ThuliumV1, Ncbi, Merge, bibRecord, 000C73

Quantitation of intracellular [Na+] in vivo by using TmDOTP5- as an NMR shift reagent and extracellular marker.

Identifieur interne : 000C73 ( Ncbi/Merge ); précédent : 000C72; suivant : 000C74

Quantitation of intracellular [Na+] in vivo by using TmDOTP5- as an NMR shift reagent and extracellular marker.

Auteurs : P M Winter [États-Unis] ; V. Seshan ; J D Makos ; A D Sherry ; C R Malloy ; N. Bansal

Source :

Journal of applied physiology (Bethesda, Md. : 1985) [ 8750-7587 ] ; 1998.

RBID : pubmed:9804585

Descripteurs français

English descriptors

KwdEn :
- Animals, Calibration, Extracellular Space (metabolism), Indicators and Reagents, Magnetic Resonance Spectroscopy, Male, Organometallic Compounds, Organophosphorus Compounds, Phosphorus Radioisotopes, Rats, Rats, Sprague-Dawley, Sodium (metabolism), Sodium Radioisotopes, Spectrophotometry, Atomic, Thulium.
MESH :
- chemical , metabolism : Sodium.
- chemical : Indicators and Reagents, Organometallic Compounds, Organophosphorus Compounds, Phosphorus Radioisotopes, Sodium Radioisotopes, Thulium.
- metabolism : Extracellular Space.
- Animals, Calibration, Magnetic Resonance Spectroscopy, Male, Rats, Rats, Sprague-Dawley, Spectrophotometry, Atomic.

Abstract

A method is presented to measure the absolute concentration of intracellular Na+ ([Na+]i) in vivo by using interleaved 23Na- and 31P-nuclear magnetic resonance (NMR) spectroscopy and TmDOTP5- as shift reagent and chemical marker of tissue extracellular space (ECS). The technique was used to determine [Na+]i and relative ECS in livers of control rats (21 +/- 3 and 0.11 +/- 0.02 mM, respectively) and in rats exposed to carbon tetrachloride (103 +/- 29 and 0.23 +/- 0.03 mM, respectively). The NMR measurements were confirmed independently on excised tissue samples by using atomic absorption spectroscopy. The results confirm that TmDOTP5- can be used as a combined cation shift reagent and ECS marker, thereby allowing quantitation of [Na+]i in vivo by NMR.

PubMed: 9804585

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pubmed:9804585

Le document en format XML

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<author><name sortKey="Winter, P M" sort="Winter, P M" uniqKey="Winter P" first="P M" last="Winter">P M Winter</name>
<affiliation wicri:level="2"><nlm:affiliation>Biomedical Engineering Program, University of Texas at Arlington, Arlington 76019, Texas, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Biomedical Engineering Program, University of Texas at Arlington, Arlington 76019, Texas</wicri:regionArea>
<placeName><region type="state">Texas</region>
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<author><name sortKey="Seshan, V" sort="Seshan, V" uniqKey="Seshan V" first="V" last="Seshan">V. Seshan</name>
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<author><name sortKey="Makos, J D" sort="Makos, J D" uniqKey="Makos J" first="J D" last="Makos">J D Makos</name>
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<author><name sortKey="Sherry, A D" sort="Sherry, A D" uniqKey="Sherry A" first="A D" last="Sherry">A D Sherry</name>
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<author><name sortKey="Malloy, C R" sort="Malloy, C R" uniqKey="Malloy C" first="C R" last="Malloy">C R Malloy</name>
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<author><name sortKey="Bansal, N" sort="Bansal, N" uniqKey="Bansal N" first="N" last="Bansal">N. Bansal</name>
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<term>Magnetic Resonance Spectroscopy</term>
<term>Male</term>
<term>Organometallic Compounds</term>
<term>Organophosphorus Compounds</term>
<term>Phosphorus Radioisotopes</term>
<term>Rats</term>
<term>Rats, Sprague-Dawley</term>
<term>Sodium (metabolism)</term>
<term>Sodium Radioisotopes</term>
<term>Spectrophotometry, Atomic</term>
<term>Thulium</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Calibrage</term>
<term>Composés organiques du phosphore</term>
<term>Composés organométalliques</term>
<term>Espace extracellulaire (métabolisme)</term>
<term>Indicateurs et réactifs</term>
<term>Mâle</term>
<term>Radio-isotopes du phosphore</term>
<term>Radio-isotopes du sodium</term>
<term>Rat Sprague-Dawley</term>
<term>Rats</term>
<term>Sodium (métabolisme)</term>
<term>Spectrophotométrie atomique</term>
<term>Spectroscopie par résonance magnétique</term>
<term>Thulium</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Sodium</term>
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<term>Calibrage</term>
<term>Composés organiques du phosphore</term>
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<term>Indicateurs et réactifs</term>
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<term>Radio-isotopes du phosphore</term>
<term>Radio-isotopes du sodium</term>
<term>Rat Sprague-Dawley</term>
<term>Rats</term>
<term>Spectrophotométrie atomique</term>
<term>Spectroscopie par résonance magnétique</term>
<term>Thulium</term>
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<front><div type="abstract" xml:lang="en">A method is presented to measure the absolute concentration of intracellular Na+ ([Na+]i) in vivo by using interleaved 23Na- and 31P-nuclear magnetic resonance (NMR) spectroscopy and TmDOTP5- as shift reagent and chemical marker of tissue extracellular space (ECS). The technique was used to determine [Na+]i and relative ECS in livers of control rats (21 +/- 3 and 0.11 +/- 0.02 mM, respectively) and in rats exposed to carbon tetrachloride (103 +/- 29 and 0.23 +/- 0.03 mM, respectively). The NMR measurements were confirmed independently on excised tissue samples by using atomic absorption spectroscopy. The results confirm that TmDOTP5- can be used as a combined cation shift reagent and ECS marker, thereby allowing quantitation of [Na+]i in vivo by NMR.</div>
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<Day>29</Day>
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<Abstract><AbstractText>A method is presented to measure the absolute concentration of intracellular Na+ ([Na+]i) in vivo by using interleaved 23Na- and 31P-nuclear magnetic resonance (NMR) spectroscopy and TmDOTP5- as shift reagent and chemical marker of tissue extracellular space (ECS). The technique was used to determine [Na+]i and relative ECS in livers of control rats (21 +/- 3 and 0.11 +/- 0.02 mM, respectively) and in rats exposed to carbon tetrachloride (103 +/- 29 and 0.23 +/- 0.03 mM, respectively). The NMR measurements were confirmed independently on excised tissue samples by using atomic absorption spectroscopy. The results confirm that TmDOTP5- can be used as a combined cation shift reagent and ECS marker, thereby allowing quantitation of [Na+]i in vivo by NMR.</AbstractText>
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<name sortKey="Makos, J D" sort="Makos, J D" uniqKey="Makos J" first="J D" last="Makos">J D Makos</name>
<name sortKey="Malloy, C R" sort="Malloy, C R" uniqKey="Malloy C" first="C R" last="Malloy">C R Malloy</name>
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<name sortKey="Sherry, A D" sort="Sherry, A D" uniqKey="Sherry A" first="A D" last="Sherry">A D Sherry</name>
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<country name="États-Unis"><region name="Texas"><name sortKey="Winter, P M" sort="Winter, P M" uniqKey="Winter P" first="P M" last="Winter">P M Winter</name>
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Quantitation of intracellular [Na+] in vivo by using TmDOTP5- as an NMR shift reagent and extracellular marker.

Quantitation of intracellular [Na+] in vivo by using TmDOTP5- as an NMR shift reagent and extracellular marker.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki