Bioproduction and anticancer activity of biosurfactant produced by the dematiaceous fungus Exophiala dermatitidis SK80.
Identifieur interne : 000D75 ( Main/Exploration ); précédent : 000D74; suivant : 000D76Bioproduction and anticancer activity of biosurfactant produced by the dematiaceous fungus Exophiala dermatitidis SK80.
Auteurs : Paramaporn Chiewpattanakul [France] ; Sirinet Phonnok ; Alain Durand ; Emmanuelle Marie ; Benjamas Wongsatayanon ThanomsubSource :
- Journal of microbiology and biotechnology [ 1738-8872 ] ; 2010.
Descripteurs français
- KwdFr :
- Antibiotiques antinéoplasiques (), Antibiotiques antinéoplasiques (isolement et purification), Antibiotiques antinéoplasiques (métabolisme), Antibiotiques antinéoplasiques (pharmacologie), Antinéoplasiques (), Antinéoplasiques (isolement et purification), Antinéoplasiques (métabolisme), Antinéoplasiques (pharmacologie), Chromatographie en phase liquide, Exophiala (), Exophiala (génétique), Exophiala (isolement et purification), Exophiala (métabolisme), Gel de silice, Huiles végétales (métabolisme), Humains, Lignée cellulaire tumorale, Microbiologie du sol, Milieux de culture (), Spectroscopie par résonance magnétique, Structure moléculaire, Tensioactifs (), Tensioactifs (isolement et purification), Tensioactifs (métabolisme), Tensioactifs (pharmacologie).
- MESH :
- génétique : Exophiala.
- isolement et purification : Antibiotiques antinéoplasiques, Antinéoplasiques, Exophiala, Tensioactifs.
- métabolisme : Antibiotiques antinéoplasiques, Antinéoplasiques, Exophiala, Huiles végétales, Tensioactifs.
- pharmacologie : Antibiotiques antinéoplasiques, Antinéoplasiques, Tensioactifs.
- Antibiotiques antinéoplasiques, Antinéoplasiques, Chromatographie en phase liquide, Exophiala, Gel de silice, Humains, Lignée cellulaire tumorale, Microbiologie du sol, Milieux de culture, Spectroscopie par résonance magnétique, Structure moléculaire, Tensioactifs.
English descriptors
- KwdEn :
- Antibiotics, Antineoplastic (chemistry), Antibiotics, Antineoplastic (isolation & purification), Antibiotics, Antineoplastic (metabolism), Antibiotics, Antineoplastic (pharmacology), Antineoplastic Agents (chemistry), Antineoplastic Agents (isolation & purification), Antineoplastic Agents (metabolism), Antineoplastic Agents (pharmacology), Cell Line, Tumor, Chromatography, Liquid, Culture Media (chemistry), Exophiala (classification), Exophiala (genetics), Exophiala (isolation & purification), Exophiala (metabolism), Humans, Magnetic Resonance Spectroscopy, Molecular Structure, Plant Oils (metabolism), Silica Gel, Soil Microbiology, Surface-Active Agents (chemistry), Surface-Active Agents (isolation & purification), Surface-Active Agents (metabolism), Surface-Active Agents (pharmacology).
- MESH :
- chemical , chemistry : Antibiotics, Antineoplastic, Antineoplastic Agents, Culture Media, Surface-Active Agents.
- chemical , isolation & purification : Antibiotics, Antineoplastic, Antineoplastic Agents, Surface-Active Agents.
- chemical , metabolism : Antibiotics, Antineoplastic, Antineoplastic Agents, Plant Oils, Surface-Active Agents.
- chemical , pharmacology : Antibiotics, Antineoplastic, Antineoplastic Agents, Surface-Active Agents.
- classification : Exophiala.
- genetics : Exophiala.
- isolation & purification : Exophiala.
- metabolism : Exophiala.
- Cell Line, Tumor, Chromatography, Liquid, Humans, Magnetic Resonance Spectroscopy, Molecular Structure, Silica Gel, Soil Microbiology.
Abstract
A new biosurfactant producer was isolated from palm-oilcontaminated soil and later identified through morphology and DNA sequencing as the yeast-like fungus Exophiala dermatitidis. Biosurfactant production was catalyzed by vegetable oil, supplemented with a basal medium. The culture conditions that provided the biosurfactant with the highest surface activity were found to be 5% palm oil with 0.08% NH4NO3, at a pH of 5.3, with shaking at 200 rpm, and a temperature of 30 degrees C for a 14-day period of incubation. The biosurfactant was purified, in accordance with surfactant properties, by solvent fractionation using silica gel column chromatography. The chemical structure of the strongest surface-active compound was elucidated through the use of NMR and mass spectroscopy, and noted to be monoolein, which then went on to demonstrate antiproliferative activity against cervical cancer (HeLa) and leukemia (U937) cell lines in a dose-dependent manner. Interestingly, no cytotoxicity was observed with normal cells even when high concentrations were used. Cell and DNA morphological changes, in both cancer cell lines, were observed to be cell shrinkage, membrane blebbling, and DNA fragmentation.
PubMed: 21193821
Affiliations:
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Le document en format XML
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<term>Magnetic Resonance Spectroscopy</term>
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<term>Silica Gel</term>
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<term>Antibiotiques antinéoplasiques (pharmacologie)</term>
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<term>Antinéoplasiques (isolement et purification)</term>
<term>Antinéoplasiques (métabolisme)</term>
<term>Antinéoplasiques (pharmacologie)</term>
<term>Chromatographie en phase liquide</term>
<term>Exophiala ()</term>
<term>Exophiala (génétique)</term>
<term>Exophiala (isolement et purification)</term>
<term>Exophiala (métabolisme)</term>
<term>Gel de silice</term>
<term>Huiles végétales (métabolisme)</term>
<term>Humains</term>
<term>Lignée cellulaire tumorale</term>
<term>Microbiologie du sol</term>
<term>Milieux de culture ()</term>
<term>Spectroscopie par résonance magnétique</term>
<term>Structure moléculaire</term>
<term>Tensioactifs ()</term>
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<term>Tensioactifs (métabolisme)</term>
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<term>Surface-Active Agents</term>
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<front><div type="abstract" xml:lang="en">A new biosurfactant producer was isolated from palm-oilcontaminated soil and later identified through morphology and DNA sequencing as the yeast-like fungus Exophiala dermatitidis. Biosurfactant production was catalyzed by vegetable oil, supplemented with a basal medium. The culture conditions that provided the biosurfactant with the highest surface activity were found to be 5% palm oil with 0.08% NH4NO3, at a pH of 5.3, with shaking at 200 rpm, and a temperature of 30 degrees C for a 14-day period of incubation. The biosurfactant was purified, in accordance with surfactant properties, by solvent fractionation using silica gel column chromatography. The chemical structure of the strongest surface-active compound was elucidated through the use of NMR and mass spectroscopy, and noted to be monoolein, which then went on to demonstrate antiproliferative activity against cervical cancer (HeLa) and leukemia (U937) cell lines in a dose-dependent manner. Interestingly, no cytotoxicity was observed with normal cells even when high concentrations were used. Cell and DNA morphological changes, in both cancer cell lines, were observed to be cell shrinkage, membrane blebbling, and DNA fragmentation.</div>
</front>
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<name sortKey="Phonnok, Sirinet" sort="Phonnok, Sirinet" uniqKey="Phonnok S" first="Sirinet" last="Phonnok">Sirinet Phonnok</name>
<name sortKey="Thanomsub, Benjamas Wongsatayanon" sort="Thanomsub, Benjamas Wongsatayanon" uniqKey="Thanomsub B" first="Benjamas Wongsatayanon" last="Thanomsub">Benjamas Wongsatayanon Thanomsub</name>
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