Calcium imaging of infrared-stimulated activity in rodent brain
Identifieur interne : 000327 ( Pmc/Corpus ); précédent : 000326; suivant : 000328Calcium imaging of infrared-stimulated activity in rodent brain
Auteurs : Jonathan Matthew Cayce ; Matthew B. Bouchard ; Mykyta M. Chernov ; Brenda R. Chen ; Lauren E. Grosberg ; E. Duco Jansen ; Elizabeth M. C. Hillman ; Anita Mahadevan-JansenSource :
- Cell calcium [ 0143-4160 ] ; 2014.
Abstract
Infrared neural stimulation (INS) is a promising neurostimulation technique that can activate neural tissue with high spatial precision and without the need for exogenous agents. However, little is understood about how infrared light interacts with neural tissue on a cellular level, particularly within the living brain. In this study, we use calcium sensitive dye imaging on macroscopic and microscopic scales to explore the spatiotemporal effects of INS on cortical calcium dynamics. The INS-evoked calcium signal that was observed exhibited a fast and slow component suggesting activation of multiple cellular mechanisms. The slow component of the evoked signal exhibited wave-like properties suggesting network activation, and was verified to originate from astrocytes through pharmacology and 2-photon imaging. We also provide evidence that the fast calcium signal may have been evoked through modulation of glutamate transients. This study demonstrates that pulsed infrared light can induce intracellular calcium modulations in both astrocytes and neurons, providing new insights into the mechanisms of action of INS in the brain.
Url:
DOI: 10.1016/j.ceca.2014.01.004
PubMed: 24674600
PubMed Central: 4014070
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Calcium imaging of infrared-stimulated activity in rodent brain</title><author><name sortKey="Cayce, Jonathan Matthew" sort="Cayce, Jonathan Matthew" uniqKey="Cayce J" first="Jonathan Matthew" last="Cayce">Jonathan Matthew Cayce</name><affiliation><nlm:aff id="A1">Department of Biomedical Engineering, Vanderbilt University, Station B, Box 351631, Nashville, TN 37235</nlm:aff></affiliation></author><author><name sortKey="Bouchard, Matthew B" sort="Bouchard, Matthew B" uniqKey="Bouchard M" first="Matthew B." last="Bouchard">Matthew B. Bouchard</name><affiliation><nlm:aff id="A2">Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Columbia University, 120th Street and Amsterdam Ave New York, NY 10027</nlm:aff></affiliation></author><author><name sortKey="Chernov, Mykyta M" sort="Chernov, Mykyta M" uniqKey="Chernov M" first="Mykyta M." last="Chernov">Mykyta M. Chernov</name><affiliation><nlm:aff id="A1">Department of Biomedical Engineering, Vanderbilt University, Station B, Box 351631, Nashville, TN 37235</nlm:aff></affiliation></author><author><name sortKey="Chen, Brenda R" sort="Chen, Brenda R" uniqKey="Chen B" first="Brenda R." last="Chen">Brenda R. Chen</name><affiliation><nlm:aff id="A2">Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Columbia University, 120th Street and Amsterdam Ave New York, NY 10027</nlm:aff></affiliation></author><author><name sortKey="Grosberg, Lauren E" sort="Grosberg, Lauren E" uniqKey="Grosberg L" first="Lauren E." last="Grosberg">Lauren E. Grosberg</name><affiliation><nlm:aff id="A2">Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Columbia University, 120th Street and Amsterdam Ave New York, NY 10027</nlm:aff></affiliation></author><author><name sortKey="Jansen, E Duco" sort="Jansen, E Duco" uniqKey="Jansen E" first="E. Duco" last="Jansen">E. Duco Jansen</name><affiliation><nlm:aff id="A1">Department of Biomedical Engineering, Vanderbilt University, Station B, Box 351631, Nashville, TN 37235</nlm:aff></affiliation></author><author><name sortKey="Hillman, Elizabeth M C" sort="Hillman, Elizabeth M C" uniqKey="Hillman E" first="Elizabeth M. C." last="Hillman">Elizabeth M. C. Hillman</name><affiliation><nlm:aff id="A2">Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Columbia University, 120th Street and Amsterdam Ave New York, NY 10027</nlm:aff></affiliation></author><author><name sortKey="Mahadevan Jansen, Anita" sort="Mahadevan Jansen, Anita" uniqKey="Mahadevan Jansen A" first="Anita" last="Mahadevan-Jansen">Anita Mahadevan-Jansen</name><affiliation><nlm:aff id="A1">Department of Biomedical Engineering, Vanderbilt University, Station B, Box 351631, Nashville, TN 37235</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24674600</idno><idno type="pmc">4014070</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014070</idno><idno type="RBID">PMC:4014070</idno><idno type="doi">10.1016/j.ceca.2014.01.004</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000327</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000327</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Calcium imaging of infrared-stimulated activity in rodent brain</title><author><name sortKey="Cayce, Jonathan Matthew" sort="Cayce, Jonathan Matthew" uniqKey="Cayce J" first="Jonathan Matthew" last="Cayce">Jonathan Matthew Cayce</name><affiliation><nlm:aff id="A1">Department of Biomedical Engineering, Vanderbilt University, Station B, Box 351631, Nashville, TN 37235</nlm:aff></affiliation></author><author><name sortKey="Bouchard, Matthew B" sort="Bouchard, Matthew B" uniqKey="Bouchard M" first="Matthew B." last="Bouchard">Matthew B. Bouchard</name><affiliation><nlm:aff id="A2">Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Columbia University, 120th Street and Amsterdam Ave New York, NY 10027</nlm:aff></affiliation></author><author><name sortKey="Chernov, Mykyta M" sort="Chernov, Mykyta M" uniqKey="Chernov M" first="Mykyta M." last="Chernov">Mykyta M. Chernov</name><affiliation><nlm:aff id="A1">Department of Biomedical Engineering, Vanderbilt University, Station B, Box 351631, Nashville, TN 37235</nlm:aff></affiliation></author><author><name sortKey="Chen, Brenda R" sort="Chen, Brenda R" uniqKey="Chen B" first="Brenda R." last="Chen">Brenda R. Chen</name><affiliation><nlm:aff id="A2">Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Columbia University, 120th Street and Amsterdam Ave New York, NY 10027</nlm:aff></affiliation></author><author><name sortKey="Grosberg, Lauren E" sort="Grosberg, Lauren E" uniqKey="Grosberg L" first="Lauren E." last="Grosberg">Lauren E. Grosberg</name><affiliation><nlm:aff id="A2">Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Columbia University, 120th Street and Amsterdam Ave New York, NY 10027</nlm:aff></affiliation></author><author><name sortKey="Jansen, E Duco" sort="Jansen, E Duco" uniqKey="Jansen E" first="E. Duco" last="Jansen">E. Duco Jansen</name><affiliation><nlm:aff id="A1">Department of Biomedical Engineering, Vanderbilt University, Station B, Box 351631, Nashville, TN 37235</nlm:aff></affiliation></author><author><name sortKey="Hillman, Elizabeth M C" sort="Hillman, Elizabeth M C" uniqKey="Hillman E" first="Elizabeth M. C." last="Hillman">Elizabeth M. C. Hillman</name><affiliation><nlm:aff id="A2">Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Columbia University, 120th Street and Amsterdam Ave New York, NY 10027</nlm:aff></affiliation></author><author><name sortKey="Mahadevan Jansen, Anita" sort="Mahadevan Jansen, Anita" uniqKey="Mahadevan Jansen A" first="Anita" last="Mahadevan-Jansen">Anita Mahadevan-Jansen</name><affiliation><nlm:aff id="A1">Department of Biomedical Engineering, Vanderbilt University, Station B, Box 351631, Nashville, TN 37235</nlm:aff></affiliation></author></analytic><series><title level="j">Cell calcium</title><idno type="ISSN">0143-4160</idno><idno type="eISSN">1532-1991</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Summary</title><p id="P3">Infrared neural stimulation (INS) is a promising neurostimulation technique that can activate neural tissue with high spatial precision and without the need for exogenous agents. However, little is understood about how infrared light interacts with neural tissue on a cellular level, particularly within the living brain. In this study, we use calcium sensitive dye imaging on macroscopic and microscopic scales to explore the spatiotemporal effects of INS on cortical calcium dynamics. The INS-evoked calcium signal that was observed exhibited a fast and slow component suggesting activation of multiple cellular mechanisms. The slow component of the evoked signal exhibited wave-like properties suggesting network activation, and was verified to originate from astrocytes through pharmacology and 2-photon imaging. We also provide evidence that the fast calcium signal may have been evoked through modulation of glutamate transients. This study demonstrates that pulsed infrared light can induce intracellular calcium modulations in both astrocytes and neurons, providing new insights into the mechanisms of action of INS in the brain.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">8006226</journal-id><journal-id journal-id-type="pubmed-jr-id">2840</journal-id><journal-id journal-id-type="nlm-ta">Cell Calcium</journal-id><journal-id journal-id-type="iso-abbrev">Cell Calcium</journal-id><journal-title-group><journal-title>Cell calcium</journal-title></journal-title-group><issn pub-type="ppub">0143-4160</issn><issn pub-type="epub">1532-1991</issn></journal-meta><article-meta><article-id pub-id-type="pmid">24674600</article-id><article-id pub-id-type="pmc">4014070</article-id><article-id pub-id-type="doi">10.1016/j.ceca.2014.01.004</article-id><article-id pub-id-type="manuscript">NIHMS562589</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Calcium imaging of infrared-stimulated activity in rodent brain</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Cayce</surname><given-names>Jonathan Matthew</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Bouchard</surname><given-names>Matthew B.</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Chernov</surname><given-names>Mykyta M.</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Brenda R.</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Grosberg</surname><given-names>Lauren E.</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Jansen</surname><given-names>E. Duco</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Hillman</surname><given-names>Elizabeth M. C.</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Mahadevan-Jansen</surname><given-names>Anita</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref rid="FN1" ref-type="author-notes">*</xref></contrib></contrib-group><aff id="A1"><label>1</label>Department of Biomedical Engineering, Vanderbilt University, Station B, Box 351631, Nashville, TN 37235</aff><aff id="A2"><label>2</label>Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Columbia University, 120th Street and Amsterdam Ave New York, NY 10027</aff><author-notes><corresp id="FN1">Corresponding Author: Dr. Anita Mahadevan-Jansen, Station B, Box 351631, Nashville, TN 37235, <email>anita.mahadevan-jansen@vanderbilt.edu</email>, 615-343-4787</corresp></author-notes><pub-date pub-type="nihms-submitted"><day>25</day><month>4</month><year>2014</year></pub-date><pub-date pub-type="epub"><day>31</day><month>1</month><year>2014</year></pub-date><pub-date pub-type="ppub"><month>4</month><year>2014</year></pub-date><pub-date pub-type="pmc-release"><day>01</day><month>4</month><year>2015</year></pub-date><volume>55</volume><issue>4</issue><fpage>183</fpage><lpage>190</lpage><pmc-comment>elocation-id from pubmed: 10.1016/j.ceca.2014.01.004</pmc-comment>
<permissions><copyright-statement>© 2014 Elsevier Ltd. All rights reserved.</copyright-statement><copyright-year>2014</copyright-year></permissions><abstract><title>Summary</title><p id="P3">Infrared neural stimulation (INS) is a promising neurostimulation technique that can activate neural tissue with high spatial precision and without the need for exogenous agents. However, little is understood about how infrared light interacts with neural tissue on a cellular level, particularly within the living brain. In this study, we use calcium sensitive dye imaging on macroscopic and microscopic scales to explore the spatiotemporal effects of INS on cortical calcium dynamics. The INS-evoked calcium signal that was observed exhibited a fast and slow component suggesting activation of multiple cellular mechanisms. The slow component of the evoked signal exhibited wave-like properties suggesting network activation, and was verified to originate from astrocytes through pharmacology and 2-photon imaging. We also provide evidence that the fast calcium signal may have been evoked through modulation of glutamate transients. This study demonstrates that pulsed infrared light can induce intracellular calcium modulations in both astrocytes and neurons, providing new insights into the mechanisms of action of INS in the brain.</p></abstract><kwd-group><kwd>infrared neural stimulation</kwd><kwd>calcium signaling</kwd><kwd>somatosensory cortex</kwd><kwd>optical imaging</kwd><kwd>calcium dye imaging</kwd><kwd>neurons</kwd><kwd>astrocytes</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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