Studies towards an amperometric phosphate ion biosensor for urine and water analysis
Identifieur interne : 000080 ( PascalFrancis/Checkpoint ); précédent : 000079; suivant : 000081Studies towards an amperometric phosphate ion biosensor for urine and water analysis
Auteurs : Lucy Gilbert [Royaume-Uni] ; Simon Browning [Royaume-Uni] ; Andrew T. A. Jenkins [Royaume-Uni] ; John P. Hart [Royaume-Uni]Source :
- Mikrochimica acta : (1966. Print) [ 0026-3672 ] ; 2010.
Descripteurs français
- Pascal (Inist)
- Ampérométrie, Biodétecteur, Cobalt, Electrode carbone, Enzyme, Transducteur, Electrocatalyse, Oxydation, Signal, Immobilisation, Membrane, In situ, Cellulose acétate, Cofacteur, Phosphate, Urine, Eau distribution, Phtalocyanine, Peroxyde d'hydrogène, Dioxyde de carbone, Acétone, Caractéristique courant tension, Etalonnage, Echantillon, Application, Contrôle milieu ambiant, Capteur chimique.
- Wicri :
English descriptors
- KwdEn :
- Acetone, Amperometry, Application, Biosensor, Calibration, Carbon dioxide, Carbon electrode, Cellulose acetate, Chemical sensor, Cobalt, Cofactor, Electrocatalysis, Environmental control, Enzyme, Hydrogen peroxide, Immobilization, In situ, Membrane, Oxidation, Phosphates, Phthalocyanine, Sample, Signal, Tap water, Transducer, Urine, Voltage current curve.
Abstract
An amperometric biosensor for phosphate ion is described that is based on a cobalt phthalocyanine modified screen-printed carbon electrode (CoPC-SPCE). The biosensor operation is based on the enzyme pyruvate oxidase (PyOd) which catalyses the oxidative decarboxylation of pyruvate, in the presence of inorganic phosphate and O2, to acetyl phosphate, hydrogen peroxide (H2O2) and CO2. The transducer allows the electrocatalytic oxidation of H2O2 in order to generate the analytical signal. The enzyme was immobilised onto the CoPC-SPCE using a sandwich format. The inner membrane was formed in situ by depositing an acetone solution containing cellulose acetate first onto the transducer surface. The enzyme and cofactors were then deposited onto this layer and allowed to dry; finally a second aliquot of the cellulose acetate solution was deposited onto the enzyme layer and allowed to dry. The biosensor was characterised by amperometry in stirred solution to produce current-voltage curves and for calibration studies. From these it was deduced that a reliable electrocatalytic response was obtained for phosphate ion; an operating potential of +0.4 V was selected for the analysis of urine samples. The precision of the response for urine analysis and recovery data for potable water suggests that the biosensor could have applications in clinical and environmental monitoring.
Affiliations:
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Pascal:10-0471660Le document en format XML
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A." last="Jenkins">Andrew T. A. Jenkins</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Chemistry Department, University of Bath</s1><s2>Bath BA2 7AY</s2><s3>GBR</s3><sZ>3 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Bath BA2 7AY</wicri:noRegion></affiliation></author><author><name sortKey="Hart, John P" sort="Hart, John P" uniqKey="Hart J" first="John P." last="Hart">John P. Hart</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Centre for Research in Analytical, Materials and Sensor Science, University of the West of England</s1><s2>Bristol BS16 1QY</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Bristol BS16 1QY</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Mikrochimica acta : (1966. Print)</title><title level="j" type="abbreviated">Mikrochim. acta : (1966. Print)</title><idno type="ISSN">0026-3672</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Mikrochimica acta : (1966. Print)</title><title level="j" type="abbreviated">Mikrochim. acta : (1966. Print)</title><idno type="ISSN">0026-3672</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetone</term><term>Amperometry</term><term>Application</term><term>Biosensor</term><term>Calibration</term><term>Carbon dioxide</term><term>Carbon electrode</term><term>Cellulose acetate</term><term>Chemical sensor</term><term>Cobalt</term><term>Cofactor</term><term>Electrocatalysis</term><term>Environmental control</term><term>Enzyme</term><term>Hydrogen peroxide</term><term>Immobilization</term><term>In situ</term><term>Membrane</term><term>Oxidation</term><term>Phosphates</term><term>Phthalocyanine</term><term>Sample</term><term>Signal</term><term>Tap water</term><term>Transducer</term><term>Urine</term><term>Voltage current curve</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Ampérométrie</term><term>Biodétecteur</term><term>Cobalt</term><term>Electrode carbone</term><term>Enzyme</term><term>Transducteur</term><term>Electrocatalyse</term><term>Oxydation</term><term>Signal</term><term>Immobilisation</term><term>Membrane</term><term>In situ</term><term>Cellulose acétate</term><term>Cofacteur</term><term>Phosphate</term><term>Urine</term><term>Eau distribution</term><term>Phtalocyanine</term><term>Peroxyde d'hydrogène</term><term>Dioxyde de carbone</term><term>Acétone</term><term>Caractéristique courant tension</term><term>Etalonnage</term><term>Echantillon</term><term>Application</term><term>Contrôle milieu ambiant</term><term>Capteur chimique</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Cobalt</term><term>Enzyme</term><term>Phosphate</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An amperometric biosensor for phosphate ion is described that is based on a cobalt phthalocyanine modified screen-printed carbon electrode (CoPC-SPCE). The biosensor operation is based on the enzyme pyruvate oxidase (PyOd) which catalyses the oxidative decarboxylation of pyruvate, in the presence of inorganic phosphate and O<sub>2</sub>, to acetyl phosphate, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and CO<sub>2</sub>. The transducer allows the electrocatalytic oxidation of H<sub>2</sub>O<sub>2</sub> in order to generate the analytical signal. The enzyme was immobilised onto the CoPC-SPCE using a sandwich format. The inner membrane was formed in situ by depositing an acetone solution containing cellulose acetate first onto the transducer surface. The enzyme and cofactors were then deposited onto this layer and allowed to dry; finally a second aliquot of the cellulose acetate solution was deposited onto the enzyme layer and allowed to dry. The biosensor was characterised by amperometry in stirred solution to produce current-voltage curves and for calibration studies. From these it was deduced that a reliable electrocatalytic response was obtained for phosphate ion; an operating potential of +0.4 V was selected for the analysis of urine samples. The precision of the response for urine analysis and recovery data for potable water suggests that the biosensor could have applications in clinical and environmental monitoring.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0026-3672</s0></fA01><fA02 i1="01"><s0>MIACAQ</s0></fA02><fA03 i2="1"><s0>Mikrochim. acta : (1966. Print)</s0></fA03><fA05><s2>170</s2></fA05><fA06><s2>3-4</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Studies towards an amperometric phosphate ion biosensor for urine and water analysis</s1></fA08><fA09 i1="01" i2="1" l="ENG"><s1>Biosensors for Food and the Environment</s1></fA09><fA11 i1="01" i2="1"><s1>GILBERT (Lucy)</s1></fA11><fA11 i1="02" i2="1"><s1>BROWNING (Simon)</s1></fA11><fA11 i1="03" i2="1"><s1>JENKINS (Andrew T. A.)</s1></fA11><fA11 i1="04" i2="1"><s1>HART (John P.)</s1></fA11><fA12 i1="01" i2="1"><s1>AMINE (Aziz)</s1><s9>ed.</s9></fA12><fA12 i1="02" i2="1"><s1>BRETT (Chris M. A.)</s1><s9>ed.</s9></fA12><fA12 i1="03" i2="1"><s1>PALLESCHI (Giuseppe)</s1><s9>ed.</s9></fA12><fA14 i1="01"><s1>Centre for Research in Analytical, Materials and Sensor Science, University of the West of England</s1><s2>Bristol BS16 1QY</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ></fA14><fA14 i1="02"><s1>Environment Agency, Rivers House, Lower Bristol Road</s1><s2>Bath BA2 9ES</s2><s3>GBR</s3><sZ>2 aut.</sZ></fA14><fA14 i1="03"><s1>Chemistry Department, University of Bath</s1><s2>Bath BA2 7AY</s2><s3>GBR</s3><sZ>3 aut.</sZ></fA14><fA15 i1="01"><s1>Faculté des Sciences et Techniques, Université Hassan II-Mohammedia, B.P. 146</s1><s2>Mohammedia</s2><s3>MAR</s3><sZ>1 aut.</sZ></fA15><fA15 i1="02"><s1>Universidade de Coimbra</s1><s2>3004-535 Coimbra</s2><s3>PRT</s3><sZ>2 aut.</sZ></fA15><fA15 i1="03"><s1>Università di Roma Tor Vergata</s1><s2>00133 Roma</s2><s3>ITA</s3><sZ>3 aut.</sZ></fA15><fA20><s1>331-336</s1></fA20><fA21><s1>2010</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>3977</s2><s5>354000192698520150</s5></fA43><fA44><s0>0000</s0><s1>© 2010 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>29 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>10-0471660</s0></fA47><fA60><s1>P</s1><s2>C</s2></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Mikrochimica acta : (1966. Print)</s0></fA64><fA66 i1="01"><s0>DEU</s0></fA66><fC01 i1="01" l="ENG"><s0>An amperometric biosensor for phosphate ion is described that is based on a cobalt phthalocyanine modified screen-printed carbon electrode (CoPC-SPCE). The biosensor operation is based on the enzyme pyruvate oxidase (PyOd) which catalyses the oxidative decarboxylation of pyruvate, in the presence of inorganic phosphate and O<sub>2</sub>, to acetyl phosphate, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and CO<sub>2</sub>. The transducer allows the electrocatalytic oxidation of H<sub>2</sub>O<sub>2</sub> in order to generate the analytical signal. The enzyme was immobilised onto the CoPC-SPCE using a sandwich format. The inner membrane was formed in situ by depositing an acetone solution containing cellulose acetate first onto the transducer surface. The enzyme and cofactors were then deposited onto this layer and allowed to dry; finally a second aliquot of the cellulose acetate solution was deposited onto the enzyme layer and allowed to dry. The biosensor was characterised by amperometry in stirred solution to produce current-voltage curves and for calibration studies. From these it was deduced that a reliable electrocatalytic response was obtained for phosphate ion; an operating potential of +0.4 V was selected for the analysis of urine samples. The precision of the response for urine analysis and recovery data for potable water suggests that the biosensor could have applications in clinical and environmental monitoring.</s0></fC01><fC02 i1="01" i2="X"><s0>001C04E</s0></fC02><fC02 i1="02" i2="X"><s0>002A31C09B</s0></fC02><fC02 i1="03" i2="X"><s0>001D16E</s0></fC02><fC02 i1="04" i2="X"><s0>001C04A</s0></fC02><fC02 i1="05" i2="X"><s0>215</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Ampérométrie</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Amperometry</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Amperometría</s0><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Biodétecteur</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Biosensor</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Biodetector</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Cobalt</s0><s2>NC</s2><s5>03</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Cobalt</s0><s2>NC</s2><s5>03</s5></fC03><fC03 i1="03" i2="X" 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l="ENG"><s0>Cellulose acetate</s0><s2>NK</s2><s5>13</s5></fC03><fC03 i1="13" i2="X" l="SPA"><s0>Celulosa acetato</s0><s2>NK</s2><s5>13</s5></fC03><fC03 i1="14" i2="X" l="FRE"><s0>Cofacteur</s0><s5>14</s5></fC03><fC03 i1="14" i2="X" l="ENG"><s0>Cofactor</s0><s5>14</s5></fC03><fC03 i1="14" i2="X" l="SPA"><s0>Cofactor</s0><s5>14</s5></fC03><fC03 i1="15" i2="X" l="FRE"><s0>Phosphate</s0><s2>NA</s2><s5>15</s5></fC03><fC03 i1="15" i2="X" l="ENG"><s0>Phosphates</s0><s2>NA</s2><s5>15</s5></fC03><fC03 i1="15" i2="X" l="SPA"><s0>Fosfato</s0><s2>NA</s2><s5>15</s5></fC03><fC03 i1="16" i2="X" l="FRE"><s0>Urine</s0><s5>16</s5></fC03><fC03 i1="16" i2="X" l="ENG"><s0>Urine</s0><s5>16</s5></fC03><fC03 i1="16" i2="X" l="SPA"><s0>Orina</s0><s5>16</s5></fC03><fC03 i1="17" i2="X" l="FRE"><s0>Eau distribution</s0><s5>17</s5></fC03><fC03 i1="17" i2="X" l="ENG"><s0>Tap water</s0><s5>17</s5></fC03><fC03 i1="17" i2="X" l="SPA"><s0>Agua distribución</s0><s5>17</s5></fC03><fC03 i1="18" i2="X" l="FRE"><s0>Phtalocyanine</s0><s2>NK</s2><s5>18</s5></fC03><fC03 i1="18" i2="X" l="ENG"><s0>Phthalocyanine</s0><s2>NK</s2><s5>18</s5></fC03><fC03 i1="18" i2="X" l="SPA"><s0>Ftalocianina</s0><s2>NK</s2><s5>18</s5></fC03><fC03 i1="19" i2="X" l="FRE"><s0>Peroxyde d'hydrogène</s0><s2>NK</s2><s5>19</s5></fC03><fC03 i1="19" i2="X" l="ENG"><s0>Hydrogen peroxide</s0><s2>NK</s2><s5>19</s5></fC03><fC03 i1="19" i2="X" l="SPA"><s0>Peróxido de hydrogeno</s0><s2>NK</s2><s5>19</s5></fC03><fC03 i1="20" i2="X" l="FRE"><s0>Dioxyde de carbone</s0><s2>NK</s2><s2>FX</s2><s5>20</s5></fC03><fC03 i1="20" i2="X" l="ENG"><s0>Carbon dioxide</s0><s2>NK</s2><s2>FX</s2><s5>20</s5></fC03><fC03 i1="20" i2="X" l="SPA"><s0>Carbono dióxido</s0><s2>NK</s2><s2>FX</s2><s5>20</s5></fC03><fC03 i1="21" i2="X" l="FRE"><s0>Acétone</s0><s2>NK</s2><s2>FX</s2><s5>21</s5></fC03><fC03 i1="21" i2="X" l="ENG"><s0>Acetone</s0><s2>NK</s2><s2>FX</s2><s5>21</s5></fC03><fC03 i1="21" i2="X" l="SPA"><s0>Acetona</s0><s2>NK</s2><s2>FX</s2><s5>21</s5></fC03><fC03 i1="22" i2="X" l="FRE"><s0>Caractéristique courant tension</s0><s5>22</s5></fC03><fC03 i1="22" i2="X" l="ENG"><s0>Voltage current curve</s0><s5>22</s5></fC03><fC03 i1="22" i2="X" l="SPA"><s0>Característica corriente tensión</s0><s5>22</s5></fC03><fC03 i1="23" i2="X" l="FRE"><s0>Etalonnage</s0><s5>23</s5></fC03><fC03 i1="23" i2="X" l="ENG"><s0>Calibration</s0><s5>23</s5></fC03><fC03 i1="23" i2="X" l="SPA"><s0>Contraste</s0><s5>23</s5></fC03><fC03 i1="24" i2="X" l="FRE"><s0>Echantillon</s0><s5>24</s5></fC03><fC03 i1="24" i2="X" l="ENG"><s0>Sample</s0><s5>24</s5></fC03><fC03 i1="24" i2="X" l="SPA"><s0>Muestra</s0><s5>24</s5></fC03><fC03 i1="25" i2="X" l="FRE"><s0>Application</s0><s5>25</s5></fC03><fC03 i1="25" i2="X" l="ENG"><s0>Application</s0><s5>25</s5></fC03><fC03 i1="25" i2="X" l="SPA"><s0>Aplicación</s0><s5>25</s5></fC03><fC03 i1="26" i2="X" l="FRE"><s0>Contrôle milieu ambiant</s0><s5>26</s5></fC03><fC03 i1="26" i2="X" l="ENG"><s0>Environmental control</s0><s5>26</s5></fC03><fC03 i1="26" i2="X" l="SPA"><s0>Control medio ambiante</s0><s5>26</s5></fC03><fC03 i1="27" i2="X" l="FRE"><s0>Capteur chimique</s0><s5>27</s5></fC03><fC03 i1="27" i2="X" l="ENG"><s0>Chemical sensor</s0><s5>27</s5></fC03><fC03 i1="27" i2="X" l="SPA"><s0>Captador químico</s0><s5>27</s5></fC03><fN21><s1>305</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA><pR><fA30 i1="01" i2="1" l="ENG"><s1>International Workshop on Biosensors for Food Safety and Environmental Monitoring</s1><s2>4</s2><s3>Tangier MAR</s3><s4>2009-10-01</s4></fA30></pR></standard></inist><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Gilbert, Lucy" sort="Gilbert, Lucy" uniqKey="Gilbert L" first="Lucy" last="Gilbert">Lucy Gilbert</name></noRegion><name sortKey="Browning, Simon" sort="Browning, Simon" uniqKey="Browning S" first="Simon" last="Browning">Simon Browning</name><name sortKey="Hart, John P" sort="Hart, John P" uniqKey="Hart J" first="John P." last="Hart">John P. Hart</name><name sortKey="Jenkins, Andrew T A" sort="Jenkins, Andrew T A" uniqKey="Jenkins A" first="Andrew T. A." last="Jenkins">Andrew T. A. Jenkins</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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