Cleavage of hemagglutinin-bearing lentiviral pseudotypes and their use in the study of influenza virus persistence.
Identifieur interne : 000455 ( PubMed/Corpus ); précédent : 000454; suivant : 000456Cleavage of hemagglutinin-bearing lentiviral pseudotypes and their use in the study of influenza virus persistence.
Auteurs : Olivier Sawoo ; Amélie Dublineau ; Christophe Batéjat ; Paul Zhou ; Jean-Claude Manuguerra ; India LeclercqSource :
- PloS one [ 1932-6203 ] ; 2014.
English descriptors
- KwdEn :
- Animals, Dogs, HEK293 Cells, Hemagglutinin Glycoproteins, Influenza Virus (genetics), Hemagglutinin Glycoproteins, Influenza Virus (metabolism), Hot Temperature, Humans, Influenza A virus (classification), Influenza A virus (genetics), Influenza A virus (physiology), Lentivirus (genetics), Lentivirus (physiology), Madin Darby Canine Kidney Cells, Salinity, Stress, Physiological, Water.
- MESH :
- chemical , genetics : Hemagglutinin Glycoproteins, Influenza Virus.
- chemical , metabolism : Hemagglutinin Glycoproteins, Influenza Virus.
- classification : Influenza A virus.
- genetics : Influenza A virus, Lentivirus.
- physiology : Influenza A virus, Lentivirus.
- Animals, Dogs, HEK293 Cells, Hot Temperature, Humans, Madin Darby Canine Kidney Cells, Salinity, Stress, Physiological, Water.
Abstract
Influenza A viruses (IAVs) are a major cause of infectious respiratory human diseases and their transmission is dependent upon the environment. However, the role of environmental factors on IAV survival outside the host still raises many questions. In this study, we used lentiviral pseudotypes to study the influence of the hemagglutinin protein in IAV survival. High-titered and cleaved influenza-based lentiviral pseudoparticles, through the use of a combination of two proteases (HAT and TMPRSS2) were produced. Pseudoparticles bearing hemagglutinin proteins derived from different H1N1, H3N2 and H5N1 IAV strains were subjected to various environmental parameters over time and tested for viability through single-cycle infectivity assays. We showed that pseudotypes with different HAs have different persistence profiles in water as previously shown with IAVs. Our results also showed that pseudotypes derived from H1N1 pandemic virus survived longer than those derived from seasonal H1N1 virus from 1999, at high temperature and salinity, as previously shown with their viral counterparts. Similarly, increasing temperature and salinity had a negative effect on the survival of the H3N2 and H5N1 pseudotypes. These results showed that pseudotypes with the same lentiviral core, but which differ in their surface glycoproteins, survived differently outside the host, suggesting a role for the HA in virus stability.
DOI: 10.1371/journal.pone.0106192
PubMed: 25166303
Links to Exploration step
pubmed:25166303Le document en format XML
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University of Paris Diderot, Sorbonne Paris Cité (Cellule Pasteur), Paris, France.</nlm:affiliation></affiliation></author><author><name sortKey="Dublineau, Amelie" sort="Dublineau, Amelie" uniqKey="Dublineau A" first="Amélie" last="Dublineau">Amélie Dublineau</name><affiliation><nlm:affiliation>Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France.</nlm:affiliation></affiliation></author><author><name sortKey="Batejat, Christophe" sort="Batejat, Christophe" uniqKey="Batejat C" first="Christophe" last="Batéjat">Christophe Batéjat</name><affiliation><nlm:affiliation>Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France.</nlm:affiliation></affiliation></author><author><name sortKey="Zhou, Paul" sort="Zhou, Paul" uniqKey="Zhou P" first="Paul" last="Zhou">Paul Zhou</name><affiliation><nlm:affiliation>Institut Pasteur of Shanghai, Unit of Antiviral Immunity and Genetic Therapy, Chinese Academy of Sciences, Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Manuguerra, Jean Claude" sort="Manuguerra, Jean Claude" uniqKey="Manuguerra J" first="Jean-Claude" last="Manuguerra">Jean-Claude Manuguerra</name><affiliation><nlm:affiliation>Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France.</nlm:affiliation></affiliation></author><author><name sortKey="Leclercq, India" sort="Leclercq, India" uniqKey="Leclercq I" first="India" last="Leclercq">India Leclercq</name><affiliation><nlm:affiliation>Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France; University of Paris Diderot, Sorbonne Paris Cité (Cellule Pasteur), Paris, France.</nlm:affiliation></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Dogs</term><term>HEK293 Cells</term><term>Hemagglutinin Glycoproteins, Influenza Virus (genetics)</term><term>Hemagglutinin Glycoproteins, Influenza Virus (metabolism)</term><term>Hot Temperature</term><term>Humans</term><term>Influenza A virus (classification)</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (physiology)</term><term>Lentivirus (genetics)</term><term>Lentivirus (physiology)</term><term>Madin Darby Canine Kidney Cells</term><term>Salinity</term><term>Stress, Physiological</term><term>Water</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hemagglutinin Glycoproteins, Influenza Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Hemagglutinin Glycoproteins, Influenza Virus</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term><term>Lentivirus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A virus</term><term>Lentivirus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Dogs</term><term>HEK293 Cells</term><term>Hot Temperature</term><term>Humans</term><term>Madin Darby Canine Kidney Cells</term><term>Salinity</term><term>Stress, Physiological</term><term>Water</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Influenza A viruses (IAVs) are a major cause of infectious respiratory human diseases and their transmission is dependent upon the environment. However, the role of environmental factors on IAV survival outside the host still raises many questions. In this study, we used lentiviral pseudotypes to study the influence of the hemagglutinin protein in IAV survival. High-titered and cleaved influenza-based lentiviral pseudoparticles, through the use of a combination of two proteases (HAT and TMPRSS2) were produced. Pseudoparticles bearing hemagglutinin proteins derived from different H1N1, H3N2 and H5N1 IAV strains were subjected to various environmental parameters over time and tested for viability through single-cycle infectivity assays. We showed that pseudotypes with different HAs have different persistence profiles in water as previously shown with IAVs. Our results also showed that pseudotypes derived from H1N1 pandemic virus survived longer than those derived from seasonal H1N1 virus from 1999, at high temperature and salinity, as previously shown with their viral counterparts. Similarly, increasing temperature and salinity had a negative effect on the survival of the H3N2 and H5N1 pseudotypes. These results showed that pseudotypes with the same lentiviral core, but which differ in their surface glycoproteins, survived differently outside the host, suggesting a role for the HA in virus stability. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25166303</PMID><DateCompleted><Year>2015</Year><Month>11</Month><Day>17</Day></DateCompleted><DateRevised><Year>2019</Year><Month>02</Month><Day>23</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>8</Issue><PubDate><Year>2014</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS ONE</ISOAbbreviation></Journal><ArticleTitle>Cleavage of hemagglutinin-bearing lentiviral pseudotypes and their use in the study of influenza virus persistence.</ArticleTitle><Pagination><MedlinePgn>e106192</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0106192</ELocationID><Abstract><AbstractText>Influenza A viruses (IAVs) are a major cause of infectious respiratory human diseases and their transmission is dependent upon the environment. However, the role of environmental factors on IAV survival outside the host still raises many questions. In this study, we used lentiviral pseudotypes to study the influence of the hemagglutinin protein in IAV survival. High-titered and cleaved influenza-based lentiviral pseudoparticles, through the use of a combination of two proteases (HAT and TMPRSS2) were produced. Pseudoparticles bearing hemagglutinin proteins derived from different H1N1, H3N2 and H5N1 IAV strains were subjected to various environmental parameters over time and tested for viability through single-cycle infectivity assays. We showed that pseudotypes with different HAs have different persistence profiles in water as previously shown with IAVs. Our results also showed that pseudotypes derived from H1N1 pandemic virus survived longer than those derived from seasonal H1N1 virus from 1999, at high temperature and salinity, as previously shown with their viral counterparts. Similarly, increasing temperature and salinity had a negative effect on the survival of the H3N2 and H5N1 pseudotypes. These results showed that pseudotypes with the same lentiviral core, but which differ in their surface glycoproteins, survived differently outside the host, suggesting a role for the HA in virus stability. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sawoo</LastName><ForeName>Olivier</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France; University of Paris Diderot, Sorbonne Paris Cité (Cellule Pasteur), Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dublineau</LastName><ForeName>Amélie</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Batéjat</LastName><ForeName>Christophe</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Paul</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Institut Pasteur of Shanghai, Unit of Antiviral Immunity and Genetic Therapy, Chinese Academy of Sciences, Shanghai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Manuguerra</LastName><ForeName>Jean-Claude</ForeName><Initials>JC</Initials><AffiliationInfo><Affiliation>Institut Pasteur, Environment and Infectious Risks Research and Expertise Unit, Laboratory for Urgent Response to Biological Threats, Paris, France.</Affiliation></AffiliationInfo></Author><Author 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