Discrimination of Intra- and Extracellular 23Na+ Signals in Yeast Cell Suspensions Using Longitudinal Magnetic Resonance Relaxography
Identifieur interne : 000379 ( Ncbi/Checkpoint ); précédent : 000378; suivant : 000380Discrimination of Intra- and Extracellular 23Na+ Signals in Yeast Cell Suspensions Using Longitudinal Magnetic Resonance Relaxography
Auteurs : Yajie Zhang [États-Unis] ; Marie Poirer-Quinot [États-Unis] ; Charles S. Springer [États-Unis] ; James A. Balschi [États-Unis]Source :
- Journal of magnetic resonance (San Diego, Calif. : 1997) [ 1090-7807 ] ; 2010.
Abstract
This study tested the ability of MR Relaxography (MRR) to discriminate intra- (Nai+) and extracellular (Nae+) 23Na+ signals using their longitudinal relaxation time constant (T1) values. Na+-loaded yeast cell (
Url:
DOI: 10.1016/j.jmr.2010.03.018
PubMed: 20430659
PubMed Central: 2885488
Affiliations:
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Na<sup>+</sup>
Signals in Yeast Cell Suspensions Using Longitudinal Magnetic Resonance Relaxography</title>
<author><name sortKey="Zhang, Yajie" sort="Zhang, Yajie" uniqKey="Zhang Y" first="Yajie" last="Zhang">Yajie Zhang</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Discrimination of Intra- and Extracellular <sup>23</sup>
Na<sup>+</sup>
Signals in Yeast Cell Suspensions Using Longitudinal Magnetic Resonance Relaxography</title>
<author><name sortKey="Zhang, Yajie" sort="Zhang, Yajie" uniqKey="Zhang Y" first="Yajie" last="Zhang">Yajie Zhang</name>
<affiliation wicri:level="2"><nlm:aff id="A1"> Physiological NMR Core Laboratory, Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea> Physiological NMR Core Laboratory, Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Poirer Quinot, Marie" sort="Poirer Quinot, Marie" uniqKey="Poirer Quinot M" first="Marie" last="Poirer-Quinot">Marie Poirer-Quinot</name>
<affiliation wicri:level="2"><nlm:aff id="A1"> Physiological NMR Core Laboratory, Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea> Physiological NMR Core Laboratory, Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston</wicri:cityArea>
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</author>
<author><name sortKey="Springer, Charles S" sort="Springer, Charles S" uniqKey="Springer C" first="Charles S." last="Springer">Charles S. Springer</name>
<affiliation wicri:level="2"><nlm:aff id="A2"> Advanced Imaging Research Center, Oregon Health Science University, 3181 SW Sam Jackson Park Road, Mailcode L452, Portland, OR 97239</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Oregon</region>
</placeName>
<wicri:cityArea> Advanced Imaging Research Center, Oregon Health Science University, 3181 SW Sam Jackson Park Road, Mailcode L452, Portland</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Balschi, James A" sort="Balschi, James A" uniqKey="Balschi J" first="James A" last="Balschi">James A. Balschi</name>
<affiliation wicri:level="2"><nlm:aff id="A1"> Physiological NMR Core Laboratory, Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea> Physiological NMR Core Laboratory, Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston</wicri:cityArea>
</affiliation>
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<series><title level="j">Journal of magnetic resonance (San Diego, Calif. : 1997)</title>
<idno type="ISSN">1090-7807</idno>
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<front><div type="abstract" xml:lang="en"><p id="P2">This study tested the ability of MR Relaxography (MRR) to discriminate intra- (Na<sub>i</sub>
<sup>+</sup>
) and extracellular (Na<sub>e</sub>
<sup>+</sup>
) <sup>23</sup>
Na<sup>+</sup>
signals using their longitudinal relaxation time constant (T<sub>1</sub>
) values. Na<sup>+</sup>
-loaded yeast cell (<italic>Saccharomyces cerevisiae</italic>
) suspensions were investigated. Two types of compartmental <sup>23</sup>
Na<sup>+</sup>
T<sub>1</sub>
differences were examined: a selective Na<sub>e</sub>
<sup>+</sup>
T<sub>1</sub>
decrease induced by an extracellular relaxation reagent (RR<sub>e</sub>
), GdDOTP<sup>5−</sup>
; and, an intrinsic T<sub>1</sub>
difference. Parallel studies using the established method of <sup>23</sup>
Na MRS with an extracellular shift reagent (SR<sub>e</sub>
), TmDOTP<sup>5−</sup>
, were used to validate the MRR measurements. With 12.8 mM RR<sub>e</sub>
, the <sup>23</sup>
Na<sub>e</sub>
<sup>+</sup>
T<sub>1</sub>
was 2.4 ms and the <sup>23</sup>
Na<sub>i</sub>
<sup>+</sup>
T<sub>1</sub>
was 9.5 ms (9.4T, 24°C). The Na<sup>+</sup>
amounts and spontaneous efflux rate constants were found to be identical within experimental error whether measured by MRR/RR<sub>e</sub>
or by MRS/SR<sub>e</sub>
. Without RR<sub>e</sub>
, the Na<sup>+</sup>
-loaded yeast cell suspension <sup>23</sup>
Na MR signal exhibited two T<sub>1</sub>
values, 9.1 (± 0.3) ms and 32.7 (± 2.3) ms, assigned to <sup>23</sup>
Na<sub>i</sub>
<sup>+</sup>
and <sup>23</sup>
Na<sub>e</sub>
<sup>+</sup>
, respectively. The Na<sub>i</sub>
<sup>+</sup>
content measured was lower, 0.88 (± 0.06); while Na<sub>e</sub>
<sup>+</sup>
was higher, 1.43 (± 0.12) compared with MRS/SR<sub>e</sub>
measures on the same samples. However, the measured efflux rate constant was identical. T<sub>1</sub>
MRR potentially may be used for Na<sub>i</sub>
<sup>+</sup>
determination <italic>in vivo</italic>
and Na<sup>+</sup>
flux measurements; with RR<sub>e</sub>
for animal studies and without RR<sub>e</sub>
for humans.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Massachusetts</li>
<li>Oregon</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Massachusetts"><name sortKey="Zhang, Yajie" sort="Zhang, Yajie" uniqKey="Zhang Y" first="Yajie" last="Zhang">Yajie Zhang</name>
</region>
<name sortKey="Balschi, James A" sort="Balschi, James A" uniqKey="Balschi J" first="James A" last="Balschi">James A. Balschi</name>
<name sortKey="Poirer Quinot, Marie" sort="Poirer Quinot, Marie" uniqKey="Poirer Quinot M" first="Marie" last="Poirer-Quinot">Marie Poirer-Quinot</name>
<name sortKey="Springer, Charles S" sort="Springer, Charles S" uniqKey="Springer C" first="Charles S." last="Springer">Charles S. Springer</name>
</country>
</tree>
</affiliations>
</record>
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