Spontaneously Formed Monodisperse Biomimetic Unilamellar Vesicles: The Effect of Charge, Dilution, and Time
Identifieur interne : 000134 ( Pmc/Corpus ); précédent : 000133; suivant : 000135Spontaneously Formed Monodisperse Biomimetic Unilamellar Vesicles: The Effect of Charge, Dilution, and Time
Auteurs : M.-P. Nieh ; T. A. Harroun ; V. A. Raghunathan ; C. J. Glinka ; J. KatsarasSource :
- Biophysical Journal [ 0006-3495 ] ; 2004.
Abstract
Using small-angle neutron scattering and dynamic light scattering, we have constructed partial structural phase diagrams of lipid mixtures composed of the phosphatidylcholines dimyristoyl and dihexanoyl doped with calcium ions (Ca2+) and/or the negatively charged lipid, dimyristoyl phosphatidylglycerol (DMPG). For dilute solutions (lipid concentration ≤1 wt %), spontaneously forming unilamellar vesicles (ULVs) were found, and their polydispersity was determined to be ∼20%. The stability of the Ca2+- or DMPG-doped ULVs was monitored over a period of 4 days and their structural parameters (e.g., average outer radius, 〈
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PubMed: 15041697
PubMed Central: 1304108
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Spontaneously Formed Monodisperse Biomimetic Unilamellar Vesicles: The Effect of Charge, Dilution, and Time</title><author><name sortKey="Nieh, M P" sort="Nieh, M P" uniqKey="Nieh M" first="M.-P." last="Nieh">M.-P. Nieh</name><affiliation><nlm:aff id="N0xa408218.0xa74bb88">National Research Council Canada, Steacie Institute for Molecular Sciences, Chalk River, Ontario K0J 1J0, Canada;</nlm:aff></affiliation></author><author><name sortKey="Harroun, T A" sort="Harroun, T A" uniqKey="Harroun T" first="T. A." last="Harroun">T. A. Harroun</name><affiliation><nlm:aff id="N0xa408218.0xa74bb88">National Research Council Canada, Steacie Institute for Molecular Sciences, Chalk River, Ontario K0J 1J0, Canada;</nlm:aff></affiliation></author><author><name sortKey="Raghunathan, V A" sort="Raghunathan, V A" uniqKey="Raghunathan V" first="V. A." last="Raghunathan">V. A. Raghunathan</name><affiliation><nlm:aff wicri:cut="; and" id="N0xa408218.0xa74bb88">Raman Research Institute, Bangalore-560 080, India</nlm:aff></affiliation></author><author><name sortKey="Glinka, C J" sort="Glinka, C J" uniqKey="Glinka C" first="C. J." last="Glinka">C. J. Glinka</name><affiliation><nlm:aff id="N0xa408218.0xa74bb88">Center for Neutron Research, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 USA</nlm:aff></affiliation></author><author><name sortKey="Katsaras, J" sort="Katsaras, J" uniqKey="Katsaras J" first="J." last="Katsaras">J. Katsaras</name><affiliation><nlm:aff id="N0xa408218.0xa74bb88">National Research Council Canada, Steacie Institute for Molecular Sciences, Chalk River, Ontario K0J 1J0, Canada;</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15041697</idno><idno type="pmc">1304108</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1304108</idno><idno type="RBID">PMC:1304108</idno><date when="2004">2004</date><idno type="wicri:Area/Pmc/Corpus">000134</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000134</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Spontaneously Formed Monodisperse Biomimetic Unilamellar Vesicles: The Effect of Charge, Dilution, and Time</title><author><name sortKey="Nieh, M P" sort="Nieh, M P" uniqKey="Nieh M" first="M.-P." last="Nieh">M.-P. Nieh</name><affiliation><nlm:aff id="N0xa408218.0xa74bb88">National Research Council Canada, Steacie Institute for Molecular Sciences, Chalk River, Ontario K0J 1J0, Canada;</nlm:aff></affiliation></author><author><name sortKey="Harroun, T A" sort="Harroun, T A" uniqKey="Harroun T" first="T. A." last="Harroun">T. A. Harroun</name><affiliation><nlm:aff id="N0xa408218.0xa74bb88">National Research Council Canada, Steacie Institute for Molecular Sciences, Chalk River, Ontario K0J 1J0, Canada;</nlm:aff></affiliation></author><author><name sortKey="Raghunathan, V A" sort="Raghunathan, V A" uniqKey="Raghunathan V" first="V. A." last="Raghunathan">V. A. Raghunathan</name><affiliation><nlm:aff wicri:cut="; and" id="N0xa408218.0xa74bb88">Raman Research Institute, Bangalore-560 080, India</nlm:aff></affiliation></author><author><name sortKey="Glinka, C J" sort="Glinka, C J" uniqKey="Glinka C" first="C. J." last="Glinka">C. J. Glinka</name><affiliation><nlm:aff id="N0xa408218.0xa74bb88">Center for Neutron Research, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 USA</nlm:aff></affiliation></author><author><name sortKey="Katsaras, J" sort="Katsaras, J" uniqKey="Katsaras J" first="J." last="Katsaras">J. Katsaras</name><affiliation><nlm:aff id="N0xa408218.0xa74bb88">National Research Council Canada, Steacie Institute for Molecular Sciences, Chalk River, Ontario K0J 1J0, Canada;</nlm:aff></affiliation></author></analytic><series><title level="j">Biophysical Journal</title><idno type="ISSN">0006-3495</idno><idno type="eISSN">1542-0086</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Using small-angle neutron scattering and dynamic light scattering, we have constructed partial structural phase diagrams of lipid mixtures composed of the phosphatidylcholines dimyristoyl and dihexanoyl doped with calcium ions (Ca<sup>2+</sup>) and/or the negatively charged lipid, dimyristoyl phosphatidylglycerol (DMPG). For dilute solutions (lipid concentration ≤1 wt %), spontaneously forming unilamellar vesicles (ULVs) were found, and their polydispersity was determined to be ∼20%. The stability of the Ca<sup>2+</sup>- or DMPG-doped ULVs was monitored over a period of 4 days and their structural parameters (e.g., average outer radius, 〈<italic>R</italic><sub>o</sub>〉) were found to be insensitive to the lipid concentration (<italic>C</italic><sub>lp</sub>). However, doping the dimyristoyl/dihexanoyl system with both Ca<sup>2+</sup> and DMPG resulted in ULVs whose 〈<italic>R</italic><sub>o</sub>〉 was found to be <italic>C</italic><sub>lp</sub> dependent. The 〈<italic>R</italic><sub>o</sub>〉 of DMPG-doped ULVs remained unchanged over an extended period of time (at least 4 days), a good indication of their stability.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Biophys J</journal-id><journal-id journal-id-type="publisher-id">biophysj</journal-id><journal-title>Biophysical Journal</journal-title><issn pub-type="ppub">0006-3495</issn><issn pub-type="epub">1542-0086</issn><publisher><publisher-name>Biophysical Society</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15041697</article-id><article-id pub-id-type="pmc">1304108</article-id><article-id pub-id-type="publisher-id">34645</article-id><article-categories><subj-group subj-group-type="heading"><subject>Other</subject></subj-group></article-categories><title-group><article-title>Spontaneously Formed Monodisperse Biomimetic Unilamellar Vesicles: The Effect of Charge, Dilution, and Time</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Nieh</surname><given-names>M.-P.</given-names></name><xref ref-type="aff" rid="N0xa408218.0xa74bb88">*</xref></contrib><contrib contrib-type="author"><name><surname>Harroun</surname><given-names>T. A.</given-names></name><xref ref-type="aff" rid="N0xa408218.0xa74bb88">*</xref></contrib><contrib contrib-type="author"><name><surname>Raghunathan</surname><given-names>V. A.</given-names></name><xref ref-type="aff" rid="N0xa408218.0xa74bb88">†</xref></contrib><contrib contrib-type="author"><name><surname>Glinka</surname><given-names>C. J.</given-names></name><xref ref-type="aff" rid="N0xa408218.0xa74bb88">‡</xref></contrib><contrib contrib-type="author"><name><surname>Katsaras</surname><given-names>J.</given-names></name><xref ref-type="aff" rid="N0xa408218.0xa74bb88">*</xref></contrib></contrib-group><aff id="N0xa408218.0xa74bb88"><label>*</label>National Research Council Canada, Steacie Institute for Molecular Sciences, Chalk River, Ontario K0J 1J0, Canada;<label>†</label>Raman Research Institute, Bangalore-560 080, India; and<label>‡</label>Center for Neutron Research, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 USA</aff><author-notes><fn><p>Address reprint requests to John Katsaras, National Research Council Canada, Steacie Institute for Molecular Sciences, Chalk River, Ontario K0J 1J0, Canada. Tel.: 613-584-3311 ext. 3984; E-mail: <email>john.katsaras@nrc.ca</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>4</month><year>2004</year></pub-date><volume>86</volume><issue>4</issue><fpage>2615</fpage><lpage>2629</lpage><history><date date-type="received"><day>3</day><month>10</month><year>2003</year></date><date date-type="accepted"><day>17</day><month>12</month><year>2003</year></date></history><copyright-statement>Copyright © 2004, Biophysical Society</copyright-statement><copyright-year>2004</copyright-year><abstract><p>Using small-angle neutron scattering and dynamic light scattering, we have constructed partial structural phase diagrams of lipid mixtures composed of the phosphatidylcholines dimyristoyl and dihexanoyl doped with calcium ions (Ca<sup>2+</sup>) and/or the negatively charged lipid, dimyristoyl phosphatidylglycerol (DMPG). For dilute solutions (lipid concentration ≤1 wt %), spontaneously forming unilamellar vesicles (ULVs) were found, and their polydispersity was determined to be ∼20%. The stability of the Ca<sup>2+</sup>- or DMPG-doped ULVs was monitored over a period of 4 days and their structural parameters (e.g., average outer radius, 〈<italic>R</italic><sub>o</sub>〉) were found to be insensitive to the lipid concentration (<italic>C</italic><sub>lp</sub>). However, doping the dimyristoyl/dihexanoyl system with both Ca<sup>2+</sup> and DMPG resulted in ULVs whose 〈<italic>R</italic><sub>o</sub>〉 was found to be <italic>C</italic><sub>lp</sub> dependent. The 〈<italic>R</italic><sub>o</sub>〉 of DMPG-doped ULVs remained unchanged over an extended period of time (at least 4 days), a good indication of their stability.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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