Influence of ePTFE polymer implant permeability on the rate and density of corneal extracellular matrix synthesis
Identifieur interne : 001B79 ( Istex/Corpus ); précédent : 001B78; suivant : 001B80Influence of ePTFE polymer implant permeability on the rate and density of corneal extracellular matrix synthesis
Auteurs : Jean Arc Legeais ; Isabelle Drubaix ; Benoit Briat ; Michèle Savoldelli ; Maurice Ménasche ; Ladislas Robert ; Gilles Renard ; Yves PouliquenSource :
- Journal of Biomedical Materials Research [ 0021-9304 ] ; 1997-07.
Abstract
Microporous polymers have great potential for the production of corneal keratoprosthetic devices. Keratocytes invade the pores of expanded polytetrafluoroethylene implants (ePTFE) and collagen synthesis occurs. This ePTFE becomes translucent after its implantation in the stroma of rabbit cornea. The rate and density of cell growth within this polymer depends on the implant thickness, pore size, and its placement in the cornea. We have investigated the influence of the polymer permeability on the collagen and protein contents of ePTFE implants. Rabbit corneal stroma were implanted with ePTFE disks (6 mm in diameter) by intralamellar keratoplasty. The implanted polymers were removed from the stroma after 3 or 6 months. The collagen and protein contents were determined after pepsin solubilization. The collagen content of the high‐permeability implant was 3.7‐fold greater than that of the low‐permeability implant 3 months after implantation and 2.4‐fold greater after 6 months. The total protein content of the high‐permeability implant was 2.5‐fold greater than that of the low‐permeability implant at 3 months and was the same after 6 months. The collagen‐to‐protein ratio was 68% in the high‐permeability implants, and thus similar to that of normal corneal stroma. Thus, high polymer permeability increased both the rate and density of the corneal extracellular matrix ingrowth. © 1997 John Wiley & Sons, Inc.
Url:
DOI: 10.1002/(SICI)1097-4636(199707)36:1<49::AID-JBM6>3.0.CO;2-L
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Biomed. Mater. Res.</title><idno type="ISSN">0021-9304</idno><idno type="eISSN">1097-4636</idno><imprint><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="1997-07">1997-07</date><biblScope unit="volume">36</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="49">49</biblScope><biblScope unit="page" to="54">54</biblScope></imprint><idno type="ISSN">0021-9304</idno></series><idno type="istex">9DB9426EB8E755BE571DB604FD8B2ADA66E31D2F</idno><idno type="DOI">10.1002/(SICI)1097-4636(199707)36:1<49::AID-JBM6>3.0.CO;2-L</idno><idno type="ArticleID">JBM6</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9304</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Microporous polymers have great potential for the production of corneal keratoprosthetic devices. Keratocytes invade the pores of expanded polytetrafluoroethylene implants (ePTFE) and collagen synthesis occurs. This ePTFE becomes translucent after its implantation in the stroma of rabbit cornea. The rate and density of cell growth within this polymer depends on the implant thickness, pore size, and its placement in the cornea. We have investigated the influence of the polymer permeability on the collagen and protein contents of ePTFE implants. Rabbit corneal stroma were implanted with ePTFE disks (6 mm in diameter) by intralamellar keratoplasty. The implanted polymers were removed from the stroma after 3 or 6 months. The collagen and protein contents were determined after pepsin solubilization. The collagen content of the high‐permeability implant was 3.7‐fold greater than that of the low‐permeability implant 3 months after implantation and 2.4‐fold greater after 6 months. The total protein content of the high‐permeability implant was 2.5‐fold greater than that of the low‐permeability implant at 3 months and was the same after 6 months. The collagen‐to‐protein ratio was 68% in the high‐permeability implants, and thus similar to that of normal corneal stroma. Thus, high polymer permeability increased both the rate and density of the corneal extracellular matrix ingrowth. © 1997 John Wiley & Sons, Inc.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Jean‐Marc Legeais</name><affiliations><json:string>Laboratoire de Biochimie des Tissus Conjonctifs, Université Paris VII, Paris, France</json:string></affiliations></json:item><json:item><name>Isabelle Drubaix</name><affiliations><json:string>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</json:string><json:string>Laboratoire de Biochimie des Tissus Conjonctifs, Université Paris VII, Paris, France</json:string></affiliations></json:item><json:item><name>Benoit Briat</name><affiliations><json:string>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</json:string></affiliations></json:item><json:item><name>Michèle Savoldelli</name><affiliations><json:string>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</json:string></affiliations></json:item><json:item><name>Maurice Ménasche</name><affiliations><json:string>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</json:string></affiliations></json:item><json:item><name>Ladislas Robert</name><affiliations><json:string>Laboratoire de Biochimie des Tissus Conjonctifs, Université Paris VII, Paris, France</json:string></affiliations></json:item><json:item><name>Gilles Renard</name><affiliations><json:string>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</json:string></affiliations></json:item><json:item><name>Yves Pouliquen</name><affiliations><json:string>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</json:string></affiliations></json:item></author><articleId><json:string>JBM6</json:string></articleId><language><json:string>eng</json:string></language><abstract>Microporous polymers have great potential for the production of corneal keratoprosthetic devices. Keratocytes invade the pores of expanded polytetrafluoroethylene implants (ePTFE) and collagen synthesis occurs. This ePTFE becomes translucent after its implantation in the stroma of rabbit cornea. The rate and density of cell growth within this polymer depends on the implant thickness, pore size, and its placement in the cornea. We have investigated the influence of the polymer permeability on the collagen and protein contents of ePTFE implants. Rabbit corneal stroma were implanted with ePTFE disks (6 mm in diameter) by intralamellar keratoplasty. The implanted polymers were removed from the stroma after 3 or 6 months. The collagen and protein contents were determined after pepsin solubilization. The collagen content of the high‐permeability implant was 3.7‐fold greater than that of the low‐permeability implant 3 months after implantation and 2.4‐fold greater after 6 months. The total protein content of the high‐permeability implant was 2.5‐fold greater than that of the low‐permeability implant at 3 months and was the same after 6 months. The collagen‐to‐protein ratio was 68% in the high‐permeability implants, and thus similar to that of normal corneal stroma. Thus, high polymer permeability increased both the rate and density of the corneal extracellular matrix ingrowth. © 1997 John Wiley & Sons, Inc.</abstract><qualityIndicators><score>5.765</score><pdfVersion>1.3</pdfVersion><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>1423</abstractCharCount><pdfWordCount>3233</pdfWordCount><pdfCharCount>20105</pdfCharCount><pdfPageCount>6</pdfPageCount><abstractWordCount>211</abstractWordCount></qualityIndicators><title>Influence of ePTFE polymer implant permeability on the rate and density of corneal extracellular matrix 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corneal extracellular matrix synthesis</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><availability><p>WILEY</p></availability><date>1997</date></publicationStmt><notesStmt><note>Délé á la Reacharahe clinique</note><note>Fondation de l'Avenir</note><note>INSERM</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Influence of ePTFE polymer implant permeability on the rate and density of corneal extracellular matrix synthesis</title><author><persName><forename type="first">Jean‐Marc</forename><surname>Legeais</surname></persName><note type="correspondence"><p>Correspondence: Laboratoire de Biochimie des Tissus Conjonctifs, Université Paris VII, Paris, France</p></note><affiliation>Laboratoire de Biochimie des Tissus Conjonctifs, Université Paris VII, Paris, France</affiliation></author><author><persName><forename type="first">Isabelle</forename><surname>Drubaix</surname></persName><affiliation>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</affiliation><affiliation>Laboratoire de Biochimie des Tissus Conjonctifs, Université Paris VII, Paris, France</affiliation></author><author><persName><forename type="first">Benoit</forename><surname>Briat</surname></persName><affiliation>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</affiliation></author><author><persName><forename type="first">Michèle</forename><surname>Savoldelli</surname></persName><affiliation>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</affiliation></author><author><persName><forename type="first">Maurice</forename><surname>Ménasche</surname></persName><affiliation>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</affiliation></author><author><persName><forename type="first">Ladislas</forename><surname>Robert</surname></persName><affiliation>Laboratoire de Biochimie des Tissus Conjonctifs, Université Paris VII, Paris, France</affiliation></author><author><persName><forename type="first">Gilles</forename><surname>Renard</surname></persName><affiliation>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</affiliation></author><author><persName><forename type="first">Yves</forename><surname>Pouliquen</surname></persName><affiliation>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</affiliation></author></analytic><monogr><title level="j">Journal of Biomedical Materials Research</title><title level="j" type="sub">An Official Journal of The Society for Biomaterials and The Japanese Society for Biomaterials</title><title level="j" type="abbrev">J. Biomed. Mater. Res.</title><idno type="pISSN">0021-9304</idno><idno type="eISSN">1097-4636</idno><idno type="DOI">10.1002/(ISSN)1097-4636</idno><imprint><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="1997-07"></date><biblScope unit="volume">36</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="49">49</biblScope><biblScope unit="page" to="54">54</biblScope></imprint></monogr><idno type="istex">9DB9426EB8E755BE571DB604FD8B2ADA66E31D2F</idno><idno type="DOI">10.1002/(SICI)1097-4636(199707)36:1<49::AID-JBM6>3.0.CO;2-L</idno><idno type="ArticleID">JBM6</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1997</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Microporous polymers have great potential for the production of corneal keratoprosthetic devices. Keratocytes invade the pores of expanded polytetrafluoroethylene implants (ePTFE) and collagen synthesis occurs. This ePTFE becomes translucent after its implantation in the stroma of rabbit cornea. The rate and density of cell growth within this polymer depends on the implant thickness, pore size, and its placement in the cornea. We have investigated the influence of the polymer permeability on the collagen and protein contents of ePTFE implants. Rabbit corneal stroma were implanted with ePTFE disks (6 mm in diameter) by intralamellar keratoplasty. The implanted polymers were removed from the stroma after 3 or 6 months. The collagen and protein contents were determined after pepsin solubilization. The collagen content of the high‐permeability implant was 3.7‐fold greater than that of the low‐permeability implant 3 months after implantation and 2.4‐fold greater after 6 months. The total protein content of the high‐permeability implant was 2.5‐fold greater than that of the low‐permeability implant at 3 months and was the same after 6 months. The collagen‐to‐protein ratio was 68% in the high‐permeability implants, and thus similar to that of normal corneal stroma. Thus, high polymer permeability increased both the rate and density of the corneal extracellular matrix ingrowth. © 1997 John Wiley & Sons, Inc.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Research Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1995-08-16">Received</change><change when="1996-06-10">Registration</change><change when="1997-07">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/9DB9426EB8E755BE571DB604FD8B2ADA66E31D2F/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>John Wiley & Sons, Inc.</publisherName><publisherLoc>New York</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1097-4636</doi><issn type="print">0021-9304</issn><issn type="electronic">1097-4636</issn><idGroup><id type="product" value="JBM"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="JOURNAL OF BIOMEDICAL MATERIALS RESEARCH">Journal of Biomedical Materials Research</title><title type="subtitle">An Official Journal of The Society for Biomaterials and The Japanese Society for Biomaterials</title><title type="short">J. 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Res.</title></titleGroup><selfCitationGroup><citation type="ancestor" xml:id="cit1"><journalTitle>Journal of Applied Biomaterials</journalTitle><accessionId ref="info:x-wiley/issn/10454861">1045-4861</accessionId><pubYear year="1995">1995</pubYear><vol>6</vol><issue>4</issue></citation></selfCitationGroup></publicationMeta><publicationMeta level="part" position="10"><doi origin="wiley" registered="yes">10.1002/(SICI)1097-4636(199707)36:1<>1.0.CO;2-I</doi><idGroup><id type="focusSection" value="0"></id></idGroup><titleGroup><title type="focusSection" xml:lang="en">Journal of Biomedical Materials Research</title></titleGroup><numberingGroup><numbering type="journalVolume" number="36">36</numbering><numbering type="journalIssue">1</numbering></numberingGroup><coverDate startDate="1997-07">July 1997</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="6" status="forIssue"><doi origin="wiley" registered="yes">10.1002/(SICI)1097-4636(199707)36:1<49::AID-JBM6>3.0.CO;2-L</doi><idGroup><id type="unit" value="JBM6"></id></idGroup><countGroup><count type="pageTotal" number="6"></count></countGroup><titleGroup><title type="articleCategory">Research Article</title><title type="tocHeading1">Research Articles</title></titleGroup><copyright ownership="publisher">Copyright © 1997 John Wiley & Sons, Inc.</copyright><eventGroup><event type="manuscriptReceived" date="1995-08-16"></event><event type="manuscriptAccepted" date="1996-06-10"></event><event type="firstOnline" date="1998-12-06"></event><event type="publishedOnlineFinalForm" date="1998-12-06"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.6 mode:FullText" date="2010-05-13"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-28"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-23"></event></eventGroup><numberingGroup><numbering type="pageFirst">49</numbering><numbering type="pageLast">54</numbering></numberingGroup><correspondenceTo>Laboratoire de Biochimie des Tissus Conjonctifs, Université Paris VII, Paris, France</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JBM.JBM6.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="6"></count><count type="tableTotal" number="0"></count><count type="referenceTotal" number="30"></count></countGroup><titleGroup><title type="main" xml:lang="en">Influence of ePTFE polymer implant permeability on the rate and density of corneal extracellular matrix synthesis</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Jean‐Marc</givenNames><familyName>Legeais</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af2 #af1"><personName><givenNames>Isabelle</givenNames><familyName>Drubaix</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Benoit</givenNames><familyName>Briat</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Michèle</givenNames><familyName>Savoldelli</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Maurice</givenNames><familyName>Ménasche</familyName></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Ladislas</givenNames><familyName>Robert</familyName></personName></creator><creator xml:id="au7" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Gilles</givenNames><familyName>Renard</familyName></personName></creator><creator xml:id="au8" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Yves</givenNames><familyName>Pouliquen</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="FR" type="organization"><unparsedAffiliation>Laboratoire de Biochimie des Tissus Conjonctifs, Université Paris VII, Paris, France</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="FR" type="organization"><unparsedAffiliation>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</unparsedAffiliation></affiliation></affiliationGroup><fundingInfo><fundingAgency>Délé á la Reacharahe clinique</fundingAgency></fundingInfo><fundingInfo><fundingAgency>Fondation de l'Avenir</fundingAgency></fundingInfo><fundingInfo><fundingAgency>INSERM</fundingAgency></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Microporous polymers have great potential for the production of corneal keratoprosthetic devices. Keratocytes invade the pores of expanded polytetrafluoroethylene implants (ePTFE) and collagen synthesis occurs. This ePTFE becomes translucent after its implantation in the stroma of rabbit cornea. The rate and density of cell growth within this polymer depends on the implant thickness, pore size, and its placement in the cornea. We have investigated the influence of the polymer permeability on the collagen and protein contents of ePTFE implants. Rabbit corneal stroma were implanted with ePTFE disks (6 mm in diameter) by intralamellar keratoplasty. The implanted polymers were removed from the stroma after 3 or 6 months. The collagen and protein contents were determined after pepsin solubilization. The collagen content of the high‐permeability implant was 3.7‐fold greater than that of the low‐permeability implant 3 months after implantation and 2.4‐fold greater after 6 months. The total protein content of the high‐permeability implant was 2.5‐fold greater than that of the low‐permeability implant at 3 months and was the same after 6 months. The collagen‐to‐protein ratio was 68% in the high‐permeability implants, and thus similar to that of normal corneal stroma. Thus, high polymer permeability increased both the rate and density of the corneal extracellular matrix ingrowth. © 1997 John Wiley & Sons, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Influence of ePTFE polymer implant permeability on the rate and density of corneal extracellular matrix synthesis</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Influence of ePTFE polymer implant permeability on the rate and density of corneal extracellular matrix synthesis</title></titleInfo><name type="personal"><namePart type="given">Jean‐Marc</namePart><namePart type="family">Legeais</namePart><affiliation>Laboratoire de Biochimie des Tissus Conjonctifs, Université Paris VII, Paris, France</affiliation><description>Correspondence: Laboratoire de Biochimie des Tissus Conjonctifs, Université Paris VII, Paris, France</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Isabelle</namePart><namePart type="family">Drubaix</namePart><affiliation>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</affiliation><affiliation>Laboratoire de Biochimie des Tissus Conjonctifs, Université Paris VII, Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Benoit</namePart><namePart type="family">Briat</namePart><affiliation>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Michèle</namePart><namePart type="family">Savoldelli</namePart><affiliation>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Maurice</namePart><namePart type="family">Ménasche</namePart><affiliation>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ladislas</namePart><namePart type="family">Robert</namePart><affiliation>Laboratoire de Biochimie des Tissus Conjonctifs, Université Paris VII, Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Gilles</namePart><namePart type="family">Renard</namePart><affiliation>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yves</namePart><namePart type="family">Pouliquen</namePart><affiliation>Département d'Ophtalmologie Hôtel‐Dieu de Paris, INSERM U 86, 1 Place du Parvis Nôtre Dame, 75004 Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>John Wiley & Sons, Inc.</publisher><place><placeTerm type="text">New York</placeTerm></place><dateIssued encoding="w3cdtf">1997-07</dateIssued><dateCaptured encoding="w3cdtf">1995-08-16</dateCaptured><dateValid encoding="w3cdtf">1996-06-10</dateValid><copyrightDate encoding="w3cdtf">1997</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">6</extent><extent unit="references">30</extent></physicalDescription><abstract lang="en">Microporous polymers have great potential for the production of corneal keratoprosthetic devices. Keratocytes invade the pores of expanded polytetrafluoroethylene implants (ePTFE) and collagen synthesis occurs. This ePTFE becomes translucent after its implantation in the stroma of rabbit cornea. The rate and density of cell growth within this polymer depends on the implant thickness, pore size, and its placement in the cornea. We have investigated the influence of the polymer permeability on the collagen and protein contents of ePTFE implants. Rabbit corneal stroma were implanted with ePTFE disks (6 mm in diameter) by intralamellar keratoplasty. The implanted polymers were removed from the stroma after 3 or 6 months. The collagen and protein contents were determined after pepsin solubilization. The collagen content of the high‐permeability implant was 3.7‐fold greater than that of the low‐permeability implant 3 months after implantation and 2.4‐fold greater after 6 months. The total protein content of the high‐permeability implant was 2.5‐fold greater than that of the low‐permeability implant at 3 months and was the same after 6 months. The collagen‐to‐protein ratio was 68% in the high‐permeability implants, and thus similar to that of normal corneal stroma. Thus, high polymer permeability increased both the rate and density of the corneal extracellular matrix ingrowth. © 1997 John Wiley & Sons, Inc.</abstract><note type="funding">Délé á la Reacharahe clinique</note><note type="funding">Fondation de l'Avenir</note><note type="funding">INSERM</note><relatedItem type="host"><titleInfo><title>Journal of Biomedical Materials Research</title><subTitle>An Official Journal of The Society for Biomaterials and The Japanese Society for Biomaterials</subTitle></titleInfo><titleInfo type="abbreviated"><title>J. Biomed. Mater. Res.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Research Article</topic></subject><identifier type="ISSN">0021-9304</identifier><identifier type="eISSN">1097-4636</identifier><identifier type="DOI">10.1002/(ISSN)1097-4636</identifier><identifier type="PublisherID">JBM</identifier><part><date>1997</date><detail type="volume"><caption>vol.</caption><number>36</number></detail><detail type="issue"><caption>no.</caption><number>1</number></detail><extent unit="pages"><start>49</start><end>54</end><total>6</total></extent></part></relatedItem><relatedItem type="preceding"><titleInfo><title>Journal of Applied Biomaterials</title></titleInfo><identifier type="ISSN">1045-4861</identifier><part><date point="end">1995</date><detail type="volume"><caption>last vol.</caption><number>6</number></detail><detail type="issue"><caption>last no.</caption><number>4</number></detail></part></relatedItem><identifier type="istex">9DB9426EB8E755BE571DB604FD8B2ADA66E31D2F</identifier><identifier type="DOI">10.1002/(SICI)1097-4636(199707)36:1<49::AID-JBM6>3.0.CO;2-L</identifier><identifier type="ArticleID">JBM6</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 1997 John Wiley & Sons, Inc.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>John Wiley & Sons, Inc.</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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