CobaltMaghrebV1, PascalFrancis, Corpus, bibRecord, 000081

Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins

Identifieur interne : 000081 ( PascalFrancis/Corpus ); précédent : 000080; suivant : 000082

Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins

Auteurs : Roy M. Pemberton ; Frankie J. Rawson ; JINSHENG XU ; Robin Pittson ; Guido A. Drago ; John Griffiths ; Simon K. Jackson ; John P. Hart

Source :

Mikrochimica acta : (1966. Print) [ 0026-3672 ] ; 2010.

RBID : Pascal:10-0471659

Descripteurs français

Pascal (Inist)
- Application, Biodétecteur, Environnement, Toxine, Carbone, Cobalt, Enzyme, Solution tampon, Voltammétrie cyclique, Densité élevée, Régime permanent, Ampérométrie, Diffusion, Microélectrode, Eau, Encre, Phtalocyanine, Glucose oxidase, Lactate, Hexacyanoferrate II, Phosphate, Utilisation, Platine, Milieu culture, Sensibilité, Dynamique, Temps, Libération, Benzène(1,3-dinitro), Réduction, Production, Culture cellulaire, Toxicité, Monitorage.

English descriptors

KwdEn :
- Amperometry, Application, Biosensor, Buffer solution, Carbon, Cell culture, Cobalt, Culture medium, Cyclic voltammetry, Diffusion, Dynamics, Environment, Enzyme, Glucose oxidase, Hexacyanoferrates II, High density, Ink, Lactates, Microelectrode, Monitoring, Phosphates, Phthalocyanine, Platinum, Production, Reduction, Release, Sensitivity, Steady state, Time, Toxicity, Toxin, Use, Water.

Abstract

Microband biosensors were fabricated from a screen-printed water-based carbon ink containing cobalt phthalocyanine redox mediator and glucose oxidase or lactate oxidase enzyme. The microbiosensors were characterised for their ability to monitor ferrocyanide and H₂O₂ in phosphate buffer solution: sigmoidal cyclic voltammograms, high current density values and steady-state amperometric responses confirmed the existence of radial-diffusion-limiting microelectrode behaviour. The lactate microband biosensors were then used, in conjunction with a screen-printed Ag/AgCl reference and platinum counter electrode, to monitor lactate levels in culture medium, with a linear range of 0.5-5 mM, sensitivity of 20 nA.mM^-1, and dynamic range up to >9 mM. The lactate microband biosensors could operate continuously in culture medium over extended times (up to 24 h) at 37°C. These biosensors were then applied to detect changes in lactate release from cultured cells in response to toxic challenge: m-dinitrobenzene (500 μM) caused a reduction in lactate production by high-passage number HepG2 single cells; D-galactosamine (20 mM) induced release of lactate by HepG2 spheroid cultures. This novel use of microband biosensors in cell culture has the potential for further application in toxicity monitoring, in both environmental and pharmaceutical areas.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A05				`@2 170`
A06				`@2 3-4`
A08	`01`	`1`	`ENG`	`@1 Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins`
A09	`01`	`1`	`ENG`	`@1 Biosensors for Food and the Environment`
A11	`01`	`1`		`@1 PEMBERTON (Roy M.)`
A11	`02`	`1`		`@1 RAWSON (Frankie J.)`
A11	`03`	`1`		`@1 JINSHENG XU`
A11	`04`	`1`		`@1 PITTSON (Robin)`
A11	`05`	`1`		`@1 DRAGO (Guido A.)`
A11	`06`	`1`		`@1 GRIFFITHS (John)`
A11	`07`	`1`		`@1 JACKSON (Simon K.)`
A11	`08`	`1`		`@1 HART (John P.)`
A12	`01`	`1`		`@1 AMINE (Aziz) @9 ed.`
A12	`02`	`1`		`@1 BRETT (Chris M. A.) @9 ed.`
A12	`03`	`1`		`@1 PALLESCHI (Giuseppe) @9 ed.`
A14	`01`			`@1 Centre for Analytical, Materials and Sensors Science, Faculty of Health and Life Sciences, University of the West of England @2 Bristol BS16 1QY @3 GBR @Z 1 aut. @Z 2 aut. @Z 8 aut.`
A14	`02`			`@1 Centre for Research in Biomedicine, Faculty of Health and Life Sciences, University of the West of England @2 Bristol BS16 1QY @3 GBR @Z 3 aut. @Z 7 aut.`
A14	`03`			`@1 Gwent Electronic Materials Ltd., Monmouth House, Mamhilad Business Park @2 Pontypool NP4 OHZ @3 GBR @Z 4 aut.`
A14	`04`			`@1 Applied Enzyme Technology Ltd., Monmouth House, Mamhilad Business Park @2 Pontypool, Gwent NP4 OHZ @3 GBR @Z 5 aut.`
A14	`05`			`@1 Uniscan Instruments Ltd. @2 Buxton @3 GBR @Z 6 aut.`
A15	`01`			`@1 Faculté des Sciences et Techniques, Université Hassan II-Mohammedia, B.P. 146 @2 Mohammedia @3 MAR @Z 1 aut.`
A15	`02`			`@1 Universidade de Coimbra @2 3004-535 Coimbra @3 PRT @Z 2 aut.`
A15	`03`			`@1 Università di Roma Tor Vergata @2 00133 Roma @3 ITA @Z 3 aut.`
A20				`@1 321-330`
A21				`@1 2010`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 3977 @5 354000192698520140`
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C01	`01`		`ENG`	@0 Microband biosensors were fabricated from a screen-printed water-based carbon ink containing cobalt phthalocyanine redox mediator and glucose oxidase or lactate oxidase enzyme. The microbiosensors were characterised for their ability to monitor ferrocyanide and H₂O₂ in phosphate buffer solution: sigmoidal cyclic voltammograms, high current density values and steady-state amperometric responses confirmed the existence of radial-diffusion-limiting microelectrode behaviour. The lactate microband biosensors were then used, in conjunction with a screen-printed Ag/AgCl reference and platinum counter electrode, to monitor lactate levels in culture medium, with a linear range of 0.5-5 mM, sensitivity of 20 nA.mM^-1, and dynamic range up to >9 mM. The lactate microband biosensors could operate continuously in culture medium over extended times (up to 24 h) at 37°C. These biosensors were then applied to detect changes in lactate release from cultured cells in response to toxic challenge: m-dinitrobenzene (500 μM) caused a reduction in lactate production by high-passage number HepG2 single cells; D-galactosamine (20 mM) induced release of lactate by HepG2 spheroid cultures. This novel use of microband biosensors in cell culture has the potential for further application in toxicity monitoring, in both environmental and pharmaceutical areas.
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C03	`01`	`X`	`ENG`	`@0 Application @5 01`
C03	`01`	`X`	`SPA`	`@0 Aplicación @5 01`
C03	`02`	`X`	`FRE`	`@0 Biodétecteur @5 02`
C03	`02`	`X`	`ENG`	`@0 Biosensor @5 02`
C03	`02`	`X`	`SPA`	`@0 Biodetector @5 02`
C03	`03`	`X`	`FRE`	`@0 Environnement @5 03`
C03	`03`	`X`	`ENG`	`@0 Environment @5 03`
C03	`03`	`X`	`SPA`	`@0 Medio ambiente @5 03`
C03	`04`	`X`	`FRE`	`@0 Toxine @5 04`
C03	`04`	`X`	`ENG`	`@0 Toxin @5 04`
C03	`04`	`X`	`SPA`	`@0 Toxina @5 04`
C03	`05`	`X`	`FRE`	`@0 Carbone @2 NC @5 05`
C03	`05`	`X`	`ENG`	`@0 Carbon @2 NC @5 05`
C03	`05`	`X`	`SPA`	`@0 Carbono @2 NC @5 05`
C03	`06`	`X`	`FRE`	`@0 Cobalt @2 NC @5 06`
C03	`06`	`X`	`ENG`	`@0 Cobalt @2 NC @5 06`
C03	`06`	`X`	`SPA`	`@0 Cobalto @2 NC @5 06`
C03	`07`	`X`	`FRE`	`@0 Enzyme @2 FE @5 07`
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C03	`08`	`X`	`FRE`	`@0 Solution tampon @5 08`
C03	`08`	`X`	`ENG`	`@0 Buffer solution @5 08`
C03	`08`	`X`	`SPA`	`@0 Solución de amortiguación @5 08`
C03	`09`	`X`	`FRE`	`@0 Voltammétrie cyclique @5 09`
C03	`09`	`X`	`ENG`	`@0 Cyclic voltammetry @5 09`
C03	`09`	`X`	`SPA`	`@0 Voltametría cíclica @5 09`
C03	`10`	`X`	`FRE`	`@0 Densité élevée @5 10`
C03	`10`	`X`	`ENG`	`@0 High density @5 10`
C03	`10`	`X`	`SPA`	`@0 Densidad elevada @5 10`
C03	`11`	`X`	`FRE`	`@0 Régime permanent @5 11`
C03	`11`	`X`	`ENG`	`@0 Steady state @5 11`
C03	`11`	`X`	`SPA`	`@0 Régimen permanente @5 11`
C03	`12`	`X`	`FRE`	`@0 Ampérométrie @5 12`
C03	`12`	`X`	`ENG`	`@0 Amperometry @5 12`
C03	`12`	`X`	`SPA`	`@0 Amperometría @5 12`
C03	`13`	`X`	`FRE`	`@0 Diffusion @5 13`
C03	`13`	`X`	`ENG`	`@0 Diffusion @5 13`
C03	`13`	`X`	`SPA`	`@0 Difusión @5 13`
C03	`14`	`X`	`FRE`	`@0 Microélectrode @5 14`
C03	`14`	`X`	`ENG`	`@0 Microelectrode @5 14`
C03	`14`	`X`	`SPA`	`@0 Microeléctrodo @5 14`
C03	`15`	`X`	`FRE`	`@0 Eau @5 15`
C03	`15`	`X`	`ENG`	`@0 Water @5 15`
C03	`15`	`X`	`SPA`	`@0 Agua @5 15`
C03	`16`	`X`	`FRE`	`@0 Encre @5 16`
C03	`16`	`X`	`ENG`	`@0 Ink @5 16`
C03	`16`	`X`	`SPA`	`@0 Tinta @5 16`
C03	`17`	`X`	`FRE`	`@0 Phtalocyanine @2 NK @5 17`
C03	`17`	`X`	`ENG`	`@0 Phthalocyanine @2 NK @5 17`
C03	`17`	`X`	`SPA`	`@0 Ftalocianina @2 NK @5 17`
C03	`18`	`X`	`FRE`	`@0 Glucose oxidase @2 FE @5 18`
C03	`18`	`X`	`ENG`	`@0 Glucose oxidase @2 FE @5 18`
C03	`18`	`X`	`SPA`	`@0 Glucose oxidase @2 FE @5 18`
C03	`19`	`X`	`FRE`	`@0 Lactate @5 19`
C03	`19`	`X`	`ENG`	`@0 Lactates @5 19`
C03	`19`	`X`	`SPA`	`@0 Lactato @5 19`
C03	`20`	`X`	`FRE`	`@0 Hexacyanoferrate II @2 NA @5 20`
C03	`20`	`X`	`ENG`	`@0 Hexacyanoferrates II @2 NA @5 20`
C03	`20`	`X`	`SPA`	`@0 Hexacianoferrato II @2 NA @5 20`
C03	`21`	`X`	`FRE`	`@0 Phosphate @2 NA @5 21`
C03	`21`	`X`	`ENG`	`@0 Phosphates @2 NA @5 21`
C03	`21`	`X`	`SPA`	`@0 Fosfato @2 NA @5 21`
C03	`22`	`X`	`FRE`	`@0 Utilisation @5 22`
C03	`22`	`X`	`ENG`	`@0 Use @5 22`
C03	`22`	`X`	`SPA`	`@0 Uso @5 22`
C03	`23`	`X`	`FRE`	`@0 Platine @2 NC @5 23`
C03	`23`	`X`	`ENG`	`@0 Platinum @2 NC @5 23`
C03	`23`	`X`	`SPA`	`@0 Platino @2 NC @5 23`
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C03	`26`	`X`	`FRE`	`@0 Dynamique @5 26`
C03	`26`	`X`	`ENG`	`@0 Dynamics @5 26`
C03	`26`	`X`	`SPA`	`@0 Dinámica @5 26`
C03	`27`	`X`	`FRE`	`@0 Temps @5 27`
C03	`27`	`X`	`ENG`	`@0 Time @5 27`
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C03	`28`	`X`	`FRE`	`@0 Libération @5 28`
C03	`28`	`X`	`ENG`	`@0 Release @5 28`
C03	`28`	`X`	`SPA`	`@0 Liberación @5 28`
C03	`29`	`X`	`FRE`	`@0 Benzène(1,3-dinitro) @2 NK @2 FX @5 29`
C03	`30`	`X`	`FRE`	`@0 Réduction @5 30`
C03	`30`	`X`	`ENG`	`@0 Reduction @5 30`
C03	`30`	`X`	`SPA`	`@0 Reducción @5 30`
C03	`31`	`X`	`FRE`	`@0 Production @5 41`
C03	`31`	`X`	`ENG`	`@0 Production @5 41`
C03	`31`	`X`	`SPA`	`@0 Producción @5 41`
C03	`32`	`X`	`FRE`	`@0 Culture cellulaire @5 42`
C03	`32`	`X`	`ENG`	`@0 Cell culture @5 42`
C03	`32`	`X`	`SPA`	`@0 Cultivo celular @5 42`
C03	`33`	`X`	`FRE`	`@0 Toxicité @5 43`
C03	`33`	`X`	`ENG`	`@0 Toxicity @5 43`
C03	`33`	`X`	`SPA`	`@0 Toxicidad @5 43`
C03	`34`	`X`	`FRE`	`@0 Monitorage @5 44`
C03	`34`	`X`	`ENG`	`@0 Monitoring @5 44`
C03	`34`	`X`	`SPA`	`@0 Monitoreo @5 44`
C07	`01`	`X`	`FRE`	`@0 Oxidoreductases @2 FE`
C07	`01`	`X`	`ENG`	`@0 Oxidoreductases @2 FE`
C07	`01`	`X`	`SPA`	`@0 Oxidoreductases @2 FE`
N21				`@1 305`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

A30	`01`	`1`	`ENG`	`@1 International Workshop on Biosensors for Food Safety and Environmental Monitoring @2 4 @3 Tangier MAR @4 2009-10-01`

Format Inist (serveur)

NO :	PASCAL 10-0471659 INIST
ET :	Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins
AU :	PEMBERTON (Roy M.); RAWSON (Frankie J.); JINSHENG XU; PITTSON (Robin); DRAGO (Guido A.); GRIFFITHS (John); JACKSON (Simon K.); HART (John P.); AMINE (Aziz); BRETT (Chris M. A.); PALLESCHI (Giuseppe)
AF :	Centre for Analytical, Materials and Sensors Science, Faculty of Health and Life Sciences, University of the West of England/Bristol BS16 1QY/Royaume-Uni (1 aut., 2 aut., 8 aut.); Centre for Research in Biomedicine, Faculty of Health and Life Sciences, University of the West of England/Bristol BS16 1QY/Royaume-Uni (3 aut., 7 aut.); Gwent Electronic Materials Ltd., Monmouth House, Mamhilad Business Park/Pontypool NP4 OHZ/Royaume-Uni (4 aut.); Applied Enzyme Technology Ltd., Monmouth House, Mamhilad Business Park/Pontypool, Gwent NP4 OHZ/Royaume-Uni (5 aut.); Uniscan Instruments Ltd./Buxton/Royaume-Uni (6 aut.); Faculté des Sciences et Techniques, Université Hassan II-Mohammedia, B.P. 146/Mohammedia/Maroc (1 aut.); Universidade de Coimbra/3004-535 Coimbra/Portugal (2 aut.); Università di Roma Tor Vergata/00133 Roma/Italie (3 aut.)
DT :	Publication en série; Congrès; Niveau analytique
SO :	Mikrochimica acta : (1966. Print); ISSN 0026-3672; Coden MIACAQ; Allemagne; Da. 2010; Vol. 170; No. 3-4; Pp. 321-330; Bibl. 20 ref.
LA :	Anglais
EA :	Microband biosensors were fabricated from a screen-printed water-based carbon ink containing cobalt phthalocyanine redox mediator and glucose oxidase or lactate oxidase enzyme. The microbiosensors were characterised for their ability to monitor ferrocyanide and H₂O₂ in phosphate buffer solution: sigmoidal cyclic voltammograms, high current density values and steady-state amperometric responses confirmed the existence of radial-diffusion-limiting microelectrode behaviour. The lactate microband biosensors were then used, in conjunction with a screen-printed Ag/AgCl reference and platinum counter electrode, to monitor lactate levels in culture medium, with a linear range of 0.5-5 mM, sensitivity of 20 nA.mM^-1, and dynamic range up to >9 mM. The lactate microband biosensors could operate continuously in culture medium over extended times (up to 24 h) at 37°C. These biosensors were then applied to detect changes in lactate release from cultured cells in response to toxic challenge: m-dinitrobenzene (500 μM) caused a reduction in lactate production by high-passage number HepG2 single cells; D-galactosamine (20 mM) induced release of lactate by HepG2 spheroid cultures. This novel use of microband biosensors in cell culture has the potential for further application in toxicity monitoring, in both environmental and pharmaceutical areas.
CC :	001C04E; 002A31C09B; 001D16E; 001C04A; 215
FD :	Application; Biodétecteur; Environnement; Toxine; Carbone; Cobalt; Enzyme; Solution tampon; Voltammétrie cyclique; Densité élevée; Régime permanent; Ampérométrie; Diffusion; Microélectrode; Eau; Encre; Phtalocyanine; Glucose oxidase; Lactate; Hexacyanoferrate II; Phosphate; Utilisation; Platine; Milieu culture; Sensibilité; Dynamique; Temps; Libération; Benzène(1,3-dinitro); Réduction; Production; Culture cellulaire; Toxicité; Monitorage
FG :	Oxidoreductases
ED :	Application; Biosensor; Environment; Toxin; Carbon; Cobalt; Enzyme; Buffer solution; Cyclic voltammetry; High density; Steady state; Amperometry; Diffusion; Microelectrode; Water; Ink; Phthalocyanine; Glucose oxidase; Lactates; Hexacyanoferrates II; Phosphates; Use; Platinum; Culture medium; Sensitivity; Dynamics; Time; Release; Reduction; Production; Cell culture; Toxicity; Monitoring
EG :	Oxidoreductases
SD :	Aplicación; Biodetector; Medio ambiente; Toxina; Carbono; Cobalto; Enzima; Solución de amortiguación; Voltametría cíclica; Densidad elevada; Régimen permanente; Amperometría; Difusión; Microeléctrodo; Agua; Tinta; Ftalocianina; Glucose oxidase; Lactato; Hexacianoferrato II; Fosfato; Uso; Platino; Medio cultivo; Sensibilidad; Dinámica; Tiempo; Liberación; Reducción; Producción; Cultivo celular; Toxicidad; Monitoreo
LO :	INIST-3977.354000192698520140
ID :	10-0471659

Links to Exploration step

Pascal:10-0471659

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins</title>
<author><name sortKey="Pemberton, Roy M" sort="Pemberton, Roy M" uniqKey="Pemberton R" first="Roy M." last="Pemberton">Roy M. Pemberton</name>
<affiliation><inist:fA14 i1="01"><s1>Centre for Analytical, Materials and Sensors Science, Faculty of Health and Life Sciences, University of the West of England</s1>
<s2>Bristol BS16 1QY</s2>
<s3>GBR</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Rawson, Frankie J" sort="Rawson, Frankie J" uniqKey="Rawson F" first="Frankie J." last="Rawson">Frankie J. Rawson</name>
<affiliation><inist:fA14 i1="01"><s1>Centre for Analytical, Materials and Sensors Science, Faculty of Health and Life Sciences, University of the West of England</s1>
<s2>Bristol BS16 1QY</s2>
<s3>GBR</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Jinsheng Xu" sort="Jinsheng Xu" uniqKey="Jinsheng Xu" last="Jinsheng Xu">JINSHENG XU</name>
<affiliation><inist:fA14 i1="02"><s1>Centre for Research in Biomedicine, Faculty of Health and Life Sciences, University of the West of England</s1>
<s2>Bristol BS16 1QY</s2>
<s3>GBR</s3>
<sZ>3 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Pittson, Robin" sort="Pittson, Robin" uniqKey="Pittson R" first="Robin" last="Pittson">Robin Pittson</name>
<affiliation><inist:fA14 i1="03"><s1>Gwent Electronic Materials Ltd., Monmouth House, Mamhilad Business Park</s1>
<s2>Pontypool NP4 OHZ</s2>
<s3>GBR</s3>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Drago, Guido A" sort="Drago, Guido A" uniqKey="Drago G" first="Guido A." last="Drago">Guido A. Drago</name>
<affiliation><inist:fA14 i1="04"><s1>Applied Enzyme Technology Ltd., Monmouth House, Mamhilad Business Park</s1>
<s2>Pontypool, Gwent NP4 OHZ</s2>
<s3>GBR</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Griffiths, John" sort="Griffiths, John" uniqKey="Griffiths J" first="John" last="Griffiths">John Griffiths</name>
<affiliation><inist:fA14 i1="05"><s1>Uniscan Instruments Ltd.</s1>
<s2>Buxton</s2>
<s3>GBR</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Jackson, Simon K" sort="Jackson, Simon K" uniqKey="Jackson S" first="Simon K." last="Jackson">Simon K. Jackson</name>
<affiliation><inist:fA14 i1="02"><s1>Centre for Research in Biomedicine, Faculty of Health and Life Sciences, University of the West of England</s1>
<s2>Bristol BS16 1QY</s2>
<s3>GBR</s3>
<sZ>3 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Hart, John P" sort="Hart, John P" uniqKey="Hart J" first="John P." last="Hart">John P. Hart</name>
<affiliation><inist:fA14 i1="01"><s1>Centre for Analytical, Materials and Sensors Science, Faculty of Health and Life Sciences, University of the West of England</s1>
<s2>Bristol BS16 1QY</s2>
<s3>GBR</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Mikrochimica acta : (1966. Print)</title>
<title level="j" type="abbreviated">Mikrochim. acta : (1966. Print)</title>
<idno type="ISSN">0026-3672</idno>
<imprint><date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Mikrochimica acta : (1966. Print)</title>
<title level="j" type="abbreviated">Mikrochim. acta : (1966. Print)</title>
<idno type="ISSN">0026-3672</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amperometry</term>
<term>Application</term>
<term>Biosensor</term>
<term>Buffer solution</term>
<term>Carbon</term>
<term>Cell culture</term>
<term>Cobalt</term>
<term>Culture medium</term>
<term>Cyclic voltammetry</term>
<term>Diffusion</term>
<term>Dynamics</term>
<term>Environment</term>
<term>Enzyme</term>
<term>Glucose oxidase</term>
<term>Hexacyanoferrates II</term>
<term>High density</term>
<term>Ink</term>
<term>Lactates</term>
<term>Microelectrode</term>
<term>Monitoring</term>
<term>Phosphates</term>
<term>Phthalocyanine</term>
<term>Platinum</term>
<term>Production</term>
<term>Reduction</term>
<term>Release</term>
<term>Sensitivity</term>
<term>Steady state</term>
<term>Time</term>
<term>Toxicity</term>
<term>Toxin</term>
<term>Use</term>
<term>Water</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Application</term>
<term>Biodétecteur</term>
<term>Environnement</term>
<term>Toxine</term>
<term>Carbone</term>
<term>Cobalt</term>
<term>Enzyme</term>
<term>Solution tampon</term>
<term>Voltammétrie cyclique</term>
<term>Densité élevée</term>
<term>Régime permanent</term>
<term>Ampérométrie</term>
<term>Diffusion</term>
<term>Microélectrode</term>
<term>Eau</term>
<term>Encre</term>
<term>Phtalocyanine</term>
<term>Glucose oxidase</term>
<term>Lactate</term>
<term>Hexacyanoferrate II</term>
<term>Phosphate</term>
<term>Utilisation</term>
<term>Platine</term>
<term>Milieu culture</term>
<term>Sensibilité</term>
<term>Dynamique</term>
<term>Temps</term>
<term>Libération</term>
<term>Benzène(1,3-dinitro)</term>
<term>Réduction</term>
<term>Production</term>
<term>Culture cellulaire</term>
<term>Toxicité</term>
<term>Monitorage</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Microband biosensors were fabricated from a screen-printed water-based carbon ink containing cobalt phthalocyanine redox mediator and glucose oxidase or lactate oxidase enzyme. The microbiosensors were characterised for their ability to monitor ferrocyanide and H<sub>2</sub>
O<sub>2</sub>
 in phosphate buffer solution: sigmoidal cyclic voltammograms, high current density values and steady-state amperometric responses confirmed the existence of radial-diffusion-limiting microelectrode behaviour. The lactate microband biosensors were then used, in conjunction with a screen-printed Ag/AgCl reference and platinum counter electrode, to monitor lactate levels in culture medium, with a linear range of 0.5-5 mM, sensitivity of 20 nA.mM<sup>-1</sup>
, and dynamic range up to >9 mM. The lactate microband biosensors could operate continuously in culture medium over extended times (up to 24 h) at 37°C. These biosensors were then applied to detect changes in lactate release from cultured cells in response to toxic challenge: m-dinitrobenzene (500 μM) caused a reduction in lactate production by high-passage number HepG2 single cells; D-galactosamine (20 mM) induced release of lactate by HepG2 spheroid cultures. This novel use of microband biosensors in cell culture has the potential for further application in toxicity monitoring, in both environmental and pharmaceutical areas.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0026-3672</s0>
</fA01>
<fA02 i1="01"><s0>MIACAQ</s0>
</fA02>
<fA03 i2="1"><s0>Mikrochim. acta : (1966. Print)</s0>
</fA03>
<fA05><s2>170</s2>
</fA05>
<fA06><s2>3-4</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins</s1>
</fA08>
<fA09 i1="01" i2="1" l="ENG"><s1>Biosensors for Food and the Environment</s1>
</fA09>
<fA11 i1="01" i2="1"><s1>PEMBERTON (Roy M.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>RAWSON (Frankie J.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>JINSHENG XU</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>PITTSON (Robin)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>DRAGO (Guido A.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>GRIFFITHS (John)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>JACKSON (Simon K.)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>HART (John P.)</s1>
</fA11>
<fA12 i1="01" i2="1"><s1>AMINE (Aziz)</s1>
<s9>ed.</s9>
</fA12>
<fA12 i1="02" i2="1"><s1>BRETT (Chris M. A.)</s1>
<s9>ed.</s9>
</fA12>
<fA12 i1="03" i2="1"><s1>PALLESCHI (Giuseppe)</s1>
<s9>ed.</s9>
</fA12>
<fA14 i1="01"><s1>Centre for Analytical, Materials and Sensors Science, Faculty of Health and Life Sciences, University of the West of England</s1>
<s2>Bristol BS16 1QY</s2>
<s3>GBR</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Centre for Research in Biomedicine, Faculty of Health and Life Sciences, University of the West of England</s1>
<s2>Bristol BS16 1QY</s2>
<s3>GBR</s3>
<sZ>3 aut.</sZ>
<sZ>7 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Gwent Electronic Materials Ltd., Monmouth House, Mamhilad Business Park</s1>
<s2>Pontypool NP4 OHZ</s2>
<s3>GBR</s3>
<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Applied Enzyme Technology Ltd., Monmouth House, Mamhilad Business Park</s1>
<s2>Pontypool, Gwent NP4 OHZ</s2>
<s3>GBR</s3>
<sZ>5 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>Uniscan Instruments Ltd.</s1>
<s2>Buxton</s2>
<s3>GBR</s3>
<sZ>6 aut.</sZ>
</fA14>
<fA15 i1="01"><s1>Faculté des Sciences et Techniques, Université Hassan II-Mohammedia, B.P. 146</s1>
<s2>Mohammedia</s2>
<s3>MAR</s3>
<sZ>1 aut.</sZ>
</fA15>
<fA15 i1="02"><s1>Universidade de Coimbra</s1>
<s2>3004-535 Coimbra</s2>
<s3>PRT</s3>
<sZ>2 aut.</sZ>
</fA15>
<fA15 i1="03"><s1>Università di Roma Tor Vergata</s1>
<s2>00133 Roma</s2>
<s3>ITA</s3>
<sZ>3 aut.</sZ>
</fA15>
<fA20><s1>321-330</s1>
</fA20>
<fA21><s1>2010</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>3977</s2>
<s5>354000192698520140</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2010 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>20 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>10-0471659</s0>
</fA47>
<fA60><s1>P</s1>
<s2>C</s2>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Mikrochimica acta : (1966. Print)</s0>
</fA64>
<fA66 i1="01"><s0>DEU</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Microband biosensors were fabricated from a screen-printed water-based carbon ink containing cobalt phthalocyanine redox mediator and glucose oxidase or lactate oxidase enzyme. The microbiosensors were characterised for their ability to monitor ferrocyanide and H<sub>2</sub>
O<sub>2</sub>
 in phosphate buffer solution: sigmoidal cyclic voltammograms, high current density values and steady-state amperometric responses confirmed the existence of radial-diffusion-limiting microelectrode behaviour. The lactate microband biosensors were then used, in conjunction with a screen-printed Ag/AgCl reference and platinum counter electrode, to monitor lactate levels in culture medium, with a linear range of 0.5-5 mM, sensitivity of 20 nA.mM<sup>-1</sup>
, and dynamic range up to >9 mM. The lactate microband biosensors could operate continuously in culture medium over extended times (up to 24 h) at 37°C. These biosensors were then applied to detect changes in lactate release from cultured cells in response to toxic challenge: m-dinitrobenzene (500 μM) caused a reduction in lactate production by high-passage number HepG2 single cells; D-galactosamine (20 mM) induced release of lactate by HepG2 spheroid cultures. This novel use of microband biosensors in cell culture has the potential for further application in toxicity monitoring, in both environmental and pharmaceutical areas.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>001C04E</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002A31C09B</s0>
</fC02>
<fC02 i1="03" i2="X"><s0>001D16E</s0>
</fC02>
<fC02 i1="04" i2="X"><s0>001C04A</s0>
</fC02>
<fC02 i1="05" i2="X"><s0>215</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Application</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Application</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Aplicación</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Biodétecteur</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Biosensor</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Biodetector</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Environnement</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Environment</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Medio ambiente</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Toxine</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Toxin</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Toxina</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Carbone</s0>
<s2>NC</s2>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Carbon</s0>
<s2>NC</s2>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Carbono</s0>
<s2>NC</s2>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Cobalt</s0>
<s2>NC</s2>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Cobalt</s0>
<s2>NC</s2>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Cobalto</s0>
<s2>NC</s2>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Solution tampon</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Buffer solution</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Solución de amortiguación</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Voltammétrie cyclique</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Cyclic voltammetry</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Voltametría cíclica</s0>
<s5>09</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Densité élevée</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>High density</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Densidad elevada</s0>
<s5>10</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Régime permanent</s0>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Steady state</s0>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Régimen permanente</s0>
<s5>11</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Ampérométrie</s0>
<s5>12</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Amperometry</s0>
<s5>12</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Amperometría</s0>
<s5>12</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Diffusion</s0>
<s5>13</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Diffusion</s0>
<s5>13</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Difusión</s0>
<s5>13</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Microélectrode</s0>
<s5>14</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Microelectrode</s0>
<s5>14</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Microeléctrodo</s0>
<s5>14</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Eau</s0>
<s5>15</s5>
</fC03>
<fC03 i1="15" i2="X" l="ENG"><s0>Water</s0>
<s5>15</s5>
</fC03>
<fC03 i1="15" i2="X" l="SPA"><s0>Agua</s0>
<s5>15</s5>
</fC03>
<fC03 i1="16" i2="X" l="FRE"><s0>Encre</s0>
<s5>16</s5>
</fC03>
<fC03 i1="16" i2="X" l="ENG"><s0>Ink</s0>
<s5>16</s5>
</fC03>
<fC03 i1="16" i2="X" l="SPA"><s0>Tinta</s0>
<s5>16</s5>
</fC03>
<fC03 i1="17" i2="X" l="FRE"><s0>Phtalocyanine</s0>
<s2>NK</s2>
<s5>17</s5>
</fC03>
<fC03 i1="17" i2="X" l="ENG"><s0>Phthalocyanine</s0>
<s2>NK</s2>
<s5>17</s5>
</fC03>
<fC03 i1="17" i2="X" l="SPA"><s0>Ftalocianina</s0>
<s2>NK</s2>
<s5>17</s5>
</fC03>
<fC03 i1="18" i2="X" l="FRE"><s0>Glucose oxidase</s0>
<s2>FE</s2>
<s5>18</s5>
</fC03>
<fC03 i1="18" i2="X" l="ENG"><s0>Glucose oxidase</s0>
<s2>FE</s2>
<s5>18</s5>
</fC03>
<fC03 i1="18" i2="X" l="SPA"><s0>Glucose oxidase</s0>
<s2>FE</s2>
<s5>18</s5>
</fC03>
<fC03 i1="19" i2="X" l="FRE"><s0>Lactate</s0>
<s5>19</s5>
</fC03>
<fC03 i1="19" i2="X" l="ENG"><s0>Lactates</s0>
<s5>19</s5>
</fC03>
<fC03 i1="19" i2="X" l="SPA"><s0>Lactato</s0>
<s5>19</s5>
</fC03>
<fC03 i1="20" i2="X" l="FRE"><s0>Hexacyanoferrate II</s0>
<s2>NA</s2>
<s5>20</s5>
</fC03>
<fC03 i1="20" i2="X" l="ENG"><s0>Hexacyanoferrates II</s0>
<s2>NA</s2>
<s5>20</s5>
</fC03>
<fC03 i1="20" i2="X" l="SPA"><s0>Hexacianoferrato II</s0>
<s2>NA</s2>
<s5>20</s5>
</fC03>
<fC03 i1="21" i2="X" l="FRE"><s0>Phosphate</s0>
<s2>NA</s2>
<s5>21</s5>
</fC03>
<fC03 i1="21" i2="X" l="ENG"><s0>Phosphates</s0>
<s2>NA</s2>
<s5>21</s5>
</fC03>
<fC03 i1="21" i2="X" l="SPA"><s0>Fosfato</s0>
<s2>NA</s2>
<s5>21</s5>
</fC03>
<fC03 i1="22" i2="X" l="FRE"><s0>Utilisation</s0>
<s5>22</s5>
</fC03>
<fC03 i1="22" i2="X" l="ENG"><s0>Use</s0>
<s5>22</s5>
</fC03>
<fC03 i1="22" i2="X" l="SPA"><s0>Uso</s0>
<s5>22</s5>
</fC03>
<fC03 i1="23" i2="X" l="FRE"><s0>Platine</s0>
<s2>NC</s2>
<s5>23</s5>
</fC03>
<fC03 i1="23" i2="X" l="ENG"><s0>Platinum</s0>
<s2>NC</s2>
<s5>23</s5>
</fC03>
<fC03 i1="23" i2="X" l="SPA"><s0>Platino</s0>
<s2>NC</s2>
<s5>23</s5>
</fC03>
<fC03 i1="24" i2="X" l="FRE"><s0>Milieu culture</s0>
<s5>24</s5>
</fC03>
<fC03 i1="24" i2="X" l="ENG"><s0>Culture medium</s0>
<s5>24</s5>
</fC03>
<fC03 i1="24" i2="X" l="SPA"><s0>Medio cultivo</s0>
<s5>24</s5>
</fC03>
<fC03 i1="25" i2="X" l="FRE"><s0>Sensibilité</s0>
<s5>25</s5>
</fC03>
<fC03 i1="25" i2="X" l="ENG"><s0>Sensitivity</s0>
<s5>25</s5>
</fC03>
<fC03 i1="25" i2="X" l="SPA"><s0>Sensibilidad</s0>
<s5>25</s5>
</fC03>
<fC03 i1="26" i2="X" l="FRE"><s0>Dynamique</s0>
<s5>26</s5>
</fC03>
<fC03 i1="26" i2="X" l="ENG"><s0>Dynamics</s0>
<s5>26</s5>
</fC03>
<fC03 i1="26" i2="X" l="SPA"><s0>Dinámica</s0>
<s5>26</s5>
</fC03>
<fC03 i1="27" i2="X" l="FRE"><s0>Temps</s0>
<s5>27</s5>
</fC03>
<fC03 i1="27" i2="X" l="ENG"><s0>Time</s0>
<s5>27</s5>
</fC03>
<fC03 i1="27" i2="X" l="SPA"><s0>Tiempo</s0>
<s5>27</s5>
</fC03>
<fC03 i1="28" i2="X" l="FRE"><s0>Libération</s0>
<s5>28</s5>
</fC03>
<fC03 i1="28" i2="X" l="ENG"><s0>Release</s0>
<s5>28</s5>
</fC03>
<fC03 i1="28" i2="X" l="SPA"><s0>Liberación</s0>
<s5>28</s5>
</fC03>
<fC03 i1="29" i2="X" l="FRE"><s0>Benzène(1,3-dinitro)</s0>
<s2>NK</s2>
<s2>FX</s2>
<s5>29</s5>
</fC03>
<fC03 i1="30" i2="X" l="FRE"><s0>Réduction</s0>
<s5>30</s5>
</fC03>
<fC03 i1="30" i2="X" l="ENG"><s0>Reduction</s0>
<s5>30</s5>
</fC03>
<fC03 i1="30" i2="X" l="SPA"><s0>Reducción</s0>
<s5>30</s5>
</fC03>
<fC03 i1="31" i2="X" l="FRE"><s0>Production</s0>
<s5>41</s5>
</fC03>
<fC03 i1="31" i2="X" l="ENG"><s0>Production</s0>
<s5>41</s5>
</fC03>
<fC03 i1="31" i2="X" l="SPA"><s0>Producción</s0>
<s5>41</s5>
</fC03>
<fC03 i1="32" i2="X" l="FRE"><s0>Culture cellulaire</s0>
<s5>42</s5>
</fC03>
<fC03 i1="32" i2="X" l="ENG"><s0>Cell culture</s0>
<s5>42</s5>
</fC03>
<fC03 i1="32" i2="X" l="SPA"><s0>Cultivo celular</s0>
<s5>42</s5>
</fC03>
<fC03 i1="33" i2="X" l="FRE"><s0>Toxicité</s0>
<s5>43</s5>
</fC03>
<fC03 i1="33" i2="X" l="ENG"><s0>Toxicity</s0>
<s5>43</s5>
</fC03>
<fC03 i1="33" i2="X" l="SPA"><s0>Toxicidad</s0>
<s5>43</s5>
</fC03>
<fC03 i1="34" i2="X" l="FRE"><s0>Monitorage</s0>
<s5>44</s5>
</fC03>
<fC03 i1="34" i2="X" l="ENG"><s0>Monitoring</s0>
<s5>44</s5>
</fC03>
<fC03 i1="34" i2="X" l="SPA"><s0>Monitoreo</s0>
<s5>44</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Oxidoreductases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Oxidoreductases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Oxidoreductases</s0>
<s2>FE</s2>
</fC07>
<fN21><s1>305</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
<pR><fA30 i1="01" i2="1" l="ENG"><s1>International Workshop on Biosensors for Food Safety and Environmental Monitoring</s1>
<s2>4</s2>
<s3>Tangier MAR</s3>
<s4>2009-10-01</s4>
</fA30>
</pR>
</standard>
<server><NO>PASCAL 10-0471659 INIST</NO>
<ET>Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins</ET>
<AU>PEMBERTON (Roy M.); RAWSON (Frankie J.); JINSHENG XU; PITTSON (Robin); DRAGO (Guido A.); GRIFFITHS (John); JACKSON (Simon K.); HART (John P.); AMINE (Aziz); BRETT (Chris M. A.); PALLESCHI (Giuseppe)</AU>
<AF>Centre for Analytical, Materials and Sensors Science, Faculty of Health and Life Sciences, University of the West of England/Bristol BS16 1QY/Royaume-Uni (1 aut., 2 aut., 8 aut.); Centre for Research in Biomedicine, Faculty of Health and Life Sciences, University of the West of England/Bristol BS16 1QY/Royaume-Uni (3 aut., 7 aut.); Gwent Electronic Materials Ltd., Monmouth House, Mamhilad Business Park/Pontypool NP4 OHZ/Royaume-Uni (4 aut.); Applied Enzyme Technology Ltd., Monmouth House, Mamhilad Business Park/Pontypool, Gwent NP4 OHZ/Royaume-Uni (5 aut.); Uniscan Instruments Ltd./Buxton/Royaume-Uni (6 aut.); Faculté des Sciences et Techniques, Université Hassan II-Mohammedia, B.P. 146/Mohammedia/Maroc (1 aut.); Universidade de Coimbra/3004-535 Coimbra/Portugal (2 aut.); Università di Roma Tor Vergata/00133 Roma/Italie (3 aut.)</AF>
<DT>Publication en série; Congrès; Niveau analytique</DT>
<SO>Mikrochimica acta : (1966. Print); ISSN 0026-3672; Coden MIACAQ; Allemagne; Da. 2010; Vol. 170; No. 3-4; Pp. 321-330; Bibl. 20 ref.</SO>
<LA>Anglais</LA>
<EA>Microband biosensors were fabricated from a screen-printed water-based carbon ink containing cobalt phthalocyanine redox mediator and glucose oxidase or lactate oxidase enzyme. The microbiosensors were characterised for their ability to monitor ferrocyanide and H<sub>2</sub>
O<sub>2</sub>
 in phosphate buffer solution: sigmoidal cyclic voltammograms, high current density values and steady-state amperometric responses confirmed the existence of radial-diffusion-limiting microelectrode behaviour. The lactate microband biosensors were then used, in conjunction with a screen-printed Ag/AgCl reference and platinum counter electrode, to monitor lactate levels in culture medium, with a linear range of 0.5-5 mM, sensitivity of 20 nA.mM<sup>-1</sup>
, and dynamic range up to >9 mM. The lactate microband biosensors could operate continuously in culture medium over extended times (up to 24 h) at 37°C. These biosensors were then applied to detect changes in lactate release from cultured cells in response to toxic challenge: m-dinitrobenzene (500 μM) caused a reduction in lactate production by high-passage number HepG2 single cells; D-galactosamine (20 mM) induced release of lactate by HepG2 spheroid cultures. This novel use of microband biosensors in cell culture has the potential for further application in toxicity monitoring, in both environmental and pharmaceutical areas.</EA>
<CC>001C04E; 002A31C09B; 001D16E; 001C04A; 215</CC>
<FD>Application; Biodétecteur; Environnement; Toxine; Carbone; Cobalt; Enzyme; Solution tampon; Voltammétrie cyclique; Densité élevée; Régime permanent; Ampérométrie; Diffusion; Microélectrode; Eau; Encre; Phtalocyanine; Glucose oxidase; Lactate; Hexacyanoferrate II; Phosphate; Utilisation; Platine; Milieu culture; Sensibilité; Dynamique; Temps; Libération; Benzène(1,3-dinitro); Réduction; Production; Culture cellulaire; Toxicité; Monitorage</FD>
<FG>Oxidoreductases</FG>
<ED>Application; Biosensor; Environment; Toxin; Carbon; Cobalt; Enzyme; Buffer solution; Cyclic voltammetry; High density; Steady state; Amperometry; Diffusion; Microelectrode; Water; Ink; Phthalocyanine; Glucose oxidase; Lactates; Hexacyanoferrates II; Phosphates; Use; Platinum; Culture medium; Sensitivity; Dynamics; Time; Release; Reduction; Production; Cell culture; Toxicity; Monitoring</ED>
<EG>Oxidoreductases</EG>
<SD>Aplicación; Biodetector; Medio ambiente; Toxina; Carbono; Cobalto; Enzima; Solución de amortiguación; Voltametría cíclica; Densidad elevada; Régimen permanente; Amperometría; Difusión; Microeléctrodo; Agua; Tinta; Ftalocianina; Glucose oxidase; Lactato; Hexacianoferrato II; Fosfato; Uso; Platino; Medio cultivo; Sensibilidad; Dinámica; Tiempo; Liberación; Reducción; Producción; Cultivo celular; Toxicidad; Monitoreo</SD>
<LO>INIST-3977.354000192698520140</LO>
<ID>10-0471659</ID>
</server>
</inist>
</record>

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	Serveur d'exploration sur le cobalt au Maghreb
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Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins

Application of screen-printed microband biosensors incorporated with cells to monitor metabolic effects of potential environmental toxins

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