
<PMID Version="1">17373581</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>J Am Chem Soc. 2008 Apr 16;130(15):5178-84</RefSource>
<PMID Version="1">18361490</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>J Magn Reson Imaging. 2009 Dec;30(6):1240-8</RefSource>
<PMID Version="1">19938036</PMID>
</CommentsCorrections>
<CommentsCorrections RefType="Cites"><RefSource>Magn Reson Med. 2002 Apr;47(4):639-48</RefSource>
<PMID Version="1">11948724</PMID>
</CommentsCorrections>
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