Silver nanoparticles as an antimicrobial agent: A case study on Staphylococcus aureus and Escherichia coli as models for Gram-positive and Gram-negative bacteria.
Identifieur interne : 000587 ( Main/Corpus ); précédent : 000586; suivant : 000588Silver nanoparticles as an antimicrobial agent: A case study on Staphylococcus aureus and Escherichia coli as models for Gram-positive and Gram-negative bacteria.
Auteurs : Eman Zakaria GomaaSource :
- The Journal of general and applied microbiology [ 1349-8037 ] ; 2017.
English descriptors
- KwdEn :
- Anti-Bacterial Agents (analysis), Anti-Bacterial Agents (pharmacology), Bacillus licheniformis (metabolism), Cell Membrane (drug effects), Cell Membrane (enzymology), Cell Membrane (physiology), Escherichia coli (drug effects), Escherichia coli (physiology), Microbial Viability (drug effects), Microscopy, Electron, Transmission (MeSH), Nanoparticles (chemistry), Nanoparticles (metabolism), Nanoparticles (ultrastructure), Permeability (MeSH), Silver (metabolism), Silver (pharmacology), Spectrometry, X-Ray Emission (MeSH), Spectrophotometry (MeSH), Spectroscopy, Fourier Transform Infrared (MeSH), Staphylococcus aureus (drug effects), Staphylococcus aureus (physiology).
- MESH :
- chemical , analysis : Anti-Bacterial Agents.
- chemical , metabolism : Silver.
- chemical , pharmacology : Anti-Bacterial Agents, Silver.
- chemistry : Nanoparticles.
- drug effects : Cell Membrane, Escherichia coli, Microbial Viability, Staphylococcus aureus.
- enzymology : Cell Membrane.
- metabolism : Bacillus licheniformis, Nanoparticles.
- physiology : Cell Membrane, Escherichia coli, Staphylococcus aureus.
- ultrastructure : Nanoparticles.
- Microscopy, Electron, Transmission, Permeability, Spectrometry, X-Ray Emission, Spectrophotometry, Spectroscopy, Fourier Transform Infrared.
Abstract
The current research was focused on the characterization and antimicrobial activity of silver nanoparticles (AgNPs) produced by Bacillus licheniformis NM120-17. The synthesis was initially observed by a colour change from pale yellow to brown which was further confirmed by UV-Vis spectroscopy. The AgNPs were characterized using TEM, EDAX and FTIR. The synthesized nanoparticles were found to be spherical and uniformly distributed with a size in the range of 9-27.5 nm. The antibacterial activities and acting mechanism of AgNPs were studied with respect to Staphylococcus aureus and Escherichia coli by measuring the growth curves, protein and reducing sugar leakage, respiratory chain dehydrogenase activity, as well as the formation of bactericidal reactive oxygen species (ROS). The experimental results indicated that 50 mg/ml AgNPs could completely inhibit the growth of bacterial cells and destroy the permeability of bacterial membranes and depress the activity of some membranous enzymes, which cause bacteria to die eventually. These nontoxic nanomaterials, which can be prepared in a simple and cost-effective manner, may be suitable for the formulation of new types of bactericidal materials.
DOI: 10.2323/jgam.2016.07.004
PubMed: 28123131
Links to Exploration step
pubmed:28123131Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Silver nanoparticles as an antimicrobial agent: A case study on Staphylococcus aureus and Escherichia coli as models for Gram-positive and Gram-negative bacteria.</title><author><name sortKey="Gomaa, Eman Zakaria" sort="Gomaa, Eman Zakaria" uniqKey="Gomaa E" first="Eman Zakaria" last="Gomaa">Eman Zakaria Gomaa</name><affiliation><nlm:affiliation>Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28123131</idno><idno type="pmid">28123131</idno><idno type="doi">10.2323/jgam.2016.07.004</idno><idno type="wicri:Area/Main/Corpus">000587</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000587</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Silver nanoparticles as an antimicrobial agent: A case study on Staphylococcus aureus and Escherichia coli as models for Gram-positive and Gram-negative bacteria.</title><author><name sortKey="Gomaa, Eman Zakaria" sort="Gomaa, Eman Zakaria" uniqKey="Gomaa E" first="Eman Zakaria" last="Gomaa">Eman Zakaria Gomaa</name><affiliation><nlm:affiliation>Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University.</nlm:affiliation></affiliation></author></analytic><series><title level="j">The Journal of general and applied microbiology</title><idno type="eISSN">1349-8037</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-Bacterial Agents (analysis)</term><term>Anti-Bacterial Agents (pharmacology)</term><term>Bacillus licheniformis (metabolism)</term><term>Cell Membrane (drug effects)</term><term>Cell Membrane (enzymology)</term><term>Cell Membrane (physiology)</term><term>Escherichia coli (drug effects)</term><term>Escherichia coli (physiology)</term><term>Microbial Viability (drug effects)</term><term>Microscopy, Electron, Transmission (MeSH)</term><term>Nanoparticles (chemistry)</term><term>Nanoparticles (metabolism)</term><term>Nanoparticles (ultrastructure)</term><term>Permeability (MeSH)</term><term>Silver (metabolism)</term><term>Silver (pharmacology)</term><term>Spectrometry, X-Ray Emission (MeSH)</term><term>Spectrophotometry (MeSH)</term><term>Spectroscopy, Fourier Transform Infrared (MeSH)</term><term>Staphylococcus aureus (drug effects)</term><term>Staphylococcus aureus (physiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Anti-Bacterial Agents</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Silver</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Silver</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Membrane</term><term>Escherichia coli</term><term>Microbial Viability</term><term>Staphylococcus aureus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Cell Membrane</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacillus licheniformis</term><term>Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Membrane</term><term>Escherichia coli</term><term>Staphylococcus aureus</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Nanoparticles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Microscopy, Electron, Transmission</term><term>Permeability</term><term>Spectrometry, X-Ray Emission</term><term>Spectrophotometry</term><term>Spectroscopy, Fourier Transform Infrared</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The current research was focused on the characterization and antimicrobial activity of silver nanoparticles (AgNPs) produced by Bacillus licheniformis NM120-17. The synthesis was initially observed by a colour change from pale yellow to brown which was further confirmed by UV-Vis spectroscopy. The AgNPs were characterized using TEM, EDAX and FTIR. The synthesized nanoparticles were found to be spherical and uniformly distributed with a size in the range of 9-27.5 nm. The antibacterial activities and acting mechanism of AgNPs were studied with respect to Staphylococcus aureus and Escherichia coli by measuring the growth curves, protein and reducing sugar leakage, respiratory chain dehydrogenase activity, as well as the formation of bactericidal reactive oxygen species (ROS). The experimental results indicated that 50 mg/ml AgNPs could completely inhibit the growth of bacterial cells and destroy the permeability of bacterial membranes and depress the activity of some membranous enzymes, which cause bacteria to die eventually. These nontoxic nanomaterials, which can be prepared in a simple and cost-effective manner, may be suitable for the formulation of new types of bactericidal materials.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28123131</PMID><DateCompleted><Year>2017</Year><Month>04</Month><Day>17</Day></DateCompleted><DateRevised><Year>2017</Year><Month>04</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1349-8037</ISSN><JournalIssue CitedMedium="Internet"><Volume>63</Volume><Issue>1</Issue><PubDate><Year>2017</Year><Month>Mar</Month><Day>17</Day></PubDate></JournalIssue><Title>The Journal of general and applied microbiology</Title><ISOAbbreviation>J Gen Appl Microbiol</ISOAbbreviation></Journal><ArticleTitle>Silver nanoparticles as an antimicrobial agent: A case study on Staphylococcus aureus and Escherichia coli as models for Gram-positive and Gram-negative bacteria.</ArticleTitle><Pagination><MedlinePgn>36-43</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.2323/jgam.2016.07.004</ELocationID><Abstract><AbstractText>The current research was focused on the characterization and antimicrobial activity of silver nanoparticles (AgNPs) produced by Bacillus licheniformis NM120-17. The synthesis was initially observed by a colour change from pale yellow to brown which was further confirmed by UV-Vis spectroscopy. The AgNPs were characterized using TEM, EDAX and FTIR. The synthesized nanoparticles were found to be spherical and uniformly distributed with a size in the range of 9-27.5 nm. The antibacterial activities and acting mechanism of AgNPs were studied with respect to Staphylococcus aureus and Escherichia coli by measuring the growth curves, protein and reducing sugar leakage, respiratory chain dehydrogenase activity, as well as the formation of bactericidal reactive oxygen species (ROS). The experimental results indicated that 50 mg/ml AgNPs could completely inhibit the growth of bacterial cells and destroy the permeability of bacterial membranes and depress the activity of some membranous enzymes, which cause bacteria to die eventually. These nontoxic nanomaterials, which can be prepared in a simple and cost-effective manner, may be suitable for the formulation of new types of bactericidal materials.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gomaa</LastName><ForeName>Eman Zakaria</ForeName><Initials>EZ</Initials><AffiliationInfo><Affiliation>Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>01</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>J Gen Appl Microbiol</MedlineTA><NlmUniqueID>0165543</NlmUniqueID><ISSNLinking>0022-1260</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>3M4G523W1G</RegistryNumber><NameOfSubstance UI="D012834">Silver</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000069976" MajorTopicYN="N">Bacillus licheniformis</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002462" MajorTopicYN="N">Cell Membrane</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D050296" MajorTopicYN="N">Microbial Viability</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046529" MajorTopicYN="N">Microscopy, Electron, Transmission</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053758" MajorTopicYN="N">Nanoparticles</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010539" MajorTopicYN="N">Permeability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012834" MajorTopicYN="N">Silver</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013052" MajorTopicYN="N">Spectrometry, X-Ray Emission</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013053" MajorTopicYN="N">Spectrophotometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017550" MajorTopicYN="N">Spectroscopy, Fourier Transform Infrared</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013211" MajorTopicYN="N">Staphylococcus aureus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>1</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>4</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>1</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28123131</ArticleId><ArticleId IdType="doi">10.2323/jgam.2016.07.004</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Terre/explor/SilverBacteriV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000587 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000587 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Terre
|area= SilverBacteriV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:28123131
|texte= Silver nanoparticles as an antimicrobial agent: A case study on Staphylococcus aureus and Escherichia coli as models for Gram-positive and Gram-negative bacteria.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:28123131" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a SilverBacteriV1
| This area was generated with Dilib version V0.6.38. |  |