Bioproduction and anticancer activity of biosurfactant produced by the dematiaceous fungus Exophiala dermatitidis SK80.
Identifieur interne : 000D75 ( Main/Curation ); précédent : 000D74; suivant : 000D76Bioproduction and anticancer activity of biosurfactant produced by the dematiaceous fungus Exophiala dermatitidis SK80.
Auteurs : Paramaporn Chiewpattanakul [France] ; Sirinet Phonnok ; Alain Durand ; Emmanuelle Marie ; Benjamas Wongsatayanon ThanomsubSource :
- Journal of microbiology and biotechnology [ 1738-8872 ] ; 2010.
Descripteurs français
- KwdFr :
- Antibiotiques antinéoplasiques (), Antibiotiques antinéoplasiques (isolement et purification), Antibiotiques antinéoplasiques (métabolisme), Antibiotiques antinéoplasiques (pharmacologie), Antinéoplasiques (), Antinéoplasiques (isolement et purification), Antinéoplasiques (métabolisme), Antinéoplasiques (pharmacologie), Chromatographie en phase liquide, Exophiala (), Exophiala (génétique), Exophiala (isolement et purification), Exophiala (métabolisme), Gel de silice, Huiles végétales (métabolisme), Humains, Lignée cellulaire tumorale, Microbiologie du sol, Milieux de culture (), Spectroscopie par résonance magnétique, Structure moléculaire, Tensioactifs (), Tensioactifs (isolement et purification), Tensioactifs (métabolisme), Tensioactifs (pharmacologie).
- MESH :
- génétique : Exophiala.
- isolement et purification : Antibiotiques antinéoplasiques, Antinéoplasiques, Exophiala, Tensioactifs.
- métabolisme : Antibiotiques antinéoplasiques, Antinéoplasiques, Exophiala, Huiles végétales, Tensioactifs.
- pharmacologie : Antibiotiques antinéoplasiques, Antinéoplasiques, Tensioactifs.
- Antibiotiques antinéoplasiques, Antinéoplasiques, Chromatographie en phase liquide, Exophiala, Gel de silice, Humains, Lignée cellulaire tumorale, Microbiologie du sol, Milieux de culture, Spectroscopie par résonance magnétique, Structure moléculaire, Tensioactifs.
English descriptors
- KwdEn :
- Antibiotics, Antineoplastic (chemistry), Antibiotics, Antineoplastic (isolation & purification), Antibiotics, Antineoplastic (metabolism), Antibiotics, Antineoplastic (pharmacology), Antineoplastic Agents (chemistry), Antineoplastic Agents (isolation & purification), Antineoplastic Agents (metabolism), Antineoplastic Agents (pharmacology), Cell Line, Tumor, Chromatography, Liquid, Culture Media (chemistry), Exophiala (classification), Exophiala (genetics), Exophiala (isolation & purification), Exophiala (metabolism), Humans, Magnetic Resonance Spectroscopy, Molecular Structure, Plant Oils (metabolism), Silica Gel, Soil Microbiology, Surface-Active Agents (chemistry), Surface-Active Agents (isolation & purification), Surface-Active Agents (metabolism), Surface-Active Agents (pharmacology).
- MESH :
- chemical , chemistry : Antibiotics, Antineoplastic, Antineoplastic Agents, Culture Media, Surface-Active Agents.
- chemical , isolation & purification : Antibiotics, Antineoplastic, Antineoplastic Agents, Surface-Active Agents.
- chemical , metabolism : Antibiotics, Antineoplastic, Antineoplastic Agents, Plant Oils, Surface-Active Agents.
- chemical , pharmacology : Antibiotics, Antineoplastic, Antineoplastic Agents, Surface-Active Agents.
- classification : Exophiala.
- genetics : Exophiala.
- isolation & purification : Exophiala.
- metabolism : Exophiala.
- Cell Line, Tumor, Chromatography, Liquid, Humans, Magnetic Resonance Spectroscopy, Molecular Structure, Silica Gel, Soil Microbiology.
Abstract
A new biosurfactant producer was isolated from palm-oilcontaminated soil and later identified through morphology and DNA sequencing as the yeast-like fungus Exophiala dermatitidis. Biosurfactant production was catalyzed by vegetable oil, supplemented with a basal medium. The culture conditions that provided the biosurfactant with the highest surface activity were found to be 5% palm oil with 0.08% NH4NO3, at a pH of 5.3, with shaking at 200 rpm, and a temperature of 30 degrees C for a 14-day period of incubation. The biosurfactant was purified, in accordance with surfactant properties, by solvent fractionation using silica gel column chromatography. The chemical structure of the strongest surface-active compound was elucidated through the use of NMR and mass spectroscopy, and noted to be monoolein, which then went on to demonstrate antiproliferative activity against cervical cancer (HeLa) and leukemia (U937) cell lines in a dose-dependent manner. Interestingly, no cytotoxicity was observed with normal cells even when high concentrations were used. Cell and DNA morphological changes, in both cancer cell lines, were observed to be cell shrinkage, membrane blebbling, and DNA fragmentation.
PubMed: 21193821
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000102
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :000102
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000102
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000194
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :000194
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000194
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :000F50
Links to Exploration step
pubmed:21193821Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Bioproduction and anticancer activity of biosurfactant produced by the dematiaceous fungus Exophiala dermatitidis SK80.</title><author><name sortKey="Chiewpattanakul, Paramaporn" sort="Chiewpattanakul, Paramaporn" uniqKey="Chiewpattanakul P" first="Paramaporn" last="Chiewpattanakul">Paramaporn Chiewpattanakul</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Chimie Physique Macromoleculaire CNRS-Nancy University, ENSIC, 1 rue Grandville, BP 20451, F-54001 Nancycedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Chimie Physique Macromoleculaire CNRS-Nancy University, ENSIC, 1 rue Grandville, BP 20451, F-54001 Nancycedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Nancy</settlement></placeName></affiliation></author><author><name sortKey="Phonnok, Sirinet" sort="Phonnok, Sirinet" uniqKey="Phonnok S" first="Sirinet" last="Phonnok">Sirinet Phonnok</name></author><author><name sortKey="Durand, Alain" sort="Durand, Alain" uniqKey="Durand A" first="Alain" last="Durand">Alain Durand</name></author><author><name sortKey="Marie, Emmanuelle" sort="Marie, Emmanuelle" uniqKey="Marie E" first="Emmanuelle" last="Marie">Emmanuelle Marie</name></author><author><name sortKey="Thanomsub, Benjamas Wongsatayanon" sort="Thanomsub, Benjamas Wongsatayanon" uniqKey="Thanomsub B" first="Benjamas Wongsatayanon" last="Thanomsub">Benjamas Wongsatayanon Thanomsub</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:21193821</idno><idno type="pmid">21193821</idno><idno type="wicri:Area/PubMed/Corpus">000102</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000102</idno><idno type="wicri:Area/PubMed/Curation">000102</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000102</idno><idno type="wicri:Area/PubMed/Checkpoint">000102</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000102</idno><idno type="wicri:Area/Ncbi/Merge">000194</idno><idno type="wicri:Area/Ncbi/Curation">000194</idno><idno type="wicri:Area/Ncbi/Checkpoint">000194</idno><idno type="wicri:Area/Main/Merge">000F50</idno><idno type="wicri:Area/Main/Curation">000D75</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Bioproduction and anticancer activity of biosurfactant produced by the dematiaceous fungus Exophiala dermatitidis SK80.</title><author><name sortKey="Chiewpattanakul, Paramaporn" sort="Chiewpattanakul, Paramaporn" uniqKey="Chiewpattanakul P" first="Paramaporn" last="Chiewpattanakul">Paramaporn Chiewpattanakul</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Chimie Physique Macromoleculaire CNRS-Nancy University, ENSIC, 1 rue Grandville, BP 20451, F-54001 Nancycedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Chimie Physique Macromoleculaire CNRS-Nancy University, ENSIC, 1 rue Grandville, BP 20451, F-54001 Nancycedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Nancy</settlement></placeName></affiliation></author><author><name sortKey="Phonnok, Sirinet" sort="Phonnok, Sirinet" uniqKey="Phonnok S" first="Sirinet" last="Phonnok">Sirinet Phonnok</name></author><author><name sortKey="Durand, Alain" sort="Durand, Alain" uniqKey="Durand A" first="Alain" last="Durand">Alain Durand</name></author><author><name sortKey="Marie, Emmanuelle" sort="Marie, Emmanuelle" uniqKey="Marie E" first="Emmanuelle" last="Marie">Emmanuelle Marie</name></author><author><name sortKey="Thanomsub, Benjamas Wongsatayanon" sort="Thanomsub, Benjamas Wongsatayanon" uniqKey="Thanomsub B" first="Benjamas Wongsatayanon" last="Thanomsub">Benjamas Wongsatayanon Thanomsub</name></author></analytic><series><title level="j">Journal of microbiology and biotechnology</title><idno type="eISSN">1738-8872</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibiotics, Antineoplastic (chemistry)</term><term>Antibiotics, Antineoplastic (isolation & purification)</term><term>Antibiotics, Antineoplastic (metabolism)</term><term>Antibiotics, Antineoplastic (pharmacology)</term><term>Antineoplastic Agents (chemistry)</term><term>Antineoplastic Agents (isolation & purification)</term><term>Antineoplastic Agents (metabolism)</term><term>Antineoplastic Agents (pharmacology)</term><term>Cell Line, Tumor</term><term>Chromatography, Liquid</term><term>Culture Media (chemistry)</term><term>Exophiala (classification)</term><term>Exophiala (genetics)</term><term>Exophiala (isolation & purification)</term><term>Exophiala (metabolism)</term><term>Humans</term><term>Magnetic Resonance Spectroscopy</term><term>Molecular Structure</term><term>Plant Oils (metabolism)</term><term>Silica Gel</term><term>Soil Microbiology</term><term>Surface-Active Agents (chemistry)</term><term>Surface-Active Agents (isolation & purification)</term><term>Surface-Active Agents (metabolism)</term><term>Surface-Active Agents (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antibiotiques antinéoplasiques ()</term><term>Antibiotiques antinéoplasiques (isolement et purification)</term><term>Antibiotiques antinéoplasiques (métabolisme)</term><term>Antibiotiques antinéoplasiques (pharmacologie)</term><term>Antinéoplasiques ()</term><term>Antinéoplasiques (isolement et purification)</term><term>Antinéoplasiques (métabolisme)</term><term>Antinéoplasiques (pharmacologie)</term><term>Chromatographie en phase liquide</term><term>Exophiala ()</term><term>Exophiala (génétique)</term><term>Exophiala (isolement et purification)</term><term>Exophiala (métabolisme)</term><term>Gel de silice</term><term>Huiles végétales (métabolisme)</term><term>Humains</term><term>Lignée cellulaire tumorale</term><term>Microbiologie du sol</term><term>Milieux de culture ()</term><term>Spectroscopie par résonance magnétique</term><term>Structure moléculaire</term><term>Tensioactifs ()</term><term>Tensioactifs (isolement et purification)</term><term>Tensioactifs (métabolisme)</term><term>Tensioactifs (pharmacologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antibiotics, Antineoplastic</term><term>Antineoplastic Agents</term><term>Culture Media</term><term>Surface-Active Agents</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibiotics, Antineoplastic</term><term>Antineoplastic Agents</term><term>Surface-Active Agents</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antibiotics, Antineoplastic</term><term>Antineoplastic Agents</term><term>Plant Oils</term><term>Surface-Active Agents</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antibiotics, Antineoplastic</term><term>Antineoplastic Agents</term><term>Surface-Active Agents</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Exophiala</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Exophiala</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Exophiala</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Exophiala</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antibiotiques antinéoplasiques</term><term>Antinéoplasiques</term><term>Exophiala</term><term>Tensioactifs</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Exophiala</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antibiotiques antinéoplasiques</term><term>Antinéoplasiques</term><term>Exophiala</term><term>Huiles végétales</term><term>Tensioactifs</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antibiotiques antinéoplasiques</term><term>Antinéoplasiques</term><term>Tensioactifs</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line, Tumor</term><term>Chromatography, Liquid</term><term>Humans</term><term>Magnetic Resonance Spectroscopy</term><term>Molecular Structure</term><term>Silica Gel</term><term>Soil Microbiology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Antibiotiques antinéoplasiques</term><term>Antinéoplasiques</term><term>Chromatographie en phase liquide</term><term>Exophiala</term><term>Gel de silice</term><term>Humains</term><term>Lignée cellulaire tumorale</term><term>Microbiologie du sol</term><term>Milieux de culture</term><term>Spectroscopie par résonance magnétique</term><term>Structure moléculaire</term><term>Tensioactifs</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A new biosurfactant producer was isolated from palm-oilcontaminated soil and later identified through morphology and DNA sequencing as the yeast-like fungus Exophiala dermatitidis. Biosurfactant production was catalyzed by vegetable oil, supplemented with a basal medium. The culture conditions that provided the biosurfactant with the highest surface activity were found to be 5% palm oil with 0.08% NH4NO3, at a pH of 5.3, with shaking at 200 rpm, and a temperature of 30 degrees C for a 14-day period of incubation. The biosurfactant was purified, in accordance with surfactant properties, by solvent fractionation using silica gel column chromatography. The chemical structure of the strongest surface-active compound was elucidated through the use of NMR and mass spectroscopy, and noted to be monoolein, which then went on to demonstrate antiproliferative activity against cervical cancer (HeLa) and leukemia (U937) cell lines in a dose-dependent manner. Interestingly, no cytotoxicity was observed with normal cells even when high concentrations were used. Cell and DNA morphological changes, in both cancer cell lines, were observed to be cell shrinkage, membrane blebbling, and DNA fragmentation.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Lorraine/explor/LrgpV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D75 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 000D75 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Lorraine
|area= LrgpV1
|flux= Main
|étape= Curation
|type= RBID
|clé= pubmed:21193821
|texte= Bioproduction and anticancer activity of biosurfactant produced by the dematiaceous fungus Exophiala dermatitidis SK80.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:21193821" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \
| NlmPubMed2Wicri -a LrgpV1
| This area was generated with Dilib version V0.6.32. |  |