Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">NIR to NIR upconversion in KYb2F7: RE<sup>3+</sup> (RE = Tm, Er) nanoparticles for biological imaging</title><author><name sortKey="Pedraza, F" sort="Pedraza, F" uniqKey="Pedraza F" first="F." last="Pedraza">F. Pedraza</name><affiliation><nlm:aff id="A1">University of Texas at San Antonio, Physics and Astronomy Department</nlm:aff></affiliation></author><author><name sortKey="Yust, B" sort="Yust, B" uniqKey="Yust B" first="B." last="Yust">B. Yust</name><affiliation><nlm:aff id="A1">University of Texas at San Antonio, Physics and Astronomy Department</nlm:aff></affiliation></author><author><name sortKey="Tsin, A" sort="Tsin, A" uniqKey="Tsin A" first="A." last="Tsin">A. Tsin</name><affiliation><nlm:aff id="A2">University of Texas at San Antonio, Department of Biology</nlm:aff></affiliation></author><author><name sortKey="Sardar, D" sort="Sardar, D" uniqKey="Sardar D" first="D." last="Sardar">D. Sardar</name><affiliation><nlm:aff id="A1">University of Texas at San Antonio, Physics and Astronomy Department</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26120238</idno><idno type="pmc">4482478</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4482478</idno><idno type="RBID">PMC:4482478</idno><idno type="doi">10.1117/12.2037913</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000316</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000316</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">NIR to NIR upconversion in KYb2F7: RE<sup>3+</sup> (RE = Tm, Er) nanoparticles for biological imaging</title><author><name sortKey="Pedraza, F" sort="Pedraza, F" uniqKey="Pedraza F" first="F." last="Pedraza">F. Pedraza</name><affiliation><nlm:aff id="A1">University of Texas at San Antonio, Physics and Astronomy Department</nlm:aff></affiliation></author><author><name sortKey="Yust, B" sort="Yust, B" uniqKey="Yust B" first="B." last="Yust">B. Yust</name><affiliation><nlm:aff id="A1">University of Texas at San Antonio, Physics and Astronomy Department</nlm:aff></affiliation></author><author><name sortKey="Tsin, A" sort="Tsin, A" uniqKey="Tsin A" first="A." last="Tsin">A. Tsin</name><affiliation><nlm:aff id="A2">University of Texas at San Antonio, Department of Biology</nlm:aff></affiliation></author><author><name sortKey="Sardar, D" sort="Sardar, D" uniqKey="Sardar D" first="D." last="Sardar">D. Sardar</name><affiliation><nlm:aff id="A1">University of Texas at San Antonio, Physics and Astronomy Department</nlm:aff></affiliation></author></analytic><series><title level="j">Proceedings of SPIE--the International Society for Optical Engineering</title><idno type="ISSN">0277-786X</idno><idno type="eISSN">1996-756X</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Until recently, many contrast agents widely used in biological imaging have absorbed and emitted in the visible region, limiting their usefulness for deeper tissue imaging. In order to push the boundaries of deep tissue imaging with non-ionizing radiation, contrast agents in the near infrared (NIR) regime, which is not strongly absorbed or scattered by most tissues, are being sought after. Upconverting nanoparticles (UCNPs) are attractive candidates since their upconversion emission is tunable with a very narrow bandwidth and they do not photobleach or blink. The upconversion produced by the nanoparticles can be tailored for NIR to NIR by carefully choosing the lanthanide dopants and dopant ratios such as KYb<sub>2</sub>F<sub>7</sub>: RE<sup>3+</sup> (RE = Tm, Er). Spectroscopic characterization was done by analyzing absorption, fluorescence, and quantum yield data. In order to study the toxicity of the nanoparticles Monkey Retinal Endothelial Cells (MREC) were cultivated in 24 well plates and then treated with nanoparticles at different concentrations in triplicate to obtain the optimal concentration for in vivo experiments. It will be shown that these UCNPs do not elicit a strong toxic response such as quantum dots and some noble metal nanoparticles. 3-D optical slices of nanoparticle treated fibroblast cells were imaged using a confocal microscope where the nucleus and cytoplasm were stained with DAPI and Alexa Fluor respectively. These results presented support the initial assumption, which suggests that KYb<sub>2</sub>F<sub>7</sub>: RE<sup>3+</sup> would be excellent candidates for NIR contrast agents.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">101524122</journal-id><journal-id journal-id-type="pubmed-jr-id">37467</journal-id><journal-id journal-id-type="nlm-ta">Proc SPIE Int Soc Opt Eng</journal-id><journal-id journal-id-type="iso-abbrev">Proc SPIE Int Soc Opt Eng</journal-id><journal-title-group><journal-title>Proceedings of SPIE--the International Society for Optical Engineering</journal-title></journal-title-group><issn pub-type="ppub">0277-786X</issn><issn pub-type="epub">1996-756X</issn></journal-meta><article-meta><article-id pub-id-type="pmid">26120238</article-id><article-id pub-id-type="pmc">4482478</article-id><article-id pub-id-type="doi">10.1117/12.2037913</article-id><article-id pub-id-type="manuscript">NIHMS701476</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>NIR to NIR upconversion in KYb2F7: RE<sup>3+</sup> (RE = Tm, Er) nanoparticles for biological imaging</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Pedraza</surname><given-names>F.</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Yust</surname><given-names>B.</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Tsin</surname><given-names>A.</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Sardar</surname><given-names>D.</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib></contrib-group><aff id="A1"><label>1</label>University of Texas at San Antonio, Physics and Astronomy Department</aff><aff id="A2"><label>2</label>University of Texas at San Antonio, Department of Biology</aff><pub-date pub-type="nihms-submitted"><day>20</day><month>6</month><year>2015</year></pub-date><pub-date pub-type="epub"><day>7</day><month>3</month><year>2014</year></pub-date><pub-date pub-type="ppub"><day>1</day><month>2</month><year>2014</year></pub-date><pub-date pub-type="pmc-release"><day>26</day><month>6</month><year>2015</year></pub-date><volume>8956</volume><elocation-id>89560Q</elocation-id><permissions><copyright-statement>© 2014 SPIE</copyright-statement><copyright-year>2014</copyright-year></permissions><abstract><p id="P1">Until recently, many contrast agents widely used in biological imaging have absorbed and emitted in the visible region, limiting their usefulness for deeper tissue imaging. In order to push the boundaries of deep tissue imaging with non-ionizing radiation, contrast agents in the near infrared (NIR) regime, which is not strongly absorbed or scattered by most tissues, are being sought after. Upconverting nanoparticles (UCNPs) are attractive candidates since their upconversion emission is tunable with a very narrow bandwidth and they do not photobleach or blink. The upconversion produced by the nanoparticles can be tailored for NIR to NIR by carefully choosing the lanthanide dopants and dopant ratios such as KYb<sub>2</sub>F<sub>7</sub>: RE<sup>3+</sup> (RE = Tm, Er). Spectroscopic characterization was done by analyzing absorption, fluorescence, and quantum yield data. In order to study the toxicity of the nanoparticles Monkey Retinal Endothelial Cells (MREC) were cultivated in 24 well plates and then treated with nanoparticles at different concentrations in triplicate to obtain the optimal concentration for in vivo experiments. It will be shown that these UCNPs do not elicit a strong toxic response such as quantum dots and some noble metal nanoparticles. 3-D optical slices of nanoparticle treated fibroblast cells were imaged using a confocal microscope where the nucleus and cytoplasm were stained with DAPI and Alexa Fluor respectively. These results presented support the initial assumption, which suggests that KYb<sub>2</sub>F<sub>7</sub>: RE<sup>3+</sup> would be excellent candidates for NIR contrast agents.</p></abstract><kwd-group><kwd>Upconverting nanoparticles</kwd><kwd>biomedical imaging</kwd><kwd>rare-earth nanoparticles</kwd><kwd>near infrared</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/ThuliumV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000316 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000316 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Terre
|area= ThuliumV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé=
|texte=
}}
| This area was generated with Dilib version V0.6.21. |  |