Pathophysiological effects of Cerastes cerastes and Vipera lebetina venoms: Immunoneutralization using anti-native and anti-(60)Co irradiated venoms.
Identifieur interne : 000426 ( Ncbi/Checkpoint ); précédent : 000425; suivant : 000427Pathophysiological effects of Cerastes cerastes and Vipera lebetina venoms: Immunoneutralization using anti-native and anti-(60)Co irradiated venoms.
Auteurs : Sabrina Boumaiza [Algérie] ; Habiba Oussedik-Oumehdi [Algérie] ; Fatima Laraba-Djebari [Algérie]Source :
- Biologicals : journal of the International Association of Biological Standardization [ 1095-8320 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- antagonistes et inhibiteurs : Venins de vipère.
- pharmacologie : Sérums antivenimeux.
- toxicité : Venins de vipère.
- Animaux, Radio-isotopes du cobalt, Rayons gamma, Souris, Sérums antivenimeux, Viperidae.
English descriptors
- KwdEn :
- MESH :
- chemical , antagonists & inhibitors : Viper Venoms.
- chemical , chemistry : Antivenins.
- chemical , pharmacology : Antivenins.
- chemical , toxicity : Viper Venoms.
- Animals, Cobalt Radioisotopes, Gamma Rays, Mice, Viperidae.
Abstract
Cerastes cerastes and Vipera lebetina are the most medically important vipers in Algeria. Their bite induces several pathological effects on victims of accidental envenomation. In this study we analyzed the pathogenesis induced after an experimental envenomation. Indeed, we determined, in vitro, venom enzymatic activities and we analyzed, in vivo, pathological effects induced on liver, heart, lung and skin. In addition we investigated the neutralizing potency of four experimental antivenoms elicited against native and irradiated venoms. Results revealed that V. lebetina and Cerastes cerastes venoms presented strong hemorrhagic, oedematic and necrotic activities. Histopathological study showed that both venoms induced deep damage in tissue structures leading to organ dysfunction. They also increased cellular peroxidases activities, indicating an inflammatory process that is known to amplify tissue damage. Western-blot analysis evidenced that anti-irradiated venoms recognized most components of native venoms. Antivenoms were effective in neutralizing all tested activities, with an increased protective effect obtained with anti-irradiated venoms. Anti-irradiated venoms reduced cellular peroxidases activities indicating a reduction of the inflammatory response. These results may improve our understanding of Algerian Viperidae bite pathogenesis and would encourage further studies planning to provide more proofs on the effectiveness of anti-irradiated venoms administration in the treatment of envenomation.
DOI: 10.1016/j.biologicals.2015.11.002
PubMed: 26678662
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000008
- to stream PubMed, to step Curation: 000008
- to stream PubMed, to step Checkpoint: 000008
- to stream Ncbi, to step Merge: 000426
- to stream Ncbi, to step Curation: 000426
Links to Exploration step
pubmed:26678662Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Pathophysiological effects of Cerastes cerastes and Vipera lebetina venoms: Immunoneutralization using anti-native and anti-(60)Co irradiated venoms.</title>
<author><name sortKey="Boumaiza, Sabrina" sort="Boumaiza, Sabrina" uniqKey="Boumaiza S" first="Sabrina" last="Boumaiza">Sabrina Boumaiza</name>
<affiliation wicri:level="3"><nlm:affiliation>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers, Algeria.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers</wicri:regionArea>
<placeName><settlement type="city">Alger</settlement>
<region nuts="2">Wilaya d'Alger</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Oussedik Oumehdi, Habiba" sort="Oussedik Oumehdi, Habiba" uniqKey="Oussedik Oumehdi H" first="Habiba" last="Oussedik-Oumehdi">Habiba Oussedik-Oumehdi</name>
<affiliation wicri:level="3"><nlm:affiliation>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers, Algeria.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers</wicri:regionArea>
<placeName><settlement type="city">Alger</settlement>
<region nuts="2">Wilaya d'Alger</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Laraba Djebari, Fatima" sort="Laraba Djebari, Fatima" uniqKey="Laraba Djebari F" first="Fatima" last="Laraba-Djebari">Fatima Laraba-Djebari</name>
<affiliation wicri:level="3"><nlm:affiliation>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers, Algeria. Electronic address: flaraba@hotmail.com.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers</wicri:regionArea>
<placeName><settlement type="city">Alger</settlement>
<region nuts="2">Wilaya d'Alger</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2016">2016</date>
<idno type="RBID">pubmed:26678662</idno>
<idno type="pmid">26678662</idno>
<idno type="doi">10.1016/j.biologicals.2015.11.002</idno>
<idno type="wicri:Area/PubMed/Corpus">000008</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000008</idno>
<idno type="wicri:Area/PubMed/Curation">000008</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000008</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000008</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000008</idno>
<idno type="wicri:Area/Ncbi/Merge">000426</idno>
<idno type="wicri:Area/Ncbi/Curation">000426</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000426</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Pathophysiological effects of Cerastes cerastes and Vipera lebetina venoms: Immunoneutralization using anti-native and anti-(60)Co irradiated venoms.</title>
<author><name sortKey="Boumaiza, Sabrina" sort="Boumaiza, Sabrina" uniqKey="Boumaiza S" first="Sabrina" last="Boumaiza">Sabrina Boumaiza</name>
<affiliation wicri:level="3"><nlm:affiliation>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers, Algeria.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers</wicri:regionArea>
<placeName><settlement type="city">Alger</settlement>
<region nuts="2">Wilaya d'Alger</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Oussedik Oumehdi, Habiba" sort="Oussedik Oumehdi, Habiba" uniqKey="Oussedik Oumehdi H" first="Habiba" last="Oussedik-Oumehdi">Habiba Oussedik-Oumehdi</name>
<affiliation wicri:level="3"><nlm:affiliation>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers, Algeria.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers</wicri:regionArea>
<placeName><settlement type="city">Alger</settlement>
<region nuts="2">Wilaya d'Alger</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Laraba Djebari, Fatima" sort="Laraba Djebari, Fatima" uniqKey="Laraba Djebari F" first="Fatima" last="Laraba-Djebari">Fatima Laraba-Djebari</name>
<affiliation wicri:level="3"><nlm:affiliation>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers, Algeria. Electronic address: flaraba@hotmail.com.</nlm:affiliation>
<country xml:lang="fr">Algérie</country>
<wicri:regionArea>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers</wicri:regionArea>
<placeName><settlement type="city">Alger</settlement>
<region nuts="2">Wilaya d'Alger</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Biologicals : journal of the International Association of Biological Standardization</title>
<idno type="eISSN">1095-8320</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antivenins (chemistry)</term>
<term>Antivenins (pharmacology)</term>
<term>Cobalt Radioisotopes</term>
<term>Gamma Rays</term>
<term>Mice</term>
<term>Viper Venoms (antagonists & inhibitors)</term>
<term>Viper Venoms (toxicity)</term>
<term>Viperidae</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Radio-isotopes du cobalt</term>
<term>Rayons gamma</term>
<term>Souris</term>
<term>Sérums antivenimeux ()</term>
<term>Sérums antivenimeux (pharmacologie)</term>
<term>Venins de vipère (antagonistes et inhibiteurs)</term>
<term>Venins de vipère (toxicité)</term>
<term>Viperidae</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Viper Venoms</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antivenins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antivenins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Viper Venoms</term>
</keywords>
<keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Venins de vipère</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Sérums antivenimeux</term>
</keywords>
<keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Venins de vipère</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cobalt Radioisotopes</term>
<term>Gamma Rays</term>
<term>Mice</term>
<term>Viperidae</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Radio-isotopes du cobalt</term>
<term>Rayons gamma</term>
<term>Souris</term>
<term>Sérums antivenimeux</term>
<term>Viperidae</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Cerastes cerastes and Vipera lebetina are the most medically important vipers in Algeria. Their bite induces several pathological effects on victims of accidental envenomation. In this study we analyzed the pathogenesis induced after an experimental envenomation. Indeed, we determined, in vitro, venom enzymatic activities and we analyzed, in vivo, pathological effects induced on liver, heart, lung and skin. In addition we investigated the neutralizing potency of four experimental antivenoms elicited against native and irradiated venoms. Results revealed that V. lebetina and Cerastes cerastes venoms presented strong hemorrhagic, oedematic and necrotic activities. Histopathological study showed that both venoms induced deep damage in tissue structures leading to organ dysfunction. They also increased cellular peroxidases activities, indicating an inflammatory process that is known to amplify tissue damage. Western-blot analysis evidenced that anti-irradiated venoms recognized most components of native venoms. Antivenoms were effective in neutralizing all tested activities, with an increased protective effect obtained with anti-irradiated venoms. Anti-irradiated venoms reduced cellular peroxidases activities indicating a reduction of the inflammatory response. These results may improve our understanding of Algerian Viperidae bite pathogenesis and would encourage further studies planning to provide more proofs on the effectiveness of anti-irradiated venoms administration in the treatment of envenomation.</div>
</front>
</TEI>
<affiliations><list><country><li>Algérie</li>
</country>
<region><li>Wilaya d'Alger</li>
</region>
<settlement><li>Alger</li>
</settlement>
</list>
<tree><country name="Algérie"><region name="Wilaya d'Alger"><name sortKey="Boumaiza, Sabrina" sort="Boumaiza, Sabrina" uniqKey="Boumaiza S" first="Sabrina" last="Boumaiza">Sabrina Boumaiza</name>
</region>
<name sortKey="Laraba Djebari, Fatima" sort="Laraba Djebari, Fatima" uniqKey="Laraba Djebari F" first="Fatima" last="Laraba-Djebari">Fatima Laraba-Djebari</name>
<name sortKey="Oussedik Oumehdi, Habiba" sort="Oussedik Oumehdi, Habiba" uniqKey="Oussedik Oumehdi H" first="Habiba" last="Oussedik-Oumehdi">Habiba Oussedik-Oumehdi</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/CobaltMaghrebV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000426 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000426 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Terre |area= CobaltMaghrebV1 |flux= Ncbi |étape= Checkpoint |type= RBID |clé= pubmed:26678662 |texte= Pathophysiological effects of Cerastes cerastes and Vipera lebetina venoms: Immunoneutralization using anti-native and anti-(60)Co irradiated venoms. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:26678662" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a CobaltMaghrebV1
![]() | This area was generated with Dilib version V0.6.32. | ![]() |