Pathophysiological effects of Cerastes cerastes and Vipera lebetina venoms: Immunoneutralization using anti-native and anti-(60)Co irradiated venoms.
Identifieur interne : 000008 ( PubMed/Corpus ); précédent : 000007; suivant : 000009Pathophysiological effects of Cerastes cerastes and Vipera lebetina venoms: Immunoneutralization using anti-native and anti-(60)Co irradiated venoms.
Auteurs : Sabrina Boumaiza ; Habiba Oussedik-Oumehdi ; Fatima Laraba-DjebariSource :
- Biologicals : journal of the International Association of Biological Standardization [ 1095-8320 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical , antagonists & inhibitors : Viper Venoms.
- chemical , chemistry : Antivenins.
- chemical , pharmacology : Antivenins.
- chemical , toxicity : Viper Venoms.
- Animals, Cobalt Radioisotopes, Gamma Rays, Mice, Viperidae.
Abstract
Cerastes cerastes and Vipera lebetina are the most medically important vipers in Algeria. Their bite induces several pathological effects on victims of accidental envenomation. In this study we analyzed the pathogenesis induced after an experimental envenomation. Indeed, we determined, in vitro, venom enzymatic activities and we analyzed, in vivo, pathological effects induced on liver, heart, lung and skin. In addition we investigated the neutralizing potency of four experimental antivenoms elicited against native and irradiated venoms. Results revealed that V. lebetina and Cerastes cerastes venoms presented strong hemorrhagic, oedematic and necrotic activities. Histopathological study showed that both venoms induced deep damage in tissue structures leading to organ dysfunction. They also increased cellular peroxidases activities, indicating an inflammatory process that is known to amplify tissue damage. Western-blot analysis evidenced that anti-irradiated venoms recognized most components of native venoms. Antivenoms were effective in neutralizing all tested activities, with an increased protective effect obtained with anti-irradiated venoms. Anti-irradiated venoms reduced cellular peroxidases activities indicating a reduction of the inflammatory response. These results may improve our understanding of Algerian Viperidae bite pathogenesis and would encourage further studies planning to provide more proofs on the effectiveness of anti-irradiated venoms administration in the treatment of envenomation.
DOI: 10.1016/j.biologicals.2015.11.002
PubMed: 26678662
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pubmed:26678662Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Pathophysiological effects of Cerastes cerastes and Vipera lebetina venoms: Immunoneutralization using anti-native and anti-(60)Co irradiated venoms.</title><author><name sortKey="Boumaiza, Sabrina" sort="Boumaiza, Sabrina" uniqKey="Boumaiza S" first="Sabrina" last="Boumaiza">Sabrina Boumaiza</name><affiliation><nlm:affiliation>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers, Algeria.</nlm:affiliation></affiliation></author><author><name sortKey="Oussedik Oumehdi, Habiba" sort="Oussedik Oumehdi, Habiba" uniqKey="Oussedik Oumehdi H" first="Habiba" last="Oussedik-Oumehdi">Habiba Oussedik-Oumehdi</name><affiliation><nlm:affiliation>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers, Algeria.</nlm:affiliation></affiliation></author><author><name sortKey="Laraba Djebari, Fatima" sort="Laraba Djebari, Fatima" uniqKey="Laraba Djebari F" first="Fatima" last="Laraba-Djebari">Fatima Laraba-Djebari</name><affiliation><nlm:affiliation>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers, Algeria. 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Electronic address: flaraba@hotmail.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Biologicals : journal of the International Association of Biological Standardization</title><idno type="eISSN">1095-8320</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antivenins (chemistry)</term><term>Antivenins (pharmacology)</term><term>Cobalt Radioisotopes</term><term>Gamma Rays</term><term>Mice</term><term>Viper Venoms (antagonists & inhibitors)</term><term>Viper Venoms (toxicity)</term><term>Viperidae</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Viper Venoms</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antivenins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antivenins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Viper Venoms</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cobalt Radioisotopes</term><term>Gamma Rays</term><term>Mice</term><term>Viperidae</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cerastes cerastes and Vipera lebetina are the most medically important vipers in Algeria. Their bite induces several pathological effects on victims of accidental envenomation. In this study we analyzed the pathogenesis induced after an experimental envenomation. Indeed, we determined, in vitro, venom enzymatic activities and we analyzed, in vivo, pathological effects induced on liver, heart, lung and skin. In addition we investigated the neutralizing potency of four experimental antivenoms elicited against native and irradiated venoms. Results revealed that V. lebetina and Cerastes cerastes venoms presented strong hemorrhagic, oedematic and necrotic activities. Histopathological study showed that both venoms induced deep damage in tissue structures leading to organ dysfunction. They also increased cellular peroxidases activities, indicating an inflammatory process that is known to amplify tissue damage. Western-blot analysis evidenced that anti-irradiated venoms recognized most components of native venoms. Antivenoms were effective in neutralizing all tested activities, with an increased protective effect obtained with anti-irradiated venoms. Anti-irradiated venoms reduced cellular peroxidases activities indicating a reduction of the inflammatory response. These results may improve our understanding of Algerian Viperidae bite pathogenesis and would encourage further studies planning to provide more proofs on the effectiveness of anti-irradiated venoms administration in the treatment of envenomation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26678662</PMID><DateCreated><Year>2016</Year><Month>1</Month><Day>3</Day></DateCreated><DateCompleted><Year>2016</Year><Month>09</Month><Day>29</Day></DateCompleted><DateRevised><Year>2016</Year><Month>1</Month><Day>3</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1095-8320</ISSN><JournalIssue CitedMedium="Internet"><Volume>44</Volume><Issue>1</Issue><PubDate><Year>2016</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Biologicals : journal of the International Association of Biological Standardization</Title><ISOAbbreviation>Biologicals</ISOAbbreviation></Journal><ArticleTitle>Pathophysiological effects of Cerastes cerastes and Vipera lebetina venoms: Immunoneutralization using anti-native and anti-(60)Co irradiated venoms.</ArticleTitle><Pagination><MedlinePgn>1-11</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.biologicals.2015.11.002</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1045-1056(15)00135-9</ELocationID><Abstract><AbstractText>Cerastes cerastes and Vipera lebetina are the most medically important vipers in Algeria. Their bite induces several pathological effects on victims of accidental envenomation. In this study we analyzed the pathogenesis induced after an experimental envenomation. Indeed, we determined, in vitro, venom enzymatic activities and we analyzed, in vivo, pathological effects induced on liver, heart, lung and skin. In addition we investigated the neutralizing potency of four experimental antivenoms elicited against native and irradiated venoms. Results revealed that V. lebetina and Cerastes cerastes venoms presented strong hemorrhagic, oedematic and necrotic activities. Histopathological study showed that both venoms induced deep damage in tissue structures leading to organ dysfunction. They also increased cellular peroxidases activities, indicating an inflammatory process that is known to amplify tissue damage. Western-blot analysis evidenced that anti-irradiated venoms recognized most components of native venoms. Antivenoms were effective in neutralizing all tested activities, with an increased protective effect obtained with anti-irradiated venoms. Anti-irradiated venoms reduced cellular peroxidases activities indicating a reduction of the inflammatory response. These results may improve our understanding of Algerian Viperidae bite pathogenesis and would encourage further studies planning to provide more proofs on the effectiveness of anti-irradiated venoms administration in the treatment of envenomation.</AbstractText><CopyrightInformation>Copyright © 2015 The International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Boumaiza</LastName><ForeName>Sabrina</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers, Algeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Oussedik-Oumehdi</LastName><ForeName>Habiba</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers, Algeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Laraba-Djebari</LastName><ForeName>Fatima</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32, El-Alia, Bab Ezzouar, 16111, Algiers, Algeria. Electronic address: flaraba@hotmail.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>Dec</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biologicals</MedlineTA><NlmUniqueID>9004494</NlmUniqueID><ISSNLinking>1045-1056</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000997">Antivenins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003037">Cobalt Radioisotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014757">Viper Venoms</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000997" MajorTopicYN="N">Antivenins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003037" MajorTopicYN="Y">Cobalt Radioisotopes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005720" MajorTopicYN="Y">Gamma Rays</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014757" MajorTopicYN="N">Viper Venoms</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017819" MajorTopicYN="Y">Viperidae</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Gamma irradiation</Keyword><Keyword MajorTopicYN="N">Immunotherapy</Keyword><Keyword MajorTopicYN="N">Pathophysiology</Keyword><Keyword MajorTopicYN="N">Toxicity</Keyword><Keyword MajorTopicYN="N">Venom</Keyword><Keyword MajorTopicYN="N">Viper</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>3</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>11</Month><Day>9</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>12</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>12</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>9</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26678662</ArticleId><ArticleId IdType="pii">S1045-1056(15)00135-9</ArticleId><ArticleId IdType="doi">10.1016/j.biologicals.2015.11.002</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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