Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal.
Identifieur interne : 000261 ( Ncbi/Checkpoint ); précédent : 000260; suivant : 000262Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal.
Auteurs : Claudia Nioi [France] ; Romain Kapel ; Emmanuel Rondags ; Ivan MarcSource :
- Food chemistry [ 0308-8146 ] ; 2012.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Antifongiques, Extraits de plantes.
- pharmacologie : Antifongiques, Extraits de plantes.
- Antifongiques, Brassica rapa, Chromatographie sur gel, Extraits de plantes, Fusarium.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Antifungal Agents, Plant Extracts.
- chemical , isolation & purification : Antifungal Agents, Plant Extracts.
- chemical , pharmacology : Antifungal Agents, Plant Extracts.
- chemistry : Brassica rapa.
- drug effects : Fusarium.
- Chromatography, Gel.
Abstract
This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC(50)=70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.
DOI: 10.1016/j.foodchem.2012.04.017
PubMed: 23442668
Affiliations:
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pubmed:23442668Le document en format XML
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first="Romain" last="Kapel">Romain Kapel</name></author><author><name sortKey="Rondags, Emmanuel" sort="Rondags, Emmanuel" uniqKey="Rondags E" first="Emmanuel" last="Rondags">Emmanuel Rondags</name></author><author><name sortKey="Marc, Ivan" sort="Marc, Ivan" uniqKey="Marc I" first="Ivan" last="Marc">Ivan Marc</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="doi">10.1016/j.foodchem.2012.04.017</idno><idno type="RBID">pubmed:23442668</idno><idno type="pmid">23442668</idno><idno type="wicri:Area/PubMed/Corpus">000061</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000061</idno><idno type="wicri:Area/PubMed/Curation">000061</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000061</idno><idno type="wicri:Area/PubMed/Checkpoint">000061</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000061</idno><idno 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type="city">Vandœuvre-lès-Nancy</settlement></placeName></affiliation></author><author><name sortKey="Kapel, Romain" sort="Kapel, Romain" uniqKey="Kapel R" first="Romain" last="Kapel">Romain Kapel</name></author><author><name sortKey="Rondags, Emmanuel" sort="Rondags, Emmanuel" uniqKey="Rondags E" first="Emmanuel" last="Rondags">Emmanuel Rondags</name></author><author><name sortKey="Marc, Ivan" sort="Marc, Ivan" uniqKey="Marc I" first="Ivan" last="Marc">Ivan Marc</name></author></analytic><series><title level="j">Food chemistry</title><idno type="ISSN">0308-8146</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antifungal Agents (chemistry)</term><term>Antifungal Agents (isolation & purification)</term><term>Antifungal Agents (pharmacology)</term><term>Brassica rapa (chemistry)</term><term>Chromatography, Gel</term><term>Fusarium (drug effects)</term><term>Plant Extracts (chemistry)</term><term>Plant Extracts (isolation & purification)</term><term>Plant Extracts (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antifongiques ()</term><term>Antifongiques (isolement et purification)</term><term>Antifongiques (pharmacologie)</term><term>Brassica rapa ()</term><term>Chromatographie sur gel</term><term>Extraits de plantes ()</term><term>Extraits de plantes (isolement et purification)</term><term>Extraits de plantes (pharmacologie)</term><term>Fusarium ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antifungal Agents</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antifungal Agents</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antifungal Agents</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Brassica rapa</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antifongiques</term><term>Extraits de plantes</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antifongiques</term><term>Extraits de plantes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, Gel</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Antifongiques</term><term>Brassica rapa</term><term>Chromatographie sur gel</term><term>Extraits de plantes</term><term>Fusarium</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature. Under these conditions the extraction is highly selective, allowing a simple purification process (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified napins. These napins possessed significant anti-Fusarium activity (IC(50)=70 μM) and a compact secondary structure rich in α-helix, which may explain this bioactivity.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Vandœuvre-lès-Nancy</li></settlement></list><tree><noCountry><name sortKey="Kapel, Romain" sort="Kapel, Romain" uniqKey="Kapel R" first="Romain" last="Kapel">Romain Kapel</name><name sortKey="Marc, Ivan" sort="Marc, Ivan" uniqKey="Marc I" first="Ivan" last="Marc">Ivan Marc</name><name sortKey="Rondags, Emmanuel" sort="Rondags, Emmanuel" uniqKey="Rondags E" first="Emmanuel" last="Rondags">Emmanuel Rondags</name></noCountry><country name="France"><region name="Grand Est"><name sortKey="Nioi, Claudia" sort="Nioi, Claudia" uniqKey="Nioi C" first="Claudia" last="Nioi">Claudia Nioi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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