Solid-state NMR studies of magnetically aligned phospholipid membranes: taming lanthanides for membrane protein studies.
Identifieur interne : 002677 ( Main/Exploration ); précédent : 002676; suivant : 002678Solid-state NMR studies of magnetically aligned phospholipid membranes: taming lanthanides for membrane protein studies.
Auteurs : R S Prosser [États-Unis] ; V B Volkov ; I V ShiyanovskayaSource :
- Biochemistry and cell biology = Biochimie et biologie cellulaire [ 0829-8211 ] ; 1998.
Descripteurs français
- KwdFr :
- Acide pentétique (), Chimie physique, Chélateurs (), Dimyristoylphosphatidylcholine (), Double couche lipidique (), Lipides membranaires (), Micelles, Modèles moléculaires, Peptides (), Phosphatidylglycérol (), Phosphatidyléthanolamine (), Phospholipides (), Protéines membranaires (), Spectroscopie par résonance magnétique (), Terres rares (), Thulium (), Ytterbium (), Éther-phospholipides ().
- MESH :
- Acide pentétique, Chimie physique, Chélateurs, Dimyristoylphosphatidylcholine, Double couche lipidique, Lipides membranaires, Micelles, Modèles moléculaires, Peptides, Phosphatidylglycérol, Phosphatidyléthanolamine, Phospholipides, Protéines membranaires, Spectroscopie par résonance magnétique, Terres rares, Thulium, Ytterbium, Éther-phospholipides.
English descriptors
- KwdEn :
- Chelating Agents (chemistry), Chemistry, Physical, Dimyristoylphosphatidylcholine (chemistry), Lipid Bilayers (chemistry), Magnetic Resonance Spectroscopy (methods), Membrane Lipids (chemistry), Membrane Proteins (chemistry), Metals, Rare Earth (chemistry), Micelles, Models, Molecular, Pentetic Acid (chemistry), Peptides (chemistry), Phosphatidylethanolamines (chemistry), Phosphatidylglycerols (chemistry), Phospholipid Ethers (chemistry), Phospholipids (chemistry), Physicochemical Phenomena, Thulium (chemistry), Ytterbium (chemistry).
- MESH :
- chemical , chemistry : Chelating Agents, Dimyristoylphosphatidylcholine, Lipid Bilayers, Membrane Lipids, Membrane Proteins, Metals, Rare Earth, Pentetic Acid, Peptides, Phosphatidylethanolamines, Phosphatidylglycerols, Phospholipid Ethers, Phospholipids, Thulium, Ytterbium.
- methods : Magnetic Resonance Spectroscopy.
- Chemistry, Physical, Micelles, Models, Molecular, Physicochemical Phenomena.
Abstract
The addition of lanthanides (Tm3+, Yb3+, Er3+, or Eu3+) to a solution of long-chain phospholipids such as dimyristoylphosphatidylcholine (DMPC) and short-chain phospholipids such as dihexanoylphosphatidylcholine (DHPC) is known to result in a bilayer phase in which the average bilayer normal aligns parallel to an applied magnetic field. Lanthanide-doped bilayers have enormous potential for the study of membrane proteins by solid-state NMR, low-angle diffraction, and a variety of optical spectroscopic techniques. However, the addition of lanthanides poses certain challenges to the NMR spectroscopist: coexistence of an isotropic phase and hysteresis effects, direct binding of the paramagnetic ion to the peptide or protein of interest, and severe paramagnetic shifts and line broadening. Lower water concentrations and larger DMPC/DHPC ratios than those typically used in bicelles consistently yield a single oriented bilayer phase that is stable over a wide range of temperature (approximately 35-90 degrees C). Among the above choice of lanthanides, Yb3+ is found to give minimal paramagnetic shifts and line broadening at acceptably low concentrations necessary for alignment (i.e., Yb3+/DMPC mole ratios equal to or greater than 0.01). Finally, the addition of a phospholipid chelate, 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine--diethylenetriaminepent aacetic acid, is observed to significantly reduce paramagnetic broadening and presumably prevent direct association of the peptide with the lanthanide ions.
PubMed: 9923713
Affiliations:
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type="wicri:Area/Main/Exploration">002677</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Solid-state NMR studies of magnetically aligned phospholipid membranes: taming lanthanides for membrane protein studies.</title><author><name sortKey="Prosser, R S" sort="Prosser, R S" uniqKey="Prosser R" first="R S" last="Prosser">R S Prosser</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemistry, Kent State University, OH 44242, USA. sprosser@silica.kent.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, Kent State University, OH 44242</wicri:regionArea><placeName><region type="state">Ohio</region></placeName></affiliation></author><author><name sortKey="Volkov, V B" sort="Volkov, V B" uniqKey="Volkov V" first="V B" last="Volkov">V B Volkov</name></author><author><name sortKey="Shiyanovskaya, I V" sort="Shiyanovskaya, I V" uniqKey="Shiyanovskaya I" first="I V" last="Shiyanovskaya">I V Shiyanovskaya</name></author></analytic><series><title level="j">Biochemistry and cell biology = Biochimie et biologie cellulaire</title><idno type="ISSN">0829-8211</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chelating Agents (chemistry)</term><term>Chemistry, Physical</term><term>Dimyristoylphosphatidylcholine (chemistry)</term><term>Lipid Bilayers (chemistry)</term><term>Magnetic Resonance Spectroscopy (methods)</term><term>Membrane Lipids (chemistry)</term><term>Membrane Proteins (chemistry)</term><term>Metals, Rare Earth (chemistry)</term><term>Micelles</term><term>Models, Molecular</term><term>Pentetic Acid (chemistry)</term><term>Peptides (chemistry)</term><term>Phosphatidylethanolamines (chemistry)</term><term>Phosphatidylglycerols (chemistry)</term><term>Phospholipid Ethers (chemistry)</term><term>Phospholipids (chemistry)</term><term>Physicochemical Phenomena</term><term>Thulium (chemistry)</term><term>Ytterbium (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide pentétique ()</term><term>Chimie physique</term><term>Chélateurs ()</term><term>Dimyristoylphosphatidylcholine ()</term><term>Double couche lipidique ()</term><term>Lipides membranaires ()</term><term>Micelles</term><term>Modèles moléculaires</term><term>Peptides ()</term><term>Phosphatidylglycérol ()</term><term>Phosphatidyléthanolamine ()</term><term>Phospholipides ()</term><term>Protéines membranaires ()</term><term>Spectroscopie par résonance magnétique ()</term><term>Terres rares ()</term><term>Thulium ()</term><term>Ytterbium ()</term><term>Éther-phospholipides ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Chelating Agents</term><term>Dimyristoylphosphatidylcholine</term><term>Lipid Bilayers</term><term>Membrane Lipids</term><term>Membrane Proteins</term><term>Metals, Rare Earth</term><term>Pentetic Acid</term><term>Peptides</term><term>Phosphatidylethanolamines</term><term>Phosphatidylglycerols</term><term>Phospholipid Ethers</term><term>Phospholipids</term><term>Thulium</term><term>Ytterbium</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Magnetic Resonance Spectroscopy</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chemistry, Physical</term><term>Micelles</term><term>Models, Molecular</term><term>Physicochemical Phenomena</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Acide pentétique</term><term>Chimie physique</term><term>Chélateurs</term><term>Dimyristoylphosphatidylcholine</term><term>Double couche lipidique</term><term>Lipides membranaires</term><term>Micelles</term><term>Modèles moléculaires</term><term>Peptides</term><term>Phosphatidylglycérol</term><term>Phosphatidyléthanolamine</term><term>Phospholipides</term><term>Protéines membranaires</term><term>Spectroscopie par résonance magnétique</term><term>Terres rares</term><term>Thulium</term><term>Ytterbium</term><term>Éther-phospholipides</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The addition of lanthanides (Tm3+, Yb3+, Er3+, or Eu3+) to a solution of long-chain phospholipids such as dimyristoylphosphatidylcholine (DMPC) and short-chain phospholipids such as dihexanoylphosphatidylcholine (DHPC) is known to result in a bilayer phase in which the average bilayer normal aligns parallel to an applied magnetic field. Lanthanide-doped bilayers have enormous potential for the study of membrane proteins by solid-state NMR, low-angle diffraction, and a variety of optical spectroscopic techniques. However, the addition of lanthanides poses certain challenges to the NMR spectroscopist: coexistence of an isotropic phase and hysteresis effects, direct binding of the paramagnetic ion to the peptide or protein of interest, and severe paramagnetic shifts and line broadening. Lower water concentrations and larger DMPC/DHPC ratios than those typically used in bicelles consistently yield a single oriented bilayer phase that is stable over a wide range of temperature (approximately 35-90 degrees C). Among the above choice of lanthanides, Yb3+ is found to give minimal paramagnetic shifts and line broadening at acceptably low concentrations necessary for alignment (i.e., Yb3+/DMPC mole ratios equal to or greater than 0.01). Finally, the addition of a phospholipid chelate, 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine--diethylenetriaminepent aacetic acid, is observed to significantly reduce paramagnetic broadening and presumably prevent direct association of the peptide with the lanthanide ions.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Ohio</li></region></list><tree><noCountry><name sortKey="Shiyanovskaya, I V" sort="Shiyanovskaya, I V" uniqKey="Shiyanovskaya I" first="I V" last="Shiyanovskaya">I V Shiyanovskaya</name><name sortKey="Volkov, V B" sort="Volkov, V B" uniqKey="Volkov V" first="V B" last="Volkov">V B Volkov</name></noCountry><country name="États-Unis"><region name="Ohio"><name sortKey="Prosser, R S" sort="Prosser, R S" uniqKey="Prosser R" first="R S" last="Prosser">R S Prosser</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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