Comparative transcriptome analysis reveals the role of p53 signalling pathway during red‐spotted grouper nervous necrosis virus infection in Lateolabrax japonicus brain cells
Identifieur interne : 000989 ( Ncbi/Checkpoint ); précédent : 000988; suivant : 000990Comparative transcriptome analysis reveals the role of p53 signalling pathway during red‐spotted grouper nervous necrosis virus infection in Lateolabrax japonicus brain cells
Auteurs : Yangxi Xiang ; Peng Jia ; Wei Liu ; Meisheng Yi ; Kuntong JiaSource :
- Journal of Fish Diseases [ 0140-7775 ] ; 2019.
Abstract
Nervous necrosis virus (NNV) is one of the fish pathogens that have caused mass mortalities of many marine and freshwater fishes in the world. To better comprehend the molecular immune mechanism of sea perch (
Url:
DOI: 10.1111/jfd.12960
PubMed: 30659619
PubMed Central: 7166548
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparative transcriptome analysis reveals the role of p53 signalling pathway during red‐spotted grouper nervous necrosis virus infection in <italic>Lateolabrax japonicus</italic> brain cells</title><author><name sortKey="Xiang, Yangxi" sort="Xiang, Yangxi" uniqKey="Xiang Y" first="Yangxi" last="Xiang">Yangxi Xiang</name><affiliation><nlm:aff id="jfd12960-aff-0001"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0002"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Jia, Peng" sort="Jia, Peng" uniqKey="Jia P" first="Peng" last="Jia">Peng Jia</name><affiliation><nlm:aff id="jfd12960-aff-0001"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0002"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Liu, Wei" sort="Liu, Wei" uniqKey="Liu W" first="Wei" last="Liu">Wei Liu</name><affiliation><nlm:aff id="jfd12960-aff-0001"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0002"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Yi, Meisheng" sort="Yi, Meisheng" uniqKey="Yi M" first="Meisheng" last="Yi">Meisheng Yi</name><affiliation><nlm:aff id="jfd12960-aff-0001"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0002"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Jia, Kuntong" sort="Jia, Kuntong" uniqKey="Jia K" first="Kuntong" last="Jia">Kuntong Jia</name><affiliation><nlm:aff id="jfd12960-aff-0001"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0002"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0003"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno 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grouper nervous necrosis virus infection in <italic>Lateolabrax japonicus</italic> brain cells</title><author><name sortKey="Xiang, Yangxi" sort="Xiang, Yangxi" uniqKey="Xiang Y" first="Yangxi" last="Xiang">Yangxi Xiang</name><affiliation><nlm:aff id="jfd12960-aff-0001"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0002"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Jia, Peng" sort="Jia, Peng" uniqKey="Jia P" first="Peng" last="Jia">Peng Jia</name><affiliation><nlm:aff id="jfd12960-aff-0001"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0002"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Liu, Wei" sort="Liu, Wei" uniqKey="Liu W" first="Wei" last="Liu">Wei Liu</name><affiliation><nlm:aff id="jfd12960-aff-0001"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0002"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Yi, Meisheng" sort="Yi, Meisheng" uniqKey="Yi M" first="Meisheng" last="Yi">Meisheng Yi</name><affiliation><nlm:aff id="jfd12960-aff-0001"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0002"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Jia, Kuntong" sort="Jia, Kuntong" uniqKey="Jia K" first="Kuntong" last="Jia">Kuntong Jia</name><affiliation><nlm:aff id="jfd12960-aff-0001"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0002"></nlm:aff></affiliation><affiliation><nlm:aff id="jfd12960-aff-0003"></nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Fish Diseases</title><idno type="ISSN">0140-7775</idno><idno type="eISSN">1365-2761</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Abstract</title><p>Nervous necrosis virus (NNV) is one of the fish pathogens that have caused mass mortalities of many marine and freshwater fishes in the world. To better comprehend the molecular immune mechanism of sea perch (<italic>Lateolabrax japonicus</italic>) against NNV infection, the comparative transcriptome analysis of red‐spotted grouper nervous necrosis virus (RGNNV)‐infected or mock‐infected <italic>L. japonicus</italic> brain (LJB) cells was performed via RNA sequencing technology. Here, 1,969 up‐regulated genes and 9,858 down‐regulated genes, which were widely implicated in immune response pathways, were identified. Furthermore, we confirmed that p53 signalling pathway was repressed at 48 hr post‐RGNNV infection, as indicated by up‐regulation of <italic>Mdm2</italic> and down‐regulation of <italic>p53</italic> and its downstream target genes, including <italic>Bax</italic>, <italic>Casp8</italic> and <italic>CytC</italic>. Overexpression of <italic>L. japonicus p53</italic> (<italic>Ljp53</italic>) significantly inhibited RGNNV replication and up‐regulated the expression of apoptosis‐related genes, whereas the down‐regulation caused by pifithrin‐α led to the opposite effect, suggesting Ljp53 might promote cell apoptosis to repress virus replication. Luciferase assay indicated that Ljp53 could enhance the promoter activities of zebrafish interferon (IFN)1, indicating that Ljp53 could exert its anti‐RGNNV activities by enforcing the type I IFN response. This study revealed the potential antiviral role of p53 during NNV infection.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Jia, Kuntong" sort="Jia, Kuntong" uniqKey="Jia K" first="Kuntong" last="Jia">Kuntong Jia</name><name sortKey="Jia, Peng" sort="Jia, Peng" uniqKey="Jia P" first="Peng" last="Jia">Peng Jia</name><name sortKey="Liu, Wei" sort="Liu, Wei" uniqKey="Liu W" first="Wei" last="Liu">Wei Liu</name><name sortKey="Xiang, Yangxi" sort="Xiang, Yangxi" uniqKey="Xiang Y" first="Yangxi" last="Xiang">Yangxi Xiang</name><name sortKey="Yi, Meisheng" sort="Yi, Meisheng" uniqKey="Yi M" first="Meisheng" last="Yi">Meisheng Yi</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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