CobaltMaghrebV1, Main, Exploration, bibRecord, 000206

The tRNAarg Gene and engA Are Essential Genes on the 1.7-Mb pSymB Megaplasmid of Sinorhizobium meliloti and Were Translocated Together from the Chromosome in an Ancestral Strain

Identifieur interne : 000206 ( Main/Exploration ); précédent : 000205; suivant : 000207

The tRNAarg Gene and engA Are Essential Genes on the 1.7-Mb pSymB Megaplasmid of Sinorhizobium meliloti and Were Translocated Together from the Chromosome in an Ancestral Strain

Auteurs : George Dicenzo ; Branislava Milunovic ; Jiujun Cheng ; Turlough M. Finan

Source :

Journal of Bacteriology [ 0021-9193 ] ; 2013.

RBID : PMC:3553834

Abstract

Bacterial genomes with two (or more) chromosome-like replicons are known, and these appear to be particularly frequent in alphaproteobacteria. The genome of the N₂-fixing alfalfa symbiont Sinorhizobium meliloti 1021 contains a 3.7-Mb chromosome and 1.4-Mb (pSymA) and 1.7-Mb (pSymB) megaplasmids. In this study, the tRNA^arg and engA genes, located on the pSymB megaplasmid, are shown to be essential for growth. These genes could be deleted from pSymB when copies were previously integrated into the chromosome. However, in the closely related strain Sinorhizobium fredii NGR234, the tRNA^arg and engA genes are located on the chromosome, in a 69-kb region designated the engA-tRNA^arg-rmlC region. This region includes bacA, a gene that is important for intracellular survival during host-bacterium interactions for S. meliloti and the related alphaproteobacterium Brucella abortus. The engA-tRNA^arg-rmlC region lies between the kdgK and dppF2 (NGR_c24410) genes on the S. fredii chromosome. Synteny analysis showed that kdgK and dppF2 orthologues are adjacent to each other on the chromosomes of 15 sequenced strains of S. meliloti and Sinorhizobium medicae, whereas the 69-kb engA-tRNA^arg-rmlC region is present on the pSymB-equivalent megaplasmids. This and other evidence strongly suggests that the engA-tRNA^arg-rmlC region translocated from the chromosome to the progenitor of pSymB in an ancestor common to S. meliloti and S. medicae. To our knowledge, this work represents one of the first experimental demonstrations that essential genes are present on a megaplasmid.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553834

DOI: 10.1128/JB.01758-12
PubMed: 23123907
PubMed Central: 3553834

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en"><p>Bacterial genomes with two (or more) chromosome-like replicons are known, and these appear to be particularly frequent in alphaproteobacteria. The genome of the N<sub>2</sub>
-fixing alfalfa symbiont <named-content content-type="genus-species">Sinorhizobium meliloti</named-content>
 1021 contains a 3.7-Mb chromosome and 1.4-Mb (pSymA) and 1.7-Mb (pSymB) megaplasmids. In this study, the tRNA<sup>arg</sup>
 and <italic>engA</italic>
 genes, located on the pSymB megaplasmid, are shown to be essential for growth. These genes could be deleted from pSymB when copies were previously integrated into the chromosome. However, in the closely related strain <named-content content-type="genus-species">Sinorhizobium fredii</named-content>
 NGR234, the tRNA<sup>arg</sup>
 and <italic>engA</italic>
 genes are located on the chromosome, in a 69-kb region designated the <italic>engA-</italic>
tRNA<sup>arg</sup>
<italic>-rmlC</italic>
 region. This region includes <italic>bacA</italic>
, a gene that is important for intracellular survival during host-bacterium interactions for <named-content content-type="genus-species">S. meliloti</named-content>
 and the related alphaproteobacterium <named-content content-type="genus-species">Brucella abortus</named-content>
. The <italic>engA-</italic>
tRNA<sup>arg</sup>
<italic>-rmlC</italic>
 region lies between the <italic>kdgK</italic>
 and <italic>dppF2</italic>
 (NGR_c24410) genes on the <named-content content-type="genus-species">S. fredii</named-content>
 chromosome. Synteny analysis showed that <italic>kdgK</italic>
 and <italic>dppF2</italic>
 orthologues are adjacent to each other on the chromosomes of 15 sequenced strains of <named-content content-type="genus-species">S. meliloti</named-content>
 and <named-content content-type="genus-species">Sinorhizobium medicae</named-content>
, whereas the 69-kb <italic>engA-</italic>
tRNA<sup>arg</sup>
<italic>-rmlC</italic>
 region is present on the pSymB-equivalent megaplasmids. This and other evidence strongly suggests that the <italic>engA-</italic>
tRNA<sup>arg</sup>
<italic>-rmlC</italic>
 region translocated from the chromosome to the progenitor of pSymB in an ancestor common to <named-content content-type="genus-species">S. meliloti</named-content>
 and <named-content content-type="genus-species">S. medicae</named-content>
. To our knowledge, this work represents one of the first experimental demonstrations that essential genes are present on a megaplasmid.</p>
</div>
</front>
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<affiliations><list></list>
<tree><noCountry><name sortKey="Cheng, Jiujun" sort="Cheng, Jiujun" uniqKey="Cheng J" first="Jiujun" last="Cheng">Jiujun Cheng</name>
<name sortKey="Dicenzo, George" sort="Dicenzo, George" uniqKey="Dicenzo G" first="George" last="Dicenzo">George Dicenzo</name>
<name sortKey="Finan, Turlough M" sort="Finan, Turlough M" uniqKey="Finan T" first="Turlough M." last="Finan">Turlough M. Finan</name>
<name sortKey="Milunovic, Branislava" sort="Milunovic, Branislava" uniqKey="Milunovic B" first="Branislava" last="Milunovic">Branislava Milunovic</name>
</noCountry>
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The tRNAarg Gene and engA Are Essential Genes on the 1.7-Mb pSymB Megaplasmid of Sinorhizobium meliloti and Were Translocated Together from the Chromosome in an Ancestral Strain

The tRNAarg Gene and engA Are Essential Genes on the 1.7-Mb pSymB Megaplasmid of Sinorhizobium meliloti and Were Translocated Together from the Chromosome in an Ancestral Strain

Source :

Abstract

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