How Cations Can Assist DNase I in DNA Binding and Hydrolysis
Identifieur interne : 001791 ( Ncbi/Checkpoint ); précédent : 001790; suivant : 001792How Cations Can Assist DNase I in DNA Binding and Hydrolysis
Auteurs : Marc Guéroult [France] ; Daniel Picot [France] ; Joséphine Abi-Ghanem [France] ; Brigitte Hartmann [France] ; Marc Baaden [France]Source :
- PLoS Computational Biology [ 1553-734X ] ; 2010.
Abstract
DNase I requires Ca2+ and Mg2+ for hydrolyzing double-stranded DNA. However, the number and the location of DNase I ion-binding sites remain unclear, as well as the role of these counter-ions. Using molecular dynamics simulations, we show that bovine pancreatic (bp) DNase I contains four ion-binding pockets. Two of them strongly bind Ca2+ while the other two sites coordinate Mg2+. These theoretical results are strongly supported by revisiting crystallographic structures that contain bpDNase I. One Ca2+ stabilizes the functional DNase I structure. The presence of Mg2+ in close vicinity to the catalytic pocket of bpDNase I reinforces the idea of a cation-assisted hydrolytic mechanism. Importantly, Poisson-Boltzmann-type electrostatic potential calculations demonstrate that the divalent cations collectively control the electrostatic fit between bpDNase I and DNA. These results improve our understanding of the essential role of cations in the biological function of bpDNase I. The high degree of conservation of the amino acids involved in the identified cation-binding sites across DNase I and DNase I-like proteins from various species suggests that our findings generally apply to all DNase I-DNA interactions.
Url:
DOI: 10.1371/journal.pcbi.1001000
PubMed: 21124947
PubMed Central: 2987838
Affiliations:
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uniqKey="Picot D" first="Daniel" last="Picot">Daniel Picot</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>CNRS UMR 7099, Institut de Biologie Physico-Chimique, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CNRS UMR 7099, Institut de Biologie Physico-Chimique, Paris</wicri:regionArea><placeName><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Abi Ghanem, Josephine" sort="Abi Ghanem, Josephine" uniqKey="Abi Ghanem J" first="Joséphine" last="Abi-Ghanem">Joséphine Abi-Ghanem</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>INTS, INSERM UMR S665, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>INTS, INSERM UMR S665, Paris</wicri:regionArea><placeName><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Hartmann, Brigitte" sort="Hartmann, Brigitte" uniqKey="Hartmann B" 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sort="Abi Ghanem, Josephine" uniqKey="Abi Ghanem J" first="Joséphine" last="Abi-Ghanem">Joséphine Abi-Ghanem</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>INTS, INSERM UMR S665, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>INTS, INSERM UMR S665, Paris</wicri:regionArea><placeName><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Hartmann, Brigitte" sort="Hartmann, Brigitte" uniqKey="Hartmann B" first="Brigitte" last="Hartmann">Brigitte Hartmann</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>INTS, INSERM UMR S665, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>INTS, INSERM UMR S665, Paris</wicri:regionArea><placeName><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Baaden, Marc" sort="Baaden, Marc" uniqKey="Baaden M" first="Marc" last="Baaden">Marc Baaden</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>CNRS UPR 9080, Institut de Biologie Physico-Chimique, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>CNRS UPR 9080, Institut de Biologie Physico-Chimique, Paris</wicri:regionArea><placeName><settlement type="city">Paris</settlement></placeName></affiliation></author></analytic><series><title level="j">PLoS Computational Biology</title><idno type="ISSN">1553-734X</idno><idno type="eISSN">1553-7358</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>DNase I requires Ca<sup>2+</sup> and Mg<sup>2+</sup> for hydrolyzing double-stranded DNA. However, the number and the location of DNase I ion-binding sites remain unclear, as well as the role of these counter-ions. Using molecular dynamics simulations, we show that bovine pancreatic (bp) DNase I contains four ion-binding pockets. Two of them strongly bind Ca<sup>2+</sup> while the other two sites coordinate Mg<sup>2+</sup>. These theoretical results are strongly supported by revisiting crystallographic structures that contain bpDNase I. One Ca<sup>2+</sup> stabilizes the functional DNase I structure. The presence of Mg<sup>2+</sup> in close vicinity to the catalytic pocket of bpDNase I reinforces the idea of a cation-assisted hydrolytic mechanism. Importantly, Poisson-Boltzmann-type electrostatic potential calculations demonstrate that the divalent cations collectively control the electrostatic fit between bpDNase I and DNA. These results improve our understanding of the essential role of cations in the biological function of bpDNase I. The high degree of conservation of the amino acids involved in the identified cation-binding sites across DNase I and DNase I-like proteins from various species suggests that our findings generally apply to all DNase I-DNA interactions.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Laskowski, M" uniqKey="Laskowski M">M Laskowski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moore, S" uniqKey="Moore S">S Moore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Counis, Mf" uniqKey="Counis M">MF Counis</name></author><author><name sortKey="Torriglia, A" uniqKey="Torriglia A">A Torriglia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Napirei, M" uniqKey="Napirei M">M Napirei</name></author><author><name sortKey="Karsunky, H" uniqKey="Karsunky H">H Karsunky</name></author><author><name sortKey="Zevnik, B" uniqKey="Zevnik B">B Zevnik</name></author><author><name sortKey="Stephan, H" uniqKey="Stephan H">H Stephan</name></author><author><name sortKey="Mannherz, Hg" uniqKey="Mannherz H">HG Mannherz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dos Remedios, Cg" uniqKey="Dos Remedios C">CG dos Remedios</name></author><author><name sortKey="Chhabra, D" uniqKey="Chhabra D">D Chhabra</name></author><author><name sortKey="Kekic, M" uniqKey="Kekic M">M Kekic</name></author><author><name sortKey="Dedova, Iv" uniqKey="Dedova I">IV Dedova</name></author><author><name sortKey="Tsubakihara, M" uniqKey="Tsubakihara M">M Tsubakihara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Galas, Dj" uniqKey="Galas D">DJ Galas</name></author><author><name sortKey="Schmitz, A" uniqKey="Schmitz A">A Schmitz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Boyle, Ap" uniqKey="Boyle A">AP Boyle</name></author><author><name sortKey="Davis, S" uniqKey="Davis S">S Davis</name></author><author><name sortKey="Shulha, Hp" uniqKey="Shulha H">HP Shulha</name></author><author><name sortKey="Meltzer, P" uniqKey="Meltzer P">P Meltzer</name></author><author><name sortKey="Margulies, Eh" uniqKey="Margulies E">EH Margulies</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hesselberth, Jr" uniqKey="Hesselberth J">JR Hesselberth</name></author><author><name sortKey="Chen, X" uniqKey="Chen X">X Chen</name></author><author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z Zhang</name></author><author><name sortKey="Sabo, Pj" uniqKey="Sabo P">PJ Sabo</name></author><author><name sortKey="Sandstrom, R" uniqKey="Sandstrom R">R Sandstrom</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Radwan, A" uniqKey="Radwan A">A Radwan</name></author><author><name sortKey="Younis, A" uniqKey="Younis A">A Younis</name></author><author><name sortKey="Luykx, P" uniqKey="Luykx P">P Luykx</name></author><author><name sortKey="Khuri, S" uniqKey="Khuri S">S Khuri</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author><author><name sortKey="Shin, H" uniqKey="Shin H">H Shin</name></author><author><name sortKey="Song, Js" uniqKey="Song J">JS Song</name></author><author><name sortKey="Lei, Y" uniqKey="Lei Y">Y Lei</name></author><author><name sortKey="Liu, Xs" uniqKey="Liu X">XS Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ulmer, Js" uniqKey="Ulmer J">JS Ulmer</name></author><author><name sortKey="Herzka, A" uniqKey="Herzka A">A Herzka</name></author><author><name sortKey="Toy, Kj" uniqKey="Toy K">KJ Toy</name></author><author><name sortKey="Baker, Dl" uniqKey="Baker D">DL Baker</name></author><author><name sortKey="Dodge, Ah" uniqKey="Dodge A">AH Dodge</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lazarus, Ra" uniqKey="Lazarus R">RA Lazarus</name></author><author><name sortKey="Wagener, Js" uniqKey="Wagener J">JS Wagener</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Boogaard, R" uniqKey="Boogaard R">R Boogaard</name></author><author><name sortKey="De Jongste, Jc" uniqKey="De Jongste J">JC de Jongste</name></author><author><name sortKey="Merkus, Pj" uniqKey="Merkus P">PJ Merkus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martinez Valle, F" uniqKey="Martinez Valle F">F Martinez Valle</name></author><author><name sortKey="Balada, E" uniqKey="Balada E">E Balada</name></author><author><name sortKey="Ordi Ros, J" uniqKey="Ordi Ros J">J Ordi-Ros</name></author><author><name sortKey="Vilardell Tarres, M" uniqKey="Vilardell Tarres M">M Vilardell-Tarres</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gaipl, Us" uniqKey="Gaipl U">US Gaipl</name></author><author><name sortKey="Kuhn, A" uniqKey="Kuhn A">A Kuhn</name></author><author><name sortKey="Sheriff, A" uniqKey="Sheriff A">A Sheriff</name></author><author><name sortKey="Munoz, Le" uniqKey="Munoz L">LE Munoz</name></author><author><name sortKey="Franz, S" uniqKey="Franz S">S Franz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Taper, Hs" uniqKey="Taper H">HS Taper</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Melgar, E" uniqKey="Melgar E">E Melgar</name></author><author><name sortKey="Goldthwait, Da" uniqKey="Goldthwait D">DA Goldthwait</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pan, Cq" uniqKey="Pan C">CQ Pan</name></author><author><name sortKey="Ulmer, Js" uniqKey="Ulmer J">JS Ulmer</name></author><author><name sortKey="Herzka, A" uniqKey="Herzka A">A Herzka</name></author><author><name sortKey="Lazarus, Ra" uniqKey="Lazarus R">RA Lazarus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Price, Pa" uniqKey="Price P">PA Price</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Price, Pa" uniqKey="Price P">PA Price</name></author><author><name sortKey="Liu, Ty" uniqKey="Liu T">TY Liu</name></author><author><name sortKey="Stein, Wh" uniqKey="Stein W">WH Stein</name></author><author><name sortKey="Moore, S" uniqKey="Moore S">S Moore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poulos, Tl" uniqKey="Poulos T">TL Poulos</name></author><author><name sortKey="Price, Pa" uniqKey="Price P">PA Price</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tullis, R" uniqKey="Tullis R">R Tullis</name></author><author><name sortKey="Price, Pa" uniqKey="Price P">PA Price</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kabsch, W" uniqKey="Kabsch W">W Kabsch</name></author><author><name sortKey="Mannherz, Hg" uniqKey="Mannherz H">HG Mannherz</name></author><author><name sortKey="Suck, D" uniqKey="Suck D">D Suck</name></author><author><name sortKey="Pai, Ef" uniqKey="Pai E">EF Pai</name></author><author><name sortKey="Holmes, Kc" uniqKey="Holmes K">KC Holmes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Oefner, C" uniqKey="Oefner C">C Oefner</name></author><author><name sortKey="Suck, D" uniqKey="Suck D">D Suck</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chereau, D" uniqKey="Chereau D">D Chereau</name></author><author><name sortKey="Kerff, F" uniqKey="Kerff F">F Kerff</name></author><author><name sortKey="Graceffa, P" uniqKey="Graceffa P">P Graceffa</name></author><author><name sortKey="Grabarek, Z" uniqKey="Grabarek Z">Z Grabarek</name></author><author><name sortKey="Langsetmo, K" uniqKey="Langsetmo K">K Langsetmo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Cy" uniqKey="Chen C">CY Chen</name></author><author><name sortKey="Lu, Sc" uniqKey="Lu S">SC Lu</name></author><author><name sortKey="Liao, Th" uniqKey="Liao T">TH Liao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pan, Cq" uniqKey="Pan C">CQ Pan</name></author><author><name sortKey="Lazarus, Ra" uniqKey="Lazarus R">RA Lazarus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Price, Pa" uniqKey="Price P">PA Price</name></author><author><name sortKey="Stein, Wh" uniqKey="Stein W">WH Stein</name></author><author><name sortKey="Moore, S" uniqKey="Moore S">S Moore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Wj" uniqKey="Chen W">WJ Chen</name></author><author><name sortKey="Lee, Is" uniqKey="Lee I">IS Lee</name></author><author><name sortKey="Chen, Cy" uniqKey="Chen C">CY Chen</name></author><author><name sortKey="Liao, Th" uniqKey="Liao T">TH Liao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tullis, Rh" uniqKey="Tullis R">RH Tullis</name></author><author><name sortKey="Dill, Ka" uniqKey="Dill K">KA Dill</name></author><author><name sortKey="Price, Pa" uniqKey="Price P">PA Price</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jones, Sj" uniqKey="Jones S">SJ Jones</name></author><author><name sortKey="Worrall, Af" uniqKey="Worrall A">AF Worrall</name></author><author><name sortKey="Connolly, Ba" uniqKey="Connolly B">BA Connolly</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weston, Sa" uniqKey="Weston S">SA Weston</name></author><author><name sortKey="Lahm, A" uniqKey="Lahm A">A Lahm</name></author><author><name sortKey="Suck, D" uniqKey="Suck D">D Suck</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Wj" uniqKey="Chen W">WJ Chen</name></author><author><name sortKey="Lai, Pj" uniqKey="Lai P">PJ Lai</name></author><author><name sortKey="Lai, Ys" uniqKey="Lai Y">YS Lai</name></author><author><name sortKey="Huang, Pt" uniqKey="Huang P">PT Huang</name></author><author><name sortKey="Lin, Cc" uniqKey="Lin C">CC Lin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dupureur, Cm" uniqKey="Dupureur C">CM Dupureur</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lahm, A" uniqKey="Lahm A">A Lahm</name></author><author><name sortKey="Suck, D" uniqKey="Suck D">D Suck</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Glazer, Ds" uniqKey="Glazer D">DS Glazer</name></author><author><name sortKey="Radmer, Rj" uniqKey="Radmer R">RJ Radmer</name></author><author><name sortKey="Altman, Rb" uniqKey="Altman R">RB Altman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, Sh" uniqKey="Lee S">SH Lee</name></author><author><name sortKey="Kerff, F" uniqKey="Kerff F">F Kerff</name></author><author><name sortKey="Chereau, D" uniqKey="Chereau D">D Chereau</name></author><author><name sortKey="Ferron, F" uniqKey="Ferron F">F Ferron</name></author><author><name sortKey="Klug, A" uniqKey="Klug A">A Klug</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Suck, D" uniqKey="Suck D">D Suck</name></author><author><name sortKey="Oefner, C" uniqKey="Oefner C">C Oefner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bounds, Dg" uniqKey="Bounds D">DG Bounds</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jiao, D" uniqKey="Jiao D">D Jiao</name></author><author><name sortKey="King, C" uniqKey="King C">C King</name></author><author><name sortKey="Grossfield, A" uniqKey="Grossfield A">A Grossfield</name></author><author><name sortKey="Darden, Ta" uniqKey="Darden T">TA Darden</name></author><author><name sortKey="Ren, P" uniqKey="Ren P">P Ren</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Delalande, O" uniqKey="Delalande O">O Delalande</name></author><author><name sortKey="Ferey, N" uniqKey="Ferey N">N Ferey</name></author><author><name sortKey="Laurent, B" uniqKey="Laurent B">B Laurent</name></author><author><name sortKey="Gueroult, M" uniqKey="Gueroult M">M Gueroult</name></author><author><name sortKey="Hartmann, B" uniqKey="Hartmann B">B Hartmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Babor, M" uniqKey="Babor M">M Babor</name></author><author><name sortKey="Gerzon, S" uniqKey="Gerzon S">S Gerzon</name></author><author><name sortKey="Raveh, B" uniqKey="Raveh B">B Raveh</name></author><author><name sortKey="Sobolev, V" uniqKey="Sobolev V">V Sobolev</name></author><author><name sortKey="Edelman, M" uniqKey="Edelman M">M Edelman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pingoud, A" uniqKey="Pingoud A">A Pingoud</name></author><author><name sortKey="Fuxreiter, M" uniqKey="Fuxreiter M">M Fuxreiter</name></author><author><name sortKey="Pingoud, V" uniqKey="Pingoud V">V Pingoud</name></author><author><name sortKey="Wende, W" uniqKey="Wende W">W Wende</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sigel, Rk" uniqKey="Sigel R">RK Sigel</name></author><author><name sortKey="Pyle, Am" uniqKey="Pyle A">AM Pyle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, W" uniqKey="Yang W">W Yang</name></author><author><name sortKey="Lee, Jy" uniqKey="Lee J">JY Lee</name></author><author><name sortKey="Nowotny, M" uniqKey="Nowotny M">M Nowotny</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pingoud, V" uniqKey="Pingoud V">V Pingoud</name></author><author><name sortKey="Wende, W" uniqKey="Wende W">W Wende</name></author><author><name sortKey="Friedhoff, P" uniqKey="Friedhoff P">P Friedhoff</name></author><author><name sortKey="Reuter, M" uniqKey="Reuter M">M Reuter</name></author><author><name sortKey="Alves, J" uniqKey="Alves J">J Alves</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arcesi, L" uniqKey="Arcesi L">L Arcesi</name></author><author><name sortKey="La Penna, G" uniqKey="La Penna G">G La Penna</name></author><author><name sortKey="Perico, A" uniqKey="Perico A">A Perico</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cherstvy, Ag" uniqKey="Cherstvy A">AG Cherstvy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nadassy, K" uniqKey="Nadassy K">K Nadassy</name></author><author><name sortKey="Wodak, Sj" uniqKey="Wodak S">SJ Wodak</name></author><author><name sortKey="Janin, J" uniqKey="Janin J">J Janin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pan, Cq" uniqKey="Pan C">CQ Pan</name></author><author><name sortKey="Lazarus, Ra" uniqKey="Lazarus R">RA Lazarus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Evans, Sj" uniqKey="Evans S">SJ Evans</name></author><author><name sortKey="Shipstone, Ej" uniqKey="Shipstone E">EJ Shipstone</name></author><author><name sortKey="Maughan, Wn" uniqKey="Maughan W">WN Maughan</name></author><author><name sortKey="Connolly, Ba" uniqKey="Connolly B">BA Connolly</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kunitz, M" uniqKey="Kunitz M">M Kunitz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shack, J" uniqKey="Shack J">J Shack</name></author><author><name sortKey="Bynum, Bs" uniqKey="Bynum B">BS Bynum</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pan, Cq" 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uniqKey="Postma W">WF Postma</name></author><author><name sortKey="Van Gunsteren, A" uniqKey="Van Gunsteren A">A Van Gunsteren</name></author><author><name sortKey="Dinola, A" uniqKey="Dinola A">A DiNola</name></author><author><name sortKey="Haak, Jr" uniqKey="Haak J">JR Haak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Gunsteren, Wf" uniqKey="Van Gunsteren W">WF Van Gunsteren</name></author><author><name sortKey="Berendsen, Hjc" uniqKey="Berendsen H">HJC Berendsen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Darden, T" uniqKey="Darden T">T Darden</name></author><author><name sortKey="York, D" uniqKey="York D">D York</name></author><author><name sortKey="Pedersen, L" uniqKey="Pedersen L">L Pedersen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vriend, G" uniqKey="Vriend G">G Vriend</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grossfield, A" uniqKey="Grossfield A">A Grossfield</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baker, Na" uniqKey="Baker N">NA Baker</name></author><author><name sortKey="Sept, D" uniqKey="Sept D">D Sept</name></author><author><name sortKey="Joseph, S" uniqKey="Joseph S">S Joseph</name></author><author><name sortKey="Holst, Mj" uniqKey="Holst M">MJ Holst</name></author><author><name sortKey="Mccammon, Ja" uniqKey="Mccammon J">JA McCammon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hubbard, Sj" uniqKey="Hubbard S">SJ Hubbard</name></author><author><name sortKey="Thornton, Jm" uniqKey="Thornton J">JM Thornton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Prabhu, Nv" uniqKey="Prabhu N">NV Prabhu</name></author><author><name sortKey="Panda, M" uniqKey="Panda M">M Panda</name></author><author><name sortKey="Yang, Q" uniqKey="Yang Q">Q Yang</name></author><author><name sortKey="Sharp, Ka" uniqKey="Sharp K">KA Sharp</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Altschul, Sf" uniqKey="Altschul S">SF Altschul</name></author><author><name sortKey="Madden, Tl" uniqKey="Madden T">TL Madden</name></author><author><name sortKey="Schaffer, Aa" uniqKey="Schaffer A">AA Schaffer</name></author><author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name></author><author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thompson, Jd" uniqKey="Thompson J">JD Thompson</name></author><author><name sortKey="Higgins, Dg" uniqKey="Higgins D">DG Higgins</name></author><author><name sortKey="Gibson, Tj" uniqKey="Gibson T">TJ Gibson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Heinig, M" uniqKey="Heinig M">M Heinig</name></author><author><name sortKey="Frishman, D" uniqKey="Frishman D">D Frishman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Humphrey, W" uniqKey="Humphrey W">W Humphrey</name></author><author><name sortKey="Dalke, A" uniqKey="Dalke A">A Dalke</name></author><author><name sortKey="Schulten, K" uniqKey="Schulten K">K Schulten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saladin, A" uniqKey="Saladin A">A Saladin</name></author><author><name sortKey="Fiorucci, S" uniqKey="Fiorucci S">S Fiorucci</name></author><author><name sortKey="Poulain, P" uniqKey="Poulain P">P Poulain</name></author><author><name sortKey="Prevost, C" uniqKey="Prevost C">C Prevost</name></author><author><name sortKey="Zacharias, M" uniqKey="Zacharias M">M Zacharias</name></author></analytic></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>France</li></country><settlement><li>Paris</li></settlement></list><tree><country name="France"><noRegion><name sortKey="Gueroult, Marc" sort="Gueroult, Marc" uniqKey="Gueroult M" first="Marc" last="Guéroult">Marc Guéroult</name></noRegion><name sortKey="Abi Ghanem, Josephine" sort="Abi Ghanem, Josephine" uniqKey="Abi Ghanem J" first="Joséphine" last="Abi-Ghanem">Joséphine Abi-Ghanem</name><name sortKey="Baaden, Marc" sort="Baaden, Marc" uniqKey="Baaden M" first="Marc" last="Baaden">Marc Baaden</name><name sortKey="Gueroult, Marc" sort="Gueroult, Marc" uniqKey="Gueroult M" first="Marc" last="Guéroult">Marc Guéroult</name><name sortKey="Hartmann, Brigitte" sort="Hartmann, Brigitte" uniqKey="Hartmann B" first="Brigitte" last="Hartmann">Brigitte Hartmann</name><name sortKey="Picot, Daniel" sort="Picot, Daniel" uniqKey="Picot D" first="Daniel" last="Picot">Daniel Picot</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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