Quantitation of intracellular [Na+] in vivo by using TmDOTP5- as an NMR shift reagent and extracellular marker.
Identifieur interne : 002679 ( Main/Exploration ); précédent : 002678; suivant : 002680Quantitation of intracellular [Na+] in vivo by using TmDOTP5- as an NMR shift reagent and extracellular marker.
Auteurs : P M Winter [États-Unis] ; V. Seshan ; J D Makos ; A D Sherry ; C R Malloy ; N. BansalSource :
- Journal of applied physiology (Bethesda, Md. : 1985) [ 8750-7587 ] ; 1998.
Descripteurs français
- KwdFr :
- Animaux, Calibrage, Composés organiques du phosphore, Composés organométalliques, Espace extracellulaire (métabolisme), Indicateurs et réactifs, Mâle, Radio-isotopes du phosphore, Radio-isotopes du sodium, Rat Sprague-Dawley, Rats, Sodium (métabolisme), Spectrophotométrie atomique, Spectroscopie par résonance magnétique, Thulium.
- MESH :
- métabolisme : Espace extracellulaire, Sodium.
- Animaux, Calibrage, Composés organiques du phosphore, Composés organométalliques, Indicateurs et réactifs, Mâle, Radio-isotopes du phosphore, Radio-isotopes du sodium, Rat Sprague-Dawley, Rats, Spectrophotométrie atomique, Spectroscopie par résonance magnétique, Thulium.
English descriptors
- KwdEn :
- Animals, Calibration, Extracellular Space (metabolism), Indicators and Reagents, Magnetic Resonance Spectroscopy, Male, Organometallic Compounds, Organophosphorus Compounds, Phosphorus Radioisotopes, Rats, Rats, Sprague-Dawley, Sodium (metabolism), Sodium Radioisotopes, Spectrophotometry, Atomic, Thulium.
- MESH :
- chemical , metabolism : Sodium.
- chemical : Indicators and Reagents, Organometallic Compounds, Organophosphorus Compounds, Phosphorus Radioisotopes, Sodium Radioisotopes, Thulium.
- metabolism : Extracellular Space.
- Animals, Calibration, Magnetic Resonance Spectroscopy, Male, Rats, Rats, Sprague-Dawley, Spectrophotometry, Atomic.
Abstract
A method is presented to measure the absolute concentration of intracellular Na+ ([Na+]i) in vivo by using interleaved 23Na- and 31P-nuclear magnetic resonance (NMR) spectroscopy and TmDOTP5- as shift reagent and chemical marker of tissue extracellular space (ECS). The technique was used to determine [Na+]i and relative ECS in livers of control rats (21 +/- 3 and 0.11 +/- 0.02 mM, respectively) and in rats exposed to carbon tetrachloride (103 +/- 29 and 0.23 +/- 0.03 mM, respectively). The NMR measurements were confirmed independently on excised tissue samples by using atomic absorption spectroscopy. The results confirm that TmDOTP5- can be used as a combined cation shift reagent and ECS marker, thereby allowing quantitation of [Na+]i in vivo by NMR.
PubMed: 9804585
Affiliations:
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Le document en format XML
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<term>Indicators and Reagents</term>
<term>Magnetic Resonance Spectroscopy</term>
<term>Male</term>
<term>Organometallic Compounds</term>
<term>Organophosphorus Compounds</term>
<term>Phosphorus Radioisotopes</term>
<term>Rats</term>
<term>Rats, Sprague-Dawley</term>
<term>Sodium (metabolism)</term>
<term>Sodium Radioisotopes</term>
<term>Spectrophotometry, Atomic</term>
<term>Thulium</term>
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<term>Calibrage</term>
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<term>Espace extracellulaire (métabolisme)</term>
<term>Indicateurs et réactifs</term>
<term>Mâle</term>
<term>Radio-isotopes du phosphore</term>
<term>Radio-isotopes du sodium</term>
<term>Rat Sprague-Dawley</term>
<term>Rats</term>
<term>Sodium (métabolisme)</term>
<term>Spectrophotométrie atomique</term>
<term>Spectroscopie par résonance magnétique</term>
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<term>Phosphorus Radioisotopes</term>
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<term>Indicateurs et réactifs</term>
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<term>Radio-isotopes du sodium</term>
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<front><div type="abstract" xml:lang="en">A method is presented to measure the absolute concentration of intracellular Na+ ([Na+]i) in vivo by using interleaved 23Na- and 31P-nuclear magnetic resonance (NMR) spectroscopy and TmDOTP5- as shift reagent and chemical marker of tissue extracellular space (ECS). The technique was used to determine [Na+]i and relative ECS in livers of control rats (21 +/- 3 and 0.11 +/- 0.02 mM, respectively) and in rats exposed to carbon tetrachloride (103 +/- 29 and 0.23 +/- 0.03 mM, respectively). The NMR measurements were confirmed independently on excised tissue samples by using atomic absorption spectroscopy. The results confirm that TmDOTP5- can be used as a combined cation shift reagent and ECS marker, thereby allowing quantitation of [Na+]i in vivo by NMR.</div>
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