7Li nuclear magnetic resonance study for the determination of Li+ properties in neuroblastoma SH-SY5Y cells.
Identifieur interne : 002681 ( Main/Exploration ); précédent : 002680; suivant : 0026827Li nuclear magnetic resonance study for the determination of Li+ properties in neuroblastoma SH-SY5Y cells.
Auteurs : J. Nikolakopoulos [États-Unis] ; C. Zachariah ; D. Mota De Freitas ; E B Stubbs ; R. Ramasamy ; M C Castro ; C F GeraldesSource :
- Journal of neurochemistry [ 0022-3042 ] ; 1998.
Descripteurs français
- KwdFr :
- Acide 4,4'-diisothiocyano-stilbène-2,2'-disulfonique (pharmacologie), Cellules cancéreuses en culture, Humains, Isotopes, Liquide intracellulaire (), Liquide intracellulaire (métabolisme), Lithium (), Lithium (métabolisme), Lithium (pharmacologie), Neuroblastome (), Neuroblastome (anatomopathologie), Neuroblastome (métabolisme), Ouabaïne (pharmacologie), Perfusion, Phlorétine (pharmacologie), Spectroscopie par résonance magnétique, Survie cellulaire (), Transport des ions (), Vératridine (pharmacologie).
- MESH :
- anatomopathologie : Neuroblastome.
- métabolisme : Liquide intracellulaire, Lithium, Neuroblastome.
- pharmacologie : Acide 4,4'-diisothiocyano-stilbène-2,2'-disulfonique, Lithium, Ouabaïne, Phlorétine, Vératridine.
- Cellules cancéreuses en culture, Humains, Isotopes, Liquide intracellulaire, Lithium, Neuroblastome, Perfusion, Spectroscopie par résonance magnétique, Survie cellulaire, Transport des ions.
English descriptors
- KwdEn :
- 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid (pharmacology), Cell Survival (drug effects), Humans, Intracellular Fluid (chemistry), Intracellular Fluid (metabolism), Ion Transport (drug effects), Isotopes, Lithium (chemistry), Lithium (metabolism), Lithium (pharmacology), Magnetic Resonance Spectroscopy, Neuroblastoma (chemistry), Neuroblastoma (metabolism), Neuroblastoma (pathology), Ouabain (pharmacology), Perfusion, Phloretin (pharmacology), Tumor Cells, Cultured, Veratridine (pharmacology).
- MESH :
- chemical , chemistry : Lithium.
- chemical , metabolism : Lithium.
- chemical , pharmacology : 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid, Lithium, Ouabain, Phloretin, Veratridine.
- chemistry : Intracellular Fluid, Neuroblastoma.
- drug effects : Cell Survival, Ion Transport.
- metabolism : Intracellular Fluid, Neuroblastoma.
- pathology : Neuroblastoma.
- Humans, Isotopes, Magnetic Resonance Spectroscopy, Perfusion, Tumor Cells, Cultured.
Abstract
Lithium has been used clinically in the treatment of manic depression. However, its pharmacologic mode of action remains unclear. Characteristics of Li+ interactions in red blood cells (RBCs) have been identified. We investigated Li+ interactions on human neuroblastoma SH-SY5Y cells by developing a novel 7Li NMR method that provided a clear estimation of the intra- and extracellular amounts of Li+ in the presence of the shift reagent thulium-1,4,7,10-tetrazacyclododecane-N,N',N'',N'''-tetramethylene phosphonate (HTmDOTP4-). The first-order rate constants of Li+ influx and efflux for perfused, agarose-embedded SH-SY5Y cells in the presence of 3 mM HTmDOTP4- were 0.055 +/- 0.006 (n = 4) and -0.025 +/- 0.006 min(-1) (n = 3), respectively. Significant increases in the rate constants of Li+ influx and efflux in the presence of 0.05 mM veratridine indicated the presence of Na+ channel-mediated Li+ transport in SH-SY5Y cells. 7Li NMR relaxation measurements showed that Li+ is immobilized more in human neuroblastoma SH-SY5Y cells than in human RBCs.
PubMed: 9751202
Affiliations:
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Le document en format XML
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Ramasamy</name></author><author><name sortKey="Castro, M C" sort="Castro, M C" uniqKey="Castro M" first="M C" last="Castro">M C Castro</name></author><author><name sortKey="Geraldes, C F" sort="Geraldes, C F" uniqKey="Geraldes C" first="C F" last="Geraldes">C F Geraldes</name></author></analytic><series><title level="j">Journal of neurochemistry</title><idno type="ISSN">0022-3042</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid (pharmacology)</term><term>Cell Survival (drug effects)</term><term>Humans</term><term>Intracellular Fluid (chemistry)</term><term>Intracellular Fluid (metabolism)</term><term>Ion Transport (drug effects)</term><term>Isotopes</term><term>Lithium (chemistry)</term><term>Lithium (metabolism)</term><term>Lithium (pharmacology)</term><term>Magnetic Resonance Spectroscopy</term><term>Neuroblastoma (chemistry)</term><term>Neuroblastoma (metabolism)</term><term>Neuroblastoma (pathology)</term><term>Ouabain (pharmacology)</term><term>Perfusion</term><term>Phloretin (pharmacology)</term><term>Tumor Cells, Cultured</term><term>Veratridine (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide 4,4'-diisothiocyano-stilbène-2,2'-disulfonique (pharmacologie)</term><term>Cellules cancéreuses en culture</term><term>Humains</term><term>Isotopes</term><term>Liquide intracellulaire ()</term><term>Liquide intracellulaire (métabolisme)</term><term>Lithium ()</term><term>Lithium (métabolisme)</term><term>Lithium (pharmacologie)</term><term>Neuroblastome ()</term><term>Neuroblastome (anatomopathologie)</term><term>Neuroblastome (métabolisme)</term><term>Ouabaïne (pharmacologie)</term><term>Perfusion</term><term>Phlorétine (pharmacologie)</term><term>Spectroscopie par résonance magnétique</term><term>Survie cellulaire ()</term><term>Transport des ions ()</term><term>Vératridine (pharmacologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lithium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lithium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid</term><term>Lithium</term><term>Ouabain</term><term>Phloretin</term><term>Veratridine</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Neuroblastome</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Intracellular Fluid</term><term>Neuroblastoma</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Survival</term><term>Ion Transport</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Intracellular Fluid</term><term>Neuroblastoma</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Liquide intracellulaire</term><term>Lithium</term><term>Neuroblastome</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Neuroblastoma</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acide 4,4'-diisothiocyano-stilbène-2,2'-disulfonique</term><term>Lithium</term><term>Ouabaïne</term><term>Phlorétine</term><term>Vératridine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Isotopes</term><term>Magnetic Resonance Spectroscopy</term><term>Perfusion</term><term>Tumor Cells, Cultured</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cancéreuses en culture</term><term>Humains</term><term>Isotopes</term><term>Liquide intracellulaire</term><term>Lithium</term><term>Neuroblastome</term><term>Perfusion</term><term>Spectroscopie par résonance magnétique</term><term>Survie cellulaire</term><term>Transport des ions</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lithium has been used clinically in the treatment of manic depression. However, its pharmacologic mode of action remains unclear. Characteristics of Li+ interactions in red blood cells (RBCs) have been identified. We investigated Li+ interactions on human neuroblastoma SH-SY5Y cells by developing a novel 7Li NMR method that provided a clear estimation of the intra- and extracellular amounts of Li+ in the presence of the shift reagent thulium-1,4,7,10-tetrazacyclododecane-N,N',N'',N'''-tetramethylene phosphonate (HTmDOTP4-). The first-order rate constants of Li+ influx and efflux for perfused, agarose-embedded SH-SY5Y cells in the presence of 3 mM HTmDOTP4- were 0.055 +/- 0.006 (n = 4) and -0.025 +/- 0.006 min(-1) (n = 3), respectively. Significant increases in the rate constants of Li+ influx and efflux in the presence of 0.05 mM veratridine indicated the presence of Na+ channel-mediated Li+ transport in SH-SY5Y cells. 7Li NMR relaxation measurements showed that Li+ is immobilized more in human neuroblastoma SH-SY5Y cells than in human RBCs.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Castro, M C" sort="Castro, M C" uniqKey="Castro M" first="M C" last="Castro">M C Castro</name><name sortKey="Geraldes, C F" sort="Geraldes, C F" uniqKey="Geraldes C" first="C F" last="Geraldes">C F Geraldes</name><name sortKey="Mota De Freitas, D" sort="Mota De Freitas, D" uniqKey="Mota De Freitas D" first="D" last="Mota De Freitas">D. Mota De Freitas</name><name sortKey="Ramasamy, R" sort="Ramasamy, R" uniqKey="Ramasamy R" first="R" last="Ramasamy">R. Ramasamy</name><name sortKey="Stubbs, E B" sort="Stubbs, E B" uniqKey="Stubbs E" first="E B" last="Stubbs">E B Stubbs</name><name sortKey="Zachariah, C" sort="Zachariah, C" uniqKey="Zachariah C" first="C" last="Zachariah">C. Zachariah</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Nikolakopoulos, J" sort="Nikolakopoulos, J" uniqKey="Nikolakopoulos J" first="J" last="Nikolakopoulos">J. Nikolakopoulos</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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