Hydrophobically modified alginate hydrogels as protein carriers with specific controlled release properties.
Identifieur interne : 001788 ( Main/Merge ); précédent : 001787; suivant : 001789Hydrophobically modified alginate hydrogels as protein carriers with specific controlled release properties.
Auteurs : M. Leonard [France] ; M Rastello De Boisseson ; P. Hubert ; F. Dalençon ; Édith Dellacherie [France]Source :
- Journal of controlled release : official journal of the Controlled Release Society [ 0168-3659 ] ; 2004.
Descripteurs français
- KwdFr :
- Alginates (), Animaux, Calendrier vaccinal, Chimie physique, Concentration en ions d'hydrogène, Fluorescéine-5-isothiocyanate, Helicobacter pylori (immunologie), Hydrogels (), Hémoglobines (immunologie), Immunisation, Immunoglobuline G (biosynthèse), Microsphères, Protéines (administration et posologie), Protéines (pharmacocinétique), Préparation de médicament, Préparations à action retardée, Souris, Sérumalbumine bovine (), Taille de particule, Urease (immunologie), Vaccins antibactériens (immunologie), Vecteurs de médicaments ().
- MESH :
- administration et posologie : Protéines.
- biosynthèse : Immunoglobuline G.
- immunologie : Helicobacter pylori, Hémoglobines, Urease, Vaccins antibactériens.
- pharmacocinétique : Protéines.
- Alginates, Animaux, Calendrier vaccinal, Chimie physique, Concentration en ions d'hydrogène, Fluorescéine-5-isothiocyanate, Hydrogels, Immunisation, Microsphères, Préparation de médicament, Préparations à action retardée, Souris, Sérumalbumine bovine, Taille de particule, Vecteurs de médicaments.
English descriptors
- KwdEn :
- Alginates (chemistry), Animals, Bacterial Vaccines (immunology), Chemistry, Physical, Delayed-Action Preparations, Drug Carriers (chemistry), Drug Compounding, Fluorescein-5-isothiocyanate, Helicobacter pylori (immunology), Hemoglobins (immunology), Hydrogels (chemistry), Hydrogen-Ion Concentration, Immunization, Immunization Schedule, Immunoglobulin G (biosynthesis), Mice, Microspheres, Particle Size, Physicochemical Phenomena, Proteins (administration & dosage), Proteins (pharmacokinetics), Serum Albumin, Bovine (chemistry), Urease (immunology).
- MESH :
- chemical , administration & dosage : Proteins.
- chemical , biosynthesis : Immunoglobulin G.
- chemical , chemistry : Alginates, Drug Carriers, Hydrogels, Serum Albumin, Bovine.
- chemical , immunology : Bacterial Vaccines, Hemoglobins, Urease.
- immunology : Helicobacter pylori.
- chemical , pharmacokinetics : Proteins.
- Animals, Chemistry, Physical, Delayed-Action Preparations, Drug Compounding, Fluorescein-5-isothiocyanate, Hydrogen-Ion Concentration, Immunization, Immunization Schedule, Mice, Microspheres, Particle Size, Physicochemical Phenomena.
Abstract
Amphiphilic derivatives of sodium alginate, prepared by chemical covalent binding of long alkyl chains onto the polysaccharide backbone via ester functions, form strong hydrogels in aqueous solutions. The shear-thinning and thixotropic behaviors of these hydrogels have been exploited to prepare particles (millimetric beads or microparticles) by dispersion in sodium chloride solutions. This all-aqueous procedure was used for the encapsulation of model proteins, such as bovine serum albumin (BSA) and human hemoglobin (Hb), or of a vaccine protein (Helicobacter pylori (H. pylori) urease). In all cases, the encapsulation yields were very high (70-100%). No release of model proteins was observed in water within several days, in contrast with protein-loaded calcium alginate particles, which exhibit an important release within only a few hours. The controlled release of proteins can, however, be achieved by inducing the dissociation of the physical hydrophobic network. This dissociation has been obtained either by addition of surfactants, acting as disrupting agents of intermolecular hydrophobic junctions, or of esterases such as lipases, which hydrolyze the ester bond between alkyl chains and the polysaccharide backbone. The level of immunization against H. pylori infection in mice, induced by encapsulated urease administrated by either systemic or mucosal routes, was also assessed.
DOI: 10.1016/j.jconrel.2004.05.009
PubMed: 15312995
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pubmed:15312995Le document en format XML
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Leonard</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Chimie Physique Macromoléculaire, Groupe ENSIC, BP 451, UMR CNRS-INPL 7568, 54001 Nancy Cedex, France. Michele.Leonard@ensic.inpl-nancy.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Chimie Physique Macromoléculaire, Groupe ENSIC, BP 451, UMR CNRS-INPL 7568, 54001 Nancy Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Nancy</settlement></placeName></affiliation></author><author><name sortKey="De Boisseson, M Rastello" sort="De Boisseson, M Rastello" uniqKey="De Boisseson M" first="M Rastello" last="De Boisseson">M Rastello De Boisseson</name></author><author><name sortKey="Hubert, P" sort="Hubert, P" uniqKey="Hubert P" first="P" last="Hubert">P. Hubert</name></author><author><name sortKey="Dalencon, F" sort="Dalencon, F" uniqKey="Dalencon F" first="F" last="Dalençon">F. Dalençon</name></author><author><name sortKey="Dellacherie, E" sort="Dellacherie, E" uniqKey="Dellacherie E" first="E" last="Dellacherie">Édith Dellacherie</name><affiliation><country>France</country><placeName><settlement type="city">Nancy</settlement><region type="region" nuts="2">Grand Est</region><region type="region" nuts="2">Lorraine (région)</region></placeName><orgName type="laboratoire" n="5">Laboratoire réactions et génie des procédés</orgName><orgName type="university">Université de Lorraine</orgName><orgName type="institution">Centre national de la recherche scientifique</orgName></affiliation></author></analytic><series><title level="j">Journal of controlled release : official journal of the Controlled Release Society</title><idno type="ISSN">0168-3659</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alginates (chemistry)</term><term>Animals</term><term>Bacterial Vaccines (immunology)</term><term>Chemistry, Physical</term><term>Delayed-Action Preparations</term><term>Drug Carriers (chemistry)</term><term>Drug Compounding</term><term>Fluorescein-5-isothiocyanate</term><term>Helicobacter pylori (immunology)</term><term>Hemoglobins (immunology)</term><term>Hydrogels (chemistry)</term><term>Hydrogen-Ion Concentration</term><term>Immunization</term><term>Immunization Schedule</term><term>Immunoglobulin G (biosynthesis)</term><term>Mice</term><term>Microspheres</term><term>Particle Size</term><term>Physicochemical Phenomena</term><term>Proteins (administration & dosage)</term><term>Proteins (pharmacokinetics)</term><term>Serum Albumin, Bovine (chemistry)</term><term>Urease (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alginates ()</term><term>Animaux</term><term>Calendrier vaccinal</term><term>Chimie physique</term><term>Concentration en ions d'hydrogène</term><term>Fluorescéine-5-isothiocyanate</term><term>Helicobacter pylori (immunologie)</term><term>Hydrogels ()</term><term>Hémoglobines (immunologie)</term><term>Immunisation</term><term>Immunoglobuline G (biosynthèse)</term><term>Microsphères</term><term>Protéines (administration et posologie)</term><term>Protéines (pharmacocinétique)</term><term>Préparation de médicament</term><term>Préparations à action retardée</term><term>Souris</term><term>Sérumalbumine bovine ()</term><term>Taille de particule</term><term>Urease (immunologie)</term><term>Vaccins antibactériens (immunologie)</term><term>Vecteurs de médicaments ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Alginates</term><term>Drug Carriers</term><term>Hydrogels</term><term>Serum Albumin, Bovine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Bacterial Vaccines</term><term>Hemoglobins</term><term>Urease</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Protéines</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Helicobacter pylori</term><term>Hémoglobines</term><term>Urease</term><term>Vaccins antibactériens</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Helicobacter pylori</term></keywords><keywords scheme="MESH" qualifier="pharmacocinétique" xml:lang="fr"><term>Protéines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Proteins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chemistry, Physical</term><term>Delayed-Action Preparations</term><term>Drug Compounding</term><term>Fluorescein-5-isothiocyanate</term><term>Hydrogen-Ion Concentration</term><term>Immunization</term><term>Immunization Schedule</term><term>Mice</term><term>Microspheres</term><term>Particle Size</term><term>Physicochemical Phenomena</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alginates</term><term>Animaux</term><term>Calendrier vaccinal</term><term>Chimie physique</term><term>Concentration en ions d'hydrogène</term><term>Fluorescéine-5-isothiocyanate</term><term>Hydrogels</term><term>Immunisation</term><term>Microsphères</term><term>Préparation de médicament</term><term>Préparations à action retardée</term><term>Souris</term><term>Sérumalbumine bovine</term><term>Taille de particule</term><term>Vecteurs de médicaments</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Amphiphilic derivatives of sodium alginate, prepared by chemical covalent binding of long alkyl chains onto the polysaccharide backbone via ester functions, form strong hydrogels in aqueous solutions. The shear-thinning and thixotropic behaviors of these hydrogels have been exploited to prepare particles (millimetric beads or microparticles) by dispersion in sodium chloride solutions. This all-aqueous procedure was used for the encapsulation of model proteins, such as bovine serum albumin (BSA) and human hemoglobin (Hb), or of a vaccine protein (Helicobacter pylori (H. pylori) urease). In all cases, the encapsulation yields were very high (70-100%). No release of model proteins was observed in water within several days, in contrast with protein-loaded calcium alginate particles, which exhibit an important release within only a few hours. The controlled release of proteins can, however, be achieved by inducing the dissociation of the physical hydrophobic network. This dissociation has been obtained either by addition of surfactants, acting as disrupting agents of intermolecular hydrophobic junctions, or of esterases such as lipases, which hydrolyze the ester bond between alkyl chains and the polysaccharide backbone. The level of immunization against H. pylori infection in mice, induced by encapsulated urease administrated by either systemic or mucosal routes, was also assessed.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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