ThuliumV1, Main, Merge, bibRecord, 001252

Element-tagged immunoassay with ICP-MS detection: evaluation and comparison to conventional immunoassays

Identifieur interne : 001252 ( Main/Merge ); précédent : 001251; suivant : 001253

Element-tagged immunoassay with ICP-MS detection: evaluation and comparison to conventional immunoassays

Auteurs : Eva Razumienko [Canada] ; Olga Ornatsky [Canada] ; Robert Kinach [Canada] ; Michael Milyavsky [Canada] ; Eric Lechman [Canada] ; Vladimir Baranov [Canada] ; Mitchell A. Winnik [Canada] ; Scott D. Tanner [Canada]

Source :

Journal of immunological methods [ 0022-1759 ] ; 2008.

RBID : PMC:2583136

Abstract

We have investigated the possibility of using element-tagged antibodies for protein detection and quantification in microplate format using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), and compared the results to conventional immunoassays, such as Enzyme-Linked Immunosorbent Assay (ELISA) and Western blotting. The technique was further employed to detect low levels and measure DNA-binding activity of transcription factor p53 in leukemia cell lysates through its interaction with immobilized oligonucleotides and recognition by element-tagged antibodies. The advantages of ICP-MS detection for routine performance of immunoassays include increased sensitivity, wide dynamic range, minimal interference from complex matrices, and high throughput. Our approach advances the ICP-MS technology and demonstrates its applicability to proteomic studies through the use of antibodies directly labeled with polymer tags bearing multiple atoms of lanthanides. Development of this novel methodology will enable fast and quantitative identification of multiple analytes in a single well.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2583136

DOI: 10.1016/j.jim.2008.03.011
PubMed: 18456275
PubMed Central: 2583136

Links toward previous steps (curation, corpus...)

to stream Pmc, to step Corpus: 000219
to stream Pmc, to step Curation: 000219
to stream Pmc, to step Checkpoint: 000345
to stream Ncbi, to step Merge: 000234
to stream Ncbi, to step Curation: 000234
to stream Ncbi, to step Checkpoint: 000234

Links to Exploration step

PMC:2583136

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Element-tagged immunoassay with ICP-MS detection: evaluation and comparison to conventional immunoassays</title>
<author><name sortKey="Razumienko, Eva" sort="Razumienko, Eva" uniqKey="Razumienko E" first="Eva" last="Razumienko">Eva Razumienko</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
<country>Canada</country>
<wicri:regionArea>Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
<placeName><settlement type="city">Toronto</settlement>
<region type="state">Ontario</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ornatsky, Olga" sort="Ornatsky, Olga" uniqKey="Ornatsky O" first="Olga" last="Ornatsky">Olga Ornatsky</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
<country>Canada</country>
<wicri:regionArea>Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
<placeName><settlement type="city">Toronto</settlement>
<region type="state">Ontario</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kinach, Robert" sort="Kinach, Robert" uniqKey="Kinach R" first="Robert" last="Kinach">Robert Kinach</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
<country>Canada</country>
<wicri:regionArea>Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
<placeName><settlement type="city">Toronto</settlement>
<region type="state">Ontario</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Milyavsky, Michael" sort="Milyavsky, Michael" uniqKey="Milyavsky M" first="Michael" last="Milyavsky">Michael Milyavsky</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Canada</country>
<wicri:regionArea>Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9</wicri:regionArea>
<wicri:noRegion>Ontario M5G2M9</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lechman, Eric" sort="Lechman, Eric" uniqKey="Lechman E" first="Eric" last="Lechman">Eric Lechman</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Canada</country>
<wicri:regionArea>Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9</wicri:regionArea>
<wicri:noRegion>Ontario M5G2M9</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Baranov, Vladimir" sort="Baranov, Vladimir" uniqKey="Baranov V" first="Vladimir" last="Baranov">Vladimir Baranov</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Canada</country>
<wicri:regionArea>Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9</wicri:regionArea>
<wicri:noRegion>Ontario M5G2M9</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Winnik, Mitchell A" sort="Winnik, Mitchell A" uniqKey="Winnik M" first="Mitchell A." last="Winnik">Mitchell A. Winnik</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
<country>Canada</country>
<wicri:regionArea>Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
<placeName><settlement type="city">Toronto</settlement>
<region type="state">Ontario</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Tanner, Scott D" sort="Tanner, Scott D" uniqKey="Tanner S" first="Scott D." last="Tanner">Scott D. Tanner</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
<country>Canada</country>
<wicri:regionArea>Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
<placeName><settlement type="city">Toronto</settlement>
<region type="state">Ontario</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">18456275</idno>
<idno type="pmc">2583136</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2583136</idno>
<idno type="RBID">PMC:2583136</idno>
<idno type="doi">10.1016/j.jim.2008.03.011</idno>
<date when="2008">2008</date>
<idno type="wicri:Area/Pmc/Corpus">000219</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000219</idno>
<idno type="wicri:Area/Pmc/Curation">000219</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000219</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000345</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000345</idno>
<idno type="wicri:Area/Ncbi/Merge">000234</idno>
<idno type="wicri:Area/Ncbi/Curation">000234</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000234</idno>
<idno type="wicri:doubleKey">0022-1759:2008:Razumienko E:element:tagged:immunoassay</idno>
<idno type="wicri:Area/Main/Merge">001252</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Element-tagged immunoassay with ICP-MS detection: evaluation and comparison to conventional immunoassays</title>
<author><name sortKey="Razumienko, Eva" sort="Razumienko, Eva" uniqKey="Razumienko E" first="Eva" last="Razumienko">Eva Razumienko</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
<country>Canada</country>
<wicri:regionArea>Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
<placeName><settlement type="city">Toronto</settlement>
<region type="state">Ontario</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ornatsky, Olga" sort="Ornatsky, Olga" uniqKey="Ornatsky O" first="Olga" last="Ornatsky">Olga Ornatsky</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
<country>Canada</country>
<wicri:regionArea>Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
<placeName><settlement type="city">Toronto</settlement>
<region type="state">Ontario</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kinach, Robert" sort="Kinach, Robert" uniqKey="Kinach R" first="Robert" last="Kinach">Robert Kinach</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
<country>Canada</country>
<wicri:regionArea>Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
<placeName><settlement type="city">Toronto</settlement>
<region type="state">Ontario</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Milyavsky, Michael" sort="Milyavsky, Michael" uniqKey="Milyavsky M" first="Michael" last="Milyavsky">Michael Milyavsky</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Canada</country>
<wicri:regionArea>Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9</wicri:regionArea>
<wicri:noRegion>Ontario M5G2M9</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lechman, Eric" sort="Lechman, Eric" uniqKey="Lechman E" first="Eric" last="Lechman">Eric Lechman</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Canada</country>
<wicri:regionArea>Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9</wicri:regionArea>
<wicri:noRegion>Ontario M5G2M9</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Baranov, Vladimir" sort="Baranov, Vladimir" uniqKey="Baranov V" first="Vladimir" last="Baranov">Vladimir Baranov</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Canada</country>
<wicri:regionArea>Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9</wicri:regionArea>
<wicri:noRegion>Ontario M5G2M9</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Winnik, Mitchell A" sort="Winnik, Mitchell A" uniqKey="Winnik M" first="Mitchell A." last="Winnik">Mitchell A. Winnik</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
<country>Canada</country>
<wicri:regionArea>Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
<placeName><settlement type="city">Toronto</settlement>
<region type="state">Ontario</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Tanner, Scott D" sort="Tanner, Scott D" uniqKey="Tanner S" first="Scott D." last="Tanner">Scott D. Tanner</name>
<affiliation wicri:level="4"><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
<country>Canada</country>
<wicri:regionArea>Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
<placeName><settlement type="city">Toronto</settlement>
<region type="state">Ontario</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of immunological methods</title>
<idno type="ISSN">0022-1759</idno>
<imprint><date when="2008">2008</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">We have investigated the possibility of using element-tagged antibodies for protein detection and quantification in microplate format using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), and compared the results to conventional immunoassays, such as Enzyme-Linked Immunosorbent Assay (ELISA) and Western blotting. The technique was further employed to detect low levels and measure DNA-binding activity of transcription factor p53 in leukemia cell lysates through its interaction with immobilized oligonucleotides and recognition by element-tagged antibodies. The advantages of ICP-MS detection for routine performance of immunoassays include increased sensitivity, wide dynamic range, minimal interference from complex matrices, and high throughput. Our approach advances the ICP-MS technology and demonstrates its applicability to proteomic studies through the use of antibodies directly labeled with polymer tags bearing multiple atoms of lanthanides. Development of this novel methodology will enable fast and quantitative identification of multiple analytes in a single well.</p>
</div>
</front>
</TEI>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/ThuliumV1/Data/Main/Merge

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001252 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 001252 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Terre
   |area=    ThuliumV1
   |flux=    Main
   |étape=   Merge
   |type=    RBID
   |clé=     PMC:2583136
   |texte=   Element-tagged immunoassay with ICP-MS detection: evaluation and comparison to conventional immunoassays
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i   -Sk "pubmed:18456275" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd   \
       | NlmPubMed2Wicri -a ThuliumV1

This area was generated with Dilib version V0.6.21.
Data generation: Thu May 12 08:27:09 2016. Site generation: Thu Mar 7 22:33:44 2024

	Serveur d'exploration sur le thulium
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le thulium

Element-tagged immunoassay with ICP-MS detection: evaluation and comparison to conventional immunoassays

Element-tagged immunoassay with ICP-MS detection: evaluation and comparison to conventional immunoassays

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki