Alteration of the Amount of the Chloroplast Phosphate Translocator in Transgenic Tobacco Affects the Distribution of Assimilate between Starch and Sugar.
Identifieur interne : 003130 ( Main/Exploration ); précédent : 003129; suivant : 003131Alteration of the Amount of the Chloroplast Phosphate Translocator in Transgenic Tobacco Affects the Distribution of Assimilate between Starch and Sugar.
Auteurs : S. A. Barnes ; J. S. Knight ; J. C. GraySource :
- Plant Physiology [ 0032-0889 ] ; 1994.
Abstract
Tobacco plants (Nicotiana tabacum L.) transformed with sense and antisense constructs of a cDNA encoding the tobacco phosphate-triose phosphate-3-phosphoglycerate translocator (phosphate translocator) were shown to contain altered amounts of phosphate translocator mRNA and protein. Phosphate translocator activity in intact chloroplasts isolated from transformed plants showed a 15-fold variation, from 20% of the wild-type activity in antisense transformants to 300% of the wild-type activity in sense transformants. However, the maximal rates of photosynthesis and the rates of photosynthetic carbon assimilation in ambient CO2 showed no consistent differences between transformants. Starch content was decreased by 20% and total soluble sugars were increased by 20% in leaves of antisense transformants compared to sense transformants. The 40% decrease in the ratio of starch to total soluble sugars in antisense transformants relative to sense transformants indicates that distribution of assimilate between starch and sugar had been altered. However, the amount of sucrose in the leaves was unchanged. The changes in total soluble sugars were accounted for completely by changes in glucose and fructose, suggesting the existence of a homeostatic mechanism for maintaining sucrose concentrations in the leaves at the expense of glucose and fructose.
Url:
PubMed: 12232394
PubMed Central: 159639
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000173
- to stream Pmc, to step Curation: 000173
- to stream Pmc, to step Checkpoint: 000398
- to stream Ncbi, to step Merge: 000036
- to stream Ncbi, to step Curation: 000036
- to stream Ncbi, to step Checkpoint: 000036
- to stream Main, to step Merge: 003387
- to stream Main, to step Curation: 003130
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Alteration of the Amount of the Chloroplast Phosphate Translocator in Transgenic Tobacco Affects the Distribution of Assimilate between Starch and Sugar.</title>
<author><name sortKey="Barnes, S A" sort="Barnes, S A" uniqKey="Barnes S" first="S. A." last="Barnes">S. A. Barnes</name>
</author>
<author><name sortKey="Knight, J S" sort="Knight, J S" uniqKey="Knight J" first="J. S." last="Knight">J. S. Knight</name>
</author>
<author><name sortKey="Gray, J C" sort="Gray, J C" uniqKey="Gray J" first="J. C." last="Gray">J. C. Gray</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">12232394</idno>
<idno type="pmc">159639</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC159639</idno>
<idno type="RBID">PMC:159639</idno>
<date when="1994">1994</date>
<idno type="wicri:Area/Pmc/Corpus">000173</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000173</idno>
<idno type="wicri:Area/Pmc/Curation">000173</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000173</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000398</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000398</idno>
<idno type="wicri:Area/Ncbi/Merge">000036</idno>
<idno type="wicri:Area/Ncbi/Curation">000036</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000036</idno>
<idno type="wicri:doubleKey">0032-0889:1994:Barnes S:alteration:of:the</idno>
<idno type="wicri:Area/Main/Merge">003387</idno>
<idno type="wicri:Area/Main/Curation">003130</idno>
<idno type="wicri:Area/Main/Exploration">003130</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Alteration of the Amount of the Chloroplast Phosphate Translocator in Transgenic Tobacco Affects the Distribution of Assimilate between Starch and Sugar.</title>
<author><name sortKey="Barnes, S A" sort="Barnes, S A" uniqKey="Barnes S" first="S. A." last="Barnes">S. A. Barnes</name>
</author>
<author><name sortKey="Knight, J S" sort="Knight, J S" uniqKey="Knight J" first="J. S." last="Knight">J. S. Knight</name>
</author>
<author><name sortKey="Gray, J C" sort="Gray, J C" uniqKey="Gray J" first="J. C." last="Gray">J. C. Gray</name>
</author>
</analytic>
<series><title level="j">Plant Physiology</title>
<idno type="ISSN">0032-0889</idno>
<idno type="eISSN">1532-2548</idno>
<imprint><date when="1994">1994</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Tobacco plants (Nicotiana tabacum L.) transformed with sense and antisense constructs of a cDNA encoding the tobacco phosphate-triose phosphate-3-phosphoglycerate translocator (phosphate translocator) were shown to contain altered amounts of phosphate translocator mRNA and protein. Phosphate translocator activity in intact chloroplasts isolated from transformed plants showed a 15-fold variation, from 20% of the wild-type activity in antisense transformants to 300% of the wild-type activity in sense transformants. However, the maximal rates of photosynthesis and the rates of photosynthetic carbon assimilation in ambient CO2 showed no consistent differences between transformants. Starch content was decreased by 20% and total soluble sugars were increased by 20% in leaves of antisense transformants compared to sense transformants. The 40% decrease in the ratio of starch to total soluble sugars in antisense transformants relative to sense transformants indicates that distribution of assimilate between starch and sugar had been altered. However, the amount of sucrose in the leaves was unchanged. The changes in total soluble sugars were accounted for completely by changes in glucose and fructose, suggesting the existence of a homeostatic mechanism for maintaining sucrose concentrations in the leaves at the expense of glucose and fructose.</p>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Barnes, S A" sort="Barnes, S A" uniqKey="Barnes S" first="S. A." last="Barnes">S. A. Barnes</name>
<name sortKey="Gray, J C" sort="Gray, J C" uniqKey="Gray J" first="J. C." last="Gray">J. C. Gray</name>
<name sortKey="Knight, J S" sort="Knight, J S" uniqKey="Knight J" first="J. S." last="Knight">J. S. Knight</name>
</noCountry>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/ThuliumV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003130 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003130 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Terre |area= ThuliumV1 |flux= Main |étape= Exploration |type= RBID |clé= PMC:159639 |texte= Alteration of the Amount of the Chloroplast Phosphate Translocator in Transgenic Tobacco Affects the Distribution of Assimilate between Starch and Sugar. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:12232394" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a ThuliumV1
![]() | This area was generated with Dilib version V0.6.21. | ![]() |