Broad-spectrum disease resistance to necrotrophic and biotrophic pathogens in transgenic carrots (Daucus carota L.) expressing an Arabidopsis NPR1 gene.
Identifieur interne : 000120 ( PubMed/Checkpoint ); précédent : 000119; suivant : 000121Broad-spectrum disease resistance to necrotrophic and biotrophic pathogens in transgenic carrots (Daucus carota L.) expressing an Arabidopsis NPR1 gene.
Auteurs : Owen Wally [Canada] ; Jayaraman Jayaraj ; Zamir K. PunjaSource :
- Planta [ 1432-2048 ] ; 2009.
Descripteurs français
- KwdFr :
- ARN messager (génétique), ARN messager (métabolisme), Alternaria (physiologie), Arabidopsis (génétique), Botrytis (physiologie), Champignons (physiologie), Daucus carota (génétique), Daucus carota (immunologie), Daucus carota (microbiologie), Facteurs temps, Gènes de plante (génétique), Immunité innée (immunologie), Maladies des plantes (génétique), Maladies des plantes (immunologie), Maladies des plantes (microbiologie), Protéines d'Arabidopsis (génétique), Protéines d'Arabidopsis (métabolisme), Régulation de l'expression des gènes végétaux, Transformation génétique, Végétaux génétiquement modifiés.
- MESH :
- génétique : ARN messager, Arabidopsis, Daucus carota, Gènes de plante, Maladies des plantes, Protéines d'Arabidopsis.
- immunologie : Daucus carota, Immunité innée, Maladies des plantes.
- microbiologie : Daucus carota, Maladies des plantes.
- métabolisme : ARN messager, Protéines d'Arabidopsis.
- physiologie : Alternaria, Botrytis, Champignons.
- Facteurs temps, Régulation de l'expression des gènes végétaux, Transformation génétique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Alternaria (physiology), Arabidopsis (genetics), Arabidopsis Proteins (genetics), Arabidopsis Proteins (metabolism), Botrytis (physiology), Daucus carota (genetics), Daucus carota (immunology), Daucus carota (microbiology), Fungi (physiology), Gene Expression Regulation, Plant, Genes, Plant (genetics), Immunity, Innate (immunology), Plant Diseases (genetics), Plant Diseases (immunology), Plant Diseases (microbiology), Plants, Genetically Modified, RNA, Messenger (genetics), RNA, Messenger (metabolism), Time Factors, Transformation, Genetic.
- MESH :
- chemical , genetics : Arabidopsis Proteins, RNA, Messenger.
- genetics : Arabidopsis, Daucus carota, Genes, Plant, Plant Diseases.
- immunology : Daucus carota, Immunity, Innate, Plant Diseases.
- chemical , metabolism : Arabidopsis Proteins, RNA, Messenger.
- microbiology : Daucus carota, Plant Diseases.
- physiology : Alternaria, Botrytis, Fungi.
- Gene Expression Regulation, Plant, Plants, Genetically Modified, Time Factors, Transformation, Genetic.
Abstract
The development of transgenic plants highly resistant to a range of pathogens using traditional signal gene expression strategies has been largely ineffective. Modification of systemic acquired resistance (SAR) through the overexpression of a controlling gene such as NPR1 (non-expressor of PR genes) offers an attractive alternative for augmenting the plants innate defense system. The Arabidopsis (At) NPR1 gene was successfully introduced into 'Nantes Coreless' carrot under control of a CaMV 35S promoter and two independent transgenic lines (NPR1-I and NPR1-XI) were identified by Southern and Northern blot hybridization. Both lines were phenotypically normal compared with non-transformed carrots. Northern analysis did not indicate constitutive or spontaneous induction in carrot cultures of SAR-related genes (DcPR-1, 2, 4, 5 or DcPAL). The duration and intensity of expression of DcPR-1, 2 and 5 genes were greatly increased compared with controls when the lines were treated with purified cell wall fragments of Sclerotinia sclerotiorum as well as with 2,6-dichloroisonicotinic acid. The two lines were challenged with the necrotrophic pathogens Botrytis cinerea, Alternaria radicina and S. sclerotiorum on the foliage and A. radicina on the taproots. Both lines exhibited 35-50% reduction in disease symptoms on the foliage and roots when compared with non-transgenic controls. Leaves challenged with the biotrophic pathogen Erysiphe heraclei or the bacterial pathogen Xanthomonas hortorum exhibited 90 and 80% reduction in disease development on the transgenic lines, respectively. The overexpression of the SAR controlling master switch in carrot tissues offers the ability to control a wide range of different pathogens, for which there is currently little genetic resistance available.
DOI: 10.1007/s00425-009-1031-2
PubMed: 19859731
Affiliations:
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Punja</name></author></analytic><series><title level="j">Planta</title><idno type="eISSN">1432-2048</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alternaria (physiology)</term><term>Arabidopsis (genetics)</term><term>Arabidopsis Proteins (genetics)</term><term>Arabidopsis Proteins (metabolism)</term><term>Botrytis (physiology)</term><term>Daucus carota (genetics)</term><term>Daucus carota (immunology)</term><term>Daucus carota (microbiology)</term><term>Fungi (physiology)</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant (genetics)</term><term>Immunity, Innate (immunology)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (immunology)</term><term>Plant Diseases (microbiology)</term><term>Plants, Genetically Modified</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>Time Factors</term><term>Transformation, Genetic</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>ARN messager (métabolisme)</term><term>Alternaria (physiologie)</term><term>Arabidopsis (génétique)</term><term>Botrytis (physiologie)</term><term>Champignons (physiologie)</term><term>Daucus carota (génétique)</term><term>Daucus carota (immunologie)</term><term>Daucus carota (microbiologie)</term><term>Facteurs temps</term><term>Gènes de plante (génétique)</term><term>Immunité innée (immunologie)</term><term>Maladies des plantes (génétique)</term><term>Maladies des plantes (immunologie)</term><term>Maladies des plantes (microbiologie)</term><term>Protéines d'Arabidopsis (génétique)</term><term>Protéines d'Arabidopsis (métabolisme)</term><term>Régulation de l'expression des gènes végétaux</term><term>Transformation génétique</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Arabidopsis Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Daucus carota</term><term>Genes, Plant</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Arabidopsis</term><term>Daucus carota</term><term>Gènes de plante</term><term>Maladies des plantes</term><term>Protéines d'Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Daucus carota</term><term>Immunité innée</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Daucus carota</term><term>Immunity, Innate</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Arabidopsis Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Daucus carota</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Daucus carota</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Protéines d'Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Alternaria</term><term>Botrytis</term><term>Champignons</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Alternaria</term><term>Botrytis</term><term>Fungi</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Plants, Genetically Modified</term><term>Time Factors</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Facteurs temps</term><term>Régulation de l'expression des gènes végétaux</term><term>Transformation génétique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The development of transgenic plants highly resistant to a range of pathogens using traditional signal gene expression strategies has been largely ineffective. Modification of systemic acquired resistance (SAR) through the overexpression of a controlling gene such as NPR1 (non-expressor of PR genes) offers an attractive alternative for augmenting the plants innate defense system. The Arabidopsis (At) NPR1 gene was successfully introduced into 'Nantes Coreless' carrot under control of a CaMV 35S promoter and two independent transgenic lines (NPR1-I and NPR1-XI) were identified by Southern and Northern blot hybridization. Both lines were phenotypically normal compared with non-transformed carrots. Northern analysis did not indicate constitutive or spontaneous induction in carrot cultures of SAR-related genes (DcPR-1, 2, 4, 5 or DcPAL). The duration and intensity of expression of DcPR-1, 2 and 5 genes were greatly increased compared with controls when the lines were treated with purified cell wall fragments of Sclerotinia sclerotiorum as well as with 2,6-dichloroisonicotinic acid. The two lines were challenged with the necrotrophic pathogens Botrytis cinerea, Alternaria radicina and S. sclerotiorum on the foliage and A. radicina on the taproots. Both lines exhibited 35-50% reduction in disease symptoms on the foliage and roots when compared with non-transgenic controls. Leaves challenged with the biotrophic pathogen Erysiphe heraclei or the bacterial pathogen Xanthomonas hortorum exhibited 90 and 80% reduction in disease development on the transgenic lines, respectively. The overexpression of the SAR controlling master switch in carrot tissues offers the ability to control a wide range of different pathogens, for which there is currently little genetic resistance available.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">19859731</PMID><DateCreated><Year>2010</Year><Month>01</Month><Day>21</Day></DateCreated><DateCompleted><Year>2010</Year><Month>03</Month><Day>16</Day></DateCompleted><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-2048</ISSN><JournalIssue CitedMedium="Internet"><Volume>231</Volume><Issue>1</Issue><PubDate><Year>2009</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Planta</Title><ISOAbbreviation>Planta</ISOAbbreviation></Journal><ArticleTitle>Broad-spectrum disease resistance to necrotrophic and biotrophic pathogens in transgenic carrots (Daucus carota L.) expressing an Arabidopsis NPR1 gene.</ArticleTitle><Pagination><MedlinePgn>131-41</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00425-009-1031-2</ELocationID><Abstract><AbstractText>The development of transgenic plants highly resistant to a range of pathogens using traditional signal gene expression strategies has been largely ineffective. Modification of systemic acquired resistance (SAR) through the overexpression of a controlling gene such as NPR1 (non-expressor of PR genes) offers an attractive alternative for augmenting the plants innate defense system. The Arabidopsis (At) NPR1 gene was successfully introduced into 'Nantes Coreless' carrot under control of a CaMV 35S promoter and two independent transgenic lines (NPR1-I and NPR1-XI) were identified by Southern and Northern blot hybridization. Both lines were phenotypically normal compared with non-transformed carrots. Northern analysis did not indicate constitutive or spontaneous induction in carrot cultures of SAR-related genes (DcPR-1, 2, 4, 5 or DcPAL). The duration and intensity of expression of DcPR-1, 2 and 5 genes were greatly increased compared with controls when the lines were treated with purified cell wall fragments of Sclerotinia sclerotiorum as well as with 2,6-dichloroisonicotinic acid. The two lines were challenged with the necrotrophic pathogens Botrytis cinerea, Alternaria radicina and S. sclerotiorum on the foliage and A. radicina on the taproots. Both lines exhibited 35-50% reduction in disease symptoms on the foliage and roots when compared with non-transgenic controls. Leaves challenged with the biotrophic pathogen Erysiphe heraclei or the bacterial pathogen Xanthomonas hortorum exhibited 90 and 80% reduction in disease development on the transgenic lines, respectively. The overexpression of the SAR controlling master switch in carrot tissues offers the ability to control a wide range of different pathogens, for which there is currently little genetic resistance available.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wally</LastName><ForeName>Owen</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC, V5A 1S6, Canada. owenw@sfu.ca</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jayaraj</LastName><ForeName>Jayaraman</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Punja</LastName><ForeName>Zamir K</ForeName><Initials>ZK</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>10</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Planta</MedlineTA><NlmUniqueID>1250576</NlmUniqueID><ISSNLinking>0032-0935</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029681">Arabidopsis Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C105496">NPR1 protein, Arabidopsis</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000528">Alternaria</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000502">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D017360">Arabidopsis</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000235">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D029681">Arabidopsis Proteins</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000235">genetics</QualifierName><QualifierName MajorTopicYN="N" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D020171">Botrytis</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000502">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D018552">Daucus carota</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000235">genetics</QualifierName><QualifierName MajorTopicYN="N" UI="Q000276">immunology</QualifierName><QualifierName MajorTopicYN="Y" UI="Q000382">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D005658">Fungi</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000502">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D018506">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D017343">Genes, Plant</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000235">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D007113">Immunity, Innate</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000276">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D010935">Plant Diseases</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000235">genetics</QualifierName><QualifierName MajorTopicYN="Y" UI="Q000276">immunology</QualifierName><QualifierName MajorTopicYN="Y" UI="Q000382">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D030821">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D012333">RNA, Messenger</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000235">genetics</QualifierName><QualifierName MajorTopicYN="N" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D013997">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D014170">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>7</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>9</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2009</Year><Month>10</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>10</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>10</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>3</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1007/s00425-009-1031-2</ArticleId><ArticleId IdType="pubmed">19859731</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Jayaraj, Jayaraman" sort="Jayaraj, Jayaraman" uniqKey="Jayaraj J" first="Jayaraman" last="Jayaraj">Jayaraman Jayaraj</name><name sortKey="Punja, Zamir K" sort="Punja, Zamir K" uniqKey="Punja Z" first="Zamir K" last="Punja">Zamir K. Punja</name></noCountry><country name="Canada"><noRegion><name sortKey="Wally, Owen" sort="Wally, Owen" uniqKey="Wally O" first="Owen" last="Wally">Owen Wally</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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