Stoichiometric Relationship between Na+ Ions Transported and Glucose Consumed in Human Erythrocytes: Bayesian Analysis of 23Na and 13C NMR Time Course Data
Identifieur interne : 000671 ( Ncbi/Curation ); précédent : 000670; suivant : 000672Stoichiometric Relationship between Na+ Ions Transported and Glucose Consumed in Human Erythrocytes: Bayesian Analysis of 23Na and 13C NMR Time Course Data
Auteurs : Max Puckeridge [Australie] ; Bogdan E. Chapman [Australie] ; Arthur D. Conigrave [Australie] ; Stuart M. Grieve [Australie] ; Gemma A. Figtree [Australie] ; Philip W. Kuchel [Australie]Source :
- Biophysical Journal [ 0006-3495 ] ; 2013.
Abstract
We examined the response of Na+,K+-ATPase (NKA) to monensin, a Na+ ionophore, with and without ouabain, an NKA inhibitor, in suspensions of human erythrocytes (red blood cells). A combination of 13C and 23Na NMR methods allowed the recording of intra- and extracellular Na+, and 13C-labeled glucose time courses. The net influx of Na+ and the consumption of glucose were measured with and without NKA inhibited by ouabain. A Bayesian analysis was used to determine probability distributions of the parameter values of a minimalist mathematical model of the kinetics involved, and then used to infer the rates of Na+ transported and glucose consumed. It was estimated that the numerical relationship between the number of Na+ ions transported by NKA per molecule of glucose consumed by a red blood cell was close to the ratio 6.0:1.0, agreeing with theoretical prediction.
Url:
DOI: 10.1016/j.bpj.2013.03.019
PubMed: 23601315
PubMed Central: 3628572
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000264
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :000264
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000171
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000671
Links to Exploration step
PMC:3628572Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Stoichiometric Relationship between Na<sup>+</sup>
Ions Transported and Glucose Consumed in Human Erythrocytes: Bayesian Analysis of <sup>23</sup>
Na and <sup>13</sup>
C NMR Time Course Data</title>
<author><name sortKey="Puckeridge, Max" sort="Puckeridge, Max" uniqKey="Puckeridge M" first="Max" last="Puckeridge">Max Puckeridge</name>
<affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chapman, Bogdan E" sort="Chapman, Bogdan E" uniqKey="Chapman B" first="Bogdan E." last="Chapman">Bogdan E. Chapman</name>
<affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Conigrave, Arthur D" sort="Conigrave, Arthur D" uniqKey="Conigrave A" first="Arthur D." last="Conigrave">Arthur D. Conigrave</name>
<affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Grieve, Stuart M" sort="Grieve, Stuart M" uniqKey="Grieve S" first="Stuart M." last="Grieve">Stuart M. Grieve</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Radiology, Royal Prince Alfred Hospital, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Radiology, Royal Prince Alfred Hospital, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Figtree, Gemma A" sort="Figtree, Gemma A" uniqKey="Figtree G" first="Gemma A." last="Figtree">Gemma A. Figtree</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff4">Department of Cardiology, Royal North Shore Hospital, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Cardiology, Royal North Shore Hospital, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kuchel, Philip W" sort="Kuchel, Philip W" uniqKey="Kuchel P" first="Philip W." last="Kuchel">Philip W. Kuchel</name>
<affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">23601315</idno>
<idno type="pmc">3628572</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3628572</idno>
<idno type="RBID">PMC:3628572</idno>
<idno type="doi">10.1016/j.bpj.2013.03.019</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">000264</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000264</idno>
<idno type="wicri:Area/Pmc/Curation">000264</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000264</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000171</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000171</idno>
<idno type="wicri:Area/Ncbi/Merge">000671</idno>
<idno type="wicri:Area/Ncbi/Curation">000671</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Stoichiometric Relationship between Na<sup>+</sup>
Ions Transported and Glucose Consumed in Human Erythrocytes: Bayesian Analysis of <sup>23</sup>
Na and <sup>13</sup>
C NMR Time Course Data</title>
<author><name sortKey="Puckeridge, Max" sort="Puckeridge, Max" uniqKey="Puckeridge M" first="Max" last="Puckeridge">Max Puckeridge</name>
<affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chapman, Bogdan E" sort="Chapman, Bogdan E" uniqKey="Chapman B" first="Bogdan E." last="Chapman">Bogdan E. Chapman</name>
<affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Conigrave, Arthur D" sort="Conigrave, Arthur D" uniqKey="Conigrave A" first="Arthur D." last="Conigrave">Arthur D. Conigrave</name>
<affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Grieve, Stuart M" sort="Grieve, Stuart M" uniqKey="Grieve S" first="Stuart M." last="Grieve">Stuart M. Grieve</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff3">Department of Radiology, Royal Prince Alfred Hospital, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Radiology, Royal Prince Alfred Hospital, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Figtree, Gemma A" sort="Figtree, Gemma A" uniqKey="Figtree G" first="Gemma A." last="Figtree">Gemma A. Figtree</name>
<affiliation wicri:level="3"><nlm:aff id="aff2">North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>North Shore Heart Research Group, Kolling Institute, University of Sydney, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:aff id="aff4">Department of Cardiology, Royal North Shore Hospital, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Cardiology, Royal North Shore Hospital, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kuchel, Philip W" sort="Kuchel, Philip W" uniqKey="Kuchel P" first="Philip W." last="Kuchel">Philip W. Kuchel</name>
<affiliation wicri:level="3"><nlm:aff id="aff1">School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney, Australia</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Molecular Bioscience, Kolling Institute, University of Sydney, Sydney</wicri:regionArea>
<placeName><settlement type="city">Sydney</settlement>
<region type="état">Nouvelle-Galles du Sud</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Biophysical Journal</title>
<idno type="ISSN">0006-3495</idno>
<idno type="eISSN">1542-0086</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>We examined the response of Na<sup>+</sup>
,K<sup>+</sup>
-ATPase (NKA) to monensin, a Na<sup>+</sup>
ionophore, with and without ouabain, an NKA inhibitor, in suspensions of human erythrocytes (red blood cells). A combination of <sup>13</sup>
C and <sup>23</sup>
Na NMR methods allowed the recording of intra- and extracellular Na<sup>+</sup>
, and <sup>13</sup>
C-labeled glucose time courses. The net influx of Na<sup>+</sup>
and the consumption of glucose were measured with and without NKA inhibited by ouabain. A Bayesian analysis was used to determine probability distributions of the parameter values of a minimalist mathematical model of the kinetics involved, and then used to infer the rates of Na<sup>+</sup>
transported and glucose consumed. It was estimated that the numerical relationship between the number of Na<sup>+</sup>
ions transported by NKA per molecule of glucose consumed by a red blood cell was close to the ratio 6.0:1.0, agreeing with theoretical prediction.</p>
</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/ThuliumV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000671 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 000671 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Terre |area= ThuliumV1 |flux= Ncbi |étape= Curation |type= RBID |clé= PMC:3628572 |texte= Stoichiometric Relationship between Na+ Ions Transported and Glucose Consumed in Human Erythrocytes: Bayesian Analysis of 23Na and 13C NMR Time Course Data }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:23601315" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a ThuliumV1
This area was generated with Dilib version V0.6.21. |