Stoichiometric Relationship between Na+ Ions Transported and Glucose Consumed in Human Erythrocytes: Bayesian Analysis of 23Na and 13C NMR Time Course Data
Identifieur interne : 000671 ( Ncbi/Checkpoint ); précédent : 000670; suivant : 000672Stoichiometric Relationship between Na+ Ions Transported and Glucose Consumed in Human Erythrocytes: Bayesian Analysis of 23Na and 13C NMR Time Course Data
Auteurs : Max Puckeridge [Australie] ; Bogdan E. Chapman [Australie] ; Arthur D. Conigrave [Australie] ; Stuart M. Grieve [Australie] ; Gemma A. Figtree [Australie] ; Philip W. Kuchel [Australie]Source :
- Biophysical Journal [ 0006-3495 ] ; 2013.
Abstract
We examined the response of Na+,K+-ATPase (NKA) to monensin, a Na+ ionophore, with and without ouabain, an NKA inhibitor, in suspensions of human erythrocytes (red blood cells). A combination of 13C and 23Na NMR methods allowed the recording of intra- and extracellular Na+, and 13C-labeled glucose time courses. The net influx of Na+ and the consumption of glucose were measured with and without NKA inhibited by ouabain. A Bayesian analysis was used to determine probability distributions of the parameter values of a minimalist mathematical model of the kinetics involved, and then used to infer the rates of Na+ transported and glucose consumed. It was estimated that the numerical relationship between the number of Na+ ions transported by NKA per molecule of glucose consumed by a red blood cell was close to the ratio 6.0:1.0, agreeing with theoretical prediction.
Url:
DOI: 10.1016/j.bpj.2013.03.019
PubMed: 23601315
PubMed Central: 3628572
Affiliations:
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C NMR Time Course Data</title>
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Na and <sup>13</sup>
C NMR Time Course Data</title>
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<front><div type="abstract" xml:lang="en"><p>We examined the response of Na<sup>+</sup>
,K<sup>+</sup>
-ATPase (NKA) to monensin, a Na<sup>+</sup>
ionophore, with and without ouabain, an NKA inhibitor, in suspensions of human erythrocytes (red blood cells). A combination of <sup>13</sup>
C and <sup>23</sup>
Na NMR methods allowed the recording of intra- and extracellular Na<sup>+</sup>
, and <sup>13</sup>
C-labeled glucose time courses. The net influx of Na<sup>+</sup>
and the consumption of glucose were measured with and without NKA inhibited by ouabain. A Bayesian analysis was used to determine probability distributions of the parameter values of a minimalist mathematical model of the kinetics involved, and then used to infer the rates of Na<sup>+</sup>
transported and glucose consumed. It was estimated that the numerical relationship between the number of Na<sup>+</sup>
ions transported by NKA per molecule of glucose consumed by a red blood cell was close to the ratio 6.0:1.0, agreeing with theoretical prediction.</p>
</div>
</front>
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<name sortKey="Chapman, Bogdan E" sort="Chapman, Bogdan E" uniqKey="Chapman B" first="Bogdan E." last="Chapman">Bogdan E. Chapman</name>
<name sortKey="Conigrave, Arthur D" sort="Conigrave, Arthur D" uniqKey="Conigrave A" first="Arthur D." last="Conigrave">Arthur D. Conigrave</name>
<name sortKey="Figtree, Gemma A" sort="Figtree, Gemma A" uniqKey="Figtree G" first="Gemma A." last="Figtree">Gemma A. Figtree</name>
<name sortKey="Figtree, Gemma A" sort="Figtree, Gemma A" uniqKey="Figtree G" first="Gemma A." last="Figtree">Gemma A. Figtree</name>
<name sortKey="Grieve, Stuart M" sort="Grieve, Stuart M" uniqKey="Grieve S" first="Stuart M." last="Grieve">Stuart M. Grieve</name>
<name sortKey="Grieve, Stuart M" sort="Grieve, Stuart M" uniqKey="Grieve S" first="Stuart M." last="Grieve">Stuart M. Grieve</name>
<name sortKey="Kuchel, Philip W" sort="Kuchel, Philip W" uniqKey="Kuchel P" first="Philip W." last="Kuchel">Philip W. Kuchel</name>
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