JENUFA GEN. NOV.: A NEW GENUS OF COCCOID GREEN ALGAE (CHLOROPHYCEAE, INCERTAE SEDIS) PREVIOUSLY RECORDED BY ENVIRONMENTAL SEQUENCING
Identifieur interne : 000698 ( Istex/Corpus ); précédent : 000697; suivant : 000699JENUFA GEN. NOV.: A NEW GENUS OF COCCOID GREEN ALGAE (CHLOROPHYCEAE, INCERTAE SEDIS) PREVIOUSLY RECORDED BY ENVIRONMENTAL SEQUENCING
Auteurs : Yvonne N Mcová ; Marek Eliáš ; Pavel Škaloud ; Ladislav Hoda ; Ji NeustupaSource :
- Journal of Phycology [ 0022-3646 ] ; 2011-08.
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- KwdEn :
Abstract
The diversity of eukaryotic microorganisms is far from fully described, as indicated by the vast number of unassigned genotypes retrieved by environmental sequencing or metagenomics. We isolated several strains of unicellular green algae from algal biofilms growing on tree bark in a Southeast Asian tropical rainforest and determined them to be relatives of an unidentified lineage of environmental 18S rDNA sequences, thus uncovering its cellular identity. Light, confocal, and electron microscope observations and sequencing the 18S rRNA gene revealed that the strains represent two different species within an apparently new genus, described here as Jenufa gen. nov. Both species formed minute coccoid cells with an irregular globular outline, a smooth cell wall, and a single parietal chloroplast without a pyrenoid. The two species, described herein as J. perforata and J. minuta, differed in chloroplast morphology and cell wall structure. Phylogenetic analyses of 18S rRNA gene sequences showed a firm relationship between the two species and placed the Jenufa lineage in an unresolved position within the CS clade (Chlamydomonadales + Sphaeropleales) of the class Chlorophyceae, although possible affinities to the genus Golenkinia were suggested both by maximum‐likelihood (ML) and Bayesian methods. Furthermore, two almost identical environmental 18S rDNA sequences from an endolithic microbial community occurring in dolomite rock in the central Alps turned out to be specifically related to, yet apparently distinct from, the sequence of J. minuta, indicating the existence of an undescribed Jenufa species occurring in the temperate zone.
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DOI: 10.1111/j.1529-8817.2011.01009.x
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We isolated several strains of unicellular green algae from algal biofilms growing on tree bark in a Southeast Asian tropical rainforest and determined them to be relatives of an unidentified lineage of environmental 18S rDNA sequences, thus uncovering its cellular identity. Light, confocal, and electron microscope observations and sequencing the 18S rRNA gene revealed that the strains represent two different species within an apparently new genus, described here as Jenufa gen. nov. Both species formed minute coccoid cells with an irregular globular outline, a smooth cell wall, and a single parietal chloroplast without a pyrenoid. The two species, described herein as J. perforata and J. minuta, differed in chloroplast morphology and cell wall structure. Phylogenetic analyses of 18S rRNA gene sequences showed a firm relationship between the two species and placed the Jenufa lineage in an unresolved position within the CS clade (Chlamydomonadales + Sphaeropleales) of the class Chlorophyceae, although possible affinities to the genus Golenkinia were suggested both by maximum‐likelihood (ML) and Bayesian methods. Furthermore, two almost identical environmental 18S rDNA sequences from an endolithic microbial community occurring in dolomite rock in the central Alps turned out to be specifically related to, yet apparently distinct from, the sequence of J. minuta, indicating the existence of an undescribed Jenufa species occurring in the temperate zone.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Yvonne Němcová</name><affiliations><json:string>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic</json:string></affiliations></json:item><json:item><name>Marek Eliáš</name><affiliations><json:string>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech RepublicDepartment of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Slezská Ostrava, Czech Republic</json:string></affiliations></json:item><json:item><name>Pavel Škaloud</name><affiliations><json:string>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic</json:string></affiliations></json:item><json:item><name>Ladislav Hodač</name><affiliations><json:string>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic</json:string></affiliations></json:item><json:item><name>Jiří Neustupa</name><affiliations><json:string>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>18S rRNA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Chlorophyceae</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Chlorophyta</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Jenufa</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>phylogeny</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>taxonomy</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ultrastructure</value></json:item></subject><articleId><json:string>JPY1009</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>The diversity of eukaryotic microorganisms is far from fully described, as indicated by the vast number of unassigned genotypes retrieved by environmental sequencing or metagenomics. We isolated several strains of unicellular green algae from algal biofilms growing on tree bark in a Southeast Asian tropical rainforest and determined them to be relatives of an unidentified lineage of environmental 18S rDNA sequences, thus uncovering its cellular identity. Light, confocal, and electron microscope observations and sequencing the 18S rRNA gene revealed that the strains represent two different species within an apparently new genus, described here as Jenufa gen. nov. Both species formed minute coccoid cells with an irregular globular outline, a smooth cell wall, and a single parietal chloroplast without a pyrenoid. The two species, described herein as J. perforata and J. minuta, differed in chloroplast morphology and cell wall structure. Phylogenetic analyses of 18S rRNA gene sequences showed a firm relationship between the two species and placed the Jenufa lineage in an unresolved position within the CS clade (Chlamydomonadales + Sphaeropleales) of the class Chlorophyceae, although possible affinities to the genus Golenkinia were suggested both by maximum‐likelihood (ML) and Bayesian methods. Furthermore, two almost identical environmental 18S rDNA sequences from an endolithic microbial community occurring in dolomite rock in the central Alps turned out to be specifically related to, yet apparently distinct from, the sequence of J. minuta, indicating the existence of an undescribed Jenufa species occurring in the temperate zone.</abstract><qualityIndicators><score>7.868</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595.276 x 782.362 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>7</keywordCount><abstractCharCount>1652</abstractCharCount><pdfWordCount>6575</pdfWordCount><pdfCharCount>42882</pdfCharCount><pdfPageCount>11</pdfPageCount><abstractWordCount>239</abstractWordCount></qualityIndicators><title>JENUFA GEN. 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NOV.: A NEW GENUS OF COCCOID GREEN ALGAE (CHLOROPHYCEAE, INCERTAE SEDIS) PREVIOUSLY RECORDED BY ENVIRONMENTAL SEQUENCING</title><author xml:id="author-1"><persName><forename type="first">Yvonne</forename><surname>Němcová</surname></persName><affiliation>Author for correspondence: e‐mail ynemcova@natur.cuni.cz.</affiliation><affiliation>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic</affiliation></author><author xml:id="author-2"><persName><forename type="first">Marek</forename><surname>Eliáš</surname></persName><affiliation>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech RepublicDepartment of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Slezská Ostrava, Czech Republic</affiliation></author><author xml:id="author-3"><persName><forename type="first">Pavel</forename><surname>Škaloud</surname></persName><affiliation>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic</affiliation></author><author xml:id="author-4"><persName><forename type="first">Ladislav</forename><surname>Hodač</surname></persName><note type="biography">Present address: Experimental Phycology and Culture Collection of Algae, Department of the Albrecht‐von‐Haller‐Institute for Plant Sciences at the University of, Göttingen, Untere Karspüle 2, D‐37073 Göttingen, Germany.</note><affiliation>Present address: Experimental Phycology and Culture Collection of Algae, Department of the Albrecht‐von‐Haller‐Institute for Plant Sciences at the University of, Göttingen, Untere Karspüle 2, D‐37073 Göttingen, Germany.</affiliation><affiliation>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic</affiliation></author><author xml:id="author-5"><persName><forename type="first">Jiří</forename><surname>Neustupa</surname></persName><affiliation>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic</affiliation></author></analytic><monogr><title level="j">Journal of Phycology</title><idno type="pISSN">0022-3646</idno><idno type="eISSN">1529-8817</idno><idno type="DOI">10.1111/(ISSN)1529-8817</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2011-08"></date><biblScope unit="volume">47</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="928">928</biblScope><biblScope unit="page" to="938">938</biblScope></imprint></monogr><idno type="istex">C276B93CECE58993BFB28AE0DA160310FC66F08F</idno><idno type="DOI">10.1111/j.1529-8817.2011.01009.x</idno><idno type="ArticleID">JPY1009</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2011</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The diversity of eukaryotic microorganisms is far from fully described, as indicated by the vast number of unassigned genotypes retrieved by environmental sequencing or metagenomics. We isolated several strains of unicellular green algae from algal biofilms growing on tree bark in a Southeast Asian tropical rainforest and determined them to be relatives of an unidentified lineage of environmental 18S rDNA sequences, thus uncovering its cellular identity. Light, confocal, and electron microscope observations and sequencing the 18S rRNA gene revealed that the strains represent two different species within an apparently new genus, described here as Jenufa gen. nov. Both species formed minute coccoid cells with an irregular globular outline, a smooth cell wall, and a single parietal chloroplast without a pyrenoid. The two species, described herein as J. perforata and J. minuta, differed in chloroplast morphology and cell wall structure. Phylogenetic analyses of 18S rRNA gene sequences showed a firm relationship between the two species and placed the Jenufa lineage in an unresolved position within the CS clade (Chlamydomonadales + Sphaeropleales) of the class Chlorophyceae, although possible affinities to the genus Golenkinia were suggested both by maximum‐likelihood (ML) and Bayesian methods. Furthermore, two almost identical environmental 18S rDNA sequences from an endolithic microbial community occurring in dolomite rock in the central Alps turned out to be specifically related to, yet apparently distinct from, the sequence of J. minuta, indicating the existence of an undescribed Jenufa species occurring in the temperate zone.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>18S rRNA</term></item><item><term>Chlorophyceae</term></item><item><term>Chlorophyta</term></item><item><term>Jenufa</term></item><item><term>phylogeny</term></item><item><term>taxonomy</term></item><item><term>ultrastructure</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2011-08">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/C276B93CECE58993BFB28AE0DA160310FC66F08F/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1529-8817</doi><issn type="print">0022-3646</issn><issn type="electronic">1529-8817</issn><idGroup><id type="product" value="JPY"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF PHYCOLOGY">Journal of Phycology</title></titleGroup></publicationMeta><publicationMeta level="part" position="08104"><doi origin="wiley">10.1111/jpy.2011.47.issue-4</doi><numberingGroup><numbering type="journalVolume" number="47">47</numbering><numbering type="journalIssue" number="4">4</numbering></numberingGroup><coverDate startDate="2011-08">August 2011</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="21" status="forIssue"><doi origin="wiley">10.1111/j.1529-8817.2011.01009.x</doi><idGroup><id type="unit" value="JPY1009"></id></idGroup><countGroup><count type="pageTotal" number="11"></count></countGroup><titleGroup><title type="tocHeading1">Phylogenetics and Taxonomy</title></titleGroup><copyright>© 2011 Phycological Society of America</copyright><eventGroup><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.5.2 mode:FullText" date="2011-07-25"></event><event type="publishedOnlineEarlyUnpaginated" date="2011-06-15"></event><event type="firstOnline" date="2011-06-15"></event><event type="publishedOnlineFinalForm" date="2011-07-25"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-02-01"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="928">928</numbering><numbering type="pageLast" number="938">938</numbering></numberingGroup><linkGroup><link type="toTypesetVersion" href="file:JPY.JPY1009.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="0"></count></countGroup><titleGroup><title type="main"><i>JENUFA</i> GEN. NOV.: A NEW GENUS OF COCCOID GREEN ALGAE (CHLOROPHYCEAE, INCERTAE SEDIS) PREVIOUSLY RECORDED BY ENVIRONMENTAL SEQUENCING<link href="#fn1"><sup>1</sup></link></title><title type="tocForm"><i>JENUFA</i> GEN. NOV.: A NEW GENUS OF COCCOID GREEN ALGAE (CHLOROPHYCEAE, INCERTAE SEDIS) PREVIOUSLY RECORDED BY ENVIRONMENTAL SEQUENCING</title><title type="shortAuthors">YVONNE NĚMcovÁ ET AL.</title><title type="short"><i>JENUFA</i> GEN. NOV.: A NEW GENUS OF COCCOID GREEN ALGAE</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1" noteRef="#fn2"><personName><givenNames>Yvonne</givenNames><familyName>Němcová</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>Marek</givenNames><familyName>Eliáš</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a3"><personName><givenNames>Pavel</givenNames><familyName>Škaloud</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a3" noteRef="#fn3"><personName><givenNames>Ladislav</givenNames><familyName>Hodač</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a3"><personName><givenNames>Jiří</givenNames><familyName>Neustupa</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="CZ"><unparsedAffiliation>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="CZ"><unparsedAffiliation> Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic
Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Slezská Ostrava, Czech Republic</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="CZ"><unparsedAffiliation>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">18S rRNA</keyword><keyword xml:id="k2">Chlorophyceae</keyword><keyword xml:id="k3">Chlorophyta</keyword><keyword xml:id="k4"><i>Jenufa</i></keyword><keyword xml:id="k5">phylogeny</keyword><keyword xml:id="k6">taxonomy</keyword><keyword xml:id="k7">ultrastructure</keyword></keywordGroup><supportingInformation><p><b>Table S1.</b> List of algal strains characterized in this study.</p><supportingInfoItem><mediaResource alt="supporting info item" href="urn-x:wiley:00223646:media:jpy1009:JPY_1009_sm_TableS1"></mediaResource><caption>Supporting info item</caption></supportingInfoItem></supportingInformation><abstractGroup><abstract type="main" xml:lang="en"><p>The diversity of eukaryotic microorganisms is far from fully described, as indicated by the vast number of unassigned genotypes retrieved by environmental sequencing or metagenomics. We isolated several strains of unicellular green algae from algal biofilms growing on tree bark in a Southeast Asian tropical rainforest and determined them to be relatives of an unidentified lineage of environmental 18S rDNA sequences, thus uncovering its cellular identity. Light, confocal, and electron microscope observations and sequencing the 18S rRNA gene revealed that the strains represent two different species within an apparently new genus, described here as <i>Jenufa</i> gen. nov. Both species formed minute coccoid cells with an irregular globular outline, a smooth cell wall, and a single parietal chloroplast without a pyrenoid. The two species, described herein as <i>J. perforata</i> and <i>J. minuta</i>, differed in chloroplast morphology and cell wall structure. Phylogenetic analyses of 18S rRNA gene sequences showed a firm relationship between the two species and placed the <i>Jenufa</i> lineage in an unresolved position within the CS clade (Chlamydomonadales + Sphaeropleales) of the class Chlorophyceae, although possible affinities to the genus <i>Golenkinia</i> were suggested both by maximum‐likelihood (ML) and Bayesian methods. Furthermore, two almost identical environmental 18S rDNA sequences from an endolithic microbial community occurring in dolomite rock in the central Alps turned out to be specifically related to, yet apparently distinct from, the sequence of <i>J. minuta</i>, indicating the existence of an undescribed <i>Jenufa</i> species occurring in the temperate zone.</p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="fn1"><p> Received 18 October 2010. Accepted 16 February 2011.</p></note><note xml:id="fn2"><p> Author for correspondence: e‐mail <email>ynemcova@natur.cuni.cz</email>.</p></note><note xml:id="fn3"><p> Present address: Experimental Phycology and Culture Collection of Algae, Department of the Albrecht‐von‐Haller‐Institute for Plant Sciences at the University of, Göttingen, Untere Karspüle 2, D‐37073 Göttingen, Germany.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>JENUFA GEN. NOV.: A NEW GENUS OF COCCOID GREEN ALGAE (CHLOROPHYCEAE, INCERTAE SEDIS) PREVIOUSLY RECORDED BY ENVIRONMENTAL SEQUENCING</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>JENUFA GEN. NOV.: A NEW GENUS OF COCCOID GREEN ALGAE</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>JENUFA GEN. NOV.: A NEW GENUS OF COCCOID GREEN ALGAE (CHLOROPHYCEAE, INCERTAE SEDIS) PREVIOUSLY RECORDED BY ENVIRONMENTAL SEQUENCING1</title></titleInfo><name type="personal"><namePart type="given">Yvonne</namePart><namePart type="family">Němcová</namePart><affiliation>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic</affiliation><description>Author for correspondence: e‐mail ynemcova@natur.cuni.cz.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marek</namePart><namePart type="family">Eliáš</namePart><affiliation>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech RepublicDepartment of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Slezská Ostrava, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Pavel</namePart><namePart type="family">Škaloud</namePart><affiliation>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ladislav</namePart><namePart type="family">Hodač</namePart><affiliation>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic</affiliation><description>Present address: Experimental Phycology and Culture Collection of Algae, Department of the Albrecht‐von‐Haller‐Institute for Plant Sciences at the University of, Göttingen, Untere Karspüle 2, D‐37073 Göttingen, Germany.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jiří</namePart><namePart type="family">Neustupa</namePart><affiliation>Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague 2, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2011-08</dateIssued><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">4</extent></physicalDescription><abstract lang="en">The diversity of eukaryotic microorganisms is far from fully described, as indicated by the vast number of unassigned genotypes retrieved by environmental sequencing or metagenomics. We isolated several strains of unicellular green algae from algal biofilms growing on tree bark in a Southeast Asian tropical rainforest and determined them to be relatives of an unidentified lineage of environmental 18S rDNA sequences, thus uncovering its cellular identity. Light, confocal, and electron microscope observations and sequencing the 18S rRNA gene revealed that the strains represent two different species within an apparently new genus, described here as Jenufa gen. nov. Both species formed minute coccoid cells with an irregular globular outline, a smooth cell wall, and a single parietal chloroplast without a pyrenoid. The two species, described herein as J. perforata and J. minuta, differed in chloroplast morphology and cell wall structure. Phylogenetic analyses of 18S rRNA gene sequences showed a firm relationship between the two species and placed the Jenufa lineage in an unresolved position within the CS clade (Chlamydomonadales + Sphaeropleales) of the class Chlorophyceae, although possible affinities to the genus Golenkinia were suggested both by maximum‐likelihood (ML) and Bayesian methods. Furthermore, two almost identical environmental 18S rDNA sequences from an endolithic microbial community occurring in dolomite rock in the central Alps turned out to be specifically related to, yet apparently distinct from, the sequence of J. minuta, indicating the existence of an undescribed Jenufa species occurring in the temperate zone.</abstract><note type="content">*Received 18 October 2010. Accepted 16 February 2011.</note><subject lang="en"><genre>keywords</genre><topic>18S rRNA</topic><topic>Chlorophyceae</topic><topic>Chlorophyta</topic><topic>Jenufa</topic><topic>phylogeny</topic><topic>taxonomy</topic><topic>ultrastructure</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Phycology</title></titleInfo><genre type="journal">journal</genre><note type="content"> Table S1. List of algal strains characterized in this study.Supporting Info Item: Supporting info item - </note><identifier type="ISSN">0022-3646</identifier><identifier type="eISSN">1529-8817</identifier><identifier type="DOI">10.1111/(ISSN)1529-8817</identifier><identifier type="PublisherID">JPY</identifier><part><date>2011</date><detail type="volume"><caption>vol.</caption><number>47</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>928</start><end>938</end><total>11</total></extent></part></relatedItem><identifier type="istex">C276B93CECE58993BFB28AE0DA160310FC66F08F</identifier><identifier type="DOI">10.1111/j.1529-8817.2011.01009.x</identifier><identifier type="ArticleID">JPY1009</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2011 Phycological Society of America</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><enrichments><json:item><type>multicat</type><uri>https://api.istex.fr/document/C276B93CECE58993BFB28AE0DA160310FC66F08F/enrichments/multicat</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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