Identification of 23-(s)-2-amino-3-phenylpropanoyl-silybin as an antiviral agent for influenza A virus infection in vitro and in vivo.
Identifieur interne : 001075 ( Main/Exploration ); précédent : 001074; suivant : 001076Identification of 23-(s)-2-amino-3-phenylpropanoyl-silybin as an antiviral agent for influenza A virus infection in vitro and in vivo.
Auteurs : Jian-Ping Dai [République populaire de Chine] ; Li-Qi Wu ; Rui Li ; Xiang-Feng Zhao ; Qian-Ying Wan ; Xiao-Xuan Chen ; Wei-Zhong Li ; Ge-Fei Wang ; Kang-Sheng LiSource :
- Antimicrobial agents and chemotherapy [ 1098-6596 ] ; 2013.
Descripteurs français
- KwdFr :
- Animaux, Antiviraux (isolement et purification), Antiviraux (pharmacologie), Antiviraux (synthèse chimique), Autophagie (), Cellules Vero, Cellules rénales canines Madin-Darby, Chardon-Marie (), Chiens, Extraits de plantes (), Humains, Mitogen-Activated Protein Kinases (antagonistes et inhibiteurs), Mitogen-Activated Protein Kinases (génétique), Mitogen-Activated Protein Kinases (métabolisme), Multimérisation de protéines (), Petit ARN interférent (génétique), Petit ARN interférent (métabolisme), Petites protéines modificatrices apparentées à l'ubiquitine (antagonistes et inhibiteurs), Petites protéines modificatrices apparentées à l'ubiquitine (génétique), Petites protéines modificatrices apparentées à l'ubiquitine (métabolisme), Plasmides, Protéine-12 associée à l'autophagie, Protéine-5 associée à l'autophagie, Protéines associées aux microtubules (antagonistes et inhibiteurs), Protéines associées aux microtubules (génétique), Protéines associées aux microtubules (métabolisme), Protéines associées à l'autophagie, Protéines de transport (antagonistes et inhibiteurs), Protéines de transport (génétique), Protéines de transport (métabolisme), Régulation de l'expression des gènes, Silibinine (analogues et dérivés), Silibinine (isolement et purification), Silibinine (pharmacologie), Silibinine (synthèse chimique), Sous-type H1N1 du virus de la grippe A (), Sous-type H1N1 du virus de la grippe A (croissance et développement), Stress oxydatif (), Tests de criblage à haut débit, Transduction du signal.
- MESH :
- analogues et dérivés : Silibinine.
- antagonistes et inhibiteurs : Mitogen-Activated Protein Kinases, Petites protéines modificatrices apparentées à l'ubiquitine, Protéines associées aux microtubules, Protéines de transport.
- croissance et développement : Sous-type H1N1 du virus de la grippe A.
- génétique : Mitogen-Activated Protein Kinases, Petit ARN interférent, Petites protéines modificatrices apparentées à l'ubiquitine, Protéines associées aux microtubules, Protéines de transport.
- isolement et purification : Antiviraux, Silibinine.
- métabolisme : Mitogen-Activated Protein Kinases, Petit ARN interférent, Petites protéines modificatrices apparentées à l'ubiquitine, Protéines associées aux microtubules, Protéines de transport.
- pharmacologie : Antiviraux, Silibinine.
- synthèse chimique : Antiviraux, Silibinine.
- Animaux, Autophagie, Cellules Vero, Cellules rénales canines Madin-Darby, Chardon-Marie, Chiens, Extraits de plantes, Humains, Multimérisation de protéines, Plasmides, Protéine-12 associée à l'autophagie, Protéine-5 associée à l'autophagie, Protéines associées à l'autophagie, Régulation de l'expression des gènes, Sous-type H1N1 du virus de la grippe A, Stress oxydatif, Tests de criblage à haut débit, Transduction du signal.
English descriptors
- KwdEn :
- Animals, Antiviral Agents (chemical synthesis), Antiviral Agents (isolation & purification), Antiviral Agents (pharmacology), Autophagy (drug effects), Autophagy-Related Protein 12, Autophagy-Related Protein 5, Autophagy-Related Proteins, Carrier Proteins (antagonists & inhibitors), Carrier Proteins (genetics), Carrier Proteins (metabolism), Chlorocebus aethiops, Dogs, Gene Expression Regulation, High-Throughput Screening Assays, Humans, Influenza A Virus, H1N1 Subtype (drug effects), Influenza A Virus, H1N1 Subtype (growth & development), Madin Darby Canine Kidney Cells, Microtubule-Associated Proteins (antagonists & inhibitors), Microtubule-Associated Proteins (genetics), Microtubule-Associated Proteins (metabolism), Milk Thistle (chemistry), Mitogen-Activated Protein Kinases (antagonists & inhibitors), Mitogen-Activated Protein Kinases (genetics), Mitogen-Activated Protein Kinases (metabolism), Oxidative Stress (drug effects), Plant Extracts (chemistry), Plasmids, Protein Multimerization (drug effects), RNA, Small Interfering (genetics), RNA, Small Interfering (metabolism), Signal Transduction, Silymarin (analogs & derivatives), Silymarin (chemical synthesis), Silymarin (isolation & purification), Silymarin (pharmacology), Small Ubiquitin-Related Modifier Proteins (antagonists & inhibitors), Small Ubiquitin-Related Modifier Proteins (genetics), Small Ubiquitin-Related Modifier Proteins (metabolism), Vero Cells.
- MESH :
- chemical , analogs & derivatives : Silymarin.
- chemical , antagonists & inhibitors : Carrier Proteins, Microtubule-Associated Proteins, Mitogen-Activated Protein Kinases, Small Ubiquitin-Related Modifier Proteins.
- chemical , chemical synthesis : Antiviral Agents, Silymarin.
- chemical , chemistry : Plant Extracts.
- chemical , genetics : Carrier Proteins, Microtubule-Associated Proteins, Mitogen-Activated Protein Kinases, RNA, Small Interfering, Small Ubiquitin-Related Modifier Proteins.
- chemical , isolation & purification : Antiviral Agents, Silymarin.
- chemical , metabolism : Carrier Proteins, Microtubule-Associated Proteins, Mitogen-Activated Protein Kinases, RNA, Small Interfering, Small Ubiquitin-Related Modifier Proteins.
- chemical , pharmacology : Antiviral Agents, Silymarin.
- chemistry : Milk Thistle.
- drug effects : Autophagy, Influenza A Virus, H1N1 Subtype, Oxidative Stress, Protein Multimerization.
- growth & development : Influenza A Virus, H1N1 Subtype.
- Animals, Autophagy-Related Protein 12, Autophagy-Related Protein 5, Autophagy-Related Proteins, Chlorocebus aethiops, Dogs, Gene Expression Regulation, High-Throughput Screening Assays, Humans, Madin Darby Canine Kidney Cells, Plasmids, Signal Transduction, Vero Cells.
Abstract
It has been reported that autophagy is involved in the replication of many viruses. In this study, we screened 89 medicinal plants, using an assay based on the inhibition of the formation of the Atg12-Atg5/Atg16 heterotrimer, an important regulator of autophagy, and selected Silybum marianum L. for further study. An antiviral assay indicated that silybin (S0), the major active compound of S. marianum L., can inhibit influenza A virus (IAV) infection. We later synthesized 5 silybin derivatives (S1 through S5) and found that 23-(S)-2-amino-3-phenylpropanoyl-silybin (S3) had the best activity. When we compared the polarities of the substituent groups, we found that the hydrophobicity of the substituent groups was positively correlated with their activities. We further studied the mechanisms of action of these compounds and determined that S0 and S3 also inhibited both the formation of the Atg12-Atg5/Atg16 heterotrimer and the elevated autophagy induced by IAV infection. In addition, we found that S0 and S3 could inhibit several components induced by IAV infection, including oxidative stress, the activation of extracellular signal-regulated kinase (ERK)/p38 mitogen-activated protein kinase (MAPK) and IκB kinase (IKK) pathways, and the expression of autophagic genes, especially Atg7 and Atg3. All of these components have been reported to be related to the formation of the Atg12-Atg5/Atg16 heterotrimer, which might validate our screening strategy. Finally, we demonstrated that S3 can significantly reduce influenza virus replication and the associated mortality in infected mice. In conclusion, we identified 23-(S)-2-amino-3-phenylpropanoyl-silybin as a promising inhibitor of IAV infection.
DOI: 10.1128/AAC.00759-13
PubMed: 23836164
Affiliations:
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in vitro and in vivo.</title><author><name sortKey="Dai, Jian Ping" sort="Dai, Jian Ping" uniqKey="Dai J" first="Jian-Ping" last="Dai">Jian-Ping Dai</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong</wicri:regionArea><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Li Qi" sort="Wu, Li Qi" uniqKey="Wu L" first="Li-Qi" last="Wu">Li-Qi Wu</name></author><author><name sortKey="Li, Rui" sort="Li, Rui" uniqKey="Li R" first="Rui" last="Li">Rui Li</name></author><author><name sortKey="Zhao, Xiang Feng" sort="Zhao, Xiang Feng" uniqKey="Zhao X" first="Xiang-Feng" last="Zhao">Xiang-Feng Zhao</name></author><author><name sortKey="Wan, Qian Ying" sort="Wan, Qian Ying" uniqKey="Wan Q" first="Qian-Ying" last="Wan">Qian-Ying Wan</name></author><author><name sortKey="Chen, Xiao Xuan" sort="Chen, Xiao Xuan" uniqKey="Chen X" first="Xiao-Xuan" last="Chen">Xiao-Xuan Chen</name></author><author><name sortKey="Li, Wei Zhong" sort="Li, Wei Zhong" uniqKey="Li W" first="Wei-Zhong" last="Li">Wei-Zhong Li</name></author><author><name sortKey="Wang, Ge Fei" sort="Wang, Ge Fei" uniqKey="Wang G" first="Ge-Fei" last="Wang">Ge-Fei Wang</name></author><author><name sortKey="Li, Kang Sheng" sort="Li, Kang Sheng" uniqKey="Li K" first="Kang-Sheng" last="Li">Kang-Sheng Li</name></author></analytic><series><title level="j">Antimicrobial agents and chemotherapy</title><idno type="eISSN">1098-6596</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antiviral Agents (chemical synthesis)</term><term>Antiviral Agents (isolation & purification)</term><term>Antiviral Agents (pharmacology)</term><term>Autophagy (drug effects)</term><term>Autophagy-Related Protein 12</term><term>Autophagy-Related Protein 5</term><term>Autophagy-Related Proteins</term><term>Carrier Proteins (antagonists & inhibitors)</term><term>Carrier Proteins (genetics)</term><term>Carrier Proteins (metabolism)</term><term>Chlorocebus aethiops</term><term>Dogs</term><term>Gene Expression Regulation</term><term>High-Throughput Screening Assays</term><term>Humans</term><term>Influenza A Virus, H1N1 Subtype (drug effects)</term><term>Influenza A Virus, H1N1 Subtype (growth & development)</term><term>Madin Darby Canine Kidney Cells</term><term>Microtubule-Associated Proteins (antagonists & inhibitors)</term><term>Microtubule-Associated Proteins (genetics)</term><term>Microtubule-Associated Proteins (metabolism)</term><term>Milk Thistle (chemistry)</term><term>Mitogen-Activated Protein Kinases (antagonists & inhibitors)</term><term>Mitogen-Activated Protein Kinases (genetics)</term><term>Mitogen-Activated Protein Kinases (metabolism)</term><term>Oxidative Stress (drug effects)</term><term>Plant Extracts (chemistry)</term><term>Plasmids</term><term>Protein Multimerization (drug effects)</term><term>RNA, Small Interfering (genetics)</term><term>RNA, Small Interfering (metabolism)</term><term>Signal Transduction</term><term>Silymarin (analogs & derivatives)</term><term>Silymarin (chemical synthesis)</term><term>Silymarin (isolation & purification)</term><term>Silymarin (pharmacology)</term><term>Small Ubiquitin-Related Modifier Proteins (antagonists & inhibitors)</term><term>Small Ubiquitin-Related Modifier Proteins (genetics)</term><term>Small Ubiquitin-Related Modifier Proteins (metabolism)</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Antiviraux (isolement et purification)</term><term>Antiviraux (pharmacologie)</term><term>Antiviraux (synthèse chimique)</term><term>Autophagie ()</term><term>Cellules Vero</term><term>Cellules rénales canines Madin-Darby</term><term>Chardon-Marie ()</term><term>Chiens</term><term>Extraits de plantes ()</term><term>Humains</term><term>Mitogen-Activated Protein Kinases (antagonistes et inhibiteurs)</term><term>Mitogen-Activated Protein Kinases (génétique)</term><term>Mitogen-Activated Protein Kinases (métabolisme)</term><term>Multimérisation de protéines ()</term><term>Petit ARN interférent (génétique)</term><term>Petit ARN interférent (métabolisme)</term><term>Petites protéines modificatrices apparentées à l'ubiquitine (antagonistes et inhibiteurs)</term><term>Petites protéines modificatrices apparentées à l'ubiquitine (génétique)</term><term>Petites protéines modificatrices apparentées à l'ubiquitine (métabolisme)</term><term>Plasmides</term><term>Protéine-12 associée à l'autophagie</term><term>Protéine-5 associée à l'autophagie</term><term>Protéines associées aux microtubules (antagonistes et inhibiteurs)</term><term>Protéines associées aux microtubules (génétique)</term><term>Protéines associées aux microtubules (métabolisme)</term><term>Protéines associées à l'autophagie</term><term>Protéines de transport (antagonistes et inhibiteurs)</term><term>Protéines de transport (génétique)</term><term>Protéines de transport (métabolisme)</term><term>Régulation de l'expression des gènes</term><term>Silibinine (analogues et dérivés)</term><term>Silibinine (isolement et purification)</term><term>Silibinine (pharmacologie)</term><term>Silibinine (synthèse chimique)</term><term>Sous-type H1N1 du virus de la grippe A ()</term><term>Sous-type H1N1 du virus de la grippe A (croissance et développement)</term><term>Stress oxydatif ()</term><term>Tests de criblage à haut débit</term><term>Transduction du signal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Silymarin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Carrier Proteins</term><term>Microtubule-Associated Proteins</term><term>Mitogen-Activated Protein Kinases</term><term>Small Ubiquitin-Related Modifier Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Antiviral Agents</term><term>Silymarin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Extracts</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carrier Proteins</term><term>Microtubule-Associated Proteins</term><term>Mitogen-Activated Protein Kinases</term><term>RNA, Small Interfering</term><term>Small Ubiquitin-Related Modifier Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antiviral Agents</term><term>Silymarin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carrier Proteins</term><term>Microtubule-Associated Proteins</term><term>Mitogen-Activated Protein Kinases</term><term>RNA, Small Interfering</term><term>Small Ubiquitin-Related Modifier Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term><term>Silymarin</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Silibinine</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Mitogen-Activated Protein Kinases</term><term>Petites protéines modificatrices apparentées à l'ubiquitine</term><term>Protéines associées aux microtubules</term><term>Protéines de transport</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Milk Thistle</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Autophagy</term><term>Influenza A Virus, H1N1 Subtype</term><term>Oxidative Stress</term><term>Protein Multimerization</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Mitogen-Activated Protein Kinases</term><term>Petit ARN interférent</term><term>Petites protéines modificatrices apparentées à l'ubiquitine</term><term>Protéines associées aux microtubules</term><term>Protéines de transport</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antiviraux</term><term>Silibinine</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Mitogen-Activated Protein Kinases</term><term>Petit ARN interférent</term><term>Petites protéines modificatrices apparentées à l'ubiquitine</term><term>Protéines associées aux microtubules</term><term>Protéines de transport</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antiviraux</term><term>Silibinine</term></keywords><keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Antiviraux</term><term>Silibinine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Autophagy-Related Protein 12</term><term>Autophagy-Related Protein 5</term><term>Autophagy-Related Proteins</term><term>Chlorocebus aethiops</term><term>Dogs</term><term>Gene Expression Regulation</term><term>High-Throughput Screening Assays</term><term>Humans</term><term>Madin Darby Canine Kidney Cells</term><term>Plasmids</term><term>Signal Transduction</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Autophagie</term><term>Cellules Vero</term><term>Cellules rénales canines Madin-Darby</term><term>Chardon-Marie</term><term>Chiens</term><term>Extraits de plantes</term><term>Humains</term><term>Multimérisation de protéines</term><term>Plasmides</term><term>Protéine-12 associée à l'autophagie</term><term>Protéine-5 associée à l'autophagie</term><term>Protéines associées à l'autophagie</term><term>Régulation de l'expression des gènes</term><term>Sous-type H1N1 du virus de la grippe A</term><term>Stress oxydatif</term><term>Tests de criblage à haut débit</term><term>Transduction du signal</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">It has been reported that autophagy is involved in the replication of many viruses. In this study, we screened 89 medicinal plants, using an assay based on the inhibition of the formation of the Atg12-Atg5/Atg16 heterotrimer, an important regulator of autophagy, and selected Silybum marianum L. for further study. An antiviral assay indicated that silybin (S0), the major active compound of S. marianum L., can inhibit influenza A virus (IAV) infection. We later synthesized 5 silybin derivatives (S1 through S5) and found that 23-(S)-2-amino-3-phenylpropanoyl-silybin (S3) had the best activity. When we compared the polarities of the substituent groups, we found that the hydrophobicity of the substituent groups was positively correlated with their activities. We further studied the mechanisms of action of these compounds and determined that S0 and S3 also inhibited both the formation of the Atg12-Atg5/Atg16 heterotrimer and the elevated autophagy induced by IAV infection. In addition, we found that S0 and S3 could inhibit several components induced by IAV infection, including oxidative stress, the activation of extracellular signal-regulated kinase (ERK)/p38 mitogen-activated protein kinase (MAPK) and IκB kinase (IKK) pathways, and the expression of autophagic genes, especially Atg7 and Atg3. All of these components have been reported to be related to the formation of the Atg12-Atg5/Atg16 heterotrimer, which might validate our screening strategy. Finally, we demonstrated that S3 can significantly reduce influenza virus replication and the associated mortality in infected mice. In conclusion, we identified 23-(S)-2-amino-3-phenylpropanoyl-silybin as a promising inhibitor of IAV infection. </div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chen, Xiao Xuan" sort="Chen, Xiao Xuan" uniqKey="Chen X" first="Xiao-Xuan" last="Chen">Xiao-Xuan Chen</name><name sortKey="Li, Kang Sheng" sort="Li, Kang Sheng" uniqKey="Li K" first="Kang-Sheng" last="Li">Kang-Sheng Li</name><name sortKey="Li, Rui" sort="Li, Rui" uniqKey="Li R" first="Rui" last="Li">Rui Li</name><name sortKey="Li, Wei Zhong" sort="Li, Wei Zhong" uniqKey="Li W" first="Wei-Zhong" last="Li">Wei-Zhong Li</name><name sortKey="Wan, Qian Ying" sort="Wan, Qian Ying" uniqKey="Wan Q" first="Qian-Ying" last="Wan">Qian-Ying Wan</name><name sortKey="Wang, Ge Fei" sort="Wang, Ge Fei" uniqKey="Wang G" first="Ge-Fei" last="Wang">Ge-Fei Wang</name><name sortKey="Wu, Li Qi" sort="Wu, Li Qi" uniqKey="Wu L" first="Li-Qi" last="Wu">Li-Qi Wu</name><name sortKey="Zhao, Xiang Feng" sort="Zhao, Xiang Feng" uniqKey="Zhao X" first="Xiang-Feng" last="Zhao">Xiang-Feng Zhao</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Dai, Jian Ping" sort="Dai, Jian Ping" uniqKey="Dai J" first="Jian-Ping" last="Dai">Jian-Ping Dai</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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