An Arabidopsis chloroplast RNA-binding protein gene encodes multiple mRNAs with different 5' ends.
Identifieur interne : 003129 ( Main/Exploration ); précédent : 003128; suivant : 003130An Arabidopsis chloroplast RNA-binding protein gene encodes multiple mRNAs with different 5' ends.
Auteurs : S H Cheng ; K. Cline ; A J DelisleSource :
- Plant Physiology [ 0032-0889 ] ; 1994.
Abstract
An Arabidopsis cDNA (Atrbp33) encoding a nuclear-encoded chloroplast RNA-binding protein (RBP) has been isolated (A.J. DeLisle [1993] Plant Physiol 102: 313-314). ATRBP33 shares global structural homology with all known chloroplast RBPs: a chloroplast transit peptide in the amino terminus, followed by a unique acidic domain and a tandem pair of ribonucleoprotein consensus sequence-type RNA-binding domains in the carboxyl end. In vitro translation products of Atrbp33 were found to be imported into chloroplasts, suggesting that ATRBP33 is localized in chloroplasts. The expression of Atrbp33 was higher in chloroplast-containing organs than in nonchloroplast-containing organs. Furthermore, Atrbp33 was expressed in a light-dependent manner. These features are consistent with its postulated role in posttranscriptional control of chloroplast genes. Northern analyses and RNase protection assays showed that as many as nine messages are encoded by the single Atrbp33 gene. Sequence analysis of the cDNAs indicated that some of the transcripts have truncated 5' ends. Most interestingly, the multiple mRNAs potentially encode different polypeptides, one of which lacks a chloroplast transit peptide and acidic domain and contains only one intact RNA-binding domain. Unlike the chloroplast-localized ATRBP33, the truncated polypeptide may function in other cellular compartments.
Url:
PubMed: 7972518
PubMed Central: 159528
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000175
- to stream Pmc, to step Curation: 000175
- to stream Pmc, to step Checkpoint: 000397
- to stream Ncbi, to step Merge: 000C20
- to stream Ncbi, to step Curation: 000C20
- to stream Ncbi, to step Checkpoint: 000C20
- to stream Main, to step Merge: 003386
- to stream Main, to step Curation: 003129
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An Arabidopsis chloroplast RNA-binding protein gene encodes multiple mRNAs with different 5' ends.</title><author><name sortKey="Cheng, S H" sort="Cheng, S H" uniqKey="Cheng S" first="S H" last="Cheng">S H Cheng</name></author><author><name sortKey="Cline, K" sort="Cline, K" uniqKey="Cline K" first="K" last="Cline">K. Cline</name></author><author><name sortKey="Delisle, A J" sort="Delisle, A J" uniqKey="Delisle A" first="A J" last="Delisle">A J Delisle</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">7972518</idno><idno type="pmc">159528</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC159528</idno><idno type="RBID">PMC:159528</idno><date when="1994">1994</date><idno type="wicri:Area/Pmc/Corpus">000175</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000175</idno><idno type="wicri:Area/Pmc/Curation">000175</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000175</idno><idno type="wicri:Area/Pmc/Checkpoint">000397</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000397</idno><idno type="wicri:Area/Ncbi/Merge">000C20</idno><idno type="wicri:Area/Ncbi/Curation">000C20</idno><idno type="wicri:Area/Ncbi/Checkpoint">000C20</idno><idno type="wicri:doubleKey">0032-0889:1994:Cheng S:an:arabidopsis:chloroplast</idno><idno type="wicri:Area/Main/Merge">003386</idno><idno type="wicri:Area/Main/Curation">003129</idno><idno type="wicri:Area/Main/Exploration">003129</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">An Arabidopsis chloroplast RNA-binding protein gene encodes multiple mRNAs with different 5' ends.</title><author><name sortKey="Cheng, S H" sort="Cheng, S H" uniqKey="Cheng S" first="S H" last="Cheng">S H Cheng</name></author><author><name sortKey="Cline, K" sort="Cline, K" uniqKey="Cline K" first="K" last="Cline">K. Cline</name></author><author><name sortKey="Delisle, A J" sort="Delisle, A J" uniqKey="Delisle A" first="A J" last="Delisle">A J Delisle</name></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="1994">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>An Arabidopsis cDNA (Atrbp33) encoding a nuclear-encoded chloroplast RNA-binding protein (RBP) has been isolated (A.J. DeLisle [1993] Plant Physiol 102: 313-314). ATRBP33 shares global structural homology with all known chloroplast RBPs: a chloroplast transit peptide in the amino terminus, followed by a unique acidic domain and a tandem pair of ribonucleoprotein consensus sequence-type RNA-binding domains in the carboxyl end. In vitro translation products of Atrbp33 were found to be imported into chloroplasts, suggesting that ATRBP33 is localized in chloroplasts. The expression of Atrbp33 was higher in chloroplast-containing organs than in nonchloroplast-containing organs. Furthermore, Atrbp33 was expressed in a light-dependent manner. These features are consistent with its postulated role in posttranscriptional control of chloroplast genes. Northern analyses and RNase protection assays showed that as many as nine messages are encoded by the single Atrbp33 gene. Sequence analysis of the cDNAs indicated that some of the transcripts have truncated 5' ends. Most interestingly, the multiple mRNAs potentially encode different polypeptides, one of which lacks a chloroplast transit peptide and acidic domain and contains only one intact RNA-binding domain. Unlike the chloroplast-localized ATRBP33, the truncated polypeptide may function in other cellular compartments.</p></div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Cheng, S H" sort="Cheng, S H" uniqKey="Cheng S" first="S H" last="Cheng">S H Cheng</name><name sortKey="Cline, K" sort="Cline, K" uniqKey="Cline K" first="K" last="Cline">K. Cline</name><name sortKey="Delisle, A J" sort="Delisle, A J" uniqKey="Delisle A" first="A J" last="Delisle">A J Delisle</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/ThuliumV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003129 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003129 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Terre
|area= ThuliumV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:159528
|texte= An Arabidopsis chloroplast RNA-binding protein gene encodes multiple mRNAs with different 5' ends.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:7972518" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ThuliumV1
| This area was generated with Dilib version V0.6.21. |  |