Spontaneously Formed Monodisperse Biomimetic Unilamellar Vesicles: The Effect of Charge, Dilution, and Time
Identifieur interne : 000368 ( Pmc/Checkpoint ); précédent : 000367; suivant : 000369Spontaneously Formed Monodisperse Biomimetic Unilamellar Vesicles: The Effect of Charge, Dilution, and Time
Auteurs : M.-P. Nieh [Canada] ; T. A. Harroun [Canada] ; V. A. Raghunathan [Inde] ; C. J. Glinka [États-Unis] ; J. Katsaras [Canada]Source :
- Biophysical Journal [ 0006-3495 ] ; 2004.
Abstract
Using small-angle neutron scattering and dynamic light scattering, we have constructed partial structural phase diagrams of lipid mixtures composed of the phosphatidylcholines dimyristoyl and dihexanoyl doped with calcium ions (Ca2+) and/or the negatively charged lipid, dimyristoyl phosphatidylglycerol (DMPG). For dilute solutions (lipid concentration ≤1 wt %), spontaneously forming unilamellar vesicles (ULVs) were found, and their polydispersity was determined to be ∼20%. The stability of the Ca2+- or DMPG-doped ULVs was monitored over a period of 4 days and their structural parameters (e.g., average outer radius, 〈
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PubMed: 15041697
PubMed Central: 1304108
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<front><div type="abstract" xml:lang="en"><p>Using small-angle neutron scattering and dynamic light scattering, we have constructed partial structural phase diagrams of lipid mixtures composed of the phosphatidylcholines dimyristoyl and dihexanoyl doped with calcium ions (Ca<sup>2+</sup>
) and/or the negatively charged lipid, dimyristoyl phosphatidylglycerol (DMPG). For dilute solutions (lipid concentration ≤1 wt %), spontaneously forming unilamellar vesicles (ULVs) were found, and their polydispersity was determined to be ∼20%. The stability of the Ca<sup>2+</sup>
- or DMPG-doped ULVs was monitored over a period of 4 days and their structural parameters (e.g., average outer radius, 〈<italic>R</italic>
<sub>o</sub>
〉) were found to be insensitive to the lipid concentration (<italic>C</italic>
<sub>lp</sub>
). However, doping the dimyristoyl/dihexanoyl system with both Ca<sup>2+</sup>
and DMPG resulted in ULVs whose 〈<italic>R</italic>
<sub>o</sub>
〉 was found to be <italic>C</italic>
<sub>lp</sub>
dependent. The 〈<italic>R</italic>
<sub>o</sub>
〉 of DMPG-doped ULVs remained unchanged over an extended period of time (at least 4 days), a good indication of their stability.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Biophys J</journal-id>
<journal-id journal-id-type="publisher-id">biophysj</journal-id>
<journal-title>Biophysical Journal</journal-title>
<issn pub-type="ppub">0006-3495</issn>
<issn pub-type="epub">1542-0086</issn>
<publisher><publisher-name>Biophysical Society</publisher-name>
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<article-id pub-id-type="publisher-id">34645</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Other</subject>
</subj-group>
</article-categories>
<title-group><article-title>Spontaneously Formed Monodisperse Biomimetic Unilamellar Vesicles: The Effect of Charge, Dilution, and Time</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Nieh</surname>
<given-names>M.-P.</given-names>
</name>
<xref ref-type="aff" rid="N0xa408218.0xa74bb88">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Harroun</surname>
<given-names>T. A.</given-names>
</name>
<xref ref-type="aff" rid="N0xa408218.0xa74bb88">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Raghunathan</surname>
<given-names>V. A.</given-names>
</name>
<xref ref-type="aff" rid="N0xa408218.0xa74bb88">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Glinka</surname>
<given-names>C. J.</given-names>
</name>
<xref ref-type="aff" rid="N0xa408218.0xa74bb88">‡</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Katsaras</surname>
<given-names>J.</given-names>
</name>
<xref ref-type="aff" rid="N0xa408218.0xa74bb88">*</xref>
</contrib>
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<aff id="N0xa408218.0xa74bb88"><label>*</label>
National Research Council Canada, Steacie Institute for Molecular Sciences, Chalk River, Ontario K0J 1J0, Canada;<label>†</label>
Raman Research Institute, Bangalore-560 080, India; and<label>‡</label>
Center for Neutron Research, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 USA</aff>
<author-notes><fn><p>Address reprint requests to John Katsaras, National Research Council Canada, Steacie Institute for Molecular Sciences, Chalk River, Ontario K0J 1J0, Canada. Tel.: 613-584-3311 ext. 3984; E-mail: <email>john.katsaras@nrc.ca</email>
.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>4</month>
<year>2004</year>
</pub-date>
<volume>86</volume>
<issue>4</issue>
<fpage>2615</fpage>
<lpage>2629</lpage>
<history><date date-type="received"><day>3</day>
<month>10</month>
<year>2003</year>
</date>
<date date-type="accepted"><day>17</day>
<month>12</month>
<year>2003</year>
</date>
</history>
<copyright-statement>Copyright © 2004, Biophysical Society</copyright-statement>
<copyright-year>2004</copyright-year>
<abstract><p>Using small-angle neutron scattering and dynamic light scattering, we have constructed partial structural phase diagrams of lipid mixtures composed of the phosphatidylcholines dimyristoyl and dihexanoyl doped with calcium ions (Ca<sup>2+</sup>
) and/or the negatively charged lipid, dimyristoyl phosphatidylglycerol (DMPG). For dilute solutions (lipid concentration ≤1 wt %), spontaneously forming unilamellar vesicles (ULVs) were found, and their polydispersity was determined to be ∼20%. The stability of the Ca<sup>2+</sup>
- or DMPG-doped ULVs was monitored over a period of 4 days and their structural parameters (e.g., average outer radius, 〈<italic>R</italic>
<sub>o</sub>
〉) were found to be insensitive to the lipid concentration (<italic>C</italic>
<sub>lp</sub>
). However, doping the dimyristoyl/dihexanoyl system with both Ca<sup>2+</sup>
and DMPG resulted in ULVs whose 〈<italic>R</italic>
<sub>o</sub>
〉 was found to be <italic>C</italic>
<sub>lp</sub>
dependent. The 〈<italic>R</italic>
<sub>o</sub>
〉 of DMPG-doped ULVs remained unchanged over an extended period of time (at least 4 days), a good indication of their stability.</p>
</abstract>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Canada</li>
<li>Inde</li>
<li>États-Unis</li>
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<region><li>Maryland</li>
</region>
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<tree><country name="Canada"><noRegion><name sortKey="Nieh, M P" sort="Nieh, M P" uniqKey="Nieh M" first="M.-P." last="Nieh">M.-P. Nieh</name>
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<name sortKey="Harroun, T A" sort="Harroun, T A" uniqKey="Harroun T" first="T. A." last="Harroun">T. A. Harroun</name>
<name sortKey="Katsaras, J" sort="Katsaras, J" uniqKey="Katsaras J" first="J." last="Katsaras">J. Katsaras</name>
</country>
<country name="Inde"><noRegion><name sortKey="Raghunathan, V A" sort="Raghunathan, V A" uniqKey="Raghunathan V" first="V. A." last="Raghunathan">V. A. Raghunathan</name>
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</country>
<country name="États-Unis"><region name="Maryland"><name sortKey="Glinka, C J" sort="Glinka, C J" uniqKey="Glinka C" first="C. J." last="Glinka">C. J. Glinka</name>
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