The tRNAarg Gene and engA Are Essential Genes on the 1.7-Mb pSymB Megaplasmid of Sinorhizobium meliloti and Were Translocated Together from the Chromosome in an Ancestral Strain
Identifieur interne : 000234 ( Ncbi/Checkpoint ); précédent : 000233; suivant : 000235The tRNAarg Gene and engA Are Essential Genes on the 1.7-Mb pSymB Megaplasmid of Sinorhizobium meliloti and Were Translocated Together from the Chromosome in an Ancestral Strain
Auteurs : George Dicenzo ; Branislava Milunovic ; Jiujun Cheng ; Turlough M. FinanSource :
- Journal of Bacteriology [ 0021-9193 ] ; 2013.
Abstract
Bacterial genomes with two (or more) chromosome-like replicons are known, and these appear to be particularly frequent in alphaproteobacteria. The genome of the N2-fixing alfalfa symbiont
Url:
DOI: 10.1128/JB.01758-12
PubMed: 23123907
PubMed Central: 3553834
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The tRNA<sup>arg</sup>
Gene and <italic>engA</italic>
Are Essential Genes on the 1.7-Mb pSymB Megaplasmid of <named-content content-type="genus-species">Sinorhizobium meliloti</named-content>
and Were Translocated Together from the Chromosome in an Ancestral Strain</title>
<author><name sortKey="Dicenzo, George" sort="Dicenzo, George" uniqKey="Dicenzo G" first="George" last="Dicenzo">George Dicenzo</name>
</author>
<author><name sortKey="Milunovic, Branislava" sort="Milunovic, Branislava" uniqKey="Milunovic B" first="Branislava" last="Milunovic">Branislava Milunovic</name>
</author>
<author><name sortKey="Cheng, Jiujun" sort="Cheng, Jiujun" uniqKey="Cheng J" first="Jiujun" last="Cheng">Jiujun Cheng</name>
</author>
<author><name sortKey="Finan, Turlough M" sort="Finan, Turlough M" uniqKey="Finan T" first="Turlough M." last="Finan">Turlough M. Finan</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The tRNA<sup>arg</sup>
Gene and <italic>engA</italic>
Are Essential Genes on the 1.7-Mb pSymB Megaplasmid of <named-content content-type="genus-species">Sinorhizobium meliloti</named-content>
and Were Translocated Together from the Chromosome in an Ancestral Strain</title>
<author><name sortKey="Dicenzo, George" sort="Dicenzo, George" uniqKey="Dicenzo G" first="George" last="Dicenzo">George Dicenzo</name>
</author>
<author><name sortKey="Milunovic, Branislava" sort="Milunovic, Branislava" uniqKey="Milunovic B" first="Branislava" last="Milunovic">Branislava Milunovic</name>
</author>
<author><name sortKey="Cheng, Jiujun" sort="Cheng, Jiujun" uniqKey="Cheng J" first="Jiujun" last="Cheng">Jiujun Cheng</name>
</author>
<author><name sortKey="Finan, Turlough M" sort="Finan, Turlough M" uniqKey="Finan T" first="Turlough M." last="Finan">Turlough M. Finan</name>
</author>
</analytic>
<series><title level="j">Journal of Bacteriology</title>
<idno type="ISSN">0021-9193</idno>
<idno type="eISSN">1098-5530</idno>
<imprint><date when="2013">2013</date>
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<front><div type="abstract" xml:lang="en"><p>Bacterial genomes with two (or more) chromosome-like replicons are known, and these appear to be particularly frequent in alphaproteobacteria. The genome of the N<sub>2</sub>
-fixing alfalfa symbiont <named-content content-type="genus-species">Sinorhizobium meliloti</named-content>
1021 contains a 3.7-Mb chromosome and 1.4-Mb (pSymA) and 1.7-Mb (pSymB) megaplasmids. In this study, the tRNA<sup>arg</sup>
and <italic>engA</italic>
genes, located on the pSymB megaplasmid, are shown to be essential for growth. These genes could be deleted from pSymB when copies were previously integrated into the chromosome. However, in the closely related strain <named-content content-type="genus-species">Sinorhizobium fredii</named-content>
NGR234, the tRNA<sup>arg</sup>
and <italic>engA</italic>
genes are located on the chromosome, in a 69-kb region designated the <italic>engA-</italic>
tRNA<sup>arg</sup>
<italic>-rmlC</italic>
region. This region includes <italic>bacA</italic>
, a gene that is important for intracellular survival during host-bacterium interactions for <named-content content-type="genus-species">S. meliloti</named-content>
and the related alphaproteobacterium <named-content content-type="genus-species">Brucella abortus</named-content>
. The <italic>engA-</italic>
tRNA<sup>arg</sup>
<italic>-rmlC</italic>
region lies between the <italic>kdgK</italic>
and <italic>dppF2</italic>
(NGR_c24410) genes on the <named-content content-type="genus-species">S. fredii</named-content>
chromosome. Synteny analysis showed that <italic>kdgK</italic>
and <italic>dppF2</italic>
orthologues are adjacent to each other on the chromosomes of 15 sequenced strains of <named-content content-type="genus-species">S. meliloti</named-content>
and <named-content content-type="genus-species">Sinorhizobium medicae</named-content>
, whereas the 69-kb <italic>engA-</italic>
tRNA<sup>arg</sup>
<italic>-rmlC</italic>
region is present on the pSymB-equivalent megaplasmids. This and other evidence strongly suggests that the <italic>engA-</italic>
tRNA<sup>arg</sup>
<italic>-rmlC</italic>
region translocated from the chromosome to the progenitor of pSymB in an ancestor common to <named-content content-type="genus-species">S. meliloti</named-content>
and <named-content content-type="genus-species">S. medicae</named-content>
. To our knowledge, this work represents one of the first experimental demonstrations that essential genes are present on a megaplasmid.</p>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Cheng, Jiujun" sort="Cheng, Jiujun" uniqKey="Cheng J" first="Jiujun" last="Cheng">Jiujun Cheng</name>
<name sortKey="Dicenzo, George" sort="Dicenzo, George" uniqKey="Dicenzo G" first="George" last="Dicenzo">George Dicenzo</name>
<name sortKey="Finan, Turlough M" sort="Finan, Turlough M" uniqKey="Finan T" first="Turlough M." last="Finan">Turlough M. Finan</name>
<name sortKey="Milunovic, Branislava" sort="Milunovic, Branislava" uniqKey="Milunovic B" first="Branislava" last="Milunovic">Branislava Milunovic</name>
</noCountry>
</tree>
</affiliations>
</record>
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