How Cations Can Assist DNase I in DNA Binding and Hydrolysis
Identifieur interne : 001791 ( Ncbi/Checkpoint ); précédent : 001790; suivant : 001792How Cations Can Assist DNase I in DNA Binding and Hydrolysis
Auteurs : Marc Guéroult [France] ; Daniel Picot [France] ; Joséphine Abi-Ghanem [France] ; Brigitte Hartmann [France] ; Marc Baaden [France]Source :
- PLoS Computational Biology [ 1553-734X ] ; 2010.
Abstract
DNase I requires Ca2+ and Mg2+ for hydrolyzing double-stranded DNA. However, the number and the location of DNase I ion-binding sites remain unclear, as well as the role of these counter-ions. Using molecular dynamics simulations, we show that bovine pancreatic (bp) DNase I contains four ion-binding pockets. Two of them strongly bind Ca2+ while the other two sites coordinate Mg2+. These theoretical results are strongly supported by revisiting crystallographic structures that contain bpDNase I. One Ca2+ stabilizes the functional DNase I structure. The presence of Mg2+ in close vicinity to the catalytic pocket of bpDNase I reinforces the idea of a cation-assisted hydrolytic mechanism. Importantly, Poisson-Boltzmann-type electrostatic potential calculations demonstrate that the divalent cations collectively control the electrostatic fit between bpDNase I and DNA. These results improve our understanding of the essential role of cations in the biological function of bpDNase I. The high degree of conservation of the amino acids involved in the identified cation-binding sites across DNase I and DNase I-like proteins from various species suggests that our findings generally apply to all DNase I-DNA interactions.
Url:
DOI: 10.1371/journal.pcbi.1001000
PubMed: 21124947
PubMed Central: 2987838
Affiliations:
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<front><div type="abstract" xml:lang="en"><p>DNase I requires Ca<sup>2+</sup>
and Mg<sup>2+</sup>
for hydrolyzing double-stranded DNA. However, the number and the location of DNase I ion-binding sites remain unclear, as well as the role of these counter-ions. Using molecular dynamics simulations, we show that bovine pancreatic (bp) DNase I contains four ion-binding pockets. Two of them strongly bind Ca<sup>2+</sup>
while the other two sites coordinate Mg<sup>2+</sup>
. These theoretical results are strongly supported by revisiting crystallographic structures that contain bpDNase I. One Ca<sup>2+</sup>
stabilizes the functional DNase I structure. The presence of Mg<sup>2+</sup>
in close vicinity to the catalytic pocket of bpDNase I reinforces the idea of a cation-assisted hydrolytic mechanism. Importantly, Poisson-Boltzmann-type electrostatic potential calculations demonstrate that the divalent cations collectively control the electrostatic fit between bpDNase I and DNA. These results improve our understanding of the essential role of cations in the biological function of bpDNase I. The high degree of conservation of the amino acids involved in the identified cation-binding sites across DNase I and DNase I-like proteins from various species suggests that our findings generally apply to all DNase I-DNA interactions.</p>
</div>
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