Proteomics show antigen presentation processes in human immune cells after AS03-H5N1 vaccination.
Identifieur interne : 000316 ( PubMed/Curation ); précédent : 000315; suivant : 000317Proteomics show antigen presentation processes in human immune cells after AS03-H5N1 vaccination.
Auteurs : Allison C. Galassie [États-Unis] ; Johannes B. Goll [États-Unis] ; Parimal Samir [États-Unis] ; Travis L. Jensen [États-Unis] ; Kristen L. Hoek [États-Unis] ; Leigh M. Howard [États-Unis] ; Tara M. Allos [États-Unis] ; Xinnan Niu [États-Unis] ; Laura E. Gordy [États-Unis] ; C Buddy Creech [États-Unis] ; Heather Hill [États-Unis] ; Sebastian Joyce [États-Unis] ; Kathryn M. Edwards [États-Unis] ; Andrew J. Link [États-Unis]Source :
- Proteomics [ 1615-9861 ] ; 2017.
Descripteurs français
- KwdFr :
- Adjuvants immunologiques, Cartes d'interactions protéiques, Cellules cultivées, Cellules tueuses naturelles (cytologie), Cellules tueuses naturelles (immunologie), Cellules tueuses naturelles (métabolisme), Granulocytes neutrophiles (cytologie), Granulocytes neutrophiles (immunologie), Granulocytes neutrophiles (métabolisme), Grippe humaine (), Grippe humaine (immunologie), Humains, Lymphocytes B (cytologie), Lymphocytes B (immunologie), Lymphocytes B (métabolisme), Lymphocytes T (cytologie), Lymphocytes T (immunologie), Lymphocytes T (métabolisme), Monocytes (cytologie), Monocytes (immunologie), Monocytes (métabolisme), Protéome (métabolisme), Protéomique, Présentation d'antigène, Sous-type H5N1 du virus de la grippe A (immunologie), Vaccins antigrippaux (usage thérapeutique).
- MESH :
- cytologie : Cellules tueuses naturelles, Granulocytes neutrophiles, Lymphocytes B, Lymphocytes T, Monocytes.
- immunologie : Cellules tueuses naturelles, Granulocytes neutrophiles, Grippe humaine, Lymphocytes B, Lymphocytes T, Monocytes, Sous-type H5N1 du virus de la grippe A.
- métabolisme : Cellules tueuses naturelles, Granulocytes neutrophiles, Lymphocytes B, Lymphocytes T, Monocytes, Protéome.
- usage thérapeutique : Vaccins antigrippaux.
- Adjuvants immunologiques, Cartes d'interactions protéiques, Cellules cultivées, Grippe humaine, Humains, Protéomique, Présentation d'antigène.
English descriptors
- KwdEn :
- Adjuvants, Immunologic, Antigen Presentation, B-Lymphocytes (cytology), B-Lymphocytes (immunology), B-Lymphocytes (metabolism), Cells, Cultured, Humans, Influenza A Virus, H5N1 Subtype (immunology), Influenza Vaccines (therapeutic use), Influenza, Human (immunology), Influenza, Human (prevention & control), Killer Cells, Natural (cytology), Killer Cells, Natural (immunology), Killer Cells, Natural (metabolism), Monocytes (cytology), Monocytes (immunology), Monocytes (metabolism), Neutrophils (cytology), Neutrophils (immunology), Neutrophils (metabolism), Protein Interaction Maps, Proteome (metabolism), Proteomics, T-Lymphocytes (cytology), T-Lymphocytes (immunology), T-Lymphocytes (metabolism).
- MESH :
- chemical , metabolism : Proteome.
- chemical , therapeutic use : Influenza Vaccines.
- chemical : Adjuvants, Immunologic.
- cytology : B-Lymphocytes, Killer Cells, Natural, Monocytes, Neutrophils, T-Lymphocytes.
- immunology : B-Lymphocytes, Influenza A Virus, H5N1 Subtype, Influenza, Human, Killer Cells, Natural, Monocytes, Neutrophils, T-Lymphocytes.
- metabolism : B-Lymphocytes, Killer Cells, Natural, Monocytes, Neutrophils, T-Lymphocytes.
- prevention & control : Influenza, Human.
- Antigen Presentation, Cells, Cultured, Humans, Protein Interaction Maps, Proteomics.
Abstract
Adjuvants enhance immunity elicited by vaccines through mechanisms that are poorly understood. Using a systems biology approach, we investigated temporal protein expression changes in five primary human immune cell populations: neutrophils, monocytes, natural killer cells, T cells, and B cells after administration of either an Adjuvant System 03 adjuvanted or unadjuvanted split-virus H5N1 influenza vaccine. Monocytes demonstrated the strongest differential signal between vaccine groups. On day 3 post-vaccination, several antigen presentation-related pathways, including MHC class I-mediated antigen processing and presentation, were enriched in monocytes and neutrophils and expression of HLA class I proteins was increased in the Adjuvant System 03 group. We identified several protein families whose proteomic responses predicted seroprotective antibody responses (>1:40 hemagglutination inhibition titer), including inflammation and oxidative stress proteins at day 1 as well as immunoproteasome subunit (PSME1 and PSME2) and HLA class I proteins at day 3 in monocytes. While comparison between temporal proteomic and transcriptomic results showed little overlap overall, enrichment of the MHC class I antigen processing and presentation pathway in monocytes and neutrophils was confirmed by both approaches.
DOI: 10.1002/pmic.201600453
PubMed: 28508465
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea>
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<author><name sortKey="Howard, Leigh M" sort="Howard, Leigh M" uniqKey="Howard L" first="Leigh M" last="Howard">Leigh M. Howard</name>
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<author><name sortKey="Allos, Tara M" sort="Allos, Tara M" uniqKey="Allos T" first="Tara M" last="Allos">Tara M. Allos</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea>
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<author><name sortKey="Niu, Xinnan" sort="Niu, Xinnan" uniqKey="Niu X" first="Xinnan" last="Niu">Xinnan Niu</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea>
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<author><name sortKey="Gordy, Laura E" sort="Gordy, Laura E" uniqKey="Gordy L" first="Laura E" last="Gordy">Laura E. Gordy</name>
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<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Creech, C Buddy" sort="Creech, C Buddy" uniqKey="Creech C" first="C Buddy" last="Creech">C Buddy Creech</name>
<affiliation wicri:level="1"><nlm:affiliation>Vanderbilt Vaccine Research Program, Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vanderbilt Vaccine Research Program, Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hill, Heather" sort="Hill, Heather" uniqKey="Hill H" first="Heather" last="Hill">Heather Hill</name>
<affiliation wicri:level="1"><nlm:affiliation>The Emmes Corporation, Rockville, MD, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>The Emmes Corporation, Rockville, MD</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Joyce, Sebastian" sort="Joyce, Sebastian" uniqKey="Joyce S" first="Sebastian" last="Joyce">Sebastian Joyce</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Edwards, Kathryn M" sort="Edwards, Kathryn M" uniqKey="Edwards K" first="Kathryn M" last="Edwards">Kathryn M. Edwards</name>
<affiliation wicri:level="1"><nlm:affiliation>Vanderbilt Vaccine Research Program, Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vanderbilt Vaccine Research Program, Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Link, Andrew J" sort="Link, Andrew J" uniqKey="Link A" first="Andrew J" last="Link">Andrew J. Link</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, Vanderbilt University, Nashville, TN, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Chemistry, Vanderbilt University, Nashville, TN</wicri:regionArea>
</affiliation>
</author>
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<term>Antigen Presentation</term>
<term>B-Lymphocytes (cytology)</term>
<term>B-Lymphocytes (immunology)</term>
<term>B-Lymphocytes (metabolism)</term>
<term>Cells, Cultured</term>
<term>Humans</term>
<term>Influenza A Virus, H5N1 Subtype (immunology)</term>
<term>Influenza Vaccines (therapeutic use)</term>
<term>Influenza, Human (immunology)</term>
<term>Influenza, Human (prevention & control)</term>
<term>Killer Cells, Natural (cytology)</term>
<term>Killer Cells, Natural (immunology)</term>
<term>Killer Cells, Natural (metabolism)</term>
<term>Monocytes (cytology)</term>
<term>Monocytes (immunology)</term>
<term>Monocytes (metabolism)</term>
<term>Neutrophils (cytology)</term>
<term>Neutrophils (immunology)</term>
<term>Neutrophils (metabolism)</term>
<term>Protein Interaction Maps</term>
<term>Proteome (metabolism)</term>
<term>Proteomics</term>
<term>T-Lymphocytes (cytology)</term>
<term>T-Lymphocytes (immunology)</term>
<term>T-Lymphocytes (metabolism)</term>
</keywords>
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<term>Cartes d'interactions protéiques</term>
<term>Cellules cultivées</term>
<term>Cellules tueuses naturelles (cytologie)</term>
<term>Cellules tueuses naturelles (immunologie)</term>
<term>Cellules tueuses naturelles (métabolisme)</term>
<term>Granulocytes neutrophiles (cytologie)</term>
<term>Granulocytes neutrophiles (immunologie)</term>
<term>Granulocytes neutrophiles (métabolisme)</term>
<term>Grippe humaine ()</term>
<term>Grippe humaine (immunologie)</term>
<term>Humains</term>
<term>Lymphocytes B (cytologie)</term>
<term>Lymphocytes B (immunologie)</term>
<term>Lymphocytes B (métabolisme)</term>
<term>Lymphocytes T (cytologie)</term>
<term>Lymphocytes T (immunologie)</term>
<term>Lymphocytes T (métabolisme)</term>
<term>Monocytes (cytologie)</term>
<term>Monocytes (immunologie)</term>
<term>Monocytes (métabolisme)</term>
<term>Protéome (métabolisme)</term>
<term>Protéomique</term>
<term>Présentation d'antigène</term>
<term>Sous-type H5N1 du virus de la grippe A (immunologie)</term>
<term>Vaccins antigrippaux (usage thérapeutique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Proteome</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Influenza Vaccines</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Adjuvants, Immunologic</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cellules tueuses naturelles</term>
<term>Granulocytes neutrophiles</term>
<term>Lymphocytes B</term>
<term>Lymphocytes T</term>
<term>Monocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>B-Lymphocytes</term>
<term>Killer Cells, Natural</term>
<term>Monocytes</term>
<term>Neutrophils</term>
<term>T-Lymphocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Cellules tueuses naturelles</term>
<term>Granulocytes neutrophiles</term>
<term>Grippe humaine</term>
<term>Lymphocytes B</term>
<term>Lymphocytes T</term>
<term>Monocytes</term>
<term>Sous-type H5N1 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>B-Lymphocytes</term>
<term>Influenza A Virus, H5N1 Subtype</term>
<term>Influenza, Human</term>
<term>Killer Cells, Natural</term>
<term>Monocytes</term>
<term>Neutrophils</term>
<term>T-Lymphocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>B-Lymphocytes</term>
<term>Killer Cells, Natural</term>
<term>Monocytes</term>
<term>Neutrophils</term>
<term>T-Lymphocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules tueuses naturelles</term>
<term>Granulocytes neutrophiles</term>
<term>Lymphocytes B</term>
<term>Lymphocytes T</term>
<term>Monocytes</term>
<term>Protéome</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Vaccins antigrippaux</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Antigen Presentation</term>
<term>Cells, Cultured</term>
<term>Humans</term>
<term>Protein Interaction Maps</term>
<term>Proteomics</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Adjuvants immunologiques</term>
<term>Cartes d'interactions protéiques</term>
<term>Cellules cultivées</term>
<term>Grippe humaine</term>
<term>Humains</term>
<term>Protéomique</term>
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<front><div type="abstract" xml:lang="en">Adjuvants enhance immunity elicited by vaccines through mechanisms that are poorly understood. Using a systems biology approach, we investigated temporal protein expression changes in five primary human immune cell populations: neutrophils, monocytes, natural killer cells, T cells, and B cells after administration of either an Adjuvant System 03 adjuvanted or unadjuvanted split-virus H5N1 influenza vaccine. Monocytes demonstrated the strongest differential signal between vaccine groups. On day 3 post-vaccination, several antigen presentation-related pathways, including MHC class I-mediated antigen processing and presentation, were enriched in monocytes and neutrophils and expression of HLA class I proteins was increased in the Adjuvant System 03 group. We identified several protein families whose proteomic responses predicted seroprotective antibody responses (>1:40 hemagglutination inhibition titer), including inflammation and oxidative stress proteins at day 1 as well as immunoproteasome subunit (PSME1 and PSME2) and HLA class I proteins at day 3 in monocytes. While comparison between temporal proteomic and transcriptomic results showed little overlap overall, enrichment of the MHC class I antigen processing and presentation pathway in monocytes and neutrophils was confirmed by both approaches.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28508465</PMID>
<DateCompleted><Year>2017</Year>
<Month>11</Month>
<Day>20</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1615-9861</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>17</Volume>
<Issue>12</Issue>
<PubDate><Year>2017</Year>
<Month>Jun</Month>
</PubDate>
</JournalIssue>
<Title>Proteomics</Title>
<ISOAbbreviation>Proteomics</ISOAbbreviation>
</Journal>
<ArticleTitle>Proteomics show antigen presentation processes in human immune cells after AS03-H5N1 vaccination.</ArticleTitle>
<ELocationID EIdType="doi" ValidYN="Y">10.1002/pmic.201600453</ELocationID>
<Abstract><AbstractText>Adjuvants enhance immunity elicited by vaccines through mechanisms that are poorly understood. Using a systems biology approach, we investigated temporal protein expression changes in five primary human immune cell populations: neutrophils, monocytes, natural killer cells, T cells, and B cells after administration of either an Adjuvant System 03 adjuvanted or unadjuvanted split-virus H5N1 influenza vaccine. Monocytes demonstrated the strongest differential signal between vaccine groups. On day 3 post-vaccination, several antigen presentation-related pathways, including MHC class I-mediated antigen processing and presentation, were enriched in monocytes and neutrophils and expression of HLA class I proteins was increased in the Adjuvant System 03 group. We identified several protein families whose proteomic responses predicted seroprotective antibody responses (>1:40 hemagglutination inhibition titer), including inflammation and oxidative stress proteins at day 1 as well as immunoproteasome subunit (PSME1 and PSME2) and HLA class I proteins at day 3 in monocytes. While comparison between temporal proteomic and transcriptomic results showed little overlap overall, enrichment of the MHC class I antigen processing and presentation pathway in monocytes and neutrophils was confirmed by both approaches.</AbstractText>
<CopyrightInformation>© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Galassie</LastName>
<ForeName>Allison C</ForeName>
<Initials>AC</Initials>
<AffiliationInfo><Affiliation>Department of Chemistry, Vanderbilt University, Nashville, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Goll</LastName>
<ForeName>Johannes B</ForeName>
<Initials>JB</Initials>
<AffiliationInfo><Affiliation>The Emmes Corporation, Rockville, MD, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Samir</LastName>
<ForeName>Parimal</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jensen</LastName>
<ForeName>Travis L</ForeName>
<Initials>TL</Initials>
<AffiliationInfo><Affiliation>The Emmes Corporation, Rockville, MD, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hoek</LastName>
<ForeName>Kristen L</ForeName>
<Initials>KL</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Howard</LastName>
<ForeName>Leigh M</ForeName>
<Initials>LM</Initials>
<AffiliationInfo><Affiliation>Vanderbilt Vaccine Research Program, Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Allos</LastName>
<ForeName>Tara M</ForeName>
<Initials>TM</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Niu</LastName>
<ForeName>Xinnan</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gordy</LastName>
<ForeName>Laura E</ForeName>
<Initials>LE</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Creech</LastName>
<ForeName>C Buddy</ForeName>
<Initials>CB</Initials>
<AffiliationInfo><Affiliation>Vanderbilt Vaccine Research Program, Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hill</LastName>
<ForeName>Heather</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>The Emmes Corporation, Rockville, MD, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Joyce</LastName>
<ForeName>Sebastian</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Veterans Administration Tennessee Valley Healthcare System, Nashville, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Edwards</LastName>
<ForeName>Kathryn M</ForeName>
<Initials>KM</Initials>
<AffiliationInfo><Affiliation>Vanderbilt Vaccine Research Program, Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Link</LastName>
<ForeName>Andrew J</ForeName>
<Initials>AJ</Initials>
<AffiliationInfo><Affiliation>Department of Chemistry, Vanderbilt University, Nashville, TN, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>UL1 TR000445</GrantID>
<Acronym>TR</Acronym>
<Agency>NCATS NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>I01 BX001444</GrantID>
<Acronym>BX</Acronym>
<Agency>BLRD VA</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>T32 AI095202</GrantID>
<Acronym>AI</Acronym>
<Agency>NIAID NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>S10 RR024610</GrantID>
<Acronym>RR</Acronym>
<Agency>NCRR NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>T32 HL069765</GrantID>
<Acronym>HL</Acronym>
<Agency>NHLBI NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>R01 GM064779</GrantID>
<Acronym>GM</Acronym>
<Agency>NIGMS NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>UL1 RR024975</GrantID>
<Acronym>RR</Acronym>
<Agency>NCRR NIH HHS</Agency>
<Country>United States</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>Germany</Country>
<MedlineTA>Proteomics</MedlineTA>
<NlmUniqueID>101092707</NlmUniqueID>
<ISSNLinking>1615-9853</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000276">Adjuvants, Immunologic</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D007252">Influenza Vaccines</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D020543">Proteome</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000276" MajorTopicYN="N">Adjuvants, Immunologic</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017951" MajorTopicYN="Y">Antigen Presentation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001402" MajorTopicYN="N">B-Lymphocytes</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D053124" MajorTopicYN="N">Influenza A Virus, H5N1 Subtype</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007252" MajorTopicYN="N">Influenza Vaccines</DescriptorName>
<QualifierName UI="Q000627" MajorTopicYN="Y">therapeutic use</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000517" MajorTopicYN="N">prevention & control</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007694" MajorTopicYN="N">Killer Cells, Natural</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009000" MajorTopicYN="N">Monocytes</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009504" MajorTopicYN="N">Neutrophils</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060066" MajorTopicYN="N">Protein Interaction Maps</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013601" MajorTopicYN="N">T-Lymphocytes</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">AS03</Keyword>
<Keyword MajorTopicYN="N">Avian influenza</Keyword>
<Keyword MajorTopicYN="N">Immune response</Keyword>
<Keyword MajorTopicYN="N">Quantitative proteomics</Keyword>
<Keyword MajorTopicYN="N">Vaccine</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year>
<Month>11</Month>
<Day>21</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2017</Year>
<Month>04</Month>
<Day>17</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2017</Year>
<Month>05</Month>
<Day>09</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2017</Year>
<Month>5</Month>
<Day>17</Day>
<Hour>6</Hour>
<Minute>0</Minute>
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<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>11</Month>
<Day>29</Day>
<Hour>6</Hour>
<Minute>0</Minute>
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<PubMedPubDate PubStatus="entrez"><Year>2017</Year>
<Month>5</Month>
<Day>17</Day>
<Hour>6</Hour>
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