Calcium imaging of infrared-stimulated activity in rodent brain
Identifieur interne : 000347 ( Main/Exploration ); précédent : 000346; suivant : 000348Calcium imaging of infrared-stimulated activity in rodent brain
Auteurs : Jonathan Matthew Cayce [États-Unis] ; Matthew B. Bouchard [États-Unis] ; Mykyta M. Chernov [États-Unis] ; Brenda R. Chen [États-Unis] ; Lauren E. Grosberg [États-Unis] ; E. Duco Jansen [États-Unis] ; Elizabeth M. C. Hillman [États-Unis] ; Anita Mahadevan-Jansen [États-Unis]Source :
- Cell calcium [ 0143-4160 ] ; 2014.
Abstract
Infrared neural stimulation (INS) is a promising neurostimulation technique that can activate neural tissue with high spatial precision and without the need for exogenous agents. However, little is understood about how infrared light interacts with neural tissue on a cellular level, particularly within the living brain. In this study, we use calcium sensitive dye imaging on macroscopic and microscopic scales to explore the spatiotemporal effects of INS on cortical calcium dynamics. The INS-evoked calcium signal that was observed exhibited a fast and slow component suggesting activation of multiple cellular mechanisms. The slow component of the evoked signal exhibited wave-like properties suggesting network activation, and was verified to originate from astrocytes through pharmacology and 2-photon imaging. We also provide evidence that the fast calcium signal may have been evoked through modulation of glutamate transients. This study demonstrates that pulsed infrared light can induce intracellular calcium modulations in both astrocytes and neurons, providing new insights into the mechanisms of action of INS in the brain.
Url:
DOI: 10.1016/j.ceca.2014.01.004
PubMed: 24674600
PubMed Central: 4014070
Affiliations:
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Chernov</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Biomedical Engineering, Vanderbilt University, Station B, Box 351631, Nashville, TN 37235</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Biomedical Engineering, Vanderbilt University, Station B, Box 351631, Nashville</wicri:cityArea></affiliation></author><author><name sortKey="Chen, Brenda R" sort="Chen, Brenda R" uniqKey="Chen B" first="Brenda R." last="Chen">Brenda R. 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Grosberg</name><affiliation wicri:level="2"><nlm:aff id="A2">Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Columbia University, 120th Street and Amsterdam Ave New York, NY 10027</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Columbia University, 120th Street and Amsterdam Ave New York</wicri:cityArea></affiliation></author><author><name sortKey="Jansen, E Duco" sort="Jansen, E Duco" uniqKey="Jansen E" first="E. Duco" last="Jansen">E. Duco Jansen</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Biomedical Engineering, Vanderbilt University, Station B, Box 351631, Nashville, TN 37235</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Biomedical Engineering, Vanderbilt University, Station B, Box 351631, Nashville</wicri:cityArea></affiliation></author><author><name sortKey="Hillman, Elizabeth M C" sort="Hillman, Elizabeth M C" uniqKey="Hillman E" first="Elizabeth M. C." last="Hillman">Elizabeth M. C. Hillman</name><affiliation wicri:level="2"><nlm:aff id="A2">Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Columbia University, 120th Street and Amsterdam Ave New York, NY 10027</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Laboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology, Columbia University, 120th Street and Amsterdam Ave New York</wicri:cityArea></affiliation></author><author><name sortKey="Mahadevan Jansen, Anita" sort="Mahadevan Jansen, Anita" uniqKey="Mahadevan Jansen A" first="Anita" last="Mahadevan-Jansen">Anita Mahadevan-Jansen</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Biomedical Engineering, Vanderbilt University, Station B, Box 351631, Nashville, TN 37235</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Biomedical Engineering, Vanderbilt University, Station B, Box 351631, Nashville</wicri:cityArea></affiliation></author></analytic><series><title level="j">Cell calcium</title><idno type="ISSN">0143-4160</idno><idno type="eISSN">1532-1991</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Summary</title><p id="P3">Infrared neural stimulation (INS) is a promising neurostimulation technique that can activate neural tissue with high spatial precision and without the need for exogenous agents. However, little is understood about how infrared light interacts with neural tissue on a cellular level, particularly within the living brain. In this study, we use calcium sensitive dye imaging on macroscopic and microscopic scales to explore the spatiotemporal effects of INS on cortical calcium dynamics. The INS-evoked calcium signal that was observed exhibited a fast and slow component suggesting activation of multiple cellular mechanisms. The slow component of the evoked signal exhibited wave-like properties suggesting network activation, and was verified to originate from astrocytes through pharmacology and 2-photon imaging. We also provide evidence that the fast calcium signal may have been evoked through modulation of glutamate transients. This study demonstrates that pulsed infrared light can induce intracellular calcium modulations in both astrocytes and neurons, providing new insights into the mechanisms of action of INS in the brain.</p></div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Tennessee</li><li>État de New York</li></region></list><tree><country name="États-Unis"><region name="Tennessee"><name sortKey="Cayce, Jonathan Matthew" sort="Cayce, Jonathan Matthew" uniqKey="Cayce J" first="Jonathan Matthew" last="Cayce">Jonathan Matthew Cayce</name></region><name sortKey="Bouchard, Matthew B" sort="Bouchard, Matthew B" uniqKey="Bouchard M" first="Matthew B." last="Bouchard">Matthew B. Bouchard</name><name sortKey="Chen, Brenda R" sort="Chen, Brenda R" uniqKey="Chen B" first="Brenda R." last="Chen">Brenda R. Chen</name><name sortKey="Chernov, Mykyta M" sort="Chernov, Mykyta M" uniqKey="Chernov M" first="Mykyta M." last="Chernov">Mykyta M. Chernov</name><name sortKey="Grosberg, Lauren E" sort="Grosberg, Lauren E" uniqKey="Grosberg L" first="Lauren E." last="Grosberg">Lauren E. Grosberg</name><name sortKey="Hillman, Elizabeth M C" sort="Hillman, Elizabeth M C" uniqKey="Hillman E" first="Elizabeth M. C." last="Hillman">Elizabeth M. C. Hillman</name><name sortKey="Jansen, E Duco" sort="Jansen, E Duco" uniqKey="Jansen E" first="E. Duco" last="Jansen">E. Duco Jansen</name><name sortKey="Mahadevan Jansen, Anita" sort="Mahadevan Jansen, Anita" uniqKey="Mahadevan Jansen A" first="Anita" last="Mahadevan-Jansen">Anita Mahadevan-Jansen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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