Three-dimensional axon morphologies of individual layer 5 neurons indicate cell type-specific intracortical pathways for whisker motion and touch
Identifieur interne : 001932 ( Ncbi/Merge ); précédent : 001931; suivant : 001933Three-dimensional axon morphologies of individual layer 5 neurons indicate cell type-specific intracortical pathways for whisker motion and touch
Auteurs : Marcel Oberlaender [États-Unis] ; Zimbo S. R. M. Boudewijns [Pays-Bas] ; Tatjana Kleele [Allemagne] ; Huibert D. Mansvelder [Pays-Bas] ; Bert Sakmann [États-Unis] ; Christiaan P. J. De Kock [Pays-Bas]Source :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2011.
Abstract
The cortical output layer 5 contains two excitatory cell types, slender- and thick-tufted neurons. In rat vibrissal cortex, slender-tufted neurons carry motion and phase information during active whisking, but remain inactive after passive whisker touch. In contrast, thick-tufted neurons reliably increase spiking preferably after passive touch. By reconstructing the 3D patterns of intracortical axon projections from individual slender- and thick-tufted neurons, filled in vivo with biocytin, we were able to identify cell type-specific intracortical circuits that may encode whisker motion and touch. Individual slender-tufted neurons showed elaborate and dense innervation of supragranular layers of large portions of the vibrissal area (total length, 86.8 ± 5.5 mm). During active whisking, these long-range projections may modulate and phase-lock the membrane potential of dendrites in layers 2 and 3 to the whisking cycle. Thick-tufted neurons with soma locations intermingling with those of slender-tufted ones display less dense intracortical axon projections (total length, 31.6 ± 14.3 mm) that are primarily confined to infragranular layers. Based on anatomical reconstructions and previous measurements of spiking, we put forward the hypothesis that thick-tufted neurons in rat vibrissal cortex receive input of whisker motion from slender-tufted neurons onto their apical tuft dendrites and input of whisker touch from thalamic neurons onto their basal dendrites. During tactile-driven behavior, such as object location, near-coincident input from these two pathways may result in increased spiking activity of thick-tufted neurons and thus enhanced signaling to their subcortical targets.
Url:
DOI: 10.1073/pnas.1100647108
PubMed: 21368112
PubMed Central: 3053980
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 001542
- to stream Pmc, to step Curation: 001542
- to stream Pmc, to step Checkpoint: 001939
Links to Exploration step
PMC:3053980Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Three-dimensional axon morphologies of individual layer 5 neurons indicate cell type-specific intracortical pathways for whisker motion and touch</title>
<author><name sortKey="Oberlaender, Marcel" sort="Oberlaender, Marcel" uniqKey="Oberlaender M" first="Marcel" last="Oberlaender">Marcel Oberlaender</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Digital Neuroanatomy,<institution>Max Planck Florida Institute</institution>
, Jupiter, FL 33458-2906;</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Floride</region>
</placeName>
<wicri:cityArea>, Jupiter</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Boudewijns, Zimbo S R M" sort="Boudewijns, Zimbo S R M" uniqKey="Boudewijns Z" first="Zimbo S. R. M." last="Boudewijns">Zimbo S. R. M. Boudewijns</name>
<affiliation wicri:level="1"><nlm:aff wicri:cut="; and" id="aff2">Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam,<institution>Vrije Universiteit Amsterdam</institution>
, NL-1087 HV, Amsterdam,<country>The Netherlands</country>
</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kleele, Tatjana" sort="Kleele, Tatjana" uniqKey="Kleele T" first="Tatjana" last="Kleele">Tatjana Kleele</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">Institute of Neuroscience,<institution>Technical University Munich</institution>
, D-80802 Munich,<country>Germany</country>
</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mansvelder, Huibert D" sort="Mansvelder, Huibert D" uniqKey="Mansvelder H" first="Huibert D." last="Mansvelder">Huibert D. Mansvelder</name>
<affiliation wicri:level="1"><nlm:aff wicri:cut="; and" id="aff2">Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam,<institution>Vrije Universiteit Amsterdam</institution>
, NL-1087 HV, Amsterdam,<country>The Netherlands</country>
</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Sakmann, Bert" sort="Sakmann, Bert" uniqKey="Sakmann B" first="Bert" last="Sakmann">Bert Sakmann</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Digital Neuroanatomy,<institution>Max Planck Florida Institute</institution>
, Jupiter, FL 33458-2906;</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Floride</region>
</placeName>
<wicri:cityArea>, Jupiter</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="De Kock, Christiaan P J" sort="De Kock, Christiaan P J" uniqKey="De Kock C" first="Christiaan P. J." last="De Kock">Christiaan P. J. De Kock</name>
<affiliation wicri:level="1"><nlm:aff wicri:cut="; and" id="aff2">Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam,<institution>Vrije Universiteit Amsterdam</institution>
, NL-1087 HV, Amsterdam,<country>The Netherlands</country>
</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">21368112</idno>
<idno type="pmc">3053980</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3053980</idno>
<idno type="RBID">PMC:3053980</idno>
<idno type="doi">10.1073/pnas.1100647108</idno>
<date when="2011">2011</date>
<idno type="wicri:Area/Pmc/Corpus">001542</idno>
<idno type="wicri:Area/Pmc/Curation">001542</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001939</idno>
<idno type="wicri:Area/Ncbi/Merge">001932</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Three-dimensional axon morphologies of individual layer 5 neurons indicate cell type-specific intracortical pathways for whisker motion and touch</title>
<author><name sortKey="Oberlaender, Marcel" sort="Oberlaender, Marcel" uniqKey="Oberlaender M" first="Marcel" last="Oberlaender">Marcel Oberlaender</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Digital Neuroanatomy,<institution>Max Planck Florida Institute</institution>
, Jupiter, FL 33458-2906;</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Floride</region>
</placeName>
<wicri:cityArea>, Jupiter</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Boudewijns, Zimbo S R M" sort="Boudewijns, Zimbo S R M" uniqKey="Boudewijns Z" first="Zimbo S. R. M." last="Boudewijns">Zimbo S. R. M. Boudewijns</name>
<affiliation wicri:level="1"><nlm:aff wicri:cut="; and" id="aff2">Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam,<institution>Vrije Universiteit Amsterdam</institution>
, NL-1087 HV, Amsterdam,<country>The Netherlands</country>
</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kleele, Tatjana" sort="Kleele, Tatjana" uniqKey="Kleele T" first="Tatjana" last="Kleele">Tatjana Kleele</name>
<affiliation wicri:level="1"><nlm:aff id="aff3">Institute of Neuroscience,<institution>Technical University Munich</institution>
, D-80802 Munich,<country>Germany</country>
</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mansvelder, Huibert D" sort="Mansvelder, Huibert D" uniqKey="Mansvelder H" first="Huibert D." last="Mansvelder">Huibert D. Mansvelder</name>
<affiliation wicri:level="1"><nlm:aff wicri:cut="; and" id="aff2">Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam,<institution>Vrije Universiteit Amsterdam</institution>
, NL-1087 HV, Amsterdam,<country>The Netherlands</country>
</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Sakmann, Bert" sort="Sakmann, Bert" uniqKey="Sakmann B" first="Bert" last="Sakmann">Bert Sakmann</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Digital Neuroanatomy,<institution>Max Planck Florida Institute</institution>
, Jupiter, FL 33458-2906;</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Floride</region>
</placeName>
<wicri:cityArea>, Jupiter</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="De Kock, Christiaan P J" sort="De Kock, Christiaan P J" uniqKey="De Kock C" first="Christiaan P. J." last="De Kock">Christiaan P. J. De Kock</name>
<affiliation wicri:level="1"><nlm:aff wicri:cut="; and" id="aff2">Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam,<institution>Vrije Universiteit Amsterdam</institution>
, NL-1087 HV, Amsterdam,<country>The Netherlands</country>
</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title>
<idno type="ISSN">0027-8424</idno>
<idno type="eISSN">1091-6490</idno>
<imprint><date when="2011">2011</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The cortical output layer 5 contains two excitatory cell types, slender- and thick-tufted neurons. In rat vibrissal cortex, slender-tufted neurons carry motion and phase information during active whisking, but remain inactive after passive whisker touch. In contrast, thick-tufted neurons reliably increase spiking preferably after passive touch. By reconstructing the 3D patterns of intracortical axon projections from individual slender- and thick-tufted neurons, filled in vivo with biocytin, we were able to identify cell type-specific intracortical circuits that may encode whisker motion and touch. Individual slender-tufted neurons showed elaborate and dense innervation of supragranular layers of large portions of the vibrissal area (total length, 86.8 ± 5.5 mm). During active whisking, these long-range projections may modulate and phase-lock the membrane potential of dendrites in layers 2 and 3 to the whisking cycle. Thick-tufted neurons with soma locations intermingling with those of slender-tufted ones display less dense intracortical axon projections (total length, 31.6 ± 14.3 mm) that are primarily confined to infragranular layers. Based on anatomical reconstructions and previous measurements of spiking, we put forward the hypothesis that thick-tufted neurons in rat vibrissal cortex receive input of whisker motion from slender-tufted neurons onto their apical tuft dendrites and input of whisker touch from thalamic neurons onto their basal dendrites. During tactile-driven behavior, such as object location, near-coincident input from these two pathways may result in increased spiking activity of thick-tufted neurons and thus enhanced signaling to their subcortical targets.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Proc Natl Acad Sci U S A</journal-id>
<journal-id journal-id-type="hwp">pnas</journal-id>
<journal-id journal-id-type="pmc">pnas</journal-id>
<journal-id journal-id-type="publisher-id">PNAS</journal-id>
<journal-title-group><journal-title>Proceedings of the National Academy of Sciences of the United States of America</journal-title>
</journal-title-group>
<issn pub-type="ppub">0027-8424</issn>
<issn pub-type="epub">1091-6490</issn>
<publisher><publisher-name>National Academy of Sciences</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">21368112</article-id>
<article-id pub-id-type="pmc">3053980</article-id>
<article-id pub-id-type="publisher-id">201100647</article-id>
<article-id pub-id-type="doi">10.1073/pnas.1100647108</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Biological Sciences</subject>
<subj-group><subject>Neuroscience</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Three-dimensional axon morphologies of individual layer 5 neurons indicate cell type-specific intracortical pathways for whisker motion and touch</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Oberlaender</surname>
<given-names>Marcel</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
<xref ref-type="corresp" rid="cor1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Boudewijns</surname>
<given-names>Zimbo S. R. M.</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kleele</surname>
<given-names>Tatjana</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>c</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Mansvelder</surname>
<given-names>Huibert D.</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sakmann</surname>
<given-names>Bert</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
<xref ref-type="corresp" rid="cor1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>de Kock</surname>
<given-names>Christiaan P. J.</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
<xref ref-type="corresp" rid="cor1"><sup>1</sup>
</xref>
</contrib>
<aff id="aff1"><sup>a</sup>
Digital Neuroanatomy,<institution>Max Planck Florida Institute</institution>
, Jupiter, FL 33458-2906;</aff>
<aff id="aff2"><sup>b</sup>
Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam,<institution>Vrije Universiteit Amsterdam</institution>
, NL-1087 HV, Amsterdam,<country>The Netherlands</country>
; and</aff>
<aff id="aff3"><sup>c</sup>
Institute of Neuroscience,<institution>Technical University Munich</institution>
, D-80802 Munich,<country>Germany</country>
</aff>
</contrib-group>
<author-notes><corresp id="cor1"><sup>1</sup>
To whom correspondence may be addressed. E-mail: <email>marcel.oberlaender@maxplanckflorida.org</email>
, <email>bert.sakmann@maxplanckflorida.org</email>
, or <email>christiaan.de.kock@cncr.vu.nl</email>
.</corresp>
<fn fn-type="edited-by"><p>Contributed by Bert Sakmann, January 19, 2011 (sent for review November 20, 2010)</p>
</fn>
<fn fn-type="con"><p>Author contributions: M.O., B.S., and C.P.J.d.K. designed research; M.O., Z.S.R.M.B., T.K., and C.P.J.d.K. performed research; M.O. designed new analytic tools; M.O., Z.S.R.M.B., H.D.M., B.S., and C.P.J.d.K. analyzed data; and M.O., Z.S.R.M.B., H.D.M., B.S., and C.P.J.d.K. wrote the paper.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><day>8</day>
<month>3</month>
<year>2011</year>
</pub-date>
<pub-date pub-type="epub"><day>22</day>
<month>2</month>
<year>2011</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>22</day>
<month>2</month>
<year>2011</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
. </pmc-comment>
<volume>108</volume>
<issue>10</issue>
<fpage>4188</fpage>
<lpage>4193</lpage>
<permissions><license license-type="open-access"><license-p>Freely available online through the PNAS open access option.</license-p>
</license>
</permissions>
<self-uri xlink:title="pdf" xlink:type="simple" xlink:href="pnas.201100647.pdf"></self-uri>
<abstract><p>The cortical output layer 5 contains two excitatory cell types, slender- and thick-tufted neurons. In rat vibrissal cortex, slender-tufted neurons carry motion and phase information during active whisking, but remain inactive after passive whisker touch. In contrast, thick-tufted neurons reliably increase spiking preferably after passive touch. By reconstructing the 3D patterns of intracortical axon projections from individual slender- and thick-tufted neurons, filled in vivo with biocytin, we were able to identify cell type-specific intracortical circuits that may encode whisker motion and touch. Individual slender-tufted neurons showed elaborate and dense innervation of supragranular layers of large portions of the vibrissal area (total length, 86.8 ± 5.5 mm). During active whisking, these long-range projections may modulate and phase-lock the membrane potential of dendrites in layers 2 and 3 to the whisking cycle. Thick-tufted neurons with soma locations intermingling with those of slender-tufted ones display less dense intracortical axon projections (total length, 31.6 ± 14.3 mm) that are primarily confined to infragranular layers. Based on anatomical reconstructions and previous measurements of spiking, we put forward the hypothesis that thick-tufted neurons in rat vibrissal cortex receive input of whisker motion from slender-tufted neurons onto their apical tuft dendrites and input of whisker touch from thalamic neurons onto their basal dendrites. During tactile-driven behavior, such as object location, near-coincident input from these two pathways may result in increased spiking activity of thick-tufted neurons and thus enhanced signaling to their subcortical targets.</p>
</abstract>
<kwd-group><kwd>axon reconstruction</kwd>
<kwd>barrel cortex</kwd>
<kwd>dysgranular zone</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Allemagne</li>
<li>Pays-Bas</li>
<li>États-Unis</li>
</country>
<region><li>Floride</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Floride"><name sortKey="Oberlaender, Marcel" sort="Oberlaender, Marcel" uniqKey="Oberlaender M" first="Marcel" last="Oberlaender">Marcel Oberlaender</name>
</region>
<name sortKey="Sakmann, Bert" sort="Sakmann, Bert" uniqKey="Sakmann B" first="Bert" last="Sakmann">Bert Sakmann</name>
</country>
<country name="Pays-Bas"><noRegion><name sortKey="Boudewijns, Zimbo S R M" sort="Boudewijns, Zimbo S R M" uniqKey="Boudewijns Z" first="Zimbo S. R. M." last="Boudewijns">Zimbo S. R. M. Boudewijns</name>
</noRegion>
<name sortKey="De Kock, Christiaan P J" sort="De Kock, Christiaan P J" uniqKey="De Kock C" first="Christiaan P. J." last="De Kock">Christiaan P. J. De Kock</name>
<name sortKey="Mansvelder, Huibert D" sort="Mansvelder, Huibert D" uniqKey="Mansvelder H" first="Huibert D." last="Mansvelder">Huibert D. Mansvelder</name>
</country>
<country name="Allemagne"><noRegion><name sortKey="Kleele, Tatjana" sort="Kleele, Tatjana" uniqKey="Kleele T" first="Tatjana" last="Kleele">Tatjana Kleele</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Ticri/CIDE/explor/HapticV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001932 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 001932 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Ticri/CIDE |area= HapticV1 |flux= Ncbi |étape= Merge |type= RBID |clé= PMC:3053980 |texte= Three-dimensional axon morphologies of individual layer 5 neurons indicate cell type-specific intracortical pathways for whisker motion and touch }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:21368112" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \ | NlmPubMed2Wicri -a HapticV1
This area was generated with Dilib version V0.6.23. |