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Detection of Cell and Tissue Surface Antigens Using Up-Converting Phosphors: A New Reporter Technology

Identifieur interne : 000C45 ( Istex/Checkpoint ); précédent : 000C44; suivant : 000C46

Detection of Cell and Tissue Surface Antigens Using Up-Converting Phosphors: A New Reporter Technology

Auteurs : H. J. M. A. A. Zijlmans [Pays-Bas] ; J. Bonnet [Pays-Bas] ; J. Burton [États-Unis] ; K. Kardos [États-Unis] ; T. Vail [États-Unis] ; R. S. Niedbala [États-Unis] ; H. J. Tanke [Pays-Bas]

Source :

Analytical Biochemistry [ 0003-2697 ] ; 1999.

RBID : ISTEX:A0AD45CCB5AE80AAB8CD388D210036AAEBEB320E

English descriptors

Abstract

Abstract: A novel luminescent reporter for the sensitive detection of antigens in tissue sections or on cell membranes is described. It consists of submicron-size phosphor crystals (0.2–0.4 μm), which are surface labeled with avidin or antibodies and capable of binding specifically to antigens on intact cells or in tissue sections. These phosphor reporters exhibit two-photon, anti-Stokes luminescence by up-converting infrared to visible light and are named Up-converting Phosphor Technology (UPT). They typically consist of yttriumoxysulfides doped with two different lanthanides exhibiting photostable, strong emission in the visible (blue, green, and red) upon excitation in the infrared. This report describes the conjugation of phosphor particles to NeutrAvidin with the subsequent use of this conjugate in a model system consisting of prostate-specific antigen in tissue sections and the CD4 membrane antigen on human lymphocytes. An epi-illumination fluorescence microscope was adapted to provide near-IR excitation using a xenon lamp for visualization of the visible emission. Advantages of UPT are (i) permanent, strong, anti-Stokes emission of discrete wavelengths; (ii) unmatched contrast in biological specimens due to the absence of autofluorescence upon excitation with IR light; (iii) simultaneous detection of multiple target analytes; and (iv) low-cost microscope modifications. The new methodology has not only high potential value in diagnostic pathology as described here, but may offer advantages for the detection of proteins or nucleic acids when applied to molecular biology, genomic research, virology, and microbiology.

Url:

https://api.istex.fr/document/A0AD45CCB5AE80AAB8CD388D210036AAEBEB320E/fulltext/pdf

DOI: 10.1006/abio.1998.2965

Affiliations:

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Le document en format XML

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<author><name sortKey="Bonnet, J" sort="Bonnet, J" uniqKey="Bonnet J" first="J." last="Bonnet">J. Bonnet</name>
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<term>Biological specimens</term>
<term>Biotinylated</term>
<term>Biotinylated antibodies</term>
<term>Biotinylated goat</term>
<term>Bovine serum albumin</term>
<term>Demineralized water</term>
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<term>Sensitive detection</term>
<term>Simultaneous detection</term>
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<term>Immunoreactive molecules</term>
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<front><div type="abstract" xml:lang="en">Abstract: A novel luminescent reporter for the sensitive detection of antigens in tissue sections or on cell membranes is described. It consists of submicron-size phosphor crystals (0.2–0.4 μm), which are surface labeled with avidin or antibodies and capable of binding specifically to antigens on intact cells or in tissue sections. These phosphor reporters exhibit two-photon, anti-Stokes luminescence by up-converting infrared to visible light and are named Up-converting Phosphor Technology (UPT). They typically consist of yttriumoxysulfides doped with two different lanthanides exhibiting photostable, strong emission in the visible (blue, green, and red) upon excitation in the infrared. This report describes the conjugation of phosphor particles to NeutrAvidin with the subsequent use of this conjugate in a model system consisting of prostate-specific antigen in tissue sections and the CD4 membrane antigen on human lymphocytes. An epi-illumination fluorescence microscope was adapted to provide near-IR excitation using a xenon lamp for visualization of the visible emission. Advantages of UPT are (i) permanent, strong, anti-Stokes emission of discrete wavelengths; (ii) unmatched contrast in biological specimens due to the absence of autofluorescence upon excitation with IR light; (iii) simultaneous detection of multiple target analytes; and (iv) low-cost microscope modifications. The new methodology has not only high potential value in diagnostic pathology as described here, but may offer advantages for the detection of proteins or nucleic acids when applied to molecular biology, genomic research, virology, and microbiology.</div>
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Detection of Cell and Tissue Surface Antigens Using Up-Converting Phosphors: A New Reporter Technology

Detection of Cell and Tissue Surface Antigens Using Up-Converting Phosphors: A New Reporter Technology

Source :

English descriptors

Abstract

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Links to Exploration step

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