An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates.
Identifieur interne : 000C64 ( Main/Corpus ); précédent : 000C63; suivant : 000C65An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates.
Auteurs : D A Spratt ; J. Pratten ; M. Wilson ; K. GulabivalaSource :
- International endodontic journal [ 0143-2885 ] ; 2001.
English descriptors
- KwdEn :
- Anti-Infective Agents, Local (pharmacology), Bacteriological Techniques (MeSH), Biofilms (drug effects), Biofilms (growth & development), Buffers (MeSH), Chlorhexidine (pharmacology), Colony Count, Microbial (MeSH), Dental Pulp Cavity (microbiology), Enterococcus faecalis (drug effects), Fusobacterium nucleatum (drug effects), Humans (MeSH), Iodine (pharmacology), Peptostreptococcus (drug effects), Prevotella intermedia (drug effects), Root Canal Irrigants (pharmacology), Silver (pharmacology), Sodium Chloride (MeSH), Sodium Hypochlorite (pharmacology), Streptococcus (drug effects), Time Factors (MeSH).
- MESH :
- chemical , pharmacology : Anti-Infective Agents, Local, Chlorhexidine, Iodine, Root Canal Irrigants, Silver, Sodium Hypochlorite.
- drug effects : Biofilms, Enterococcus faecalis, Fusobacterium nucleatum, Peptostreptococcus, Prevotella intermedia, Streptococcus.
- growth & development : Biofilms.
- microbiology : Dental Pulp Cavity.
- Bacteriological Techniques, Buffers, Colony Count, Microbial, Humans, Sodium Chloride, Time Factors.
Abstract
AIM
The bactericidal effect of four antimicrobial agents was investigated against single-species biofilms derived from a range of root canal isolates.
METHODOLOGY
Single-species biofilms of Prevotella intermedia, Peptostreptococcus micros, Streptococcus intermedius, Fusobacterium nucleatum and Enterococcus faecalis were generated on membrane filter discs and subjected to 15 min or 1 h incubation with 5 p.p.m. colloidal silver, 2.25% sodium hypochlorite (NaOCl), 0.2% chlorhexidine, 10% iodine or phosphate buffered saline (PBS) as a control. The antimicrobial activity of the agents was neutralized and the bacterial cells were harvested from the discs by vortexing, serially diluted in reduced transport fluid, plated on fastidious anaerobe agar containing 5% horse blood, incubated anaerobically and colony-forming units calculated.
RESULTS
Iodine and NaOCl were more effective than chlorhexidine except against P. micros and P. intermedia where they were all 100% effective. Iodine and NaOCl elicited a 100% kill after 1 h incubation for all strains used. However, after 15 min, they showed differing bactericidal effects depending on the strain. None of the agents were effective against F. nucleatum after 15 min but NaOCl, iodine and chlorhexidine were all effective after 1 h. Colloidal silver was generally ineffective.
CONCLUSIONS
The effectiveness of a particular agent was dependent on the nature of the organism in the biofilm and on the contact time. NaOCl was generally the most effective agent tested, followed by iodine. However the clinical efficacy of these agents must be considered in light of the complex root canal anatomy and polymicrobial nature of root canal infections.
DOI: 10.1046/j.1365-2591.2001.00392.x
PubMed: 11482142
Links to Exploration step
pubmed:11482142Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates.</title>
<author><name sortKey="Spratt, D A" sort="Spratt, D A" uniqKey="Spratt D" first="D A" last="Spratt">D A Spratt</name>
<affiliation><nlm:affiliation>Department of Conservative Dentistry, Eastman Dental Institute for Oral Health Care Sciences, University College London, University of London, London, UK. d.spratt@eastman.ucl.ac.uk</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Pratten, J" sort="Pratten, J" uniqKey="Pratten J" first="J" last="Pratten">J. Pratten</name>
</author>
<author><name sortKey="Wilson, M" sort="Wilson, M" uniqKey="Wilson M" first="M" last="Wilson">M. Wilson</name>
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<author><name sortKey="Gulabivala, K" sort="Gulabivala, K" uniqKey="Gulabivala K" first="K" last="Gulabivala">K. Gulabivala</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates.</title>
<author><name sortKey="Spratt, D A" sort="Spratt, D A" uniqKey="Spratt D" first="D A" last="Spratt">D A Spratt</name>
<affiliation><nlm:affiliation>Department of Conservative Dentistry, Eastman Dental Institute for Oral Health Care Sciences, University College London, University of London, London, UK. d.spratt@eastman.ucl.ac.uk</nlm:affiliation>
</affiliation>
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<author><name sortKey="Pratten, J" sort="Pratten, J" uniqKey="Pratten J" first="J" last="Pratten">J. Pratten</name>
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<author><name sortKey="Wilson, M" sort="Wilson, M" uniqKey="Wilson M" first="M" last="Wilson">M. Wilson</name>
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<author><name sortKey="Gulabivala, K" sort="Gulabivala, K" uniqKey="Gulabivala K" first="K" last="Gulabivala">K. Gulabivala</name>
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<series><title level="j">International endodontic journal</title>
<idno type="ISSN">0143-2885</idno>
<imprint><date when="2001" type="published">2001</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-Infective Agents, Local (pharmacology)</term>
<term>Bacteriological Techniques (MeSH)</term>
<term>Biofilms (drug effects)</term>
<term>Biofilms (growth & development)</term>
<term>Buffers (MeSH)</term>
<term>Chlorhexidine (pharmacology)</term>
<term>Colony Count, Microbial (MeSH)</term>
<term>Dental Pulp Cavity (microbiology)</term>
<term>Enterococcus faecalis (drug effects)</term>
<term>Fusobacterium nucleatum (drug effects)</term>
<term>Humans (MeSH)</term>
<term>Iodine (pharmacology)</term>
<term>Peptostreptococcus (drug effects)</term>
<term>Prevotella intermedia (drug effects)</term>
<term>Root Canal Irrigants (pharmacology)</term>
<term>Silver (pharmacology)</term>
<term>Sodium Chloride (MeSH)</term>
<term>Sodium Hypochlorite (pharmacology)</term>
<term>Streptococcus (drug effects)</term>
<term>Time Factors (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Infective Agents, Local</term>
<term>Chlorhexidine</term>
<term>Iodine</term>
<term>Root Canal Irrigants</term>
<term>Silver</term>
<term>Sodium Hypochlorite</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Biofilms</term>
<term>Enterococcus faecalis</term>
<term>Fusobacterium nucleatum</term>
<term>Peptostreptococcus</term>
<term>Prevotella intermedia</term>
<term>Streptococcus</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Biofilms</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Dental Pulp Cavity</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Bacteriological Techniques</term>
<term>Buffers</term>
<term>Colony Count, Microbial</term>
<term>Humans</term>
<term>Sodium Chloride</term>
<term>Time Factors</term>
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<front><div type="abstract" xml:lang="en"><p><b>AIM</b>
</p>
<p>The bactericidal effect of four antimicrobial agents was investigated against single-species biofilms derived from a range of root canal isolates.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>METHODOLOGY</b>
</p>
<p>Single-species biofilms of Prevotella intermedia, Peptostreptococcus micros, Streptococcus intermedius, Fusobacterium nucleatum and Enterococcus faecalis were generated on membrane filter discs and subjected to 15 min or 1 h incubation with 5 p.p.m. colloidal silver, 2.25% sodium hypochlorite (NaOCl), 0.2% chlorhexidine, 10% iodine or phosphate buffered saline (PBS) as a control. The antimicrobial activity of the agents was neutralized and the bacterial cells were harvested from the discs by vortexing, serially diluted in reduced transport fluid, plated on fastidious anaerobe agar containing 5% horse blood, incubated anaerobically and colony-forming units calculated.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>Iodine and NaOCl were more effective than chlorhexidine except against P. micros and P. intermedia where they were all 100% effective. Iodine and NaOCl elicited a 100% kill after 1 h incubation for all strains used. However, after 15 min, they showed differing bactericidal effects depending on the strain. None of the agents were effective against F. nucleatum after 15 min but NaOCl, iodine and chlorhexidine were all effective after 1 h. Colloidal silver was generally ineffective.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b>
</p>
<p>The effectiveness of a particular agent was dependent on the nature of the organism in the biofilm and on the contact time. NaOCl was generally the most effective agent tested, followed by iodine. However the clinical efficacy of these agents must be considered in light of the complex root canal anatomy and polymicrobial nature of root canal infections.</p>
</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11482142</PMID>
<DateCompleted><Year>2001</Year>
<Month>09</Month>
<Day>20</Day>
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<DateRevised><Year>2019</Year>
<Month>09</Month>
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<Issue>4</Issue>
<PubDate><Year>2001</Year>
<Month>Jun</Month>
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<Title>International endodontic journal</Title>
<ISOAbbreviation>Int Endod J</ISOAbbreviation>
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<ArticleTitle>An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates.</ArticleTitle>
<Pagination><MedlinePgn>300-7</MedlinePgn>
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<Abstract><AbstractText Label="AIM" NlmCategory="OBJECTIVE">The bactericidal effect of four antimicrobial agents was investigated against single-species biofilms derived from a range of root canal isolates.</AbstractText>
<AbstractText Label="METHODOLOGY" NlmCategory="METHODS">Single-species biofilms of Prevotella intermedia, Peptostreptococcus micros, Streptococcus intermedius, Fusobacterium nucleatum and Enterococcus faecalis were generated on membrane filter discs and subjected to 15 min or 1 h incubation with 5 p.p.m. colloidal silver, 2.25% sodium hypochlorite (NaOCl), 0.2% chlorhexidine, 10% iodine or phosphate buffered saline (PBS) as a control. The antimicrobial activity of the agents was neutralized and the bacterial cells were harvested from the discs by vortexing, serially diluted in reduced transport fluid, plated on fastidious anaerobe agar containing 5% horse blood, incubated anaerobically and colony-forming units calculated.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Iodine and NaOCl were more effective than chlorhexidine except against P. micros and P. intermedia where they were all 100% effective. Iodine and NaOCl elicited a 100% kill after 1 h incubation for all strains used. However, after 15 min, they showed differing bactericidal effects depending on the strain. None of the agents were effective against F. nucleatum after 15 min but NaOCl, iodine and chlorhexidine were all effective after 1 h. Colloidal silver was generally ineffective.</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The effectiveness of a particular agent was dependent on the nature of the organism in the biofilm and on the contact time. NaOCl was generally the most effective agent tested, followed by iodine. However the clinical efficacy of these agents must be considered in light of the complex root canal anatomy and polymicrobial nature of root canal infections.</AbstractText>
</Abstract>
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<Language>eng</Language>
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<MedlineJournalInfo><Country>England</Country>
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