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Discrimination of Intra- and Extracellular 23Na+ Signals in Yeast Cell Suspensions Using Longitudinal Magnetic Resonance Relaxography

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Discrimination of Intra- and Extracellular 23Na+ Signals in Yeast Cell Suspensions Using Longitudinal Magnetic Resonance Relaxography

Auteurs : Yajie Zhang [États-Unis] ; Marie Poirer-Quinot [États-Unis] ; Charles S. Springer [États-Unis] ; James A. Balschi [États-Unis]

Source :

Journal of magnetic resonance (San Diego, Calif. : 1997) [ 1090-7807 ] ; 2010.

RBID : PMC:2885488

Abstract

This study tested the ability of MR Relaxography (MRR) to discriminate intra- (Na_i⁺) and extracellular (Na_e⁺) ²³Na⁺ signals using their longitudinal relaxation time constant (T₁) values. Na⁺-loaded yeast cell (Saccharomyces cerevisiae) suspensions were investigated. Two types of compartmental ²³Na⁺ T₁ differences were examined: a selective Na_e⁺ T₁ decrease induced by an extracellular relaxation reagent (RR_e), GdDOTP⁵⁻; and, an intrinsic T₁ difference. Parallel studies using the established method of ²³Na MRS with an extracellular shift reagent (SR_e), TmDOTP⁵⁻, were used to validate the MRR measurements. With 12.8 mM RR_e, the ²³Na_e⁺ T₁ was 2.4 ms and the ²³Na_i⁺ T₁ was 9.5 ms (9.4T, 24°C). The Na⁺ amounts and spontaneous efflux rate constants were found to be identical within experimental error whether measured by MRR/RR_e or by MRS/SR_e. Without RR_e, the Na⁺-loaded yeast cell suspension ²³Na MR signal exhibited two T₁ values, 9.1 (± 0.3) ms and 32.7 (± 2.3) ms, assigned to ²³Na_i⁺ and ²³Na_e⁺, respectively. The Na_i⁺ content measured was lower, 0.88 (± 0.06); while Na_e⁺ was higher, 1.43 (± 0.12) compared with MRS/SR_e measures on the same samples. However, the measured efflux rate constant was identical. T₁ MRR potentially may be used for Na_i⁺ determination in vivo and Na⁺ flux measurements; with RR_e for animal studies and without RR_e for humans.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885488

DOI: 10.1016/j.jmr.2010.03.018
PubMed: 20430659
PubMed Central: 2885488

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Na<sup>+</sup>
 Signals in Yeast Cell Suspensions Using Longitudinal Magnetic Resonance Relaxography</title>
<author><name sortKey="Zhang, Yajie" sort="Zhang, Yajie" uniqKey="Zhang Y" first="Yajie" last="Zhang">Yajie Zhang</name>
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 Signals in Yeast Cell Suspensions Using Longitudinal Magnetic Resonance Relaxography</title>
<author><name sortKey="Zhang, Yajie" sort="Zhang, Yajie" uniqKey="Zhang Y" first="Yajie" last="Zhang">Yajie Zhang</name>
<affiliation wicri:level="2"><nlm:aff id="A1"> Physiological NMR Core Laboratory, Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115</nlm:aff>
<country xml:lang="fr">États-Unis</country>
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<author><name sortKey="Poirer Quinot, Marie" sort="Poirer Quinot, Marie" uniqKey="Poirer Quinot M" first="Marie" last="Poirer-Quinot">Marie Poirer-Quinot</name>
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<placeName><region type="state">Massachusetts</region>
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<author><name sortKey="Balschi, James A" sort="Balschi, James A" uniqKey="Balschi J" first="James A" last="Balschi">James A. Balschi</name>
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<placeName><region type="state">Massachusetts</region>
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<idno type="ISSN">1090-7807</idno>
<idno type="eISSN">1096-0856</idno>
<imprint><date when="2010">2010</date>
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<front><div type="abstract" xml:lang="en"><p id="P2">This study tested the ability of MR Relaxography (MRR) to discriminate intra- (Na<sub>i</sub>
<sup>+</sup>
) and extracellular (Na<sub>e</sub>
<sup>+</sup>
) <sup>23</sup>
Na<sup>+</sup>
 signals using their longitudinal relaxation time constant (T<sub>1</sub>
) values. Na<sup>+</sup>
-loaded yeast cell (<italic>Saccharomyces cerevisiae</italic>
) suspensions were investigated. Two types of compartmental <sup>23</sup>
Na<sup>+</sup>
 T<sub>1</sub>
 differences were examined: a selective Na<sub>e</sub>
<sup>+</sup>
 T<sub>1</sub>
 decrease induced by an extracellular relaxation reagent (RR<sub>e</sub>
), GdDOTP<sup>5−</sup>
; and, an intrinsic T<sub>1</sub>
 difference. Parallel studies using the established method of <sup>23</sup>
Na MRS with an extracellular shift reagent (SR<sub>e</sub>
), TmDOTP<sup>5−</sup>
, were used to validate the MRR measurements. With 12.8 mM RR<sub>e</sub>
, the <sup>23</sup>
Na<sub>e</sub>
<sup>+</sup>
 T<sub>1</sub>
 was 2.4 ms and the <sup>23</sup>
Na<sub>i</sub>
<sup>+</sup>
 T<sub>1</sub>
 was 9.5 ms (9.4T, 24°C). The Na<sup>+</sup>
 amounts and spontaneous efflux rate constants were found to be identical within experimental error whether measured by MRR/RR<sub>e</sub>
 or by MRS/SR<sub>e</sub>
. Without RR<sub>e</sub>
, the Na<sup>+</sup>
-loaded yeast cell suspension <sup>23</sup>
Na MR signal exhibited two T<sub>1</sub>
 values, 9.1 (± 0.3) ms and 32.7 (± 2.3) ms, assigned to <sup>23</sup>
Na<sub>i</sub>
<sup>+</sup>
 and <sup>23</sup>
Na<sub>e</sub>
<sup>+</sup>
, respectively. The Na<sub>i</sub>
<sup>+</sup>
 content measured was lower, 0.88 (± 0.06); while Na<sub>e</sub>
<sup>+</sup>
 was higher, 1.43 (± 0.12) compared with MRS/SR<sub>e</sub>
 measures on the same samples. However, the measured efflux rate constant was identical. T<sub>1</sub>
 MRR potentially may be used for Na<sub>i</sub>
<sup>+</sup>
 determination <italic>in vivo</italic>
 and Na<sup>+</sup>
 flux measurements; with RR<sub>e</sub>
 for animal studies and without RR<sub>e</sub>
 for humans.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <pmc-dir>properties manuscript</pmc-dir>
  <front><journal-meta><journal-id journal-id-type="nlm-journal-id">9707935</journal-id>
<journal-id journal-id-type="pubmed-jr-id">20597</journal-id>
<journal-id journal-id-type="nlm-ta">J Magn Reson</journal-id>
<journal-title>Journal of magnetic resonance (San Diego, Calif. : 1997)</journal-title>
<issn pub-type="ppub">1090-7807</issn>
<issn pub-type="epub">1096-0856</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">20430659</article-id>
<article-id pub-id-type="pmc">2885488</article-id>
<article-id pub-id-type="doi">10.1016/j.jmr.2010.03.018</article-id>
<article-id pub-id-type="manuscript">NIHMS193857</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Discrimination of Intra- and Extracellular <sup>23</sup>
Na<sup>+</sup>
 Signals in Yeast Cell Suspensions Using Longitudinal Magnetic Resonance Relaxography</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Zhang</surname>
<given-names>Yajie</given-names>
</name>
<xref rid="A1" ref-type="aff">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Poirer-Quinot</surname>
<given-names>Marie</given-names>
</name>
<xref rid="A1" ref-type="aff">1</xref>
<xref rid="FN2" ref-type="author-notes">#</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Springer</surname>
<given-names>Charles S.</given-names>
<suffix>Jr.</suffix>
</name>
<xref rid="A2" ref-type="aff">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Balschi</surname>
<given-names>James A</given-names>
</name>
<xref rid="A1" ref-type="aff">1</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>1</label>
 Physiological NMR Core Laboratory, Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115</aff>
<aff id="A2"><label>2</label>
 Advanced Imaging Research Center, Oregon Health Science University, 3181 SW Sam Jackson Park Road, Mailcode L452, Portland, OR 97239</aff>
<author-notes><corresp id="FN1">Corresponding author: James A. Balschi, Ph.D., 4 Blackfan St., HIM 816, Boston, MA 02115 USA, Phone (617) 525-5040, <email>jbalschi@rics.bwh.harvard.edu</email>
</corresp>
<fn id="FN2" fn-type="present-address"><label>#</label>
<p>Current Address: U2R2M (UMR8081), University of Paris-Sud, Centre National de la Recherche Scientifique (CNRS), Orsay, France.</p>
</fn>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>12</day>
<month>4</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="epub"><day>1</day>
<month>4</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="ppub"><month>7</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>1</day>
<month>7</month>
<year>2011</year>
</pub-date>
<volume>205</volume>
<issue>1</issue>
<fpage>28</fpage>
<lpage>37</lpage>
<abstract><p id="P2">This study tested the ability of MR Relaxography (MRR) to discriminate intra- (Na<sub>i</sub>
<sup>+</sup>
) and extracellular (Na<sub>e</sub>
<sup>+</sup>
) <sup>23</sup>
Na<sup>+</sup>
 signals using their longitudinal relaxation time constant (T<sub>1</sub>
) values. Na<sup>+</sup>
-loaded yeast cell (<italic>Saccharomyces cerevisiae</italic>
) suspensions were investigated. Two types of compartmental <sup>23</sup>
Na<sup>+</sup>
 T<sub>1</sub>
 differences were examined: a selective Na<sub>e</sub>
<sup>+</sup>
 T<sub>1</sub>
 decrease induced by an extracellular relaxation reagent (RR<sub>e</sub>
), GdDOTP<sup>5−</sup>
; and, an intrinsic T<sub>1</sub>
 difference. Parallel studies using the established method of <sup>23</sup>
Na MRS with an extracellular shift reagent (SR<sub>e</sub>
), TmDOTP<sup>5−</sup>
, were used to validate the MRR measurements. With 12.8 mM RR<sub>e</sub>
, the <sup>23</sup>
Na<sub>e</sub>
<sup>+</sup>
 T<sub>1</sub>
 was 2.4 ms and the <sup>23</sup>
Na<sub>i</sub>
<sup>+</sup>
 T<sub>1</sub>
 was 9.5 ms (9.4T, 24°C). The Na<sup>+</sup>
 amounts and spontaneous efflux rate constants were found to be identical within experimental error whether measured by MRR/RR<sub>e</sub>
 or by MRS/SR<sub>e</sub>
. Without RR<sub>e</sub>
, the Na<sup>+</sup>
-loaded yeast cell suspension <sup>23</sup>
Na MR signal exhibited two T<sub>1</sub>
 values, 9.1 (± 0.3) ms and 32.7 (± 2.3) ms, assigned to <sup>23</sup>
Na<sub>i</sub>
<sup>+</sup>
 and <sup>23</sup>
Na<sub>e</sub>
<sup>+</sup>
, respectively. The Na<sub>i</sub>
<sup>+</sup>
 content measured was lower, 0.88 (± 0.06); while Na<sub>e</sub>
<sup>+</sup>
 was higher, 1.43 (± 0.12) compared with MRS/SR<sub>e</sub>
 measures on the same samples. However, the measured efflux rate constant was identical. T<sub>1</sub>
 MRR potentially may be used for Na<sub>i</sub>
<sup>+</sup>
 determination <italic>in vivo</italic>
 and Na<sup>+</sup>
 flux measurements; with RR<sub>e</sub>
 for animal studies and without RR<sub>e</sub>
 for humans.</p>
</abstract>
<kwd-group><kwd><sup>23</sup>
Na MR</kwd>
<kwd>T<sub>1</sub>
 relaxography</kwd>
<kwd>intracellular Na<sup>+</sup>
</kwd>
<kwd>relaxation reagent</kwd>
</kwd-group>
<contract-num rid="HL1">R01 HL078634-04
				||HL</contract-num>
<contract-sponsor id="HL1">National Heart, Lung, and Blood Institute : NHLBI</contract-sponsor>
</article-meta>
</front>
</pmc>
</record>

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Discrimination of Intra- and Extracellular 23Na+ Signals in Yeast Cell Suspensions Using Longitudinal Magnetic Resonance Relaxography

Discrimination of Intra- and Extracellular 23Na+ Signals in Yeast Cell Suspensions Using Longitudinal Magnetic Resonance Relaxography

Source :

Abstract

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