Detection of Cell and Tissue Surface Antigens Using Up-Converting Phosphors: A New Reporter Technology
Identifieur interne : 002582 ( Main/Exploration ); précédent : 002581; suivant : 002583Detection of Cell and Tissue Surface Antigens Using Up-Converting Phosphors: A New Reporter Technology
Auteurs : H. J. M. A. A. Zijlmans [Pays-Bas] ; J. Bonnet [Pays-Bas] ; J. Burton [États-Unis] ; K. Kardos [États-Unis] ; T. Vail [États-Unis] ; R. S. Niedbala [États-Unis] ; H. J. Tanke [Pays-Bas]Source :
- Analytical Biochemistry [ 0003-2697 ] ; 1999.
Descripteurs français
- KwdFr :
- Animaux, Anticorps, Antigène spécifique de la prostate (analyse), Antigènes CD4 (analyse), Antigènes de surface (analyse), Avidine, Erbium, Humains, Immunohistochimie (), Lymphocytes (immunologie), Mesures de luminescence, Microscopie de fluorescence (), Microscopie de fluorescence (instrumentation), Mâle, Prostate (immunologie), Rayons infrarouges, Thulium, Yttrium.
- MESH :
- analyse : Antigène spécifique de la prostate, Antigènes CD4, Antigènes de surface.
- immunologie : Lymphocytes, Prostate.
- Animaux, Anticorps, Avidine, Erbium, Humains, Immunohistochimie, Mesures de luminescence, Microscopie de fluorescence, Mâle, Rayons infrarouges, Thulium, Yttrium.
English descriptors
- KwdEn :
- Academic press, Animals, Antibodies, Antigens, CD4 (analysis), Antigens, Surface (analysis), Assay, Avidin, Avidinylated fitc, Biological specimens, Biotinylated, Biotinylated antibodies, Biotinylated goat, Bovine serum albumin, Demineralized water, Detection sensitivity, Diagnostic pathology, Erbium, Excitation, Excitation light, Genomic research, Human lymphocytes, Human prostate tissue, Humans, Immunoenzymatic staining, Immunohistochemistry (methods), Immunoreactive molecules, Infrared Rays, Infrared light, Luminescence, Luminescent Measurements, Lymphocytes (immunology), Male, Membrane staining, Microscopy, Fluorescence (instrumentation), Microscopy, Fluorescence (methods), Molecular biology, Mononuclear cells, More photons, Neutravidin, Normal goat serum, Nucleic acids, Phosphor, Phosphor luminescence, Phosphor materials, Phosphor particles, Phosphor suspension, Phosphor technology, Photon, Primary antibodies, Prostate (immunology), Prostate tissue, Prostate-Specific Antigen (analysis), Reporter technology, Room temperature, Same image, Sensitive detection, Simultaneous detection, Supernatant, Target molecules, Thulium, Tissue sections, Visible light, Xenon lamp, Ytterbium, Yttrium, autofluorescence, bioassays, diagnostic pathology, infrared light, luminescence, phosphors, up-conversion.
- MESH :
- chemical , analysis : Antigens, CD4, Antigens, Surface, Prostate-Specific Antigen.
- immunology : Lymphocytes, Prostate.
- instrumentation : Microscopy, Fluorescence.
- methods : Immunohistochemistry, Microscopy, Fluorescence.
- Teeft :
- Academic press, Animals, Antibodies, Assay, Avidin, Avidinylated fitc, Biological specimens, Biotinylated, Biotinylated antibodies, Biotinylated goat, Bovine serum albumin, Demineralized water, Detection sensitivity, Diagnostic pathology, Erbium, Excitation, Excitation light, Genomic research, Human lymphocytes, Human prostate tissue, Humans, Immunoenzymatic staining, Immunoreactive molecules, Infrared Rays, Infrared light, Luminescence, Luminescent Measurements, Male, Membrane staining, Molecular biology, Mononuclear cells, More photons, Neutravidin, Normal goat serum, Nucleic acids, Phosphor, Phosphor luminescence, Phosphor materials, Phosphor particles, Phosphor suspension, Phosphor technology, Photon, Primary antibodies, Prostate tissue, Reporter technology, Room temperature, Same image, Sensitive detection, Simultaneous detection, Supernatant, Target molecules, Thulium, Tissue sections, Visible light, Xenon lamp, Ytterbium, Yttrium.
Abstract
Abstract: A novel luminescent reporter for the sensitive detection of antigens in tissue sections or on cell membranes is described. It consists of submicron-size phosphor crystals (0.2–0.4 μm), which are surface labeled with avidin or antibodies and capable of binding specifically to antigens on intact cells or in tissue sections. These phosphor reporters exhibit two-photon, anti-Stokes luminescence by up-converting infrared to visible light and are named Up-converting Phosphor Technology (UPT). They typically consist of yttriumoxysulfides doped with two different lanthanides exhibiting photostable, strong emission in the visible (blue, green, and red) upon excitation in the infrared. This report describes the conjugation of phosphor particles to NeutrAvidin with the subsequent use of this conjugate in a model system consisting of prostate-specific antigen in tissue sections and the CD4 membrane antigen on human lymphocytes. An epi-illumination fluorescence microscope was adapted to provide near-IR excitation using a xenon lamp for visualization of the visible emission. Advantages of UPT are (i) permanent, strong, anti-Stokes emission of discrete wavelengths; (ii) unmatched contrast in biological specimens due to the absence of autofluorescence upon excitation with IR light; (iii) simultaneous detection of multiple target analytes; and (iv) low-cost microscope modifications. The new methodology has not only high potential value in diagnostic pathology as described here, but may offer advantages for the detection of proteins or nucleic acids when applied to molecular biology, genomic research, virology, and microbiology.
Url:
DOI: 10.1006/abio.1998.2965
Affiliations:
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Le document en format XML
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<term>Antigens, Surface (analysis)</term>
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<term>Avidinylated fitc</term>
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<term>Excitation light</term>
<term>Genomic research</term>
<term>Human lymphocytes</term>
<term>Human prostate tissue</term>
<term>Humans</term>
<term>Immunoenzymatic staining</term>
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<term>Biological specimens</term>
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<term>Biotinylated antibodies</term>
<term>Biotinylated goat</term>
<term>Bovine serum albumin</term>
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<term>Excitation</term>
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<term>Human lymphocytes</term>
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<term>Immunoenzymatic staining</term>
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<term>Phosphor technology</term>
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<term>Visible light</term>
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<term>Yttrium</term>
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<term>Anticorps</term>
<term>Avidine</term>
<term>Erbium</term>
<term>Humains</term>
<term>Immunohistochimie</term>
<term>Mesures de luminescence</term>
<term>Microscopie de fluorescence</term>
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<front><div type="abstract" xml:lang="en">Abstract: A novel luminescent reporter for the sensitive detection of antigens in tissue sections or on cell membranes is described. It consists of submicron-size phosphor crystals (0.2–0.4 μm), which are surface labeled with avidin or antibodies and capable of binding specifically to antigens on intact cells or in tissue sections. These phosphor reporters exhibit two-photon, anti-Stokes luminescence by up-converting infrared to visible light and are named Up-converting Phosphor Technology (UPT). They typically consist of yttriumoxysulfides doped with two different lanthanides exhibiting photostable, strong emission in the visible (blue, green, and red) upon excitation in the infrared. This report describes the conjugation of phosphor particles to NeutrAvidin with the subsequent use of this conjugate in a model system consisting of prostate-specific antigen in tissue sections and the CD4 membrane antigen on human lymphocytes. An epi-illumination fluorescence microscope was adapted to provide near-IR excitation using a xenon lamp for visualization of the visible emission. Advantages of UPT are (i) permanent, strong, anti-Stokes emission of discrete wavelengths; (ii) unmatched contrast in biological specimens due to the absence of autofluorescence upon excitation with IR light; (iii) simultaneous detection of multiple target analytes; and (iv) low-cost microscope modifications. The new methodology has not only high potential value in diagnostic pathology as described here, but may offer advantages for the detection of proteins or nucleic acids when applied to molecular biology, genomic research, virology, and microbiology.</div>
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