Quantitation of intracellular [Na+] in vivo by using TmDOTP5- as an NMR shift reagent and extracellular marker.
Identifieur interne : 002679 ( Main/Exploration ); précédent : 002678; suivant : 002680Quantitation of intracellular [Na+] in vivo by using TmDOTP5- as an NMR shift reagent and extracellular marker.
Auteurs : P M Winter [États-Unis] ; V. Seshan ; J D Makos ; A D Sherry ; C R Malloy ; N. BansalSource :
- Journal of applied physiology (Bethesda, Md. : 1985) [ 8750-7587 ] ; 1998.
Descripteurs français
- KwdFr :
- Animaux, Calibrage, Composés organiques du phosphore, Composés organométalliques, Espace extracellulaire (métabolisme), Indicateurs et réactifs, Mâle, Radio-isotopes du phosphore, Radio-isotopes du sodium, Rat Sprague-Dawley, Rats, Sodium (métabolisme), Spectrophotométrie atomique, Spectroscopie par résonance magnétique, Thulium.
- MESH :
- métabolisme : Espace extracellulaire, Sodium.
- Animaux, Calibrage, Composés organiques du phosphore, Composés organométalliques, Indicateurs et réactifs, Mâle, Radio-isotopes du phosphore, Radio-isotopes du sodium, Rat Sprague-Dawley, Rats, Spectrophotométrie atomique, Spectroscopie par résonance magnétique, Thulium.
English descriptors
- KwdEn :
- Animals, Calibration, Extracellular Space (metabolism), Indicators and Reagents, Magnetic Resonance Spectroscopy, Male, Organometallic Compounds, Organophosphorus Compounds, Phosphorus Radioisotopes, Rats, Rats, Sprague-Dawley, Sodium (metabolism), Sodium Radioisotopes, Spectrophotometry, Atomic, Thulium.
- MESH :
- chemical , metabolism : Sodium.
- chemical : Indicators and Reagents, Organometallic Compounds, Organophosphorus Compounds, Phosphorus Radioisotopes, Sodium Radioisotopes, Thulium.
- metabolism : Extracellular Space.
- Animals, Calibration, Magnetic Resonance Spectroscopy, Male, Rats, Rats, Sprague-Dawley, Spectrophotometry, Atomic.
Abstract
A method is presented to measure the absolute concentration of intracellular Na+ ([Na+]i) in vivo by using interleaved 23Na- and 31P-nuclear magnetic resonance (NMR) spectroscopy and TmDOTP5- as shift reagent and chemical marker of tissue extracellular space (ECS). The technique was used to determine [Na+]i and relative ECS in livers of control rats (21 +/- 3 and 0.11 +/- 0.02 mM, respectively) and in rats exposed to carbon tetrachloride (103 +/- 29 and 0.23 +/- 0.03 mM, respectively). The NMR measurements were confirmed independently on excised tissue samples by using atomic absorption spectroscopy. The results confirm that TmDOTP5- can be used as a combined cation shift reagent and ECS marker, thereby allowing quantitation of [Na+]i in vivo by NMR.
PubMed: 9804585
Affiliations:
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Seshan</name></author><author><name sortKey="Makos, J D" sort="Makos, J D" uniqKey="Makos J" first="J D" last="Makos">J D Makos</name></author><author><name sortKey="Sherry, A D" sort="Sherry, A D" uniqKey="Sherry A" first="A D" last="Sherry">A D Sherry</name></author><author><name sortKey="Malloy, C R" sort="Malloy, C R" uniqKey="Malloy C" first="C R" last="Malloy">C R Malloy</name></author><author><name sortKey="Bansal, N" sort="Bansal, N" uniqKey="Bansal N" first="N" last="Bansal">N. 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Seshan</name></author><author><name sortKey="Makos, J D" sort="Makos, J D" uniqKey="Makos J" first="J D" last="Makos">J D Makos</name></author><author><name sortKey="Sherry, A D" sort="Sherry, A D" uniqKey="Sherry A" first="A D" last="Sherry">A D Sherry</name></author><author><name sortKey="Malloy, C R" sort="Malloy, C R" uniqKey="Malloy C" first="C R" last="Malloy">C R Malloy</name></author><author><name sortKey="Bansal, N" sort="Bansal, N" uniqKey="Bansal N" first="N" last="Bansal">N. Bansal</name></author></analytic><series><title level="j">Journal of applied physiology (Bethesda, Md. : 1985)</title><idno type="ISSN">8750-7587</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Calibration</term><term>Extracellular Space (metabolism)</term><term>Indicators and Reagents</term><term>Magnetic Resonance Spectroscopy</term><term>Male</term><term>Organometallic Compounds</term><term>Organophosphorus Compounds</term><term>Phosphorus Radioisotopes</term><term>Rats</term><term>Rats, Sprague-Dawley</term><term>Sodium (metabolism)</term><term>Sodium Radioisotopes</term><term>Spectrophotometry, Atomic</term><term>Thulium</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Calibrage</term><term>Composés organiques du phosphore</term><term>Composés organométalliques</term><term>Espace extracellulaire (métabolisme)</term><term>Indicateurs et réactifs</term><term>Mâle</term><term>Radio-isotopes du phosphore</term><term>Radio-isotopes du sodium</term><term>Rat Sprague-Dawley</term><term>Rats</term><term>Sodium (métabolisme)</term><term>Spectrophotométrie atomique</term><term>Spectroscopie par résonance magnétique</term><term>Thulium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Sodium</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Indicators and Reagents</term><term>Organometallic Compounds</term><term>Organophosphorus Compounds</term><term>Phosphorus Radioisotopes</term><term>Sodium Radioisotopes</term><term>Thulium</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Extracellular Space</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Espace extracellulaire</term><term>Sodium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Calibration</term><term>Magnetic Resonance Spectroscopy</term><term>Male</term><term>Rats</term><term>Rats, Sprague-Dawley</term><term>Spectrophotometry, Atomic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Calibrage</term><term>Composés organiques du phosphore</term><term>Composés organométalliques</term><term>Indicateurs et réactifs</term><term>Mâle</term><term>Radio-isotopes du phosphore</term><term>Radio-isotopes du sodium</term><term>Rat Sprague-Dawley</term><term>Rats</term><term>Spectrophotométrie atomique</term><term>Spectroscopie par résonance magnétique</term><term>Thulium</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A method is presented to measure the absolute concentration of intracellular Na+ ([Na+]i) in vivo by using interleaved 23Na- and 31P-nuclear magnetic resonance (NMR) spectroscopy and TmDOTP5- as shift reagent and chemical marker of tissue extracellular space (ECS). The technique was used to determine [Na+]i and relative ECS in livers of control rats (21 +/- 3 and 0.11 +/- 0.02 mM, respectively) and in rats exposed to carbon tetrachloride (103 +/- 29 and 0.23 +/- 0.03 mM, respectively). The NMR measurements were confirmed independently on excised tissue samples by using atomic absorption spectroscopy. The results confirm that TmDOTP5- can be used as a combined cation shift reagent and ECS marker, thereby allowing quantitation of [Na+]i in vivo by NMR.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Texas</li></region></list><tree><noCountry><name sortKey="Bansal, N" sort="Bansal, N" uniqKey="Bansal N" first="N" last="Bansal">N. Bansal</name><name sortKey="Makos, J D" sort="Makos, J D" uniqKey="Makos J" first="J D" last="Makos">J D Makos</name><name sortKey="Malloy, C R" sort="Malloy, C R" uniqKey="Malloy C" first="C R" last="Malloy">C R Malloy</name><name sortKey="Seshan, V" sort="Seshan, V" uniqKey="Seshan V" first="V" last="Seshan">V. Seshan</name><name sortKey="Sherry, A D" sort="Sherry, A D" uniqKey="Sherry A" first="A D" last="Sherry">A D Sherry</name></noCountry><country name="États-Unis"><region name="Texas"><name sortKey="Winter, P M" sort="Winter, P M" uniqKey="Winter P" first="P M" last="Winter">P M Winter</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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