Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures.
Identifieur interne : 000217 ( PubMed/Corpus ); précédent : 000216; suivant : 000218Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures.
Auteurs : S. Papanikolaou ; I. Chevalot ; M. Komaitis ; I. Marc ; G. AggelisSource :
- Applied microbiology and biotechnology [ 0175-7598 ] ; 2002.
English descriptors
- KwdEn :
- Air Movements, Ammonium Sulfate (metabolism), Animals, Bioreactors (microbiology), Culture Media, Fatty Acids (metabolism), Fermentation, Food Industry, Glycerol (metabolism), Hot Temperature, Hydrogen-Ion Concentration, Industrial Oils, Kinetics, Lipid Metabolism, Lipids (biosynthesis), Oils (metabolism), Stearic Acids (analysis), Stearic Acids (metabolism), Yarrowia (growth & development), Yarrowia (metabolism).
- MESH :
- chemical , analysis : Stearic Acids.
- chemical , biosynthesis : Lipids.
- chemical , metabolism : Ammonium Sulfate, Fatty Acids, Glycerol, Oils, Stearic Acids.
- growth & development : Yarrowia.
- metabolism : Yarrowia.
- microbiology : Bioreactors.
- Air Movements, Animals, Culture Media, Fermentation, Food Industry, Hot Temperature, Hydrogen-Ion Concentration, Industrial Oils, Kinetics, Lipid Metabolism.
Abstract
The growth of an oleaginous strain of Yarrowia lipolytica on an industrial fat composed of saturated free fatty acids (stearin) was studied. Lipid accumulation during primary anabolic growth was critically influenced by the medium pH and the incubation temperature. This process was independent of the nitrogen concentration in the culture medium, but was favored at a high carbon substrate level and at a low aeration rate. At pH 6 and a temperature of 28-33 degrees C, 9-12 g/l of dry biomass was produced, whereas significant quantities of lipids were accumulated inside the yeast cells (0.44-0.54 g of lipid per gram of biomass). The strain showed the tendency to degrade its storage lipids, although significant amounts of substrate fat, rich in stearic acid, remained unconsumed in the culture medium. Y. lipolytica presented a strong fatty acid specificity. The fatty acids C12:0, C14:0, and C16:0 were rapidly incorporated and mainly used for growth needs, while C18:0 was incorporated with reduced rates and was mainly accumulated as storage material. Reserve lipids, principally composed of triacylglycerols (55% w/w of total lipids) and free fatty acids (35% w/w), were rich in stearic acid (80% w/w), while negligible amounts of unsaturated fatty acids were detected. When industrial glycerol was used as co-substrate, together with stearin, unsaturated fatty acid concentration in the reserve lipid increased.
DOI: 10.1007/s00253-001-0897-0
PubMed: 11935181
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Aggelis</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:11935181</idno><idno type="pmid">11935181</idno><idno type="doi">10.1007/s00253-001-0897-0</idno><idno type="wicri:Area/PubMed/Corpus">000217</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000217</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures.</title><author><name sortKey="Papanikolaou, S" sort="Papanikolaou, S" uniqKey="Papanikolaou S" first="S" last="Papanikolaou">S. Papanikolaou</name><affiliation><nlm:affiliation>Laboratoire des Sciences du Génie Chimique-CNRS, ENSIC, UPR 6811, Vandoeuvre-lès-Nancy, France.</nlm:affiliation></affiliation></author><author><name sortKey="Chevalot, I" sort="Chevalot, I" uniqKey="Chevalot I" first="I" last="Chevalot">I. Chevalot</name></author><author><name sortKey="Komaitis, M" sort="Komaitis, M" uniqKey="Komaitis M" first="M" last="Komaitis">M. Komaitis</name></author><author><name sortKey="Marc, I" sort="Marc, I" uniqKey="Marc I" first="I" last="Marc">I. Marc</name></author><author><name sortKey="Aggelis, G" sort="Aggelis, G" uniqKey="Aggelis G" first="G" last="Aggelis">G. Aggelis</name></author></analytic><series><title level="j">Applied microbiology and biotechnology</title><idno type="ISSN">0175-7598</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Air Movements</term><term>Ammonium Sulfate (metabolism)</term><term>Animals</term><term>Bioreactors (microbiology)</term><term>Culture Media</term><term>Fatty Acids (metabolism)</term><term>Fermentation</term><term>Food Industry</term><term>Glycerol (metabolism)</term><term>Hot Temperature</term><term>Hydrogen-Ion Concentration</term><term>Industrial Oils</term><term>Kinetics</term><term>Lipid Metabolism</term><term>Lipids (biosynthesis)</term><term>Oils (metabolism)</term><term>Stearic Acids (analysis)</term><term>Stearic Acids (metabolism)</term><term>Yarrowia (growth & development)</term><term>Yarrowia (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Stearic Acids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Lipids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ammonium Sulfate</term><term>Fatty Acids</term><term>Glycerol</term><term>Oils</term><term>Stearic Acids</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Yarrowia</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Yarrowia</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Bioreactors</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Air Movements</term><term>Animals</term><term>Culture Media</term><term>Fermentation</term><term>Food Industry</term><term>Hot Temperature</term><term>Hydrogen-Ion Concentration</term><term>Industrial Oils</term><term>Kinetics</term><term>Lipid Metabolism</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The growth of an oleaginous strain of Yarrowia lipolytica on an industrial fat composed of saturated free fatty acids (stearin) was studied. Lipid accumulation during primary anabolic growth was critically influenced by the medium pH and the incubation temperature. This process was independent of the nitrogen concentration in the culture medium, but was favored at a high carbon substrate level and at a low aeration rate. At pH 6 and a temperature of 28-33 degrees C, 9-12 g/l of dry biomass was produced, whereas significant quantities of lipids were accumulated inside the yeast cells (0.44-0.54 g of lipid per gram of biomass). The strain showed the tendency to degrade its storage lipids, although significant amounts of substrate fat, rich in stearic acid, remained unconsumed in the culture medium. Y. lipolytica presented a strong fatty acid specificity. The fatty acids C12:0, C14:0, and C16:0 were rapidly incorporated and mainly used for growth needs, while C18:0 was incorporated with reduced rates and was mainly accumulated as storage material. Reserve lipids, principally composed of triacylglycerols (55% w/w of total lipids) and free fatty acids (35% w/w), were rich in stearic acid (80% w/w), while negligible amounts of unsaturated fatty acids were detected. When industrial glycerol was used as co-substrate, together with stearin, unsaturated fatty acid concentration in the reserve lipid increased.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">11935181</PMID><DateCreated><Year>2002</Year><Month>04</Month><Day>08</Day></DateCreated><DateCompleted><Year>2002</Year><Month>11</Month><Day>14</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0175-7598</ISSN><JournalIssue CitedMedium="Print"><Volume>58</Volume><Issue>3</Issue><PubDate><Year>2002</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl. Microbiol. Biotechnol.</ISOAbbreviation></Journal><ArticleTitle>Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures.</ArticleTitle><Pagination><MedlinePgn>308-12</MedlinePgn></Pagination><Abstract><AbstractText>The growth of an oleaginous strain of Yarrowia lipolytica on an industrial fat composed of saturated free fatty acids (stearin) was studied. Lipid accumulation during primary anabolic growth was critically influenced by the medium pH and the incubation temperature. This process was independent of the nitrogen concentration in the culture medium, but was favored at a high carbon substrate level and at a low aeration rate. At pH 6 and a temperature of 28-33 degrees C, 9-12 g/l of dry biomass was produced, whereas significant quantities of lipids were accumulated inside the yeast cells (0.44-0.54 g of lipid per gram of biomass). The strain showed the tendency to degrade its storage lipids, although significant amounts of substrate fat, rich in stearic acid, remained unconsumed in the culture medium. Y. lipolytica presented a strong fatty acid specificity. The fatty acids C12:0, C14:0, and C16:0 were rapidly incorporated and mainly used for growth needs, while C18:0 was incorporated with reduced rates and was mainly accumulated as storage material. Reserve lipids, principally composed of triacylglycerols (55% w/w of total lipids) and free fatty acids (35% w/w), were rich in stearic acid (80% w/w), while negligible amounts of unsaturated fatty acids were detected. When industrial glycerol was used as co-substrate, together with stearin, unsaturated fatty acid concentration in the reserve lipid increased.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Papanikolaou</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratoire des Sciences du Génie Chimique-CNRS, ENSIC, UPR 6811, Vandoeuvre-lès-Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chevalot</LastName><ForeName>I</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Komaitis</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Marc</LastName><ForeName>I</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Aggelis</LastName><ForeName>G</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2001</Year><Month>12</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Appl Microbiol Biotechnol</MedlineTA><NlmUniqueID>8406612</NlmUniqueID><ISSNLinking>0175-7598</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005227">Fatty Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008055">Lipids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009821">Oils</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013229">Stearic Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>PDC6A3C0OX</RegistryNumber><NameOfSubstance UI="D005990">Glycerol</NameOfSubstance></Chemical><Chemical><RegistryNumber>SU46BAM238</RegistryNumber><NameOfSubstance UI="D000645">Ammonium Sulfate</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000392">Air Movements</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000645">Ammonium Sulfate</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000818">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D019149">Bioreactors</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000382">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D003470">Culture Media</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D005227">Fatty Acids</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D005285">Fermentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D019649">Food Industry</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D005990">Glycerol</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D006358">Hot Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D006863">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y" UI="D007219">Industrial Oils</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D007700">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D050356">Lipid Metabolism</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D008055">Lipids</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000096">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D009821">Oils</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D013229">Stearic Acids</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000032">analysis</QualifierName><QualifierName MajorTopicYN="N" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D025062">Yarrowia</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000254">growth & development</QualifierName><QualifierName MajorTopicYN="Y" UI="Q000378">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2002</Year><Month>4</Month><Day>6</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2002</Year><Month>11</Month><Day>26</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="received"><Year>2001</Year><Month>Sep</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2001</Year><Month>Oct</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2001</Year><Month>Nov</Month><Day>2</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2001</Year><Month>Dec</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2002</Year><Month>4</Month><Day>6</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId 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