Stoichiometric Relationship between Na+ Ions Transported and Glucose Consumed in Human Erythrocytes: Bayesian Analysis of 23Na and 13C NMR Time Course Data
Identifieur interne : 000671 ( Ncbi/Checkpoint ); précédent : 000670; suivant : 000672Stoichiometric Relationship between Na+ Ions Transported and Glucose Consumed in Human Erythrocytes: Bayesian Analysis of 23Na and 13C NMR Time Course Data
Auteurs : Max Puckeridge [Australie] ; Bogdan E. Chapman [Australie] ; Arthur D. Conigrave [Australie] ; Stuart M. Grieve [Australie] ; Gemma A. Figtree [Australie] ; Philip W. Kuchel [Australie]Source :
- Biophysical Journal [ 0006-3495 ] ; 2013.
Abstract
We examined the response of Na+,K+-ATPase (NKA) to monensin, a Na+ ionophore, with and without ouabain, an NKA inhibitor, in suspensions of human erythrocytes (red blood cells). A combination of 13C and 23Na NMR methods allowed the recording of intra- and extracellular Na+, and 13C-labeled glucose time courses. The net influx of Na+ and the consumption of glucose were measured with and without NKA inhibited by ouabain. A Bayesian analysis was used to determine probability distributions of the parameter values of a minimalist mathematical model of the kinetics involved, and then used to infer the rates of Na+ transported and glucose consumed. It was estimated that the numerical relationship between the number of Na+ ions transported by NKA per molecule of glucose consumed by a red blood cell was close to the ratio 6.0:1.0, agreeing with theoretical prediction.
Url:
DOI: 10.1016/j.bpj.2013.03.019
PubMed: 23601315
PubMed Central: 3628572
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000264
- to stream Pmc, to step Curation: 000264
- to stream Pmc, to step Checkpoint: 000171
- to stream Ncbi, to step Merge: 000671
- to stream Ncbi, to step Curation: 000671
Links to Exploration step
PMC:3628572Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Stoichiometric Relationship between Na<sup>+</sup> Ions Transported and Glucose Consumed in Human Erythrocytes: Bayesian Analysis of <sup>23</sup>Na and <sup>13</sup>C NMR Time Course Data</title><author><name sortKey="Puckeridge, Max" sort="Puckeridge, Max" uniqKey="Puckeridge M" first="Max" last="Puckeridge">Max Puckeridge</name><affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Chapman, Bogdan E" sort="Chapman, Bogdan E" uniqKey="Chapman B" first="Bogdan E." last="Chapman">Bogdan E. Chapman</name><affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Conigrave, Arthur D" sort="Conigrave, Arthur D" uniqKey="Conigrave A" first="Arthur D." last="Conigrave">Arthur D. Conigrave</name><affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Grieve, Stuart M" sort="Grieve, Stuart M" uniqKey="Grieve S" first="Stuart M." last="Grieve">Stuart M. Grieve</name><affiliation wicri:level="3"><nlm:aff id="aff2">North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff3">Department of Radiology, Royal Prince Alfred Hospital, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Radiology, Royal Prince Alfred Hospital, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Figtree, Gemma A" sort="Figtree, Gemma A" uniqKey="Figtree G" first="Gemma A." last="Figtree">Gemma A. Figtree</name><affiliation wicri:level="3"><nlm:aff id="aff2">North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff4">Department of Cardiology, Royal North Shore Hospital, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Cardiology, Royal North Shore Hospital, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Kuchel, Philip W" sort="Kuchel, Philip W" uniqKey="Kuchel P" first="Philip W." last="Kuchel">Philip W. Kuchel</name><affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23601315</idno><idno type="pmc">3628572</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3628572</idno><idno type="RBID">PMC:3628572</idno><idno type="doi">10.1016/j.bpj.2013.03.019</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000264</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000264</idno><idno type="wicri:Area/Pmc/Curation">000264</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000264</idno><idno type="wicri:Area/Pmc/Checkpoint">000171</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000171</idno><idno type="wicri:Area/Ncbi/Merge">000671</idno><idno type="wicri:Area/Ncbi/Curation">000671</idno><idno type="wicri:Area/Ncbi/Checkpoint">000671</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Stoichiometric Relationship between Na<sup>+</sup> Ions Transported and Glucose Consumed in Human Erythrocytes: Bayesian Analysis of <sup>23</sup>Na and <sup>13</sup>C NMR Time Course Data</title><author><name sortKey="Puckeridge, Max" sort="Puckeridge, Max" uniqKey="Puckeridge M" first="Max" last="Puckeridge">Max Puckeridge</name><affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Chapman, Bogdan E" sort="Chapman, Bogdan E" uniqKey="Chapman B" first="Bogdan E." last="Chapman">Bogdan E. Chapman</name><affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Conigrave, Arthur D" sort="Conigrave, Arthur D" uniqKey="Conigrave A" first="Arthur D." last="Conigrave">Arthur D. Conigrave</name><affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Grieve, Stuart M" sort="Grieve, Stuart M" uniqKey="Grieve S" first="Stuart M." last="Grieve">Stuart M. Grieve</name><affiliation wicri:level="3"><nlm:aff id="aff2">North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff3">Department of Radiology, Royal Prince Alfred Hospital, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Radiology, Royal Prince Alfred Hospital, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Figtree, Gemma A" sort="Figtree, Gemma A" uniqKey="Figtree G" first="Gemma A." last="Figtree">Gemma A. Figtree</name><affiliation wicri:level="3"><nlm:aff id="aff2">North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff4">Department of Cardiology, Royal North Shore Hospital, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Cardiology, Royal North Shore Hospital, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Kuchel, Philip W" sort="Kuchel, Philip W" uniqKey="Kuchel P" first="Philip W." last="Kuchel">Philip W. Kuchel</name><affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author></analytic><series><title level="j">Biophysical Journal</title><idno type="ISSN">0006-3495</idno><idno type="eISSN">1542-0086</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We examined the response of Na<sup>+</sup>,K<sup>+</sup>-ATPase (NKA) to monensin, a Na<sup>+</sup> ionophore, with and without ouabain, an NKA inhibitor, in suspensions of human erythrocytes (red blood cells). A combination of <sup>13</sup>C and <sup>23</sup>Na NMR methods allowed the recording of intra- and extracellular Na<sup>+</sup>, and <sup>13</sup>C-labeled glucose time courses. The net influx of Na<sup>+</sup> and the consumption of glucose were measured with and without NKA inhibited by ouabain. A Bayesian analysis was used to determine probability distributions of the parameter values of a minimalist mathematical model of the kinetics involved, and then used to infer the rates of Na<sup>+</sup> transported and glucose consumed. It was estimated that the numerical relationship between the number of Na<sup>+</sup> ions transported by NKA per molecule of glucose consumed by a red blood cell was close to the ratio 6.0:1.0, agreeing with theoretical prediction.</p></div></front></TEI><affiliations><list><country><li>Australie</li></country><region><li>Nouvelle-Galles du Sud</li></region><settlement><li>Sydney</li></settlement></list><tree><country name="Australie"><region name="Nouvelle-Galles du Sud"><name sortKey="Puckeridge, Max" sort="Puckeridge, Max" uniqKey="Puckeridge M" first="Max" last="Puckeridge">Max Puckeridge</name></region><name sortKey="Chapman, Bogdan E" sort="Chapman, Bogdan E" uniqKey="Chapman B" first="Bogdan E." last="Chapman">Bogdan E. Chapman</name><name sortKey="Conigrave, Arthur D" sort="Conigrave, Arthur D" uniqKey="Conigrave A" first="Arthur D." last="Conigrave">Arthur D. Conigrave</name><name sortKey="Figtree, Gemma A" sort="Figtree, Gemma A" uniqKey="Figtree G" first="Gemma A." last="Figtree">Gemma A. Figtree</name><name sortKey="Figtree, Gemma A" sort="Figtree, Gemma A" uniqKey="Figtree G" first="Gemma A." last="Figtree">Gemma A. Figtree</name><name sortKey="Grieve, Stuart M" sort="Grieve, Stuart M" uniqKey="Grieve S" first="Stuart M." last="Grieve">Stuart M. Grieve</name><name sortKey="Grieve, Stuart M" sort="Grieve, Stuart M" uniqKey="Grieve S" first="Stuart M." last="Grieve">Stuart M. Grieve</name><name sortKey="Kuchel, Philip W" sort="Kuchel, Philip W" uniqKey="Kuchel P" first="Philip W." last="Kuchel">Philip W. Kuchel</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/ThuliumV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000671 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000671 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Terre
|area= ThuliumV1
|flux= Ncbi
|étape= Checkpoint
|type= RBID
|clé= PMC:3628572
|texte= Stoichiometric Relationship between Na+ Ions Transported and Glucose Consumed in Human Erythrocytes: Bayesian Analysis of 23Na and 13C NMR Time Course Data
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:23601315" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a ThuliumV1
| This area was generated with Dilib version V0.6.21. |  |