Highly shifted proton MR imaging: cell tracking by using direct detection of paramagnetic compounds.
Identifieur interne : 000176 ( PubMed/Corpus ); précédent : 000175; suivant : 000177Highly shifted proton MR imaging: cell tracking by using direct detection of paramagnetic compounds.
Auteurs : Rebecca Schmidt ; Nadine Nippe ; Klaus Strobel ; Max Masthoff ; Olga Reifschneider ; Daniela Delli Castelli ; Carsten Höltke ; Silvio Aime ; Uwe Karst ; Cord Sunderkötter ; Christoph Bremer ; Cornelius FaberSource :
- Radiology [ 1527-1315 ] ; 2014.
English descriptors
- KwdEn :
- Animals, Cell Line, Tumor, Cell Tracking (methods), Contrast Media, Female, Fibrosarcoma (pathology), Granuloma (pathology), Humans, Image Enhancement (methods), Macrophages (pathology), Magnetic Resonance Imaging (methods), Mice, Mice, Nude, Protons, Quaternary Ammonium Compounds, Reproducibility of Results, Sensitivity and Specificity.
- MESH :
- chemical : Contrast Media, Protons, Quaternary Ammonium Compounds.
- methods : Cell Tracking, Image Enhancement, Magnetic Resonance Imaging.
- pathology : Fibrosarcoma, Granuloma, Macrophages.
- Animals, Cell Line, Tumor, Female, Humans, Mice, Mice, Nude, Reproducibility of Results, Sensitivity and Specificity.
Abstract
To explore the feasibility of tracking thulium (Tm)-1,4,7,10-tetraazacyclododecane-α,α',α'',α'''-tetramethyl-1,4,7,10-tetraacetic acid (DOTMA)-labeled cells in vivo by means of highly shifted proton magnetic resonance (MR) imaging as a potential alternative to established cell-tracking methods.
DOI: 10.1148/radiol.14132056
PubMed: 24852443
Links to Exploration step
pubmed:24852443Le document en format XML
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sortKey="Nippe, Nadine" sort="Nippe, Nadine" uniqKey="Nippe N" first="Nadine" last="Nippe">Nadine Nippe</name></author><author><name sortKey="Strobel, Klaus" sort="Strobel, Klaus" uniqKey="Strobel K" first="Klaus" last="Strobel">Klaus Strobel</name></author><author><name sortKey="Masthoff, Max" sort="Masthoff, Max" uniqKey="Masthoff M" first="Max" last="Masthoff">Max Masthoff</name></author><author><name sortKey="Reifschneider, Olga" sort="Reifschneider, Olga" uniqKey="Reifschneider O" first="Olga" last="Reifschneider">Olga Reifschneider</name></author><author><name sortKey="Castelli, Daniela Delli" sort="Castelli, Daniela Delli" uniqKey="Castelli D" first="Daniela Delli" last="Castelli">Daniela Delli Castelli</name></author><author><name sortKey="Holtke, Carsten" sort="Holtke, Carsten" uniqKey="Holtke C" first="Carsten" last="Höltke">Carsten Höltke</name></author><author><name sortKey="Aime, Silvio" sort="Aime, Silvio" uniqKey="Aime S" first="Silvio" last="Aime">Silvio Aime</name></author><author><name sortKey="Karst, Uwe" sort="Karst, Uwe" uniqKey="Karst U" first="Uwe" last="Karst">Uwe Karst</name></author><author><name sortKey="Sunderkotter, Cord" sort="Sunderkotter, Cord" uniqKey="Sunderkotter C" first="Cord" last="Sunderkötter">Cord Sunderkötter</name></author><author><name sortKey="Bremer, Christoph" sort="Bremer, Christoph" uniqKey="Bremer C" first="Christoph" last="Bremer">Christoph Bremer</name></author><author><name sortKey="Faber, Cornelius" sort="Faber, Cornelius" uniqKey="Faber C" first="Cornelius" last="Faber">Cornelius Faber</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="doi">10.1148/radiol.14132056</idno><idno type="RBID">pubmed:24852443</idno><idno type="pmid">24852443</idno><idno type="wicri:Area/PubMed/Corpus">000176</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000176</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Highly shifted proton MR imaging: cell tracking by using direct detection of paramagnetic compounds.</title><author><name sortKey="Schmidt, Rebecca" sort="Schmidt, Rebecca" uniqKey="Schmidt R" first="Rebecca" last="Schmidt">Rebecca Schmidt</name><affiliation><nlm:affiliation>From the Department of Clinical Radiology (R.S., K.S., M.M., C.H., U.K., C.B., C.F.) and Clinic of Dermatology-General Dermatology and Venerology (N.N., C.S.), University Hospital Münster, Albert-Schweitzer-Campus 1, 48149 Münster, Germany; Institute of Inorganic and Analytical Chemistry, University of Münster, Münster, Germany (O.R., U.K.); Department of Molecular Biotechnologies and Health Sciences, University of Torino, Turin, Italy (D.D.C., S.A.); Cluster of Excellence EXC 1003, Cells in Motion, Münster, Germany (U.K., C.S., C.B., C.F.); and Bruker Biospin GmbH, Ettlingen, Germany (K.S.).</nlm:affiliation></affiliation></author><author><name sortKey="Nippe, Nadine" sort="Nippe, Nadine" uniqKey="Nippe N" first="Nadine" last="Nippe">Nadine Nippe</name></author><author><name sortKey="Strobel, Klaus" sort="Strobel, Klaus" uniqKey="Strobel K" first="Klaus" last="Strobel">Klaus Strobel</name></author><author><name sortKey="Masthoff, Max" sort="Masthoff, Max" uniqKey="Masthoff M" first="Max" last="Masthoff">Max Masthoff</name></author><author><name sortKey="Reifschneider, Olga" sort="Reifschneider, Olga" uniqKey="Reifschneider O" first="Olga" last="Reifschneider">Olga Reifschneider</name></author><author><name sortKey="Castelli, Daniela Delli" sort="Castelli, Daniela Delli" uniqKey="Castelli D" first="Daniela Delli" last="Castelli">Daniela Delli Castelli</name></author><author><name sortKey="Holtke, Carsten" sort="Holtke, Carsten" uniqKey="Holtke C" first="Carsten" last="Höltke">Carsten Höltke</name></author><author><name sortKey="Aime, Silvio" sort="Aime, Silvio" uniqKey="Aime S" first="Silvio" last="Aime">Silvio Aime</name></author><author><name sortKey="Karst, Uwe" sort="Karst, Uwe" uniqKey="Karst U" first="Uwe" last="Karst">Uwe Karst</name></author><author><name sortKey="Sunderkotter, Cord" sort="Sunderkotter, Cord" uniqKey="Sunderkotter C" first="Cord" last="Sunderkötter">Cord Sunderkötter</name></author><author><name sortKey="Bremer, Christoph" sort="Bremer, Christoph" uniqKey="Bremer C" first="Christoph" last="Bremer">Christoph Bremer</name></author><author><name sortKey="Faber, Cornelius" sort="Faber, Cornelius" uniqKey="Faber C" first="Cornelius" last="Faber">Cornelius Faber</name></author></analytic><series><title level="j">Radiology</title><idno type="eISSN">1527-1315</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Line, Tumor</term><term>Cell Tracking (methods)</term><term>Contrast Media</term><term>Female</term><term>Fibrosarcoma (pathology)</term><term>Granuloma (pathology)</term><term>Humans</term><term>Image Enhancement (methods)</term><term>Macrophages (pathology)</term><term>Magnetic Resonance Imaging (methods)</term><term>Mice</term><term>Mice, Nude</term><term>Protons</term><term>Quaternary Ammonium Compounds</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Contrast Media</term><term>Protons</term><term>Quaternary Ammonium Compounds</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cell Tracking</term><term>Image Enhancement</term><term>Magnetic Resonance Imaging</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Fibrosarcoma</term><term>Granuloma</term><term>Macrophages</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line, Tumor</term><term>Female</term><term>Humans</term><term>Mice</term><term>Mice, Nude</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To explore the feasibility of tracking thulium (Tm)-1,4,7,10-tetraazacyclododecane-α,α',α'',α'''-tetramethyl-1,4,7,10-tetraacetic acid (DOTMA)-labeled cells in vivo by means of highly shifted proton magnetic resonance (MR) imaging as a potential alternative to established cell-tracking methods.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">24852443</PMID><DateCreated><Year>2014</Year><Month>08</Month><Day>26</Day></DateCreated><DateCompleted><Year>2014</Year><Month>10</Month><Day>15</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1527-1315</ISSN><JournalIssue CitedMedium="Internet"><Volume>272</Volume><Issue>3</Issue><PubDate><Year>2014</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Radiology</Title><ISOAbbreviation>Radiology</ISOAbbreviation></Journal><ArticleTitle>Highly shifted proton MR imaging: cell tracking by using direct detection of paramagnetic compounds.</ArticleTitle><Pagination><MedlinePgn>785-95</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1148/radiol.14132056</ELocationID><Abstract><AbstractText Label="PURPOSE" NlmCategory="OBJECTIVE">To explore the feasibility of tracking thulium (Tm)-1,4,7,10-tetraazacyclododecane-α,α',α'',α'''-tetramethyl-1,4,7,10-tetraacetic acid (DOTMA)-labeled cells in vivo by means of highly shifted proton magnetic resonance (MR) imaging as a potential alternative to established cell-tracking methods.</AbstractText><AbstractText Label="MATERIALS AND METHODS" NlmCategory="METHODS">All animal experiments were approved by the local ethics committee for animal experiments. Highly shifted proton MR imaging is based on the principle that the shifted resonances on Tm and dysprosium (Dy)-DOTMA can be detected separately from the tissue water signal at MR imaging with very short echo time and radial center-out readout (UTE, or "ultrashort echo time"). MR imaging of aqueous solutions and in mice in vivo was performed at 9.4 T. Human fibrosarcoma cells (HT-1080) and murine macrophages were labeled with different amounts of Tm-DOTMA. Labeled fibrosarcoma cells were injected subcutaneously into three mice. For cell tracking, labeled macrophages were administered intravenously into eight mice bearing local granulomatous inflammation. Three-dimensional UTE MR imaging was performed during 1 week. Macrophage viability and activity and fibrosarcoma cell viability were statistically analyzed by performing an unpaired two-tailed t test for labeled versus unlabeled cells by using data of at least six independent experiments.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The strongly shifted MR lines of Tm- and Dy-DOTMA can be separated from the tissue water signal and from each other. A detection limit of about 25 µmol/L of Tm-DOTMA was calculated from in vitro MR measurements. A mean ± standard error of the mean intracellular uptake of (4.19 ± 0.88) × 10(9) (HT-1080) and (10.1 ± 3.0) × 10(10) (macrophages) of Tm-DOTMA molecules per cell was achieved. In vivo, Tm-DOTMA signal was detectable for 1 week in both tumors and macrophages, with a detection limit of approximately 10(4) HT-1080 and 600 macrophages. Histologic examination results and elemental bioimaging confirmed labeled cells as source of MR signal.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Strongly shifted proton three-dimensional UTE MR imaging of Tm-DOTMA-labeled cells is a highly specific and sensitive tool for in vivo cell tracking.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Schmidt</LastName><ForeName>Rebecca</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>From the Department of Clinical Radiology (R.S., K.S., M.M., C.H., U.K., C.B., C.F.) and Clinic of Dermatology-General Dermatology and Venerology (N.N., C.S.), University Hospital Münster, Albert-Schweitzer-Campus 1, 48149 Münster, Germany; Institute of Inorganic and Analytical Chemistry, University of Münster, Münster, Germany (O.R., U.K.); Department of Molecular Biotechnologies and Health Sciences, University of Torino, Turin, Italy (D.D.C., S.A.); Cluster of Excellence EXC 1003, Cells in Motion, Münster, Germany (U.K., C.S., C.B., C.F.); and Bruker Biospin GmbH, Ettlingen, Germany (K.S.).</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nippe</LastName><ForeName>Nadine</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Strobel</LastName><ForeName>Klaus</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Masthoff</LastName><ForeName>Max</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Reifschneider</LastName><ForeName>Olga</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Castelli</LastName><ForeName>Daniela Delli</ForeName><Initials>DD</Initials></Author><Author ValidYN="Y"><LastName>Höltke</LastName><ForeName>Carsten</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Aime</LastName><ForeName>Silvio</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Karst</LastName><ForeName>Uwe</ForeName><Initials>U</Initials></Author><Author ValidYN="Y"><LastName>Sunderkötter</LastName><ForeName>Cord</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Bremer</LastName><ForeName>Christoph</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Faber</LastName><ForeName>Cornelius</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>05</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Radiology</MedlineTA><NlmUniqueID>0401260</NlmUniqueID><ISSNLinking>0033-8419</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003287">Contrast Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011522">Protons</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000644">Quaternary Ammonium Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>104162-48-3</RegistryNumber><NameOfSubstance UI="C054005">N-(1-(2,3-dioleyloxy)propyl)-N,N,N-trimethylammonium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>AIM</CitationSubset><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="CommentIn"><RefSource>Radiology. 2014 Sep;272(3):615-7</RefSource><PMID Version="1">25153271</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000818">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D045744">Cell Line, Tumor</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D058948">Cell Tracking</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000379">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D003287">Contrast Media</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D005260">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D005354">Fibrosarcoma</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000473">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D006099">Granuloma</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000473">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D006801">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D007089">Image Enhancement</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000379">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D008264">Macrophages</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000473">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D008279">Magnetic Resonance Imaging</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000379">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D051379">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D008819">Mice, Nude</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D011522">Protons</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y" UI="D000644">Quaternary Ammonium Compounds</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D015203">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D012680">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="aheadofprint"><Year>2014</Year><Month>5</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>5</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>5</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>10</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1148/radiol.14132056</ArticleId><ArticleId IdType="pubmed">24852443</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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