Quantum Dot-folic acid conjugates as potential photosensitizers in photodynamic therapy of cancer
Identifieur interne : 000958 ( Hal/Corpus ); précédent : 000957; suivant : 000959Quantum Dot-folic acid conjugates as potential photosensitizers in photodynamic therapy of cancer
Auteurs : Vincent Morosini ; Thierry Bastogne ; Céline Frochot ; Raphaël Schneider ; Aurélie François ; François Guillemin ; Muriel Barberi-HeyobSource :
- Photochemical & Photobiological Sciences Photochemical and Photobiological Sciences [ 1474-905X ] ; 2011-05.
Abstract
This study examined the in vitro potential of bioconjugated quantum dots (QDs) as photosensitizers for photodynamic therapy (PDT). According to our previous approaches using photosensitizers, folic acid appears to be an optimal targeting ligand for selective delivery of attached therapeutic agents to cancer tissues. We synthesized hydrophilic near infrared emitting CdTe(S)-type QDs conjugated with folic acid using different spacers. Photodynamic efficiency of QDs conjugated or not with folic acid was evaluated on KB cells, acting as a positive control due to their overexpression of FR-α, and HT-29 cells lacking FR-α, as negative control. A design of experiments was suggested as a rational solution to evaluate the impacts of each experimental factor (QD type and concentration, light fluence and excitation wavelength, time of contact before irradiation and cell phenotype). We demonstrated that, for concentrations lower than 10 nM, QDs displayed practically no cytotoxic effect without light exposure for both cell lines. Whereas QDs at 2.1 nM displayed a weak photodynamic activity, a concentration of 8 nM significantly enhanced the photodynamic efficiency characterized by a light dose-dependent response. A statistically significant difference in photodynamic efficiency between KB and HT-29 cells was evidenced in the case of folic acid-conjugated QDs. Optimal conditions led to an enhanced photocytotoxicity response, allowing us to validate the ability of QDs to generate a photodynamic effect and of folic acid-conjugated QDs for targeted PDT.
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DOI: 10.1039/C0PP00380H
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Hal:hal-00580535Le document en format XML
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Nancy 1</orgName><orgName type="acronym">UHP</orgName><date type="end">2011-12-31</date><desc><address><addrLine>24-30 rue Lionnois, BP 60120, 54 003 NANCY cedex, France</addrLine><country key="FR"></country></address></desc></org></tutelle><tutelle active="#struct-300293" type="direct"><org type="institution" xml:id="struct-300293" status="OLD"><orgName>Institut National Polytechnique de Lorraine</orgName><orgName type="acronym">INPL</orgName><date type="end">2011-12-31</date><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="UMR7039" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno><idno type="IdRef">02636817X</idno><orgName>Centre National de la Recherche Scientifique</orgName><orgName type="acronym">CNRS</orgName><date type="start">1939-10-19</date><desc><address><country key="FR"></country></address><ref type="url">http://www.cnrs.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Guillemin, Francois" sort="Guillemin, Francois" uniqKey="Guillemin F" first="François" last="Guillemin">François Guillemin</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-1001" status="OLD"><orgName>Centre de Recherche en Automatique de Nancy</orgName><orgName type="acronym">CRAN</orgName><date type="end">2011-12-31</date><desc><address><addrLine>Université Henri Poincaré, 2 avenue de la forêt de Haye, 54516 Vandoeuvre les Nancy</addrLine><country key="FR"></country></address><ref type="url">http://www.cran.uhp-nancy.fr</ref></desc><listRelation><relation active="#struct-300291" type="direct"></relation><relation active="#struct-300293" type="direct"></relation><relation name="UMR7039" active="#struct-441569" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300291" type="direct"><org type="institution" xml:id="struct-300291" status="OLD"><orgName>Université Henri Poincaré - Nancy 1</orgName><orgName type="acronym">UHP</orgName><date type="end">2011-12-31</date><desc><address><addrLine>24-30 rue Lionnois, BP 60120, 54 003 NANCY cedex, France</addrLine><country key="FR"></country></address></desc></org></tutelle><tutelle active="#struct-300293" type="direct"><org type="institution" xml:id="struct-300293" status="OLD"><orgName>Institut National Polytechnique de Lorraine</orgName><orgName type="acronym">INPL</orgName><date type="end">2011-12-31</date><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="UMR7039" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno><idno type="IdRef">02636817X</idno><orgName>Centre National de la Recherche Scientifique</orgName><orgName type="acronym">CNRS</orgName><date type="start">1939-10-19</date><desc><address><country key="FR"></country></address><ref type="url">http://www.cnrs.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Barberi Heyob, Muriel" sort="Barberi Heyob, Muriel" uniqKey="Barberi Heyob M" first="Muriel" last="Barberi-Heyob">Muriel Barberi-Heyob</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-1001" status="OLD"><orgName>Centre de Recherche en Automatique de Nancy</orgName><orgName type="acronym">CRAN</orgName><date type="end">2011-12-31</date><desc><address><addrLine>Université Henri Poincaré, 2 avenue de la forêt de Haye, 54516 Vandoeuvre les Nancy</addrLine><country key="FR"></country></address><ref type="url">http://www.cran.uhp-nancy.fr</ref></desc><listRelation><relation active="#struct-300291" type="direct"></relation><relation active="#struct-300293" type="direct"></relation><relation name="UMR7039" active="#struct-441569" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300291" type="direct"><org type="institution" xml:id="struct-300291" status="OLD"><orgName>Université Henri Poincaré - Nancy 1</orgName><orgName type="acronym">UHP</orgName><date type="end">2011-12-31</date><desc><address><addrLine>24-30 rue Lionnois, BP 60120, 54 003 NANCY cedex, France</addrLine><country key="FR"></country></address></desc></org></tutelle><tutelle active="#struct-300293" type="direct"><org type="institution" xml:id="struct-300293" status="OLD"><orgName>Institut National Polytechnique de Lorraine</orgName><orgName type="acronym">INPL</orgName><date type="end">2011-12-31</date><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="UMR7039" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno><idno type="IdRef">02636817X</idno><orgName>Centre National de la Recherche Scientifique</orgName><orgName type="acronym">CNRS</orgName><date type="start">1939-10-19</date><desc><address><country key="FR"></country></address><ref type="url">http://www.cnrs.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation></affiliation></author></analytic><idno type="DOI">10.1039/C0PP00380H</idno><series><title level="j">Photochemical & Photobiological Sciences Photochemical and Photobiological Sciences</title><idno type="ISSN">1474-905X</idno><imprint><date type="datePub">2011-05</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study examined the in vitro potential of bioconjugated quantum dots (QDs) as photosensitizers for photodynamic therapy (PDT). According to our previous approaches using photosensitizers, folic acid appears to be an optimal targeting ligand for selective delivery of attached therapeutic agents to cancer tissues. We synthesized hydrophilic near infrared emitting CdTe(S)-type QDs conjugated with folic acid using different spacers. Photodynamic efficiency of QDs conjugated or not with folic acid was evaluated on KB cells, acting as a positive control due to their overexpression of FR-α, and HT-29 cells lacking FR-α, as negative control. A design of experiments was suggested as a rational solution to evaluate the impacts of each experimental factor (QD type and concentration, light fluence and excitation wavelength, time of contact before irradiation and cell phenotype). We demonstrated that, for concentrations lower than 10 nM, QDs displayed practically no cytotoxic effect without light exposure for both cell lines. Whereas QDs at 2.1 nM displayed a weak photodynamic activity, a concentration of 8 nM significantly enhanced the photodynamic efficiency characterized by a light dose-dependent response. A statistically significant difference in photodynamic efficiency between KB and HT-29 cells was evidenced in the case of folic acid-conjugated QDs. Optimal conditions led to an enhanced photocytotoxicity response, allowing us to validate the ability of QDs to generate a photodynamic effect and of folic acid-conjugated QDs for targeted PDT.</div></front></TEI><hal api="V3"><titleStmt><title xml:lang="en">Quantum Dot-folic acid conjugates as potential photosensitizers in photodynamic therapy of cancer</title><author role="aut"><persName><forename type="first">Vincent</forename><surname>Morosini</surname></persName><email></email><idno type="halauthor">150169</idno><affiliation ref="#struct-1001"></affiliation></author><author role="aut"><persName><forename type="first">Thierry</forename><surname>Bastogne</surname></persName><email></email><idno type="halauthor">140180</idno><affiliation ref="#struct-1001"></affiliation></author><author role="aut"><persName><forename type="first">Céline</forename><surname>Frochot</surname></persName><email></email><idno type="halauthor">133064</idno><affiliation ref="#struct-73720"></affiliation><affiliation ref="#struct-211875"></affiliation></author><author role="aut"><persName><forename type="first">Raphaël</forename><surname>Schneider</surname></persName><email></email><idno type="halauthor">133063</idno><affiliation ref="#struct-41464"></affiliation></author><author role="aut"><persName><forename type="first">Aurélie</forename><surname>François</surname></persName><email></email><idno type="halauthor">151315</idno><affiliation ref="#struct-1001"></affiliation><affiliation ref="#struct-101696"></affiliation></author><author role="aut"><persName><forename type="first">François</forename><surname>Guillemin</surname></persName><email></email><idno type="idhal">francois-guillemin</idno><idno type="halauthor">89500</idno><affiliation ref="#struct-1001"></affiliation><affiliation ref="#struct-101696"></affiliation></author><author role="aut"><persName><forename type="first">Muriel</forename><surname>Barberi-Heyob</surname></persName><email></email><idno type="halauthor">89501</idno><affiliation ref="#struct-1001"></affiliation><affiliation ref="#struct-73720"></affiliation><affiliation ref="#struct-101696"></affiliation></author><editor role="depositor"><persName><forename>Didier</forename><surname>Wolf</surname></persName><email>Didier.Wolf@univ-lorraine.fr</email></editor></titleStmt><editionStmt><edition n="v1" type="current"><date type="whenSubmitted">2011-03-28 13:51:09</date><date type="whenModified">2016-05-17 10:14:23</date><date type="whenReleased">2011-03-28 13:51:09</date><date type="whenProduced">2011-05</date></edition><respStmt><resp>contributor</resp><name key="114907"><persName><forename>Didier</forename><surname>Wolf</surname></persName><email>Didier.Wolf@univ-lorraine.fr</email></name></respStmt></editionStmt><publicationStmt><distributor>CCSD</distributor><idno type="halId">hal-00580535</idno><idno type="halUri">https://hal.archives-ouvertes.fr/hal-00580535</idno><idno type="halBibtex">morosini:hal-00580535</idno><idno type="halRefHtml">Photochemical & Photobiological Sciences Photochemical and Photobiological Sciences, Royal Society of Chemistry, 2011, 10 (5), pp.842-851. <10.1039/C0PP00380H></idno><idno type="halRef">Photochemical & Photobiological Sciences Photochemical and Photobiological Sciences, Royal Society of Chemistry, 2011, 10 (5), pp.842-851. <10.1039/C0PP00380H></idno></publicationStmt><seriesStmt><idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno><idno type="stamp" n="CRAN">Centre de Recherche en Automatique de Nancy</idno><idno type="stamp" n="LRGP-UL">LRGP</idno><idno type="stamp" n="INPL">Institut National Polytechnique de Lorraine</idno><idno type="stamp" n="UNIV-LORRAINE">Université de Lorraine</idno></seriesStmt><notesStmt><note type="audience" n="2">International</note><note type="popular" n="0">No</note><note type="peer" n="1">Yes</note></notesStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Quantum Dot-folic acid conjugates as potential photosensitizers in photodynamic therapy of cancer</title><author role="aut"><persName><forename type="first">Vincent</forename><surname>Morosini</surname></persName><idno type="halAuthorId">150169</idno><affiliation ref="#struct-1001"></affiliation></author><author role="aut"><persName><forename type="first">Thierry</forename><surname>Bastogne</surname></persName><idno type="halAuthorId">140180</idno><affiliation ref="#struct-1001"></affiliation></author><author role="aut"><persName><forename type="first">Céline</forename><surname>Frochot</surname></persName><idno type="halAuthorId">133064</idno><affiliation ref="#struct-73720"></affiliation><affiliation ref="#struct-211875"></affiliation></author><author role="aut"><persName><forename type="first">Raphaël</forename><surname>Schneider</surname></persName><idno type="halAuthorId">133063</idno><affiliation ref="#struct-41464"></affiliation></author><author role="aut"><persName><forename type="first">Aurélie</forename><surname>François</surname></persName><idno type="halAuthorId">151315</idno><affiliation ref="#struct-1001"></affiliation><affiliation ref="#struct-101696"></affiliation></author><author role="aut"><persName><forename type="first">François</forename><surname>Guillemin</surname></persName><idno type="idHal">francois-guillemin</idno><idno type="halAuthorId">89500</idno><affiliation ref="#struct-1001"></affiliation><affiliation ref="#struct-101696"></affiliation></author><author role="aut"><persName><forename type="first">Muriel</forename><surname>Barberi-Heyob</surname></persName><idno type="halAuthorId">89501</idno><affiliation ref="#struct-1001"></affiliation><affiliation ref="#struct-73720"></affiliation><affiliation ref="#struct-101696"></affiliation></author></analytic><monogr><idno type="halJournalId" status="VALID">17941</idno><idno type="issn">1474-905X</idno><idno type="eissn">1474-9092</idno><title level="j">Photochemical & Photobiological Sciences Photochemical and Photobiological Sciences</title><imprint><publisher>Royal Society of Chemistry</publisher><biblScope unit="volume">10</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="pp">842-851</biblScope><date type="datePub">2011-05</date></imprint></monogr><idno type="doi">10.1039/C0PP00380H</idno></biblStruct></sourceDesc><profileDesc><langUsage><language ident="en">English</language></langUsage><textClass><classCode scheme="halDomain" n="sdv.ib">Life Sciences [q-bio]/Bioengineering</classCode><classCode scheme="halTypology" n="ART">Journal articles</classCode></textClass><abstract xml:lang="en">This study examined the in vitro potential of bioconjugated quantum dots (QDs) as photosensitizers for photodynamic therapy (PDT). According to our previous approaches using photosensitizers, folic acid appears to be an optimal targeting ligand for selective delivery of attached therapeutic agents to cancer tissues. We synthesized hydrophilic near infrared emitting CdTe(S)-type QDs conjugated with folic acid using different spacers. Photodynamic efficiency of QDs conjugated or not with folic acid was evaluated on KB cells, acting as a positive control due to their overexpression of FR-α, and HT-29 cells lacking FR-α, as negative control. A design of experiments was suggested as a rational solution to evaluate the impacts of each experimental factor (QD type and concentration, light fluence and excitation wavelength, time of contact before irradiation and cell phenotype). We demonstrated that, for concentrations lower than 10 nM, QDs displayed practically no cytotoxic effect without light exposure for both cell lines. Whereas QDs at 2.1 nM displayed a weak photodynamic activity, a concentration of 8 nM significantly enhanced the photodynamic efficiency characterized by a light dose-dependent response. A statistically significant difference in photodynamic efficiency between KB and HT-29 cells was evidenced in the case of folic acid-conjugated QDs. Optimal conditions led to an enhanced photocytotoxicity response, allowing us to validate the ability of QDs to generate a photodynamic effect and of folic acid-conjugated QDs for targeted PDT.</abstract></profileDesc></hal></record>Pour manipuler ce document sous Unix (Dilib)
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