Stable Nanocomposite Based on PEGylated and Silver Nanoparticles Loaded Graphene Oxide for Long-Term Antibacterial Activity.
Identifieur interne : 000561 ( Main/Corpus ); précédent : 000560; suivant : 000562Stable Nanocomposite Based on PEGylated and Silver Nanoparticles Loaded Graphene Oxide for Long-Term Antibacterial Activity.
Auteurs : Rongtao Zhao ; Min Lv ; Yang Li ; Mingxuan Sun ; Wen Kong ; Lihua Wang ; Shiping Song ; Chunhai Fan ; Leili Jia ; Shaofu Qiu ; Yansong Sun ; Hongbin Song ; Rongzhang HaoSource :
- ACS applied materials & interfaces [ 1944-8252 ] ; 2017.
English descriptors
- KwdEn :
- MESH :
Abstract
The increasing occurrence of antibiotic-resistant pathogens, especially superbugs, is compromising the efficacy of traditional antibiotics. Silver nanoparticles (AgNPs) loaded graphene oxide (GO) nanocomposite (GO-Ag) has drawn great interest as a promising alternative antibacterial material. However, GO-Ag nanocomposite often irreversibly aggregates in physiological solutions, severely influencing its antibacterial capacity and practical application. Herein, a PEGylated and AgNPs loaded GO nanocomposite (GO-PEG-Ag) is synthesized through a facile approach utilizing microwave irradiation, while avoiding extra reducing agents. Through PEGylation, the synthesized GO-PEG-Ag nanocomposite dispersed stably over one month in a series of media and resisted centrifugation at 10 000×g for 5 min, which would benefit effective contact between the nanocomposite and the bacteria. In contrast, GO-Ag aggregated within 1 h of dispersion in physiological solutions. In comparison with GO-Ag, GO-PEG-Ag showed stronger bactericidal capability toward not only normal Gram-negative/positive bacteria such as E. coli and S. aureus (∼100% of E. coli and ∼95.3% of S. aureus reduction by 10 μg/mL nanocomposite for 2.5 h), but also superbugs. Moreover, GO-PEG-Ag showed lower cytotoxicity toward HeLa cells. Importantly, GO-PEG-Ag presented long-term antibacterial effectiveness, remaining ∼95% antibacterial activity after one-week storage in saline solution versus <35% for GO-Ag. The antibacterial mechanisms of GO-PEG-Ag were evidenced as damage to the bacterial structure and production of reactive oxygen species, causing cytoplasm leakage and metabolism decrease. The stable GO-PEG-Ag nanocomposite with powerful and long-term antibacterial capability provides a more practical and effective strategy for fighting superbugs-including pathogen threats in biomedicine and public health.
DOI: 10.1021/acsami.7b03987
PubMed: 28422486
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China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Yang" sort="Li, Yang" uniqKey="Li Y" first="Yang" last="Li">Yang Li</name><affiliation><nlm:affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Sun, Mingxuan" sort="Sun, Mingxuan" uniqKey="Sun M" first="Mingxuan" last="Sun">Mingxuan Sun</name><affiliation><nlm:affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Kong, Wen" sort="Kong, Wen" uniqKey="Kong W" first="Wen" last="Kong">Wen Kong</name><affiliation><nlm:affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Lihua" sort="Wang, Lihua" uniqKey="Wang L" first="Lihua" last="Wang">Lihua Wang</name><affiliation><nlm:affiliation>Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Song, Shiping" sort="Song, Shiping" uniqKey="Song S" first="Shiping" last="Song">Shiping Song</name><affiliation><nlm:affiliation>Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Fan, Chunhai" sort="Fan, Chunhai" uniqKey="Fan C" first="Chunhai" last="Fan">Chunhai Fan</name><affiliation><nlm:affiliation>Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Jia, Leili" sort="Jia, Leili" uniqKey="Jia L" first="Leili" last="Jia">Leili Jia</name><affiliation><nlm:affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Qiu, Shaofu" sort="Qiu, Shaofu" uniqKey="Qiu S" first="Shaofu" last="Qiu">Shaofu Qiu</name><affiliation><nlm:affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Sun, Yansong" sort="Sun, Yansong" uniqKey="Sun Y" first="Yansong" last="Sun">Yansong Sun</name><affiliation><nlm:affiliation>Department of Science and Technology, AMMS , Beijing 100850, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Song, Hongbin" sort="Song, Hongbin" uniqKey="Song H" first="Hongbin" last="Song">Hongbin Song</name><affiliation><nlm:affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Hao, Rongzhang" sort="Hao, Rongzhang" uniqKey="Hao R" first="Rongzhang" last="Hao">Rongzhang Hao</name><affiliation><nlm:affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28422486</idno><idno type="pmid">28422486</idno><idno type="doi">10.1021/acsami.7b03987</idno><idno type="wicri:Area/Main/Corpus">000561</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000561</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Stable Nanocomposite Based on PEGylated and Silver Nanoparticles Loaded Graphene Oxide for Long-Term Antibacterial Activity.</title><author><name sortKey="Zhao, Rongtao" sort="Zhao, Rongtao" uniqKey="Zhao R" first="Rongtao" last="Zhao">Rongtao Zhao</name><affiliation><nlm:affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Lv, Min" sort="Lv, Min" uniqKey="Lv M" first="Min" last="Lv">Min Lv</name><affiliation><nlm:affiliation>Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Yang" sort="Li, Yang" uniqKey="Li Y" first="Yang" last="Li">Yang Li</name><affiliation><nlm:affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Sun, Mingxuan" sort="Sun, Mingxuan" uniqKey="Sun M" first="Mingxuan" last="Sun">Mingxuan Sun</name><affiliation><nlm:affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Kong, Wen" sort="Kong, Wen" uniqKey="Kong W" first="Wen" last="Kong">Wen Kong</name><affiliation><nlm:affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Lihua" sort="Wang, Lihua" uniqKey="Wang L" first="Lihua" last="Wang">Lihua Wang</name><affiliation><nlm:affiliation>Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Song, Shiping" sort="Song, Shiping" uniqKey="Song S" first="Shiping" last="Song">Shiping Song</name><affiliation><nlm:affiliation>Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Fan, Chunhai" sort="Fan, Chunhai" uniqKey="Fan C" first="Chunhai" last="Fan">Chunhai Fan</name><affiliation><nlm:affiliation>Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Jia, Leili" sort="Jia, Leili" uniqKey="Jia L" first="Leili" last="Jia">Leili Jia</name><affiliation><nlm:affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Qiu, Shaofu" sort="Qiu, Shaofu" uniqKey="Qiu S" first="Shaofu" last="Qiu">Shaofu Qiu</name><affiliation><nlm:affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Sun, Yansong" sort="Sun, Yansong" uniqKey="Sun Y" first="Yansong" last="Sun">Yansong Sun</name><affiliation><nlm:affiliation>Department of Science and Technology, AMMS , Beijing 100850, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Song, Hongbin" sort="Song, Hongbin" uniqKey="Song H" first="Hongbin" last="Song">Hongbin Song</name><affiliation><nlm:affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Hao, Rongzhang" sort="Hao, Rongzhang" uniqKey="Hao R" first="Rongzhang" last="Hao">Rongzhang Hao</name><affiliation><nlm:affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">ACS applied materials & interfaces</title><idno type="eISSN">1944-8252</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-Bacterial Agents (MeSH)</term><term>Escherichia coli (MeSH)</term><term>Graphite (MeSH)</term><term>HeLa Cells (MeSH)</term><term>Humans (MeSH)</term><term>Metal Nanoparticles (MeSH)</term><term>Nanocomposites (MeSH)</term><term>Oxides (MeSH)</term><term>Silver (MeSH)</term><term>Staphylococcus aureus (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Graphite</term><term>Oxides</term><term>Silver</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Escherichia coli</term><term>HeLa Cells</term><term>Humans</term><term>Metal Nanoparticles</term><term>Nanocomposites</term><term>Staphylococcus aureus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The increasing occurrence of antibiotic-resistant pathogens, especially superbugs, is compromising the efficacy of traditional antibiotics. Silver nanoparticles (AgNPs) loaded graphene oxide (GO) nanocomposite (GO-Ag) has drawn great interest as a promising alternative antibacterial material. However, GO-Ag nanocomposite often irreversibly aggregates in physiological solutions, severely influencing its antibacterial capacity and practical application. Herein, a PEGylated and AgNPs loaded GO nanocomposite (GO-PEG-Ag) is synthesized through a facile approach utilizing microwave irradiation, while avoiding extra reducing agents. Through PEGylation, the synthesized GO-PEG-Ag nanocomposite dispersed stably over one month in a series of media and resisted centrifugation at 10 000×g for 5 min, which would benefit effective contact between the nanocomposite and the bacteria. In contrast, GO-Ag aggregated within 1 h of dispersion in physiological solutions. In comparison with GO-Ag, GO-PEG-Ag showed stronger bactericidal capability toward not only normal Gram-negative/positive bacteria such as E. coli and S. aureus (∼100% of E. coli and ∼95.3% of S. aureus reduction by 10 μg/mL nanocomposite for 2.5 h), but also superbugs. Moreover, GO-PEG-Ag showed lower cytotoxicity toward HeLa cells. Importantly, GO-PEG-Ag presented long-term antibacterial effectiveness, remaining ∼95% antibacterial activity after one-week storage in saline solution versus <35% for GO-Ag. The antibacterial mechanisms of GO-PEG-Ag were evidenced as damage to the bacterial structure and production of reactive oxygen species, causing cytoplasm leakage and metabolism decrease. The stable GO-PEG-Ag nanocomposite with powerful and long-term antibacterial capability provides a more practical and effective strategy for fighting superbugs-including pathogen threats in biomedicine and public health.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">28422486</PMID><DateCompleted><Year>2019</Year><Month>01</Month><Day>07</Day></DateCompleted><DateRevised><Year>2019</Year><Month>01</Month><Day>07</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1944-8252</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>18</Issue><PubDate><Year>2017</Year><Month>May</Month><Day>10</Day></PubDate></JournalIssue><Title>ACS applied materials & interfaces</Title><ISOAbbreviation>ACS Appl Mater Interfaces</ISOAbbreviation></Journal><ArticleTitle>Stable Nanocomposite Based on PEGylated and Silver Nanoparticles Loaded Graphene Oxide for Long-Term Antibacterial Activity.</ArticleTitle><Pagination><MedlinePgn>15328-15341</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acsami.7b03987</ELocationID><Abstract><AbstractText>The increasing occurrence of antibiotic-resistant pathogens, especially superbugs, is compromising the efficacy of traditional antibiotics. Silver nanoparticles (AgNPs) loaded graphene oxide (GO) nanocomposite (GO-Ag) has drawn great interest as a promising alternative antibacterial material. However, GO-Ag nanocomposite often irreversibly aggregates in physiological solutions, severely influencing its antibacterial capacity and practical application. Herein, a PEGylated and AgNPs loaded GO nanocomposite (GO-PEG-Ag) is synthesized through a facile approach utilizing microwave irradiation, while avoiding extra reducing agents. Through PEGylation, the synthesized GO-PEG-Ag nanocomposite dispersed stably over one month in a series of media and resisted centrifugation at 10 000×g for 5 min, which would benefit effective contact between the nanocomposite and the bacteria. In contrast, GO-Ag aggregated within 1 h of dispersion in physiological solutions. In comparison with GO-Ag, GO-PEG-Ag showed stronger bactericidal capability toward not only normal Gram-negative/positive bacteria such as E. coli and S. aureus (∼100% of E. coli and ∼95.3% of S. aureus reduction by 10 μg/mL nanocomposite for 2.5 h), but also superbugs. Moreover, GO-PEG-Ag showed lower cytotoxicity toward HeLa cells. Importantly, GO-PEG-Ag presented long-term antibacterial effectiveness, remaining ∼95% antibacterial activity after one-week storage in saline solution versus <35% for GO-Ag. The antibacterial mechanisms of GO-PEG-Ag were evidenced as damage to the bacterial structure and production of reactive oxygen species, causing cytoplasm leakage and metabolism decrease. The stable GO-PEG-Ag nanocomposite with powerful and long-term antibacterial capability provides a more practical and effective strategy for fighting superbugs-including pathogen threats in biomedicine and public health.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Rongtao</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lv</LastName><ForeName>Min</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Mingxuan</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kong</LastName><ForeName>Wen</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Lihua</ForeName><Initials>L</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-6198-7561</Identifier><AffiliationInfo><Affiliation>Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Shiping</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fan</LastName><ForeName>Chunhai</ForeName><Initials>C</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-7171-7338</Identifier><AffiliationInfo><Affiliation>Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jia</LastName><ForeName>Leili</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qiu</LastName><ForeName>Shaofu</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Yansong</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Science and Technology, AMMS , Beijing 100850, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Hongbin</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hao</LastName><ForeName>Rongzhang</ForeName><Initials>R</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-1527-427X</Identifier><AffiliationInfo><Affiliation>Institute of Disease Control and Prevention, AMMS , Beijing 100071, P. R. China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>04</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>ACS Appl Mater Interfaces</MedlineTA><NlmUniqueID>101504991</NlmUniqueID><ISSNLinking>1944-8244</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010087">Oxides</NameOfSubstance></Chemical><Chemical><RegistryNumber>3M4G523W1G</RegistryNumber><NameOfSubstance UI="D012834">Silver</NameOfSubstance></Chemical><Chemical><RegistryNumber>7782-42-5</RegistryNumber><NameOfSubstance UI="D006108">Graphite</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006108" MajorTopicYN="N">Graphite</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006367" MajorTopicYN="N">HeLa Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053768" MajorTopicYN="N">Metal Nanoparticles</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053761" MajorTopicYN="Y">Nanocomposites</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010087" MajorTopicYN="N">Oxides</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012834" MajorTopicYN="N">Silver</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013211" MajorTopicYN="N">Staphylococcus aureus</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">PEGylation</Keyword><Keyword MajorTopicYN="N">antibacterial activity</Keyword><Keyword MajorTopicYN="N">antibiotic resistance</Keyword><Keyword MajorTopicYN="N">graphene oxide</Keyword><Keyword MajorTopicYN="N">long-term effectiveness</Keyword><Keyword MajorTopicYN="N">silver nanoparticles</Keyword><Keyword MajorTopicYN="N">stability</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>4</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>1</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>4</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28422486</ArticleId><ArticleId IdType="doi">10.1021/acsami.7b03987</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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