LrgpV1, Main, Merge, bibRecord, 000929

Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal.

Identifieur interne : 000929 ( Main/Merge ); précédent : 000928; suivant : 000930

Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal.

Auteurs : Claudia Nioi [France] ; Romain Kapel ; Emmanuel Rondags ; Ivan Marc

Source :

Food chemistry [ 0308-8146 ] ; 2012.

RBID : pubmed:23442668

Descripteurs français

KwdFr :
- Antifongiques (), Antifongiques (isolement et purification), Antifongiques (pharmacologie), Brassica rapa (), Chromatographie sur gel, Extraits de plantes (), Extraits de plantes (isolement et purification), Extraits de plantes (pharmacologie), Fusarium ().
MESH :
- isolement et purification : Antifongiques, Extraits de plantes.
- pharmacologie : Antifongiques, Extraits de plantes.
- Antifongiques, Brassica rapa, Chromatographie sur gel, Extraits de plantes, Fusarium.

English descriptors

KwdEn :
- Antifungal Agents (chemistry), Antifungal Agents (isolation & purification), Antifungal Agents (pharmacology), Brassica rapa (chemistry), Chromatography, Gel, Fusarium (drug effects), Plant Extracts (chemistry), Plant Extracts (isolation & purification), Plant Extracts (pharmacology).
MESH :
- chemical , chemistry : Antifungal Agents, Plant Extracts.
- chemical , isolation & purification : Antifungal Agents, Plant Extracts.
- chemical , pharmacology : Antifungal Agents, Plant Extracts.
- chemistry : Brassica rapa.
- drug effects : Fusarium.
- Chromatography, Gel.
mix :
- Antifungal properties, BRASSICA-JUNCEA, CALMODULIN ANTAGONISTS, CANOLA PROTEINS, DEPENDENT PROTEIN-KINASE, FUNCTIONAL-PROPERTIES, Fusarium langsethiae, HYDROLYSIS, LARGE CHAINS, Napins, PURIFICATION, Protein extraction process, Rapeseed meal, STABILITY, SUBUNIT, Selectivity.

Abstract

This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC(50)=70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.

Url:

https://hal.archives-ouvertes.fr/hal-00777695

DOI: 10.1016/j.foodchem.2012.04.017
PubMed: 23442668

Links to Exploration step

pubmed:23442668

Le document en format XML

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<front><div type="abstract" xml:lang="en">This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC(50)=70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.</div>
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Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal.

Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal.

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