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Element-tagged immunoassay with ICP-MS detection: evaluation and comparison to conventional immunoassays

Identifieur interne : 000219 ( Pmc/Corpus ); précédent : 000218; suivant : 000220

Element-tagged immunoassay with ICP-MS detection: evaluation and comparison to conventional immunoassays

Auteurs : Eva Razumienko ; Olga Ornatsky ; Robert Kinach ; Michael Milyavsky ; Eric Lechman ; Vladimir Baranov ; Mitchell A. Winnik ; Scott D. Tanner

Source :

Journal of immunological methods [ 0022-1759 ] ; 2008.

RBID : PMC:2583136

Abstract

We have investigated the possibility of using element-tagged antibodies for protein detection and quantification in microplate format using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), and compared the results to conventional immunoassays, such as Enzyme-Linked Immunosorbent Assay (ELISA) and Western blotting. The technique was further employed to detect low levels and measure DNA-binding activity of transcription factor p53 in leukemia cell lysates through its interaction with immobilized oligonucleotides and recognition by element-tagged antibodies. The advantages of ICP-MS detection for routine performance of immunoassays include increased sensitivity, wide dynamic range, minimal interference from complex matrices, and high throughput. Our approach advances the ICP-MS technology and demonstrates its applicability to proteomic studies through the use of antibodies directly labeled with polymer tags bearing multiple atoms of lanthanides. Development of this novel methodology will enable fast and quantitative identification of multiple analytes in a single well.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2583136

DOI: 10.1016/j.jim.2008.03.011
PubMed: 18456275
PubMed Central: 2583136

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PMC:2583136

Le document en format XML

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<author><name sortKey="Razumienko, Eva" sort="Razumienko, Eva" uniqKey="Razumienko E" first="Eva" last="Razumienko">Eva Razumienko</name>
<affiliation><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
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<author><name sortKey="Ornatsky, Olga" sort="Ornatsky, Olga" uniqKey="Ornatsky O" first="Olga" last="Ornatsky">Olga Ornatsky</name>
<affiliation><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
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<author><name sortKey="Kinach, Robert" sort="Kinach, Robert" uniqKey="Kinach R" first="Robert" last="Kinach">Robert Kinach</name>
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<author><name sortKey="Milyavsky, Michael" sort="Milyavsky, Michael" uniqKey="Milyavsky M" first="Michael" last="Milyavsky">Michael Milyavsky</name>
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<author><name sortKey="Lechman, Eric" sort="Lechman, Eric" uniqKey="Lechman E" first="Eric" last="Lechman">Eric Lechman</name>
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<author><name sortKey="Baranov, Vladimir" sort="Baranov, Vladimir" uniqKey="Baranov V" first="Vladimir" last="Baranov">Vladimir Baranov</name>
<affiliation><nlm:aff id="A2">Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada.</nlm:aff>
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<author><name sortKey="Winnik, Mitchell A" sort="Winnik, Mitchell A" uniqKey="Winnik M" first="Mitchell A." last="Winnik">Mitchell A. Winnik</name>
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<author><name sortKey="Tanner, Scott D" sort="Tanner, Scott D" uniqKey="Tanner S" first="Scott D." last="Tanner">Scott D. Tanner</name>
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<author><name sortKey="Ornatsky, Olga" sort="Ornatsky, Olga" uniqKey="Ornatsky O" first="Olga" last="Ornatsky">Olga Ornatsky</name>
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<author><name sortKey="Kinach, Robert" sort="Kinach, Robert" uniqKey="Kinach R" first="Robert" last="Kinach">Robert Kinach</name>
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<author><name sortKey="Milyavsky, Michael" sort="Milyavsky, Michael" uniqKey="Milyavsky M" first="Michael" last="Milyavsky">Michael Milyavsky</name>
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<author><name sortKey="Lechman, Eric" sort="Lechman, Eric" uniqKey="Lechman E" first="Eric" last="Lechman">Eric Lechman</name>
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<author><name sortKey="Baranov, Vladimir" sort="Baranov, Vladimir" uniqKey="Baranov V" first="Vladimir" last="Baranov">Vladimir Baranov</name>
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<author><name sortKey="Winnik, Mitchell A" sort="Winnik, Mitchell A" uniqKey="Winnik M" first="Mitchell A." last="Winnik">Mitchell A. Winnik</name>
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<author><name sortKey="Tanner, Scott D" sort="Tanner, Scott D" uniqKey="Tanner S" first="Scott D." last="Tanner">Scott D. Tanner</name>
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<series><title level="j">Journal of immunological methods</title>
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<front><div type="abstract" xml:lang="en"><p id="P1">We have investigated the possibility of using element-tagged antibodies for protein detection and quantification in microplate format using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), and compared the results to conventional immunoassays, such as Enzyme-Linked Immunosorbent Assay (ELISA) and Western blotting. The technique was further employed to detect low levels and measure DNA-binding activity of transcription factor p53 in leukemia cell lysates through its interaction with immobilized oligonucleotides and recognition by element-tagged antibodies. The advantages of ICP-MS detection for routine performance of immunoassays include increased sensitivity, wide dynamic range, minimal interference from complex matrices, and high throughput. Our approach advances the ICP-MS technology and demonstrates its applicability to proteomic studies through the use of antibodies directly labeled with polymer tags bearing multiple atoms of lanthanides. Development of this novel methodology will enable fast and quantitative identification of multiple analytes in a single well.</p>
</div>
</front>
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  <pmc-dir>properties manuscript</pmc-dir>
  <front><journal-meta><journal-id journal-id-type="nlm-journal-id">1305440</journal-id>
<journal-id journal-id-type="pubmed-jr-id">4818</journal-id>
<journal-id journal-id-type="nlm-ta">J Immunol Methods</journal-id>
<journal-title>Journal of immunological methods</journal-title>
<issn pub-type="ppub">0022-1759</issn>
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<article-meta><article-id pub-id-type="pmid">18456275</article-id>
<article-id pub-id-type="pmc">2583136</article-id>
<article-id pub-id-type="manuscript">NIHMS55834</article-id>
<article-id pub-id-type="doi">10.1016/j.jim.2008.03.011</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
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<title-group><article-title>Element-tagged immunoassay with ICP-MS detection: evaluation and comparison to conventional immunoassays</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Razumienko</surname>
<given-names>Eva</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
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<contrib contrib-type="author"><name><surname>Ornatsky</surname>
<given-names>Olga</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
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<contrib contrib-type="author"><name><surname>Kinach</surname>
<given-names>Robert</given-names>
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<contrib contrib-type="author"><name><surname>Milyavsky</surname>
<given-names>Michael</given-names>
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<xref ref-type="aff" rid="A2">2</xref>
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<contrib contrib-type="author"><name><surname>Lechman</surname>
<given-names>Eric</given-names>
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<xref ref-type="aff" rid="A2">2</xref>
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<contrib contrib-type="author"><name><surname>Baranov</surname>
<given-names>Vladimir</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Winnik</surname>
<given-names>Mitchell A.</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Tanner</surname>
<given-names>Scott D.</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>1</label>
Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</aff>
<aff id="A2"><label>2</label>
Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada.</aff>
<author-notes><corresp id="cor1"><label>*</label>
Corresponding author. Chemistry Department, University of Toronto, 80 St. George st. Room LM18, Toronto, ON M5S 3H6, Canada, Tel: 416 946 84 20, Fax: 416 978 8775, E-mail address: <email>olga.ornatsky@utoronto.ca</email>
</corresp>
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<pub-date pub-type="nihms-submitted"><day>29</day>
<month>7</month>
<year>2008</year>
</pub-date>
<pub-date pub-type="epub"><day>22</day>
<month>4</month>
<year>2008</year>
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<pub-date pub-type="ppub"><day>20</day>
<month>7</month>
<year>2008</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>20</day>
<month>7</month>
<year>2009</year>
</pub-date>
<volume>336</volume>
<issue>1</issue>
<fpage>56</fpage>
<lpage>63</lpage>
<abstract><p id="P1">We have investigated the possibility of using element-tagged antibodies for protein detection and quantification in microplate format using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), and compared the results to conventional immunoassays, such as Enzyme-Linked Immunosorbent Assay (ELISA) and Western blotting. The technique was further employed to detect low levels and measure DNA-binding activity of transcription factor p53 in leukemia cell lysates through its interaction with immobilized oligonucleotides and recognition by element-tagged antibodies. The advantages of ICP-MS detection for routine performance of immunoassays include increased sensitivity, wide dynamic range, minimal interference from complex matrices, and high throughput. Our approach advances the ICP-MS technology and demonstrates its applicability to proteomic studies through the use of antibodies directly labeled with polymer tags bearing multiple atoms of lanthanides. Development of this novel methodology will enable fast and quantitative identification of multiple analytes in a single well.</p>
</abstract>
<kwd-group><kwd>ELISA</kwd>
<kwd>ICP-MS</kwd>
<kwd>element-tagged antibody</kwd>
<kwd>lanthanides</kwd>
<kwd>growth factors</kwd>
<kwd>transcription factor p53</kwd>
</kwd-group>
<contract-num rid="GM1">R01 GM076127-02</contract-num>
<contract-num rid="GM1">R01 GM076127-01A1</contract-num>
<contract-sponsor id="GM1">National Institute of General Medical Sciences : NIGMS</contract-sponsor>
</article-meta>
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Element-tagged immunoassay with ICP-MS detection: evaluation and comparison to conventional immunoassays

Element-tagged immunoassay with ICP-MS detection: evaluation and comparison to conventional immunoassays

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