Region‐resolved proteomics profiling of monkey heart
Identifieur interne : 000988 ( Ncbi/Checkpoint ); précédent : 000987; suivant : 000989Region‐resolved proteomics profiling of monkey heart
Auteurs : Hao-Liang Hu ; Yu Kang ; Yong Zeng ; Ming Zhang ; Qiong Liao ; Ming-Qiang Rong ; Qin Zhang ; Ren LaiSource :
- Journal of Cellular Physiology [ 0021-9541 ] ; 2019.
Abstract
Nonhuman primates (NHPs) play an indispensable role in biomedical research because of their similarities in genetics, physiological, and neurological function to humans. Proteomics profiling of monkey heart could reveal significant cardiac biomarkers and help us to gain a better understanding of the pathogenesis of heart disease. However, the proteomic study of monkey heart is relatively lacking. Here, we performed the proteomics profiling of the normal monkey heart by measuring three major anatomical regions (vessels, valves, and chambers) based on iTRAQ‐coupled LC‐MS/MS analysis. Over 3,200 proteins were identified and quantified from three heart tissue samples. Furthermore, multiple bioinformatics analyses such as gene ontology analysis, protein–protein interaction analysis, and gene‐diseases association were used to investigate biological network of those proteins from each area. More than 60 genes in three heart regions are implicated with heart diseases such as hypertrophic cardiomyopathy, heart failure, and myocardial infarction. These genes associated with heart disease are mainly enriched in citrate cycle, amino acid degradation, and glycolysis pathway. At the anatomical level, the revelation of molecular characteristics of the healthy monkey heart would be an important starting point to investigate heart disease. As a unique resource, this study can serve as a reference map for future in‐depth research on cardiac disease‐related NHP model and novel biomarkers of cardiac injury.
Url:
DOI: 10.1002/jcp.28052
PubMed: 30644093
PubMed Central: 7166496
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000723
- to stream Pmc, to step Curation: 000723
- to stream Pmc, to step Checkpoint: 000710
- to stream Ncbi, to step Merge: 000988
- to stream Ncbi, to step Curation: 000988
Links to Exploration step
PMC:7166496Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Region‐resolved proteomics profiling of monkey heart</title>
<author><name sortKey="Hu, Hao Iang" sort="Hu, Hao Iang" uniqKey="Hu H" first="Hao-Liang" last="Hu">Hao-Liang Hu</name>
<affiliation><nlm:aff id="jcp28052-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kang, Yu" sort="Kang, Yu" uniqKey="Kang Y" first="Yu" last="Kang">Yu Kang</name>
<affiliation><nlm:aff id="jcp28052-aff-0002"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zeng, Yong" sort="Zeng, Yong" uniqKey="Zeng Y" first="Yong" last="Zeng">Yong Zeng</name>
<affiliation><nlm:aff id="jcp28052-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhang, Ming" sort="Zhang, Ming" uniqKey="Zhang M" first="Ming" last="Zhang">Ming Zhang</name>
<affiliation><nlm:aff id="jcp28052-aff-0003"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liao, Qiong" sort="Liao, Qiong" uniqKey="Liao Q" first="Qiong" last="Liao">Qiong Liao</name>
<affiliation><nlm:aff id="jcp28052-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Rong, Ming Iang" sort="Rong, Ming Iang" uniqKey="Rong M" first="Ming-Qiang" last="Rong">Ming-Qiang Rong</name>
<affiliation><nlm:aff id="jcp28052-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhang, Qin" sort="Zhang, Qin" uniqKey="Zhang Q" first="Qin" last="Zhang">Qin Zhang</name>
<affiliation><nlm:aff id="jcp28052-aff-0002"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lai, Ren" sort="Lai, Ren" uniqKey="Lai R" first="Ren" last="Lai">Ren Lai</name>
<affiliation><nlm:aff id="jcp28052-aff-0003"></nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">30644093</idno>
<idno type="pmc">7166496</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7166496</idno>
<idno type="RBID">PMC:7166496</idno>
<idno type="doi">10.1002/jcp.28052</idno>
<date when="2019">2019</date>
<idno type="wicri:Area/Pmc/Corpus">000723</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000723</idno>
<idno type="wicri:Area/Pmc/Curation">000723</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000723</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000710</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000710</idno>
<idno type="wicri:Area/Ncbi/Merge">000988</idno>
<idno type="wicri:Area/Ncbi/Curation">000988</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000988</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Region‐resolved proteomics profiling of monkey heart</title>
<author><name sortKey="Hu, Hao Iang" sort="Hu, Hao Iang" uniqKey="Hu H" first="Hao-Liang" last="Hu">Hao-Liang Hu</name>
<affiliation><nlm:aff id="jcp28052-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kang, Yu" sort="Kang, Yu" uniqKey="Kang Y" first="Yu" last="Kang">Yu Kang</name>
<affiliation><nlm:aff id="jcp28052-aff-0002"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zeng, Yong" sort="Zeng, Yong" uniqKey="Zeng Y" first="Yong" last="Zeng">Yong Zeng</name>
<affiliation><nlm:aff id="jcp28052-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhang, Ming" sort="Zhang, Ming" uniqKey="Zhang M" first="Ming" last="Zhang">Ming Zhang</name>
<affiliation><nlm:aff id="jcp28052-aff-0003"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liao, Qiong" sort="Liao, Qiong" uniqKey="Liao Q" first="Qiong" last="Liao">Qiong Liao</name>
<affiliation><nlm:aff id="jcp28052-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Rong, Ming Iang" sort="Rong, Ming Iang" uniqKey="Rong M" first="Ming-Qiang" last="Rong">Ming-Qiang Rong</name>
<affiliation><nlm:aff id="jcp28052-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhang, Qin" sort="Zhang, Qin" uniqKey="Zhang Q" first="Qin" last="Zhang">Qin Zhang</name>
<affiliation><nlm:aff id="jcp28052-aff-0002"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lai, Ren" sort="Lai, Ren" uniqKey="Lai R" first="Ren" last="Lai">Ren Lai</name>
<affiliation><nlm:aff id="jcp28052-aff-0003"></nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Cellular Physiology</title>
<idno type="ISSN">0021-9541</idno>
<idno type="eISSN">1097-4652</idno>
<imprint><date when="2019">2019</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>Abstract</title>
<p>Nonhuman primates (NHPs) play an indispensable role in biomedical research because of their similarities in genetics, physiological, and neurological function to humans. Proteomics profiling of monkey heart could reveal significant cardiac biomarkers and help us to gain a better understanding of the pathogenesis of heart disease. However, the proteomic study of monkey heart is relatively lacking. Here, we performed the proteomics profiling of the normal monkey heart by measuring three major anatomical regions (vessels, valves, and chambers) based on iTRAQ‐coupled LC‐MS/MS analysis. Over 3,200 proteins were identified and quantified from three heart tissue samples. Furthermore, multiple bioinformatics analyses such as gene ontology analysis, protein–protein interaction analysis, and gene‐diseases association were used to investigate biological network of those proteins from each area. More than 60 genes in three heart regions are implicated with heart diseases such as hypertrophic cardiomyopathy, heart failure, and myocardial infarction. These genes associated with heart disease are mainly enriched in citrate cycle, amino acid degradation, and glycolysis pathway. At the anatomical level, the revelation of molecular characteristics of the healthy monkey heart would be an important starting point to investigate heart disease. As a unique resource, this study can serve as a reference map for future in‐depth research on cardiac disease‐related NHP model and novel biomarkers of cardiac injury.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl></listBibl>
</div1>
</back>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Hu, Hao Iang" sort="Hu, Hao Iang" uniqKey="Hu H" first="Hao-Liang" last="Hu">Hao-Liang Hu</name>
<name sortKey="Kang, Yu" sort="Kang, Yu" uniqKey="Kang Y" first="Yu" last="Kang">Yu Kang</name>
<name sortKey="Lai, Ren" sort="Lai, Ren" uniqKey="Lai R" first="Ren" last="Lai">Ren Lai</name>
<name sortKey="Liao, Qiong" sort="Liao, Qiong" uniqKey="Liao Q" first="Qiong" last="Liao">Qiong Liao</name>
<name sortKey="Rong, Ming Iang" sort="Rong, Ming Iang" uniqKey="Rong M" first="Ming-Qiang" last="Rong">Ming-Qiang Rong</name>
<name sortKey="Zeng, Yong" sort="Zeng, Yong" uniqKey="Zeng Y" first="Yong" last="Zeng">Yong Zeng</name>
<name sortKey="Zhang, Ming" sort="Zhang, Ming" uniqKey="Zhang M" first="Ming" last="Zhang">Ming Zhang</name>
<name sortKey="Zhang, Qin" sort="Zhang, Qin" uniqKey="Zhang Q" first="Qin" last="Zhang">Qin Zhang</name>
</noCountry>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/StressCovidV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000988 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000988 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= StressCovidV1 |flux= Ncbi |étape= Checkpoint |type= RBID |clé= PMC:7166496 |texte= Region‐resolved proteomics profiling of monkey heart }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:30644093" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a StressCovidV1
This area was generated with Dilib version V0.6.33. |