LrgpV1, Main, Curation, bibRecord, 000280

CTAB turbidimetric method for assaying hyaluronic acid in complex environments and under cross-linked form

Identifieur interne : 000280 ( Main/Curation ); précédent : 000279; suivant : 000281

CTAB turbidimetric method for assaying hyaluronic acid in complex environments and under cross-linked form

Auteurs : Nadia Oueslati [France] ; Pierrick Leblanc [France] ; Christelle Harscoat-Schiavo [France] ; Emmanuel Rondags [France] ; Stéphane Meunier ; Romain Kapel [France] ; Ivan Marc [France]

Source :

Carbohydrate Polymers [ 0144-8617 ] ; 2014-11.

RBID : Hal:hal-01274049

Abstract

The cetyltrimethylammonium bromide turbidimetric method (CTM) has been developed to quantify the hyaluronic acid (HA) in complex media to overcome the lack of selectivity and specificity of the standard carbazole method. The objective of this work is to assess the potential application of CTM to determine HA concentration. Factors such as duration of incubation, linearity range, HA size and form (natural linear HA or cross linked HA), pH and ionic environment impact were investigated. The incubation time was set to 10 min and the calibration curve was linear up to 0.6 g L-1. The quantitative method was relevant whatever the HA size and form, and also for a wide range of conditions. The robustness of the CTM added to its high specificity and simplicity demonstrated that the CTM is a valuable method that would be an interesting substitute to the carbazole assay for HA quantification.

Url:

https://hal.univ-lorraine.fr/hal-01274049

DOI: 10.1016/j.carbpol.2014.05.039

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Le document en format XML

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<author><name sortKey="Rondags, Emmanuel" sort="Rondags, Emmanuel" uniqKey="Rondags E" first="Emmanuel" last="Rondags">Emmanuel Rondags</name>
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<settlement type="city">Metz</settlement>
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<author><name sortKey="Meunier, Stephane" sort="Meunier, Stephane" uniqKey="Meunier S" first="Stéphane" last="Meunier">Stéphane Meunier</name>
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<author><name sortKey="Kapel, Romain" sort="Kapel, Romain" uniqKey="Kapel R" first="Romain" last="Kapel">Romain Kapel</name>
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<author><name sortKey="Marc, Ivan" sort="Marc, Ivan" uniqKey="Marc I" first="Ivan" last="Marc">Ivan Marc</name>
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<orgName>Université de Lorraine</orgName>
<orgName type="acronym">UL</orgName>
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<country>France</country>
<placeName><settlement type="city">Nancy</settlement>
<settlement type="city">Metz</settlement>
<region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
</placeName>
<orgName type="university">Université de Lorraine</orgName>
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</author>
</analytic>
<idno type="DOI">10.1016/j.carbpol.2014.05.039</idno>
<series><title level="j">Carbohydrate Polymers</title>
<idno type="ISSN">0144-8617</idno>
<imprint><date type="datePub">2014-11</date>
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<front><div type="abstract" xml:lang="en">The cetyltrimethylammonium bromide turbidimetric method (CTM) has been developed to quantify the hyaluronic acid (HA) in complex media to overcome the lack of selectivity and specificity of the standard carbazole method. The objective of this work is to assess the potential application of CTM to determine HA concentration. Factors such as duration of incubation, linearity range, HA size and form (natural linear HA or cross linked HA), pH and ionic environment impact were investigated. The incubation time was set to 10 min and the calibration curve was linear up to 0.6 g L-1. The quantitative method was relevant whatever the HA size and form, and also for a wide range of conditions. The robustness of the CTM added to its high specificity and simplicity demonstrated that the CTM is a valuable method that would be an interesting substitute to the carbazole assay for HA quantification.</div>
</front>
</TEI>
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CTAB turbidimetric method for assaying hyaluronic acid in complex environments and under cross-linked form

CTAB turbidimetric method for assaying hyaluronic acid in complex environments and under cross-linked form

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